Standard name
Human Ortholog
Description Peripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.11 0.12 0.14 0.07 0.09 0.08 0.06 0.06 0.05 0.05 0.14 0.13 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.07 0.07 0.06 0 0 0 0 0.05 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0.1 0.18 0.11 0.21 0.19 0.13 0.08 0.07 0 0 0 0 0.23 0.26 0.31 0.14 0.16 0.1 0 0.15 0
Golgi 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.08 0.07 0.05
Mitochondria 0.83 0.61 0.28 0.55 0.43 0.68 0.7 0.68 0.84 0.85 0.92 0.91 0.17 0.07 0.12 0.62 0.59 0.69 0.69 0.55 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.1 0.08 0.08 0.05 0.05 0.05 0 0 0 0 0 0 0.11 0.14 0.18 0.09 0.05 0.05 0 0 0
SpindlePole 0.08 0.05 0.2 0.07 0.05 0.07 0.06 0.16 0.06 0.06 0 0.07 0.32 0.33 0.34 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.19 0.18 0 0.14 0.07 0.06 0 0.07 0.07 0 0 0.12 0.13 0.15 0 0 0 0 0 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 6 1 2 0 2 3 0 0 2 0 4 5 1 1 6 4 0 0 1
Bud 13 3 1 2 1 0 4 4 3 4 7 8 11 10 7 1 3 0 0 0 0
Bud Neck 0 0 2 0 0 0 1 1 1 1 0 1 2 0 2 0 2 1 0 0 0
Bud Site 3 0 0 0 0 0 0 1 2 1 1 0 3 5 2
Cell Periphery 1 12 2 0 3 0 4 0 1 0 0 2 9 11 3 0 0 0 0 0 0
Cytoplasm 10 11 7 5 12 7 15 12 8 9 12 12 26 24 26 1 0 0 0 0 0
Endoplasmic Reticulum 4 5 2 3 6 6 4 5 3 4 13 4 11 6 9 4 0 0 0 0 0
Endosome 27 40 7 9 17 12 13 11 2 1 1 1 43 48 55 39 41 14 0 1 1
Golgi 0 11 1 0 4 0 0 1 0 0 0 0 5 6 5 10 18 2 1 0 1
Mitochondria 228 133 17 23 38 64 111 108 113 126 228 206 33 14 22 179 150 100 10 7 21
Nucleus 0 1 0 0 0 2 5 5 3 4 2 4 2 5 6 1 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 1 1 3 0 3 0 1 0 0 0 2 0 2 0 0 1
Nucleolus 4 1 0 0 0 2 1 0 1 0 0 1 0 0 2 1 0 1 0 0 0
Peroxisomes 27 17 5 2 4 5 1 4 1 4 1 2 20 26 32 26 13 6 0 0 1
SpindlePole 21 12 12 3 4 7 10 26 8 9 5 17 61 62 60 5 7 2 0 0 0
Vac/Vac Membrane 23 41 11 0 12 7 9 6 9 10 11 9 23 25 26 6 7 3 0 0 4
Unique Cell Count 276 219 61 42 88 94 158 159 134 149 247 227 190 187 178 289 257 145 15 13 38
Labelled Cell Count 363 287 73 48 103 113 181 190 155 176 283 268 253 247 258 289 257 145 15 13 38


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.8 6.0 6.0 6.0 5.2 5.2 5.3 4.4 4.6 4.0 4.1 6.3 6.5 6.6
Std Deviation (1e-4) 0.7 1.1 0.9 1.8 1.4 1.6 1.4 1.4 1.7 1.3 0.8 1.0 1.4 1.1 1.6
Intensity Change (Log2) 0.0 0.01 -0.22 -0.2 -0.17 -0.45 -0.39 -0.58 -0.54 0.08 0.12 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0
Endosome 1.4 1.3 0.2 -0.7 0 0 0 1.9 2.3 3.0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 1.9 4.9 5.7 5.4 0 0 -1.8 -4.2 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0.5 1.2 1.8
SpindlePole -1.8 -2.9 -2.3 -2.9 -0.6 0 0 1.9 2.0 2.1
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.4 -0.9 -0.4 -0.9 0 0 0.4 0.3 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.7 -2.0 -2.8 0 -2.4 -2.5 -1.2 -0.9 -0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9943 1.1312 1.1445 0.9153 0.6758 1.0763 0.8714 0.5615 0.6968 0.7083 0.7142 0.635 0.2533 0.7121 0.5433 0.5316 0.7813 0.3237
Actin 0.0258 0.0128 0.015 0.0019 0.0091 0.0051 0.0675 0.0195 0.0271 0.0083 0.0134 0.012 0.0526 0.0312 0.0789 0.0153 0.0587 0.0612
Bud 0.0029 0.0086 0.0038 0.0006 0.0004 0.0012 0.0017 0.003 0.0015 0.0007 0.002 0.0006 0.0008 0.0015 0.0027 0.0004 0.0005 0.0004
Bud Neck 0.003 0.0024 0.0045 0.0003 0.0002 0.0033 0.0018 0.0009 0.0032 0.0007 0.001 0.0017 0.0083 0.0008 0.0043 0.0006 0.0008 0.0434
Bud Periphery 0.0025 0.0037 0.0057 0.0012 0.001 0.0016 0.0027 0.0069 0.0039 0.0015 0.0069 0.0023 0.0015 0.002 0.0042 0.0016 0.0009 0.0006
Bud Site 0.0084 0.0336 0.0162 0.0016 0.0007 0.0011 0.0129 0.0148 0.0053 0.0015 0.0062 0.0009 0.0241 0.0189 0.011 0.0007 0.0015 0.006
Cell Periphery 0.0007 0.0008 0.0044 0.0003 0.0002 0.0004 0.001 0.0008 0.0032 0.0003 0.0017 0.0005 0.0008 0.0011 0.0023 0.0002 0.0001 0.0003
Cytoplasm 0.0066 0.0138 0.0127 0.0026 0.0008 0.0105 0.017 0.008 0.0141 0.0053 0.0073 0.0044 0.0051 0.0499 0.0226 0.0003 0.0275 0.0074
Cytoplasmic Foci 0.073 0.0642 0.0408 0.0717 0.0209 0.0504 0.0656 0.0833 0.0386 0.0338 0.0365 0.0573 0.0638 0.0897 0.0875 0.0484 0.0531 0.1181
Eisosomes 0.0007 0.0004 0.0022 0.0002 0.0005 0.0003 0.001 0.0007 0.0019 0.0003 0.0007 0.0003 0.0011 0.001 0.0039 0.0003 0.0004 0.0003
Endoplasmic Reticulum 0.0036 0.0008 0.0012 0.0011 0.0033 0.0034 0.004 0.0014 0.0033 0.0003 0.0011 0.0006 0.0016 0.0016 0.0041 0.0001 0.0019 0.0017
Endosome 0.0938 0.0837 0.059 0.0687 0.0981 0.0384 0.0872 0.091 0.0715 0.0369 0.1226 0.0555 0.1492 0.1408 0.0917 0.0124 0.1006 0.0963
Golgi 0.0543 0.069 0.0445 0.0276 0.0437 0.0379 0.062 0.0745 0.0517 0.0517 0.0717 0.053 0.0996 0.1412 0.1064 0.0214 0.0822 0.1136
Lipid Particles 0.0634 0.0262 0.0301 0.0235 0.0248 0.0241 0.0485 0.0176 0.0217 0.026 0.0357 0.0335 0.0823 0.0244 0.0115 0.023 0.1152 0.0717
Mitochondria 0.5438 0.589 0.6422 0.7547 0.7226 0.7107 0.521 0.5643 0.6511 0.7128 0.6417 0.6434 0.4094 0.3665 0.4425 0.6911 0.4309 0.3857
None 0.0084 0.0038 0.0206 0.0013 0.0007 0.0136 0.037 0.0223 0.0248 0.0331 0.0064 0.029 0.0095 0.0597 0.0449 0.0003 0.0233 0.0014
Nuclear Periphery 0.003 0.0003 0.0054 0.0005 0.002 0.0018 0.0019 0.0003 0.0028 0.0002 0.0005 0.0003 0.0006 0.0005 0.0011 0.0001 0.0155 0.0024
Nucleolus 0.0039 0.0005 0.0055 0.0002 0.0003 0.0011 0.0014 0.002 0.0022 0.0004 0.0008 0.0004 0.0007 0.0004 0.0003 0.0001 0.0006 0.0012
Nucleus 0.0032 0.0016 0.0056 0.0003 0.0005 0.0018 0.0018 0.0008 0.001 0.0003 0.0007 0.0003 0.0004 0.0005 0.0006 0.0001 0.0169 0.0011
Peroxisomes 0.0844 0.0756 0.07 0.0321 0.0603 0.0859 0.0484 0.0791 0.0568 0.0805 0.0324 0.0965 0.077 0.0583 0.0681 0.1821 0.0594 0.0796
Punctate Nuclear 0.0034 0.0004 0.0011 0.0003 0.0004 0.0022 0.0065 0.0019 0.0017 0.0003 0.0003 0.0008 0.002 0.0004 0.0006 0.0009 0.0074 0.0033
Vacuole 0.008 0.006 0.0057 0.0056 0.0052 0.0024 0.0057 0.0047 0.0085 0.0028 0.0063 0.0036 0.0054 0.0067 0.0046 0.0004 0.0013 0.0021
Vacuole Periphery 0.0033 0.0028 0.0039 0.0037 0.0042 0.0026 0.0032 0.0023 0.004 0.0024 0.0042 0.0032 0.004 0.0029 0.0062 0.0005 0.0013 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8475 3.7917 1.2058 5.2805 3.9281 6.6616 3.423 5.1751 5.6921 5.9919
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2288 1101 859 1545 1755 1988 154 172 4043 3089 1013 1717

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 659.78 638.44 777.54 810.01 712.24 740.04 954.33 858.59 682.55 703.83 804.42 814.88
Standard Deviation 76.56 86.06 129.55 98.12 85.53 101.32 100.00 97.34 84.67 107.77 140.64 99.12
Intensity Change Log 2 -0.047434 0.236932 0.295955 0.055240 0.422125 0.269606 0.006777 0.336029 0.282337

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000758 0.002956 0.006014 0.022140 0.000700 0.001965 0.030455 0.022819 0.000733 0.002318 0.009730 0.022208
Bud Neck 0.029165 0.015399 0.009588 0.027847 0.004161 0.006215 0.021517 0.022470 0.018312 0.009489 0.011402 0.027308
Bud Site 0.027932 0.022271 0.053349 0.106635* 0.006899 0.010943 0.059376 0.083344 0.018802 0.014980 0.054265 0.104301*
Cell Periphery 0.000603 0.001474 0.000275 0.000369 0.000235 0.000235 0.000921 0.000389 0.000443 0.000677 0.000373 0.000371
Cytoplasm 0.024559 0.012688 0.024837 0.065590 0.002501 0.005415 0.034733 0.048015 0.014984 0.008008 0.026342 0.063830
Cytoplasmic Foci 0.100826 0.077654 0.135929 0.141071 0.048547 0.067386 0.205343* 0.167884 0.078133 0.071046 0.146481 0.143757
Eisosomes 0.000159 0.000172 0.000129 0.000212 0.000072 0.000097 0.000272 0.000205 0.000121 0.000124 0.000151 0.000211
Endoplasmic Reticulum 0.000716 0.000547 0.005257 0.002517 0.000251 0.000223 0.005113 0.002830 0.000514 0.000339 0.005235 0.002548
Endosome 0.081208 0.089001 0.110011 0.074737 0.079606 0.111618 0.121777 0.104086 0.080512 0.103557 0.111799 0.077677
Golgi 0.100852 0.137402* 0.133349* 0.053316 0.139377* 0.187152* 0.066151 0.058017 0.117575* 0.169420* 0.123133* 0.053787
Lipid Particles 0.010407 0.007050 0.006831 0.004767 0.003092 0.003223 0.016827 0.012647 0.007232 0.004587 0.008351 0.005557
Mitochondria 0.529794* 0.503684* 0.245610* 0.133327* 0.647366* 0.516761* 0.051252 0.043961 0.580830* 0.512100* 0.216063* 0.124375*
Mitotic Spindle 0.008672 0.011202 0.083984 0.089828 0.007369 0.015533 0.060063 0.080129 0.008106 0.013989 0.080347 0.088856
None 0.002037 0.001942 0.000920 0.001546 0.000235 0.000397 0.000891 0.000250 0.001255 0.000948 0.000916 0.001416
Nuclear Periphery 0.000124 0.000072 0.000975 0.000416 0.000082 0.000062 0.001175 0.000323 0.000106 0.000066 0.001006 0.000406
Nuclear Periphery Foci 0.000308 0.000349 0.003208 0.000824 0.000164 0.000143 0.004989 0.000889 0.000246 0.000217 0.003479 0.000830
Nucleolus 0.001633 0.001753 0.003119 0.001028 0.000545 0.000532 0.004714 0.000558 0.001161 0.000968 0.003361 0.000981
Nucleus 0.003191 0.002403 0.012551 0.011597 0.000467 0.000840 0.025409 0.010362 0.002009 0.001397 0.014506 0.011474
Peroxisomes 0.043845 0.093981 0.052099 0.156912* 0.040682 0.047143 0.196132* 0.245875* 0.042472 0.063837 0.073995 0.165824*
Vacuole 0.017995 0.008480 0.072741 0.096429 0.007313 0.012925 0.083003 0.089152 0.013358 0.011341 0.074301 0.095700
Vacuole Periphery 0.015215 0.009517 0.039224 0.008893 0.010333 0.011189 0.009886 0.005794 0.013096 0.010593 0.034764 0.008583

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.99 -6.30 -10.53 -8.82 -7.15 -3.75 -3.39 -3.49 -3.26 0.97 -4.65 -5.90 -11.11 -10.10 -4.73
Bud Neck 5.28 9.01 -0.12 -5.77 -10.12 -1.86 -5.13 -4.59 -4.07 -0.36 6.23 4.54 -5.44 -10.95 -9.41
Bud Site 2.23 -6.14 -16.53 -17.49 -8.68 -2.66 -5.89 -7.11 -6.71 -1.66 2.54 -9.71 -20.11 -21.04 -8.80
Cell Periphery -1.42 4.31 3.04 1.84 -1.68 -0.07 -1.76 -0.92 -0.91 1.37 -1.06 0.97 1.60 1.48 0.27
Cytoplasm 4.97 -0.54 -12.25 -15.60 -9.87 -4.24 -4.90 -6.39 -6.05 -2.42 5.50 -4.43 -16.64 -19.13 -10.09
Cytoplasmic Foci 5.21 -6.21 -7.73 -12.48 -0.60 -6.66 -12.08 -9.86 -8.21 2.42 2.71 -14.00 -15.39 -17.71 0.79
Eisosomes -0.62 1.62 -2.51 -2.07 -5.97 -3.89 -5.77 -4.45 -3.54 1.83 -0.21 -2.67 -6.08 -6.85 -4.08
Endoplasmic Reticulum 1.25 -7.72 -7.93 -8.50 4.10 0.41 -3.47 -3.00 -3.03 1.48 2.44 -8.74 -9.38 -10.22 4.38
Endosome -1.56 -5.22 2.25 3.41 6.93 -6.55 -3.26 -1.15 1.53 1.55 -6.59 -6.34 1.79 7.48 7.18
Golgi -5.96 -4.54 12.53 15.18 12.72 -7.19 7.66 9.16 13.83 1.08 -11.43 -0.59 18.64 27.36 12.34
Lipid Particles 1.93 2.38 5.08 1.40 1.68 -0.22 -4.09 -2.09 -2.03 1.19 3.00 -1.20 1.87 -1.26 2.46
Mitochondria 1.97 22.94 43.24* 30.87* 10.35 11.52 45.19* 52.75* 42.62* 0.84 8.10 34.91 60.89* 48.43* 9.52
Mitotic Spindle -1.49 -13.14 -16.97 -15.95 -0.97 -4.99 -4.66 -5.15 -4.51 -1.01 -4.79 -14.05 -17.94 -16.28 -1.41
None 0.07 2.37 0.66 0.43 -0.80 -1.36 -1.24 -1.11 0.07 0.91 0.77 1.06 -0.22 -0.63 -0.73
Nuclear Periphery 2.13 -4.23 -6.40 -7.50 2.32 0.83 -2.31 -3.30 -3.51 1.40 2.41 -4.85 -7.38 -8.37 2.68
Nuclear Periphery Foci -0.30 -4.17 -4.02 -3.24 3.20 0.35 -1.91 -4.30 -4.43 1.52 0.61 -4.59 -5.07 -5.19 3.52
Nucleolus -0.20 -1.75 1.09 0.82 2.35 -0.04 -2.34 -1.41 -1.29 2.01 0.52 -2.97 -0.32 -0.85 2.93
Nucleus 0.82 -4.78 -8.27 -7.97 -0.52 -1.44 -3.38 -4.07 -3.96 1.07 1.35 -6.26 -10.14 -10.47 0.19
Peroxisomes -8.70 -1.75 -17.04 -7.12 -13.62 -1.61 -7.26 -8.38 -8.08 -0.87 -6.72 -5.86 -19.27 -14.89 -11.03
Vacuole 6.97 -13.90 -20.12 -22.32 -6.34 -4.82 -7.32 -7.86 -7.49 -1.87 2.05 -16.66 -22.64 -23.06 -6.38
Vacuole Periphery 4.80 -8.72 6.28 1.14 11.47 -0.87 0.47 3.13 3.69 1.86 3.23 -9.26 5.91 3.18 11.41
Standard name
Human Ortholog
Description Peripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA
Localization
Cell Percentages mitochondrion (91%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Aep3

Aep3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aep3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available