ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.95 0.92 0.87 0.88 – 0.75 0.75 0.74 0.68 0.97 0.98 0.95 – – – 0.93 0.95 0.93 0.8 0.77 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.08 0.1 0.08 – 0.34 0.26 0.3 0.34 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.11 0.09 – 0.07 0.12 0.06 0.07 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 1 7 0 0 3
Bud 1 1 0 1 2 0 3 – 3 3 10 10 0 0 0 – – – 0 1 6 3 8 5
Bud Neck 0 1 0 0 1 0 0 – 1 3 0 0 0 0 0 – – – 0 1 1 0 1 1
Bud Site 0 0 0 0 0 0 0 – 1 0 0 4 0 0 0 – – – – – – – – –
Cell Periphery 1 3 7 4 1 3 4 – 1 6 9 8 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 299 620 665 424 406 410 512 – 389 425 329 393 169 225 247 – – – 295 597 614 147 217 169
Endoplasmic Reticulum 1 2 2 0 1 0 1 – 1 0 0 0 0 0 1 – – – 0 0 4 1 2 1
Endosome 0 0 0 2 1 2 5 – 1 6 4 2 0 1 0 – – – 3 4 3 3 11 3
Golgi 1 1 4 0 1 0 1 – 0 0 0 0 0 0 0 – – – 1 3 1 2 1 1
Mitochondria 1 5 1 18 35 48 49 – 177 147 135 198 1 0 1 – – – 1 5 7 1 3 4
Nucleus 0 4 0 1 4 7 5 – 14 10 11 13 5 0 9 – – – 1 2 1 8 7 6
Nuclear Periphery 0 0 0 0 2 1 0 – 1 1 1 0 0 0 0 – – – 0 0 0 0 2 1
Nucleolus 0 0 1 0 1 0 2 – 0 5 0 1 0 0 0 – – – 1 1 0 6 6 6
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 1 0 0 0 0
SpindlePole 0 0 1 1 0 0 2 – 0 2 0 1 0 0 0 – – – 1 1 4 2 7 6
Vac/Vac Membrane 5 6 15 18 18 52 52 – 37 68 27 41 2 4 4 – – – 9 4 0 2 6 6
Unique Cell Count 304 630 678 444 443 470 580 519 567 444 574 175 230 260 317 629 662 186 284 226
Labelled Cell Count 309 644 697 469 473 523 636 626 676 526 671 177 230 262 317 629 662 186 284 226


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.4 6.9 6.2 5.5 5.4 5.4 – 4.4 5.0 4.9 4.9 6.8 6.5 6.3 – – – 7.9 8.6 8.5
Std Deviation (1e-4) 0.7 0.8 1.5 1.7 1.6 1.3 1.6 – 1.4 1.3 1.2 1.3 1.4 1.4 1.6 – – – 2.7 2.5 2.3
Intensity Change (Log2) – – – -0.16 -0.32 -0.36 -0.35 – -0.65 -0.45 -0.51 -0.5 -0.02 -0.07 -0.13 – – – 0.21 0.33 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 -0.7 – 0 0 0 0 0 0 0 – – –
Cytoplasm -2.5 -5.1 -7.4 -7.1 – -12.2 -12.3 -12.4 -14.4 -1.2 -0.2 -2.6 – – –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Mitochondria 5.0 7.2 8.3 7.5 – 0 14.0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 – 4.3 0 0 3.9 0 0 0 – – –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Vacuole 1.8 1.8 6.3 5.3 – 4.1 6.9 3.3 4.2 0 0 -0.7 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1662 6.81 6.367 6.7505 6.402 6.4437 6.092 6.6193 6.2003 6.4896 5.6179 6.6688 3.9201 4.0036 3.5067 4.6505 4.4692 3.8996
Actin 0.0572 0.0013 0.0137 0.0143 0.0056 0.0127 0.0482 0.001 0.0216 0.1219 0.017 0.0064 0.0309 0.0065 0.0024 0.0041 0.015 0.0027
Bud 0.013 0.0192 0.0526 0.0126 0.0198 0.0195 0.0109 0.0134 0.0019 0.001 0.0032 0.0008 0.0029 0.0007 0.0005 0.0002 0.0003 0.0007
Bud Neck 0.0024 0.0006 0.0062 0.0011 0.0007 0.0052 0.0165 0.0003 0.0017 0.0061 0.0018 0.0012 0.0013 0.0003 0.0003 0.0011 0.0016 0.0011
Bud Periphery 0.0102 0.0061 0.0186 0.0102 0.0069 0.0112 0.0042 0.0073 0.0023 0.0012 0.0062 0.0009 0.005 0.0006 0.0008 0.0002 0.0003 0.0008
Bud Site 0.0123 0.0374 0.0227 0.005 0.0047 0.0016 0.013 0.0064 0.0088 0.0635 0.0136 0.0005 0.0016 0.0068 0.0004 0.0011 0.0003 0.0007
Cell Periphery 0.0022 0.0011 0.0008 0.0005 0.0002 0.0005 0.0004 0.0001 0.0003 0.0026 0.0006 0.0001 0.0002 0.0001 0.0001 0.0005 0.0001 0.0001
Cytoplasm 0.4932 0.6304 0.5288 0.4359 0.5894 0.6021 0.5546 0.7699 0.6433 0.4384 0.5945 0.6731 0.5598 0.7546 0.596 0.5453 0.5337 0.6533
Cytoplasmic Foci 0.036 0.0108 0.0282 0.0355 0.0309 0.0296 0.0219 0.007 0.0163 0.0113 0.0241 0.0187 0.0308 0.0086 0.013 0.0397 0.0133 0.0119
Eisosomes 0.0009 0.0001 0.0002 0.0003 0.0001 0.0001 0.0003 0 0.0003 0.0009 0.0003 0 0.0002 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.009 0.0023 0.0034 0.0047 0.0027 0.0035 0.0039 0.0034 0.0052 0.0011 0.0078 0.0037 0.0045 0.0028 0.0032 0.0007 0.0021 0.002
Endosome 0.024 0.0018 0.0111 0.0298 0.0224 0.0095 0.0094 0.0029 0.0112 0.0052 0.0251 0.0139 0.0186 0.0023 0.0072 0.0173 0.003 0.0128
Golgi 0.0138 0.0003 0.0054 0.0169 0.0091 0.0063 0.0024 0.0002 0.0025 0.0065 0.0077 0.0069 0.0109 0.0006 0.0033 0.0107 0.0006 0.0028
Lipid Particles 0.0076 0.0002 0.0037 0.0228 0.0077 0.0043 0.0021 0.0002 0.0032 0.0043 0.0013 0.006 0.0134 0.0003 0.0114 0.0183 0.0005 0.0052
Mitochondria 0.0081 0.0009 0.0128 0.0143 0.0192 0.0127 0.0021 0.0005 0.0043 0.0715 0.0028 0.0031 0.0041 0.0003 0.0036 0.002 0.0003 0.0037
None 0.2604 0.2627 0.2264 0.2855 0.2261 0.2308 0.2746 0.1756 0.2258 0.2436 0.2657 0.2414 0.2899 0.2055 0.33 0.2633 0.4191 0.2738
Nuclear Periphery 0.0096 0.0015 0.0055 0.0108 0.0033 0.0056 0.007 0.0014 0.0103 0.0017 0.008 0.002 0.0031 0.0012 0.0042 0.0005 0.0012 0.0011
Nucleolus 0.0016 0.0008 0.0022 0.0018 0.0008 0.0016 0.0006 0.0001 0.0005 0.0002 0.0014 0.0001 0.0008 0.0001 0.0003 0.0008 0.0001 0.0004
Nucleus 0.012 0.0092 0.0232 0.0268 0.0068 0.0143 0.0044 0.0064 0.0135 0.0045 0.0087 0.0069 0.0047 0.0043 0.0113 0.0038 0.0032 0.0115
Peroxisomes 0.0102 0.0006 0.0109 0.0194 0.0295 0.0035 0.0112 0.0002 0.0149 0.011 0.0022 0.0092 0.0085 0.0012 0.0072 0.0389 0.001 0.006
Punctate Nuclear 0.0121 0.0109 0.0189 0.0458 0.0115 0.0221 0.0105 0.0027 0.0039 0.0029 0.0054 0.0038 0.005 0.0018 0.0031 0.0027 0.0037 0.0054
Vacuole 0.0027 0.0017 0.0037 0.0044 0.0019 0.0024 0.0012 0.001 0.0063 0.0005 0.0019 0.0008 0.0031 0.001 0.0011 0.0473 0.0005 0.0032
Vacuole Periphery 0.0013 0.0002 0.001 0.0016 0.0009 0.0009 0.0004 0.0002 0.0017 0.0003 0.0006 0.0003 0.0007 0.0002 0.0006 0.0014 0.0001 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.9516 7.9091 11.7378 12.0933 9.013 10.1058 11.1385 10.7947 13.6844 12.2969
Translational Efficiency 1.3611 1.1498 0.8906 0.8671 0.8784 1.656 0.9603 1.1831 1.1778 0.9767

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
528 1384 2308 1893 845 880 1993 1773 1373 2264 4301 3666

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 601.43 825.64 882.46 974.78 707.11 896.68 847.50 961.37 666.47 853.25 866.26 968.29
Standard Deviation 108.94 116.34 120.01 145.70 96.72 92.67 109.87 140.80 113.86 113.19 116.73 143.51
Intensity Change Log 2 — 0.457116 0.553134 0.696680 — 0.342659 0.261279 0.443157 — 0.396395 0.402781 0.565233

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000116 0.000236 0.000280 0.001054 0.000119 0.000185 0.000117 0.000737 0.000118 0.000216 0.000204 0.000901
Bud Neck 0.007768 0.012551 0.004869 0.007439 0.002191 0.008455 0.003495 0.006656 0.004335 0.010959 0.004232 0.007060
Bud Site 0.005398 0.013344 0.011818 0.023681 0.002279 0.006640 0.003647 0.030030 0.003478 0.010738 0.008032 0.026752
Cell Periphery 0.000228 0.000078 0.000091 0.000581 0.000148 0.000129 0.000141 0.000107 0.000179 0.000098 0.000114 0.000352
Cytoplasm 0.503642* 0.622505* 0.559123* 0.543585* 0.596126* 0.605444* 0.574828* 0.623124* 0.560560* 0.615873* 0.566401* 0.582053*
Cytoplasmic Foci 0.182160* 0.142463 0.006648 0.012734 0.152610 0.144219 0.015832 0.010930 0.163974 0.143146 0.010904 0.011861
Eisosomes 0.000360 0.000043 0.000016 0.000030 0.000080 0.000084 0.000037 0.000020 0.000188 0.000059 0.000026 0.000025
Endoplasmic Reticulum 0.001065 0.000743 0.001138 0.002017 0.001467 0.000511 0.000810 0.000981 0.001313 0.000653 0.000986 0.001516
Endosome 0.014422 0.011105 0.001292 0.007606 0.006910 0.008015 0.001712 0.005016 0.009799 0.009904 0.001486 0.006353
Golgi 0.001474 0.003110 0.000084 0.005406 0.000923 0.001939 0.000564 0.003968 0.001135 0.002655 0.000306 0.004710
Lipid Particles 0.010082 0.001771 0.000448 0.000836 0.005702 0.003152 0.000829 0.000537 0.007386 0.002308 0.000625 0.000691
Mitochondria 0.001825 0.003304 0.000758 0.002358 0.001912 0.003542 0.000475 0.002890 0.001878 0.003396 0.000627 0.002615
Mitotic Spindle 0.001524 0.003465 0.009415 0.086815 0.000233 0.003082 0.003371 0.029221 0.000729 0.003316 0.006614 0.058960
None 0.024185 0.003161 0.007279 0.010338 0.004377 0.003017 0.008642 0.007674 0.011995 0.003105 0.007911 0.009050
Nuclear Periphery 0.000639 0.000255 0.001283 0.002427 0.000584 0.000325 0.000717 0.000846 0.000605 0.000283 0.001021 0.001662
Nuclear Periphery Foci 0.001481 0.000817 0.000478 0.001898 0.002386 0.000508 0.000411 0.000996 0.002038 0.000697 0.000447 0.001462
Nucleolus 0.005321 0.000934 0.000238 0.000932 0.001589 0.000547 0.000421 0.000213 0.003024 0.000784 0.000323 0.000584
Nucleus 0.175047* 0.101197 0.349648* 0.166668* 0.162247* 0.157421* 0.319466* 0.208714* 0.167169* 0.123051 0.335662* 0.187002*
Peroxisomes 0.002947 0.003663 0.000548 0.000741 0.001429 0.003214 0.000349 0.001433 0.002013 0.003489 0.000456 0.001076
Vacuole 0.059229 0.073658 0.043949 0.116768 0.055979 0.048856 0.064008 0.063969 0.057229 0.064018 0.053244 0.091232
Vacuole Periphery 0.001088 0.001597 0.000596 0.006087 0.000710 0.000714 0.000130 0.001940 0.000855 0.001254 0.000380 0.004081

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.43 -1.49 -3.00 -1.96 -1.62 -0.89 -0.62 -4.71 -3.02 -4.34 -2.72 -1.59 -4.98 -3.40 -3.37
Bud Neck -2.72 1.98 1.78 6.08 -0.55 -4.35 -3.24 -5.72 2.05 -1.98 -6.46 -0.25 -1.66 5.97 -1.73
Bud Site -4.40 -2.09 -3.66 1.00 -1.56 -3.88 -2.20 -7.58 -4.12 -6.36 -7.06 -3.55 -8.71 -1.79 -5.40
Cell Periphery 6.59 6.24 0.51 -1.10 -1.02 1.72 2.66 5.45 3.54 3.43 7.57 7.24 0.82 -0.89 -0.78
Cytoplasm -8.58 2.42 5.01 19.51 4.10 -0.96 7.25 9.51 10.64 2.64 -6.29 8.12 11.48 21.42 4.59
Cytoplasmic Foci 4.81 23.64 23.51 29.05 -1.60 1.08 26.51 27.74 25.42 6.26 3.94 35.72 36.25 38.42 3.92
Eisosomes 2.98 3.18 3.09 4.41 -5.84 -0.53 10.19 13.68 10.74 6.92 3.15 3.86 3.88 11.31 0.62
Endoplasmic Reticulum 1.79 -5.41 -4.46 -9.29 0.96 4.36 0.94 0.29 -9.62 -1.45 4.33 -2.18 -2.08 -12.70 0.11
Endosome 1.93 7.98 7.23 8.85 -3.32 -1.02 10.56 8.41 6.57 -0.64 0.17 11.85 10.40 10.99 -2.60
Golgi -2.02 2.48 0.47 2.96 -3.11 -1.34 1.14 -0.41 0.95 -1.30 -2.90 2.78 -0.05 2.86 -2.85
Lipid Particles 6.84 7.87 7.49 3.41 -9.16 3.34 7.69 7.63 4.65 -0.62 8.00 11.01 10.56 5.79 -4.48
Mitochondria -1.75 3.80 2.31 2.75 -3.11 -1.01 1.99 0.82 1.54 -3.56 -1.73 2.77 1.42 2.91 -4.61
Mitotic Spindle -1.40 -4.76 -10.94 -10.63 -8.28 -2.86 -4.80 -8.33 -6.35 -5.40 -3.32 -7.16 -13.92 -12.31 -9.78
None 5.13 4.34 3.85 -7.57 -2.37 1.31 -2.85 -4.73 -6.31 -1.15 5.26 3.06 2.22 -10.44 -2.59
Nuclear Periphery 4.07 -12.64 -8.93 -11.58 -2.90 1.96 -3.78 -6.81 -13.23 -6.19 3.69 -10.45 -10.83 -16.02 -4.66
Nuclear Periphery Foci 2.63 4.50 1.14 -2.38 -7.10 2.69 2.63 1.69 -5.19 -5.82 2.96 3.35 1.85 -4.39 -9.05
Nucleolus 2.65 3.06 2.92 3.11 -2.54 2.88 3.28 3.70 3.99 4.09 3.23 3.92 3.82 4.51 -1.14
Nucleus 7.74 -22.52 -7.40 -24.65 21.42 0.74 -22.77 -15.90 -17.12 7.78 7.81 -33.65 -16.42 -30.65 21.06
Peroxisomes -1.26 6.28 7.60 10.43 0.41 -1.76 3.58 2.88 2.81 -0.92 -2.98 6.50 6.97 7.33 -0.20
Vacuole -3.32 -3.46 -23.49 -23.00 -23.29 1.97 -8.85 -16.82 -19.03 -9.12 -2.45 -8.67 -29.21 -28.56 -23.40
Vacuole Periphery -1.30 2.58 -0.63 0.48 -2.47 -0.04 5.36 0.55 0.52 -2.43 -1.71 4.32 -0.54 0.91 -3.22
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
Localization
Cell Percentages cytoplasm (68%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YOR385W

YOR385W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor385w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available