Standard name
Human Ortholog
Description Cyclin-dependent kinase inhibitor; interacts with and inhibits the Cdc28p/Cln2p, G1/S phase cyclin-dependent kinase complex but not S-phase, or M-phase complexes; overexpression blocks cells in G1 phase and stabilizes the Cdc28p inhibitor Sic1p, while disruption accelerates the G1/S phase transition; phosphorylated during S phase in a Cdc28p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.93 0.95 0.95 0.92 0.8 0.64 0.7 0.56 0.64 0.5 0.45 0.97 0.95 0.93 0.94 0.94 0.95 0.85 0.91 0.76 0.92 0.9 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.06 0 0.1 0.12 0.33 0.52 0.37 0.56 0.4 0.67 0.71 0 0 0 0 0 0 0.08 0 0.11 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.08 0.11 0.07 0.12 0.08 0.07 0 0.07 0.1 0 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 1 1 5
Bud 0 0 3 1 4 7 21 3 10 10 14 9 1 0 0 1 0 0 0 0 0 0 7 13
Bud Neck 0 1 0 0 1 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 1 0 2 4
Bud Site 0 0 0 0 0 0 1 1 1 2 3 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 4 2 2 2 1 5 0 6 2 5 0 2 2 4 7 5 1 0 0 0 1 3
Cytoplasm 204 97 188 225 339 300 401 354 209 313 176 176 344 460 475 254 273 245 194 103 163 219 429 509
Endoplasmic Reticulum 2 1 1 0 1 1 3 3 0 1 0 3 1 0 1 8 6 12 1 0 6 0 0 1
Endosome 0 0 2 1 8 4 10 7 2 6 0 0 1 1 1 2 2 5 0 0 2 0 5 5
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 1 1 1
Mitochondria 17 6 8 24 46 122 325 185 207 198 235 273 0 1 2 3 4 7 18 5 22 5 7 18
Nucleus 0 2 0 0 3 1 9 7 7 5 8 4 0 3 2 7 3 0 0 0 0 1 1 1
Nuclear Periphery 0 1 0 0 0 1 3 2 2 0 3 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 1 1 5 2 4 3 1 1 0 0 1 0 0 1 2 0 2 0 3 13
Vac/Vac Membrane 1 1 1 4 7 20 50 54 27 61 29 27 13 35 52 7 19 13 2 1 4 2 3 6
Unique Cell Count 213 104 198 237 368 375 629 505 370 491 351 387 354 485 512 269 290 257 229 114 214 238 478 594
Labelled Cell Count 224 110 209 257 413 462 829 624 470 606 472 501 360 503 537 287 315 288 229 114 214 238 478 594


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.0 4.1 4.0 4.3 3.8 3.6 4.2 3.7 5.5 3.4 3.4 5.7 6.2 7.2 6.3 6.3 6.2 5.4 5.5 5.7
Std Deviation (1e-4) 0.5 0.8 0.8 1.5 1.9 1.4 1.3 3.1 1.6 5.8 1.5 1.0 1.4 1.7 2.3 1.4 1.2 1.4 1.4 1.5 1.8
Intensity Change (Log2) – – – -0.04 0.07 -0.13 -0.2 0.03 -0.17 0.41 -0.29 -0.27 0.46 0.59 0.79 0.61 0.62 0.58 0.39 0.4 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 3.3 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8682 4.3599 4.3085 2.8754 3.6921 4.051 1.5983 2.4778 2.6283 2.0019 2.3096 2.2703 1.0866 2.0902 2.147 3.3546 1.8165 2.1531
Actin 0.0101 0.0065 0.0071 0.0006 0.0016 0.0018 0.0584 0.0007 0.004 0.002 0.0005 0.0019 0.0147 0.0006 0.0051 0.0005 0.0073 0.0008
Bud 0.0109 0.0142 0.0194 0.002 0.0023 0.0014 0.0009 0.0044 0.0008 0.0101 0.0005 0.0003 0.005 0.0002 0.0004 0.0002 0.0009 0.0009
Bud Neck 0.0021 0.0027 0.0024 0.0014 0.0021 0.0061 0.0047 0.0003 0.0007 0.0011 0.0013 0.0048 0.0005 0.0003 0.0007 0.0009 0.001 0.0034
Bud Periphery 0.0032 0.0041 0.007 0.0016 0.003 0.001 0.001 0.0046 0.001 0.0106 0.0002 0.0002 0.0027 0.0001 0.0003 0.0001 0.0038 0.001
Bud Site 0.0136 0.0292 0.0108 0.0027 0.0008 0.0005 0.0114 0.0047 0.0039 0.0009 0.0006 0.0004 0.0046 0.0007 0.0064 0.0002 0.0005 0.0001
Cell Periphery 0.0005 0.0004 0.0004 0.0003 0.0001 0.0001 0.0005 0.0002 0.0001 0.0001 0 0 0.0002 0.0001 0.0001 0 0.0002 0
Cytoplasm 0.6085 0.6494 0.7295 0.6466 0.6526 0.7311 0.6342 0.8559 0.8289 0.8356 0.9053 0.8624 0.7804 0.8678 0.8208 0.879 0.7578 0.8838
Cytoplasmic Foci 0.0158 0.008 0.0114 0.0163 0.0085 0.0127 0.0397 0.0039 0.013 0.0162 0.0286 0.0155 0.01 0.0043 0.014 0.0056 0.0194 0.0061
Eisosomes 0.0002 0.0001 0.0001 0 0.0001 0 0.0005 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0052 0.0038 0.0037 0.0055 0.0042 0.0037 0.0062 0.0028 0.0048 0.0034 0.0032 0.0046 0.0064 0.0033 0.0064 0.0046 0.0083 0.0047
Endosome 0.0066 0.0027 0.0054 0.0053 0.0049 0.0061 0.0412 0.0019 0.0107 0.0101 0.0149 0.0115 0.0231 0.002 0.0128 0.0036 0.0346 0.0043
Golgi 0.0007 0.0003 0.0007 0.0004 0.0003 0.0012 0.0127 0.0001 0.007 0.006 0.0007 0.002 0.0065 0.0001 0.005 0.0001 0.0136 0.001
Lipid Particles 0.0005 0.0002 0.0004 0.0006 0.0004 0.0003 0.0115 0.0001 0.001 0.016 0.0017 0.0013 0.0124 0.0001 0.0049 0.0001 0.0306 0.0032
Mitochondria 0.0023 0.0005 0.0029 0.0009 0.0007 0.001 0.0061 0.0001 0.003 0.002 0.0001 0.0028 0.0066 0.0001 0.0051 0.0002 0.0175 0.0006
None 0.283 0.2054 0.1475 0.2514 0.2088 0.1497 0.105 0.1079 0.0976 0.0467 0.0051 0.0352 0.0898 0.0941 0.0857 0.0522 0.0338 0.0339
Nuclear Periphery 0.0033 0.0033 0.0036 0.0023 0.0044 0.0041 0.016 0.0014 0.0035 0.0039 0.0019 0.0158 0.0071 0.002 0.0051 0.0036 0.0077 0.0047
Nucleolus 0.0038 0.0009 0.0009 0.0036 0.0008 0.0004 0.0022 0.0001 0.0004 0.0002 0.0003 0.0002 0.0003 0.0001 0.0002 0.0001 0.0008 0.0001
Nucleus 0.0197 0.0508 0.0304 0.0444 0.0922 0.0634 0.0303 0.0089 0.013 0.0179 0.0152 0.033 0.019 0.0214 0.0133 0.0466 0.0287 0.0468
Peroxisomes 0.0009 0.0006 0.0014 0.0004 0.0003 0.0006 0.005 0.0001 0.0007 0.0083 0.0007 0.0006 0.0035 0.0001 0.0099 0.0001 0.0205 0.0004
Punctate Nuclear 0.0058 0.0147 0.0131 0.0098 0.0099 0.013 0.005 0.0009 0.0031 0.0055 0.0179 0.004 0.0034 0.0015 0.0015 0.0011 0.0043 0.0028
Vacuole 0.0028 0.0019 0.0018 0.0038 0.0017 0.0014 0.0055 0.0009 0.0021 0.0027 0.0012 0.0018 0.0026 0.0012 0.0016 0.001 0.0045 0.0012
Vacuole Periphery 0.0004 0.0002 0.0003 0.0003 0.0003 0.0002 0.002 0.0001 0.0005 0.0008 0.0002 0.0017 0.0013 0.0001 0.0006 0.0002 0.004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.777 6.025 2.5547 9.9045 9.3626 8.6607 4.7865 6.7548 7.5118 11.2616
Translational Efficiency 1.1326 0.4612 1.4948 0.6228 1.0842 1.557 1.0691 0.9698 1.2192 1.0528

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1719 178 215 875 822 186 215 433 2541 364 430 1308

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 677.90 772.45 1008.11 868.08 673.56 750.28 973.93 946.36 676.50 761.12 991.02 893.99
Standard Deviation 76.17 100.47 117.31 144.91 87.15 89.21 130.78 318.60 79.91 95.53 125.40 221.37
Intensity Change Log 2 — 0.188369 0.572509 0.356756 — 0.155623 0.532012 0.490583 — 0.172141 0.552467 0.425006

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000161 0.001147 0.000840 0.002612 0.000084 0.000529 0.000297 0.000380 0.000136 0.000831 0.000569 0.001873
Bud Neck 0.015042 0.052309 0.005304 0.007814 0.009713 0.042012 0.001926 0.004975 0.013318 0.047047 0.003615 0.006874
Bud Site 0.005370 0.037331 0.004935 0.022771 0.003126 0.009714 0.002877 0.022942 0.004644 0.023219 0.003906 0.022827
Cell Periphery 0.000174 0.000206 0.000346 0.000315 0.000182 0.000283 0.000253 0.000160 0.000176 0.000245 0.000300 0.000264
Cytoplasm 0.472950* 0.268145* 0.246119* 0.472627* 0.554302* 0.287287* 0.406288* 0.461929* 0.499267* 0.277926* 0.326204* 0.469085*
Cytoplasmic Foci 0.254323* 0.282881* 0.012101 0.039495 0.188758* 0.214828* 0.033103 0.015478 0.233113* 0.248107* 0.022602 0.031545
Eisosomes 0.000241 0.000184 0.000108 0.000102 0.000121 0.000195 0.000103 0.000043 0.000202 0.000189 0.000105 0.000083
Endoplasmic Reticulum 0.001491 0.001705 0.013341 0.005875 0.001058 0.001448 0.003813 0.002610 0.001351 0.001574 0.008577 0.004794
Endosome 0.008793 0.038412 0.005339 0.009216 0.010052 0.038417 0.006442 0.003100 0.009200 0.038415 0.005891 0.007191
Golgi 0.002520 0.005594 0.000128 0.002726 0.001052 0.008332 0.000107 0.000151 0.002045 0.006993 0.000117 0.001874
Lipid Particles 0.011299 0.005965 0.003391 0.002920 0.005338 0.009259 0.003385 0.001210 0.009371 0.007648 0.003388 0.002354
Mitochondria 0.005352 0.009939 0.000506 0.007547 0.001613 0.018060 0.000244 0.000750 0.004142 0.014089 0.000375 0.005297
Mitotic Spindle 0.001854 0.014679 0.002424 0.011766 0.002331 0.015235 0.000626 0.026722 0.002008 0.014963 0.001525 0.016717
None 0.002539 0.001876 0.002624 0.002326 0.005110 0.001589 0.003427 0.015235 0.003371 0.001729 0.003026 0.006599
Nuclear Periphery 0.000451 0.000481 0.002460 0.001452 0.000330 0.000979 0.001487 0.001173 0.000412 0.000736 0.001974 0.001360
Nuclear Periphery Foci 0.000807 0.001087 0.003934 0.003158 0.000621 0.000524 0.003488 0.001656 0.000747 0.000799 0.003711 0.002660
Nucleolus 0.000739 0.001744 0.000345 0.000284 0.001071 0.001884 0.000619 0.000564 0.000846 0.001815 0.000482 0.000377
Nucleus 0.171883* 0.112330 0.521499* 0.196600* 0.173862* 0.196765* 0.330246* 0.293667* 0.172523* 0.155475* 0.425873* 0.228733*
Peroxisomes 0.005238 0.044120 0.000328 0.002796 0.003181 0.014279 0.000362 0.000791 0.004572 0.028872 0.000345 0.002133
Vacuole 0.038161 0.117949 0.173726 0.202791* 0.037408 0.134413 0.200707* 0.145268 0.037917 0.126362 0.187217 0.183749
Vacuole Periphery 0.000615 0.001917 0.000203 0.004806 0.000687 0.003969 0.000199 0.001195 0.000638 0.002966 0.000201 0.003611

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.25 -8.67 -3.00 -1.09 -1.11 -2.07 -8.02 -4.46 -0.32 -0.69 -4.86 -10.58 -3.44 -1.43 -1.49
Bud Neck -4.55 4.41 5.42 5.63 1.29 -3.49 4.72 1.81 3.86 -3.67 -5.44 7.22 5.66 6.58 -1.09
Bud Site -4.71 -1.76 -4.71 3.10 -3.81 -3.12 -2.20 -3.57 -0.99 -3.02 -5.19 -1.78 -5.88 2.16 -5.23
Cell Periphery -1.16 -6.45 -3.36 -1.41 4.06 -1.93 -2.39 3.56 2.85 4.29 -2.26 -6.41 -2.22 1.05 4.57
Cytoplasm 10.47 18.03 8.76 -5.09 -10.61 13.47 11.88 15.24 -1.54 -0.19 16.07 19.02 15.65 -5.30 -7.28
Cytoplasmic Foci -1.32 45.11 40.81 14.90 -8.30 -1.89 23.65 26.96 14.75 3.45 -1.17 48.55 48.31 20.45 -2.96
Eisosomes 1.01 2.10 2.66 4.81 2.46 -3.04 0.20 7.90 5.38 5.95 0.27 2.17 3.17 6.62 4.48
Endoplasmic Reticulum -0.89 -11.07 -9.92 -5.71 7.78 -0.73 -7.28 -5.86 -5.55 3.04 -0.98 -12.56 -11.50 -8.46 7.54
Endosome -5.43 3.26 3.41 6.09 1.05 -5.28 2.99 5.93 6.86 4.88 -7.68 4.49 5.89 9.00 2.45
Golgi -2.00 7.11 2.18 2.90 -1.68 -1.93 6.62 6.48 2.18 -0.95 -2.34 8.28 2.86 2.95 -1.71
Lipid Particles 4.28 8.52 10.05 3.09 2.13 -3.02 3.44 8.28 6.13 4.99 1.78 9.33 11.74 6.40 3.90
Mitochondria -1.58 5.20 1.29 2.25 -2.20 -2.53 3.60 2.64 2.68 -3.44 -2.69 5.85 1.61 3.14 -2.39
Mitotic Spindle -1.94 -1.24 -4.48 0.08 -2.83 -1.50 -0.35 -4.45 -1.58 -4.31 -2.42 -1.27 -6.28 -0.82 -5.17
None 1.06 1.37 0.34 -0.86 -1.08 3.81 2.74 -1.71 -4.00 -3.37 3.38 2.60 -1.43 -3.88 -3.27
Nuclear Periphery -1.00 -11.43 -8.54 -7.75 4.50 -3.70 -12.79 -10.96 -5.36 -0.45 -3.35 -15.57 -12.43 -8.34 3.46
Nuclear Periphery Foci -0.73 -5.96 -6.19 -5.13 1.35 0.79 -5.27 -6.73 -6.88 3.23 -0.28 -7.58 -7.57 -7.58 2.77
Nucleolus -3.74 4.83 8.71 5.27 2.84 -2.34 2.47 2.27 3.93 -0.45 -4.50 4.01 5.77 6.60 1.38
Nucleus 3.78 -16.37 -5.84 -6.61 13.66 -1.40 -8.42 -9.17 -5.31 1.07 1.15 -17.13 -10.38 -7.10 11.15
Peroxisomes -5.93 11.76 5.49 6.54 -1.83 -2.55 4.46 3.10 3.11 -1.24 -6.09 12.12 6.33 6.97 -2.16
Vacuole -7.79 -11.72 -29.26 -13.22 -4.32 -8.66 -13.16 -17.44 -8.39 0.17 -11.69 -17.54 -33.85 -15.81 -2.89
Vacuole Periphery -3.57 9.04 -1.47 -0.13 -1.89 -2.79 4.51 0.99 2.97 -1.02 -3.67 9.39 -1.32 1.67 -2.01
Standard name
Human Ortholog
Description Cyclin-dependent kinase inhibitor; interacts with and inhibits the Cdc28p/Cln2p, G1/S phase cyclin-dependent kinase complex but not S-phase, or M-phase complexes; overexpression blocks cells in G1 phase and stabilizes the Cdc28p inhibitor Sic1p, while disruption accelerates the G1/S phase transition; phosphorylated during S phase in a Cdc28p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
Localization
Cell Percentages cytoplasm (33%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cip1

Cip1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cip1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available