Standard name
Human Ortholog
Description Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 – 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.93 0.94 0.92 0.62 0.76 0.83 0.8 – 0.74 0.71 0.66 0.74 0.88 0.9 0.96 0.96 0.94 0.95 0.8 0.8 0.75 0.57 0.44 0.47
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0.18 0.11 0 0.07 – 0 0 0 0 0.09 0.09 0.05 0 0 0 0.08 0.08 0.07 0.23 0.32 0.24
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0.08 – 0.35 0.31 0.39 0.33 0 0 0 0 0 0 0 0 0.07 0.07 0.07 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.21 0.11 0 0.06 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 2 3 3 1 0 – 2 3 0 0 1 3 0 0 1 1 0 1 2 1 3 5
Bud 0 0 1 1 2 8 3 – 11 10 8 5 1 3 1 0 0 0 0 1 0 0 2 8
Bud Neck 0 0 4 3 2 1 1 – 1 0 0 1 0 0 0 0 0 0 2 2 2 1 7 3
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 1 0 5 – 0 1 0 3 4 2 0 2 5 5 0 0 0 0 0 0
Cytoplasm 188 182 140 68 164 120 215 – 121 206 96 175 235 333 388 129 91 105 176 171 121 172 178 177
Endoplasmic Reticulum 4 3 6 4 0 5 0 – 0 0 0 3 13 4 1 8 5 6 3 2 3 9 11 11
Endosome 5 5 0 20 23 2 19 – 3 8 6 8 23 33 20 2 1 0 18 16 11 70 127 92
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 4
Mitochondria 2 2 3 2 6 25 21 – 57 89 57 77 0 0 0 3 2 1 5 6 11 20 26 24
Nucleus 0 2 1 1 1 2 3 – 2 1 4 0 0 0 0 0 1 1 0 0 0 0 1 0
Nuclear Periphery 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 1 0 4 – 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 23 24 2 17 – 4 19 4 5 4 4 0 1 0 0 1 1 1 4 8 10
Vac/Vac Membrane 5 0 0 0 7 0 13 – 1 4 1 4 2 4 3 2 1 1 8 7 2 13 30 31
Unique Cell Count 202 194 153 109 216 144 268 164 290 146 235 268 369 405 134 97 110 221 216 162 302 405 377
Labelled Cell Count 207 195 157 126 234 166 301 202 342 176 283 283 386 414 148 107 120 221 216 162 302 405 377


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.5 5.5 5.5 6.2 4.0 6.6 – 4.4 4.6 4.4 4.5 5.8 6.1 6.2 7.1 6.9 6.5 5.2 5.5 5.8
Std Deviation (1e-4) 0.7 1.2 0.7 1.6 1.8 1.0 5.8 – 1.2 1.7 1.2 0.7 0.8 1.1 1.3 2.2 1.5 1.1 1.1 1.9 2.1
Intensity Change (Log2) – – – 0.01 0.18 -0.46 0.28 – -0.3 -0.25 -0.3 -0.28 0.09 0.15 0.18 0.37 0.33 0.25 -0.06 0.01 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -5.7 -3.9 -2.1 -3.1 – -4.1 -5.0 -5.5 -4.2 -1.2 -0.4 2.0 1.7 0.7 1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0.4 0 0 0.8 0 0.6
Endosome 5.5 4.2 0 3.4 – 0 0 0 0 3.7 3.8 2.8 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.5 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 5.9 4.3 0 3.2 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2 -0.7089 -0.9595 -1.0744 -1.4583 -0.9548 1.2393 1.5788 1.1836 1.0639 0.8958 1.3296 2.8598 3.2673 3.1066 2.8386 3.1511 3.1732
Actin 0.0251 0.0034 0.0108 0.0429 0.0163 0.0033 0.0378 0.0054 0.0066 0.0135 0.0001 0.0176 0.0109 0.0001 0.0029 0.0024 0.0064 0.0084
Bud 0.0008 0.0003 0.002 0.0066 0.0004 0.0019 0.0055 0.0157 0.0095 0.0062 0.0002 0.0079 0.0019 0.0034 0.0014 0.0064 0.0043 0.0009
Bud Neck 0.0006 0.0001 0.0008 0.0029 0.0007 0.0006 0.0023 0.0006 0.0004 0.0008 0.0001 0.0007 0.0004 0.0002 0.0002 0.0009 0.0043 0.0013
Bud Periphery 0.0014 0.0003 0.0038 0.0156 0.0006 0.0023 0.0047 0.0099 0.0096 0.0226 0.0002 0.0047 0.0047 0.0037 0.001 0.0053 0.008 0.0018
Bud Site 0.0031 0.0007 0.0058 0.0024 0.0006 0.0013 0.0069 0.0402 0.0039 0.0137 0.0001 0.0004 0.0051 0.0015 0.0023 0.0043 0.0113 0.0003
Cell Periphery 0.0003 0.0002 0.0006 0.0005 0.0002 0.0001 0.0006 0.0016 0.0004 0.0012 0 0.0001 0.0004 0.0004 0.0002 0.0002 0.0008 0.0001
Cytoplasm 0.3673 0.5287 0.4152 0.3238 0.3789 0.5235 0.3266 0.4964 0.5035 0.6318 0.5147 0.502 0.3382 0.4497 0.4054 0.4454 0.3927 0.4851
Cytoplasmic Foci 0.0719 0.0336 0.0422 0.0524 0.0926 0.051 0.0652 0.0263 0.019 0.0489 0.0151 0.0325 0.0388 0.0258 0.0236 0.0265 0.0436 0.0367
Eisosomes 0.0006 0.0002 0.0011 0.0002 0.0002 0.0001 0.0003 0.0004 0.0001 0.0001 0 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0071 0.0059 0.0049 0.0026 0.0091 0.0031 0.005 0.0022 0.0034 0.0032 0.0006 0.0018 0.0031 0.0018 0.0027 0.0021 0.0014 0.0012
Endosome 0.0695 0.0268 0.0497 0.0858 0.0903 0.0308 0.0675 0.0151 0.0228 0.0223 0.0348 0.0197 0.018 0.0166 0.0246 0.0135 0.0345 0.0184
Golgi 0.0169 0.0023 0.0071 0.0234 0.0154 0.0049 0.0236 0.0029 0.0057 0.0039 0.0092 0.0051 0.0016 0.0006 0.001 0.002 0.0011 0.001
Lipid Particles 0.0175 0.0048 0.0079 0.0019 0.0152 0.0062 0.0123 0.0007 0.0023 0.0018 0.0005 0.0021 0.0016 0.0003 0.0006 0.0021 0.0007 0.0003
Mitochondria 0.0128 0.0048 0.0095 0.006 0.0073 0.0022 0.0183 0.0023 0.0034 0.0055 0.0264 0.0027 0.0022 0.0004 0.0009 0.0013 0.0012 0.0006
None 0.372 0.3705 0.4107 0.4067 0.3404 0.3562 0.3624 0.3641 0.3984 0.2012 0.3913 0.3926 0.5472 0.4876 0.5242 0.4597 0.4553 0.438
Nuclear Periphery 0.0039 0.0018 0.0033 0.0017 0.0124 0.002 0.01 0.0007 0.0012 0.0019 0.0003 0.0012 0.0017 0.0004 0.0008 0.0027 0.0019 0.0003
Nucleolus 0.0005 0.0003 0.0007 0.0002 0.0004 0.0002 0.0025 0.0008 0.0002 0.0003 0 0.0002 0.004 0.0003 0.0002 0.0011 0.0023 0.0001
Nucleus 0.0016 0.0014 0.0018 0.0014 0.0021 0.0009 0.0121 0.0032 0.0013 0.0022 0.0007 0.003 0.0023 0.0012 0.0013 0.0037 0.0124 0.0006
Peroxisomes 0.0166 0.0028 0.0064 0.0054 0.0047 0.0019 0.0233 0.0028 0.0026 0.0108 0.001 0.0011 0.0082 0.0001 0.0003 0.0024 0.0019 0.002
Punctate Nuclear 0.0033 0.0008 0.0022 0.002 0.005 0.0028 0.0059 0.0049 0.0006 0.0018 0.0002 0.0018 0.0055 0.0006 0.0012 0.0141 0.0075 0.0006
Vacuole 0.0058 0.009 0.0114 0.0131 0.0047 0.0038 0.0057 0.0036 0.0043 0.0055 0.0031 0.002 0.0034 0.0048 0.0045 0.0032 0.0074 0.0019
Vacuole Periphery 0.0013 0.0013 0.0017 0.0025 0.0026 0.0009 0.0016 0.0005 0.0009 0.0009 0.0013 0.0005 0.0006 0.0004 0.0007 0.0006 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.0731 7.0255 11.469 13.0622 17.3222 6.2837 14.5621 17.2597 14.5151 16.713
Translational Efficiency 1.2527 1.4231 0.6748 0.6197 0.4261 1.7819 0.7371 0.5386 0.822 0.84

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2288 693 550 1606 1908 2401 1476 1137 4196 3094 2026 2743

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 710.00 706.93 1008.67 916.61 715.78 795.89 871.66 938.33 712.63 775.96 908.85 925.61
Standard Deviation 80.49 96.89 107.40 121.58 80.84 96.63 112.02 132.69 80.70 103.56 126.43 126.75
Intensity Change Log 2 — -0.006252 0.506563 0.368489 — 0.153053 0.284249 0.390579 — 0.075921 0.399234 0.379621

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000329 0.000335 0.000300 0.001854 0.000231 0.000483 0.000178 0.001520 0.000285 0.000450 0.000211 0.001715
Bud Neck 0.027702 0.021548 0.002652 0.008876 0.020686 0.026313 0.002099 0.008333 0.024512 0.025245 0.002249 0.008651
Bud Site 0.011266 0.020858 0.013547 0.054820 0.007925 0.020627 0.004607 0.067632 0.009747 0.020678 0.007034 0.060130
Cell Periphery 0.000121 0.000109 0.000137 0.000088 0.000092 0.000127 0.000073 0.000141 0.000108 0.000123 0.000090 0.000110
Cytoplasm 0.377152* 0.360532* 0.499290* 0.506905* 0.339237* 0.431917* 0.667101* 0.438330* 0.359911* 0.415928* 0.621545* 0.478480*
Cytoplasmic Foci 0.329725* 0.325206* 0.043820 0.049515 0.344113* 0.245076* 0.047438 0.065655 0.336267* 0.263024* 0.046456 0.056205
Eisosomes 0.000345 0.000127 0.000052 0.000037 0.000176 0.000133 0.000030 0.000043 0.000268 0.000132 0.000036 0.000039
Endoplasmic Reticulum 0.002455 0.001327 0.004225 0.000942 0.002383 0.001102 0.001294 0.001942 0.002423 0.001152 0.002090 0.001357
Endosome 0.056782 0.069848 0.012568 0.019873 0.089371 0.058280 0.007655 0.040578 0.071601 0.060871 0.008989 0.028456
Golgi 0.017343 0.008821 0.000158 0.004062 0.009548 0.007190 0.000151 0.010847 0.013798 0.007555 0.000153 0.006874
Lipid Particles 0.016486 0.006698 0.003258 0.001580 0.020606 0.008709 0.001608 0.001536 0.018359 0.008258 0.002056 0.001562
Mitochondria 0.006234 0.004827 0.000217 0.003789 0.004876 0.003217 0.000174 0.004468 0.005617 0.003577 0.000186 0.004070
Mitotic Spindle 0.001734 0.001536 0.009395 0.059741 0.004225 0.004028 0.006640 0.053581 0.002867 0.003470 0.007388 0.057187
None 0.001282 0.003024 0.002205 0.002385 0.000591 0.001220 0.004233 0.001468 0.000968 0.001624 0.003683 0.002005
Nuclear Periphery 0.000476 0.000232 0.001965 0.001027 0.000645 0.000231 0.000833 0.000850 0.000553 0.000231 0.001140 0.000953
Nuclear Periphery Foci 0.000319 0.000630 0.008910 0.001552 0.000801 0.000346 0.004448 0.002281 0.000538 0.000410 0.005660 0.001854
Nucleolus 0.000693 0.002323 0.000333 0.000734 0.000924 0.000828 0.000481 0.000478 0.000798 0.001163 0.000441 0.000628
Nucleus 0.035350 0.027524 0.159292* 0.050339 0.031851 0.038755 0.117227 0.034817 0.033759 0.036239 0.128647 0.043905
Peroxisomes 0.008442 0.009251 0.000391 0.007587 0.009042 0.006642 0.001346 0.016518 0.008715 0.007227 0.001087 0.011289
Vacuole 0.102936 0.129037 0.237000* 0.222421* 0.110111 0.141235 0.132211 0.241001* 0.106199 0.138503 0.160659 0.230122*
Vacuole Periphery 0.002828 0.006208 0.000285 0.001875 0.002567 0.003543 0.000173 0.007982 0.002709 0.004140 0.000203 0.004406

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.06 -0.85 -3.72 -3.54 -3.53 -2.01 0.43 -4.43 -3.02 -4.51 -1.68 1.02 -5.79 -3.86 -6.02
Bud Neck 2.61 19.48 15.99 6.46 -6.40 -3.48 15.83 10.29 13.56 -7.21 -0.54 25.29 18.94 15.80 -10.08
Bud Site -5.85 0.16 -5.61 -1.36 -4.02 -7.42 2.85 -9.46 -5.26 -10.38 -8.06 3.13 -10.74 -4.47 -11.72
Cell Periphery 1.45 -1.23 10.50 5.45 7.39 -1.52 2.11 -0.17 1.44 -2.54 -0.88 3.37 4.72 2.77 1.87
Cytoplasm 1.40 -3.43 -6.25 -5.90 -1.08 -10.89 -26.14 -5.02 3.63 16.97 -8.54 -24.17 -8.23 -0.28 14.56
Cytoplasmic Foci 0.67 57.21 58.42 32.40 -1.17 14.88 53.91 51.18 38.85 -2.09 14.25 76.77 77.88 52.46 -1.02
Eisosomes 9.39 16.67 17.91 5.49 5.52 3.38 12.65 11.70 13.16 -3.91 11.15 21.21 21.19 14.41 -0.06
Endoplasmic Reticulum 2.38 -5.03 3.87 0.99 11.96 2.84 1.84 1.94 -1.24 0.58 4.23 -0.17 4.13 -0.19 8.55
Endosome -2.94 18.33 19.92 15.34 -0.32 7.93 24.19 17.31 12.96 -6.70 4.20 31.96 25.89 22.24 -6.07
Golgi 7.34 20.79 15.73 7.25 -1.77 3.11 15.34 4.46 2.74 -3.43 9.46 25.58 14.58 7.39 -3.79
Lipid Particles 9.35 16.31 18.65 6.79 4.11 8.42 14.24 14.13 13.60 -1.16 11.95 21.86 22.32 15.25 1.47
Mitochondria 1.26 14.28 7.29 2.82 -2.28 2.14 6.24 2.92 1.85 -2.31 4.11 13.20 6.86 3.89 -3.26
Mitotic Spindle 0.25 -2.62 -10.57 -10.57 -6.92 0.06 -2.69 -9.38 -9.55 -8.03 -0.85 -4.38 -14.07 -13.76 -10.89
None -1.57 -1.72 -5.25 0.27 -3.41 -2.84 -11.49 -5.69 -1.93 6.35 -2.07 -9.77 -7.69 -2.20 4.24
Nuclear Periphery 0.88 -6.72 -4.82 -17.02 4.07 1.75 -2.72 -3.42 -14.21 -1.95 1.73 -5.66 -5.98 -22.56 -0.84
Nuclear Periphery Foci -3.67 -10.78 -10.16 -7.31 8.75 1.89 -7.78 -4.46 -8.95 5.23 1.10 -12.98 -8.87 -12.70 9.46
Nucleolus -2.18 3.00 -1.47 1.63 -2.88 0.91 1.91 1.48 1.36 -0.83 -1.58 2.56 -0.39 1.14 -2.47
Nucleus 2.56 -17.21 -15.82 -14.00 11.36 -3.73 -25.84 -7.11 -4.34 18.82 -1.69 -30.76 -17.02 -14.20 19.84
Peroxisomes -0.78 16.91 4.34 4.14 -5.66 3.00 10.82 0.09 -1.45 -5.06 2.74 17.60 2.71 0.99 -6.92
Vacuole -4.51 -18.18 -30.98 -22.58 -2.66 -6.99 -12.78 -27.09 -23.35 -16.34 -9.40 -21.04 -41.10 -33.39 -16.74
Vacuole Periphery -5.64 20.78 6.52 8.42 -1.18 -3.77 16.24 -0.47 0.77 -3.67 -6.14 26.56 3.00 6.04 -3.85
Standard name
Human Ortholog
Description Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Snf8

Snf8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snf8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available