Standard name | |
---|---|
Human Ortholog | |
Description | Eisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 58 | 80 | 31 | 25 | 27 | 44 | 38 | 87 | 61 | 84 | 65 | 71 | 14 | 10 | 26 | 51 | 35 | 60 | 86 | 60 | 37 | 35 | 30 | 15 |
Bud | 17 | 18 | 6 | 2 | 9 | 10 | 17 | 27 | 15 | 23 | 11 | 15 | 4 | 0 | 3 | 3 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 8 | 4 | 5 | 5 | 9 | 11 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 40 | 86 | 43 | 38 | 47 | 58 | 59 | 58 | 54 | 72 | 51 | 53 | 34 | 90 | 129 | 168 | 110 | 134 | 82 | 92 | 83 | 59 | 46 | 26 |
Cytoplasm | 77 | 29 | 82 | 74 | 125 | 132 | 119 | 87 | 51 | 45 | 25 | 15 | 153 | 284 | 265 | 108 | 70 | 102 | 9 | 1 | 6 | 43 | 182 | 219 |
Endoplasmic Reticulum | 6 | 11 | 6 | 0 | 4 | 17 | 9 | 20 | 37 | 22 | 14 | 25 | 6 | 2 | 1 | 10 | 9 | 11 | 5 | 3 | 2 | 3 | 6 | 7 |
Endosome | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 6 | 3 | 3 | 3 | 2 | 1 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 25 | 18 | 2 | 6 | 13 | 71 | 117 | 112 | 129 | 142 | 116 | 101 | 1 | 0 | 0 | 4 | 10 | 6 | 10 | 6 | 17 | 17 | 12 | 9 |
Nucleus | 0 | 0 | 2 | 0 | 1 | 2 | 3 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 1 | 1 | 0 | 1 | 9 | 6 | 11 | 3 | 5 | 2 | 1 | 0 | 1 | 5 | 0 | 4 | 3 | 3 | 1 | 6 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 7 | 4 | 2 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 4 | 9 | 3 | 3 | 7 | 2 | 0 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 0 | 3 | 0 | 1 | 0 | 0 | 1 | 3 | 4 | 5 | 1 | 1 | 2 | 1 | 1 | 3 |
Unique Cell Count | 171 | 150 | 134 | 114 | 178 | 239 | 269 | 284 | 230 | 234 | 177 | 180 | 178 | 310 | 310 | 271 | 176 | 235 | 223 | 178 | 170 | 173 | 302 | 303 |
Labelled Cell Count | 228 | 243 | 182 | 150 | 230 | 349 | 374 | 412 | 352 | 400 | 285 | 283 | 212 | 393 | 440 | 356 | 249 | 333 | 223 | 178 | 170 | 173 | 302 | 303 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 6.8 | 5.7 | 6.0 | 6.2 | 5.6 | 5.7 | 6.1 | 6.2 | 6.8 | 7.1 | 7.9 | 6.1 | 6.7 | 6.7 | 8.7 | 8.3 | 8.3 | 5.8 | 6.4 | 6.5 |
Std Deviation (1e-4) | 0.5 | 1.0 | 0.9 | 1.5 | 1.4 | 1.0 | 1.2 | 1.0 | 1.2 | 1.2 | 1.5 | 1.7 | 0.8 | 1.1 | 1.0 | 1.3 | 1.5 | 1.6 | 1.0 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.12 | -0.02 | -0.01 | 0.1 | 0.12 | 0.25 | 0.32 | 0.46 | 0.09 | 0.23 | 0.23 | 0.61 | 0.55 | 0.54 | 0.02 | 0.16 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | -1.8 | -1.1 | -2.3 | 1.6 | 0.7 | 2.5 | 2.6 | 3.1 | -3.8 | -6.7 | -4.3 | -1.0 | -0.7 | 0.5 |
Bud | 0 | 0.2 | -0.1 | 0.8 | 1.8 | 0.8 | 1.8 | 0.7 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.2 | -1.1 | -1.6 | -2.2 | -2.6 | -1.8 | -0.3 | -0.6 | -0.5 | -2.6 | -0.6 | 1.9 | 5.7 | 5.3 | 4.6 |
Cytoplasm | 0.6 | 1.7 | -1.1 | -3.2 | -5.9 | -7.5 | -8.1 | -8.7 | -10.0 | 5.0 | 7.7 | 5.7 | -4.0 | -3.7 | -3.3 |
Endoplasmic Reticulum | 0 | 0 | 1.0 | 0 | 1.0 | 3.3 | 1.7 | 1.2 | 2.8 | -0.5 | 0 | 0 | -0.4 | 0.3 | 0.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.4 | 6.6 | 8.7 | 8.1 | 10.5 | 11.2 | 11.5 | 10.2 | 0 | 0 | 0 | 0 | 1.9 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.4639 | 9.8806 | 8.649 | 7.5654 | 7.9228 | 8.4962 | 7.1694 | 8.6867 | 8.403 | 8.7392 | 7.2384 | 8.1693 | 11.0785 | 14.1186 | 12.0177 | 11.911 | 11.1554 | 11.3963 |
Actin | 0.0771 | 0.0973 | 0.0643 | 0.0674 | 0.1074 | 0.0244 | 0.1134 | 0.164 | 0.1522 | 0.1354 | 0.1488 | 0.0575 | 0.063 | 0.0582 | 0.0364 | 0.1059 | 0.0368 | 0.0277 |
Bud | 0.0019 | 0.0002 | 0.0002 | 0.0062 | 0.0002 | 0.0003 | 0.0002 | 0.0008 | 0.0004 | 0.0018 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0004 |
Bud Neck | 0.0012 | 0.0025 | 0.004 | 0.0017 | 0.0039 | 0.0015 | 0.0044 | 0.005 | 0.0092 | 0.015 | 0.0036 | 0.0022 | 0.0014 | 0.0026 | 0.0037 | 0.0083 | 0.0022 | 0.0017 |
Bud Periphery | 0.0041 | 0.0012 | 0.0018 | 0.0048 | 0.0021 | 0.005 | 0.0015 | 0.0028 | 0.0023 | 0.0067 | 0.0036 | 0.0034 | 0.0026 | 0.0008 | 0.001 | 0.0041 | 0.0016 | 0.0016 |
Bud Site | 0.0052 | 0.0184 | 0.0071 | 0.0034 | 0.0022 | 0.0008 | 0.0073 | 0.0176 | 0.0168 | 0.0159 | 0.0021 | 0.0009 | 0.0051 | 0.0186 | 0.005 | 0.008 | 0.0023 | 0.0005 |
Cell Periphery | 0.0866 | 0.0678 | 0.1244 | 0.0906 | 0.1735 | 0.1328 | 0.1079 | 0.0857 | 0.1049 | 0.096 | 0.2222 | 0.1795 | 0.0639 | 0.0619 | 0.0829 | 0.1086 | 0.0979 | 0.0929 |
Cytoplasm | 0.0091 | 0.0183 | 0.0153 | 0.0263 | 0.0278 | 0.0116 | 0.0095 | 0.0161 | 0.03 | 0.0948 | 0.0204 | 0.0077 | 0.006 | 0.0038 | 0.0056 | 0.0162 | 0.0054 | 0.0059 |
Cytoplasmic Foci | 0.0052 | 0.0021 | 0.0005 | 0.0006 | 0.0022 | 0.0006 | 0.0016 | 0.001 | 0.0007 | 0.0013 | 0.0011 | 0.0003 | 0.0008 | 0.0003 | 0.0002 | 0.0009 | 0.0002 | 0.0008 |
Eisosomes | 0.7792 | 0.7355 | 0.7619 | 0.7603 | 0.6324 | 0.7939 | 0.7387 | 0.6806 | 0.6714 | 0.6114 | 0.5828 | 0.7381 | 0.8449 | 0.8435 | 0.8595 | 0.7273 | 0.8417 | 0.8598 |
Endoplasmic Reticulum | 0.0047 | 0.0059 | 0.0052 | 0.0098 | 0.015 | 0.0058 | 0.0017 | 0.0025 | 0.0037 | 0.0033 | 0.0031 | 0.0044 | 0.0029 | 0.0027 | 0.0021 | 0.0093 | 0.0057 | 0.0031 |
Endosome | 0.001 | 0.0011 | 0.0001 | 0.0003 | 0.0009 | 0.0005 | 0.001 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 |
Golgi | 0.0112 | 0.045 | 0.0124 | 0.0222 | 0.0263 | 0.0175 | 0.0029 | 0.0198 | 0.0063 | 0.015 | 0.003 | 0.0026 | 0.0024 | 0.0022 | 0.0018 | 0.0049 | 0.0047 | 0.003 |
Lipid Particles | 0.0017 | 0.0009 | 0.0003 | 0.0007 | 0.0023 | 0.0004 | 0.0015 | 0.0003 | 0.0004 | 0.0008 | 0.001 | 0.0003 | 0.0008 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0004 |
Mitochondria | 0.0046 | 0.0023 | 0.0008 | 0.0023 | 0.0011 | 0.003 | 0.0008 | 0.0023 | 0.0005 | 0.0004 | 0.0029 | 0.0011 | 0.002 | 0.0042 | 0.0005 | 0.0032 | 0.0001 | 0.0005 |
None | 0.0042 | 0.0009 | 0.0007 | 0.0014 | 0.0015 | 0.0011 | 0.0043 | 0.0006 | 0.0008 | 0.0005 | 0.0015 | 0.0012 | 0.0017 | 0.0005 | 0.0005 | 0.0005 | 0.0005 | 0.0011 |
Nuclear Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 |
Nucleolus | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0005 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Peroxisomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0027 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0012 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0007 | 0.0001 | 0.0003 | 0.0011 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 175.2972 | 137.4774 | 234.7039 | 237.3447 | 137.3443 | 231.0922 | 185.6617 | 228.3119 | 332.1886 | 153.6888 |
Translational Efficiency | 1.8132 | 1.6878 | 1.5719 | 2.8551 | 2.077 | 1.8417 | 1.8188 | 2.4403 | 1.8824 | 2.0106 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2003 | 1426 | 2974 | 1035 | 1462 | 2227 | 382 | 1445 | 3465 | 3653 | 3356 | 2480 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 726.08 | 822.89 | 838.37 | 971.84 | 698.11 | 800.34 | 890.58 | 985.01 | 714.28 | 809.14 | 844.31 | 979.51 |
Standard Deviation | 80.85 | 108.85 | 107.45 | 147.49 | 85.43 | 96.42 | 138.01 | 138.79 | 83.96 | 102.04 | 112.58 | 142.63 |
Intensity Change Log 2 | — | 0.180571 | 0.207459 | 0.420590 | — | 0.197159 | 0.351291 | 0.496684 | — | 0.188726 | 0.279755 | 0.458392 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.064573 | 0.212778* | 0.253979* | 0.444443* | 0.110324* | 0.137272* | 0.256509* | 0.436167* | 0.083877 | 0.166747* | 0.254267* | 0.439621* |
Bud Neck | 0.009768 | 0.004743 | 0.025080 | 0.009729 | 0.007661 | 0.004440 | 0.009701 | 0.011271 | 0.008879 | 0.004558 | 0.023330 | 0.010628 |
Bud Site | 0.004825 | 0.015550 | 0.050161 | 0.059570 | 0.006160 | 0.007594 | 0.043563 | 0.064697 | 0.005388 | 0.010700 | 0.049410 | 0.062558 |
Cell Periphery | 0.052637 | 0.238595* | 0.063289 | 0.187277* | 0.081050 | 0.110626* | 0.060536 | 0.113087* | 0.064626 | 0.160581* | 0.062976 | 0.144049* |
Cytoplasm | 0.241980* | 0.049620 | 0.236565* | 0.045087 | 0.155451* | 0.064713 | 0.219352* | 0.103265* | 0.205471* | 0.058821 | 0.234605* | 0.078986 |
Cytoplasmic Foci | 0.047450 | 0.019741 | 0.011501 | 0.003831 | 0.044388 | 0.010545 | 0.062174 | 0.023016 | 0.046158 | 0.014135 | 0.017269 | 0.015009 |
Eisosomes | 0.375928* | 0.261238* | 0.239248* | 0.205601* | 0.387461* | 0.432519* | 0.099175 | 0.168020* | 0.380794* | 0.365657* | 0.223304* | 0.183704* |
Endoplasmic Reticulum | 0.002173 | 0.002864 | 0.005346 | 0.006273 | 0.002011 | 0.001588 | 0.011646 | 0.003453 | 0.002104 | 0.002086 | 0.006063 | 0.004630 |
Endosome | 0.002138 | 0.004440 | 0.000652 | 0.001893 | 0.002448 | 0.001735 | 0.003339 | 0.004649 | 0.002269 | 0.002791 | 0.000958 | 0.003499 |
Golgi | 0.028270 | 0.013063 | 0.003067 | 0.005928 | 0.024178 | 0.017935 | 0.014442 | 0.013164 | 0.026544 | 0.016033 | 0.004362 | 0.010144 |
Lipid Particles | 0.017092 | 0.011418 | 0.007431 | 0.004537 | 0.019831 | 0.016293 | 0.019013 | 0.013598 | 0.018248 | 0.014390 | 0.008749 | 0.009817 |
Mitochondria | 0.008755 | 0.005557 | 0.002184 | 0.001168 | 0.006890 | 0.004965 | 0.000392 | 0.001659 | 0.007968 | 0.005196 | 0.001980 | 0.001454 |
Mitotic Spindle | 0.000120 | 0.000145 | 0.000620 | 0.000976 | 0.000074 | 0.000153 | 0.003629 | 0.001862 | 0.000101 | 0.000149 | 0.000963 | 0.001492 |
None | 0.001589 | 0.000220 | 0.000793 | 0.000151 | 0.001043 | 0.000726 | 0.002070 | 0.000111 | 0.001358 | 0.000528 | 0.000938 | 0.000127 |
Nuclear Periphery | 0.000041 | 0.000037 | 0.000082 | 0.000050 | 0.000035 | 0.000022 | 0.000281 | 0.000054 | 0.000038 | 0.000028 | 0.000104 | 0.000052 |
Nuclear Periphery Foci | 0.000062 | 0.000050 | 0.000221 | 0.000167 | 0.000069 | 0.000042 | 0.001241 | 0.000397 | 0.000065 | 0.000045 | 0.000337 | 0.000301 |
Nucleolus | 0.000053 | 0.000027 | 0.000037 | 0.000007 | 0.000042 | 0.000010 | 0.000191 | 0.000019 | 0.000048 | 0.000017 | 0.000055 | 0.000014 |
Nucleus | 0.017980 | 0.003628 | 0.067029 | 0.005145 | 0.014127 | 0.001635 | 0.134494* | 0.003520 | 0.016354 | 0.002413 | 0.074708 | 0.004198 |
Peroxisomes | 0.121253* | 0.153069* | 0.014300 | 0.009059 | 0.133026* | 0.186573* | 0.017311 | 0.018813 | 0.126221* | 0.173494* | 0.014643 | 0.014742 |
Vacuole | 0.003115 | 0.002984 | 0.018337 | 0.008893 | 0.003581 | 0.000509 | 0.040871 | 0.018252 | 0.003312 | 0.001475 | 0.020902 | 0.014346 |
Vacuole Periphery | 0.000198 | 0.000233 | 0.000076 | 0.000213 | 0.000151 | 0.000107 | 0.000070 | 0.000925 | 0.000178 | 0.000156 | 0.000075 | 0.000628 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -18.48* | -30.93* | -34.40* | -17.51 | -16.20 | -4.18 | -6.76 | -33.46* | -31.13* | -11.77 | -17.23* | -28.90 | -49.66* | -34.89* | -23.10* |
Bud Neck | 5.78 | -11.63 | 0.12 | -5.04 | 11.08 | 4.05 | -1.01 | -3.67 | -8.80 | -2.34 | 7.50 | -12.74 | -2.42 | -9.95 | 11.04 |
Bud Site | -5.60 | -19.13 | -13.35 | -9.84 | -2.18 | -1.16 | -6.23 | -16.49 | -15.92 | -4.07 | -5.19 | -19.90 | -21.40 | -18.56 | -4.27 |
Cell Periphery | -24.37* | -3.04 | -17.34 | 5.31 | -16.22 | -5.75 | 3.88 | -4.96 | -0.24 | -7.35 | -22.55 | 1.34 | -16.34 | 3.25 | -17.24 |
Cytoplasm | 29.30 | 0.08 | 30.18 | 0.08 | 33.12 | 14.96 | -5.22 | 7.04 | -7.75 | 9.12 | 32.63 | -5.78 | 24.65 | -6.18 | 29.42 |
Cytoplasmic Foci | 8.88 | 14.85 | 18.24 | 7.50 | 9.46 | 11.77 | -2.32 | 6.18 | -6.23 | 6.01 | 15.93 | 14.61 | 14.44 | -0.79 | 1.24 |
Eisosomes | 12.53 | 17.48 | 18.02 | 6.21 | 3.72 | -4.65 | 26.84 | 23.16 | 33.02* | -8.33 | 2.18 | 24.43 | 30.00 | 29.09 | 5.74 |
Endoplasmic Reticulum | -1.41 | -5.68 | -3.29 | -2.69 | -0.68 | 1.27 | -4.29 | -3.00 | -4.29 | 3.54 | 0.07 | -7.42 | -4.27 | -4.38 | 1.85 |
Endosome | -4.08 | 5.79 | 0.49 | 3.80 | -2.77 | 1.73 | -1.65 | -3.57 | -4.93 | -2.05 | -1.59 | 6.20 | -3.03 | -1.64 | -6.89 |
Golgi | 8.09 | 19.74 | 11.30 | 3.49 | -1.82 | 3.30 | 4.67 | 5.29 | 2.54 | -0.22 | 7.97 | 22.40 | 11.40 | 4.24 | -5.31 |
Lipid Particles | 4.01 | 8.82 | 10.72 | 4.76 | 2.51 | 1.71 | 0.84 | 2.61 | 1.27 | 1.08 | 3.19 | 8.67 | 6.33 | 3.38 | -1.13 |
Mitochondria | 2.64 | 6.88 | 7.82 | 5.00 | 2.09 | 1.36 | 5.40 | 4.21 | 4.08 | -3.85 | 3.03 | 7.69 | 8.51 | 6.23 | 1.38 |
Mitotic Spindle | -0.57 | -2.00 | -1.28 | -1.25 | -0.66 | -1.49 | -1.88 | -3.42 | -3.26 | 1.12 | -1.25 | -2.77 | -3.12 | -3.02 | -0.84 |
None | 2.81 | 1.61 | 2.94 | 2.05 | 9.00 | 0.63 | -1.77 | 2.60 | 2.64 | 3.63 | 2.26 | 1.18 | 3.85 | 2.78 | 9.10 |
Nuclear Periphery | 1.28 | -8.15 | -2.54 | -2.79 | 1.31 | 4.43 | -9.52 | -0.86 | -1.44 | 8.10 | 5.25 | -12.18 | -1.84 | -2.57 | 4.62 |
Nuclear Periphery Foci | 1.46 | -5.52 | -4.17 | -4.46 | 1.31 | 3.65 | -7.83 | -3.29 | -3.55 | 4.93 | 3.81 | -8.75 | -4.06 | -4.37 | 0.75 |
Nucleolus | 3.31 | 1.62 | 6.17 | 4.18 | 7.04 | 3.96 | -2.97 | 2.65 | -2.30 | 3.30 | 5.77 | -1.58 | 5.95 | 0.65 | 5.39 |
Nucleus | 10.14 | -18.52 | 4.27 | -2.83 | 20.04 | 9.18 | -11.84 | 7.66 | -6.61 | 12.70 | 14.52 | -22.46 | 8.98 | -4.30 | 26.49 |
Peroxisomes | -4.75 | 28.91* | 30.10* | 25.37 | 4.46 | -7.98 | 22.89 | 23.07 | 33.89* | -1.53 | -10.15 | 37.46* | 36.75* | 42.35* | -0.47 |
Vacuole | 0.03 | -15.97 | -5.42 | -5.19 | 2.68 | 6.17 | -8.86 | -9.18 | -11.32 | 6.09 | 4.53 | -17.79 | -10.58 | -11.84 | 4.39 |
Vacuole Periphery | -0.69 | 3.79 | -0.15 | 0.23 | -1.53 | 1.00 | 2.09 | -2.26 | -2.39 | -2.49 | 0.71 | 3.49 | -2.21 | -2.31 | -2.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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