Standard name
Human Ortholog
Description Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC3 has a paralog, VTC2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.75 0.81 0.71 0.8 0.7 0.8 0.79 0.78 – 0.83 0.79 0.78 0.89 0.93 0.98 0.59 0.59 0.6 0.4 0.49 0.29 0.45 0.38 0.49
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.13 0.17 0.17 – 0.28 0.35 0.36 0 0 0 0.07 0 0 0 0 0.06 0.06 0.08 0
Nucleus 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0.21 0.24 0.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.22 0.15 0.26 0.23 0.31 0.2 0.19 0.16 – 0.09 0.08 0.09 0.13 0.06 0 0.2 0.21 0.25 0.46 0.38 0.48 0.36 0.42 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 1 0 0 1 1 7 1 3 2
Bud 0 0 0 1 2 5 10 8 – 11 11 19 0 0 0 0 0 0 10 8 23 7 5 9
Bud Neck 0 0 1 0 0 0 1 2 – 0 0 1 0 0 3 1 0 0 2 1 6 5 0 2
Bud Site 0 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 0 1 0 1 – 1 1 0 0 0 2 0 0 0 0 0 1 0 0 0
Cytoplasm 213 148 374 259 278 488 515 728 – 599 411 486 73 201 268 199 129 93 116 91 163 99 94 143
Endoplasmic Reticulum 1 1 3 0 2 0 2 0 – 0 0 0 0 0 1 12 22 8 1 1 13 1 2 2
Endosome 7 1 16 1 3 4 1 6 – 2 0 1 0 1 0 8 3 4 3 5 14 3 3 5
Golgi 0 0 2 0 1 0 0 0 – 0 0 0 0 0 1 1 0 1 4 1 3 0 1 0
Mitochondria 1 10 7 11 14 77 113 161 – 198 182 224 1 0 0 25 9 3 6 2 32 13 18 8
Nucleus 12 3 23 12 20 21 18 28 – 6 4 6 1 3 0 72 53 31 1 0 0 0 0 1
Nuclear Periphery 2 0 3 0 3 1 5 9 – 7 3 8 0 0 0 1 0 0 0 0 2 0 0 1
Nucleolus 0 0 0 0 1 0 1 2 – 1 0 0 0 1 0 1 0 0 0 0 1 0 0 2
Peroxisomes 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 1 3 4 – 3 1 5 0 0 0 1 0 1 5 1 10 2 7 6
Vac/Vac Membrane 62 28 136 75 124 123 127 145 – 65 42 58 11 12 6 68 46 39 135 71 267 80 101 99
Unique Cell Count 283 182 526 324 397 610 654 932 719 523 625 82 215 274 336 217 155 293 187 560 222 245 292
Labelled Cell Count 299 191 566 360 448 721 797 1094 894 656 809 86 218 281 390 262 180 293 187 560 222 245 292


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.7 5.0 4.7 4.6 3.5 3.3 3.5 – 3.0 2.8 2.9 5.6 5.2 4.8 5.5 5.8 6.9 5.7 5.9 5.7
Std Deviation (1e-4) 2.3 2.0 2.2 2.4 2.2 1.3 1.5 1.2 – 0.9 0.8 0.9 1.6 1.3 1.1 2.0 2.2 3.1 2.2 1.8 1.9
Intensity Change (Log2) – – – -0.08 -0.12 -0.52 -0.59 -0.51 – -0.76 -0.82 -0.78 0.15 0.06 -0.06 0.12 0.2 0.45 0.19 0.23 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -1.9 -1.3 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0669 0.7819 0.9727 0.0751 0.62 0.6562 2.2235 2.5282 2.4284 3.009 1.8281 2.3062 2.1664 2.5809 2.0497 2.3408 2.2953 2.2834
Actin 0.0502 0.0045 0.0304 0.0001 0.0235 0.0069 0.0506 0.0001 0.0091 0.0001 0.0003 0.0001 0.0465 0.0001 0.0031 0.001 0.0001 0.0006
Bud 0.0035 0.0027 0.0009 0.0003 0.0006 0.0032 0.0023 0.0023 0.0024 0.0002 0.0002 0.0005 0.0007 0.0001 0.0007 0.0006 0 0.0004
Bud Neck 0.0015 0.0017 0.0021 0.0002 0.001 0.017 0.0038 0.0001 0.0006 0.0011 0.0004 0.0039 0.011 0.0001 0.0004 0.0206 0 0.0005
Bud Periphery 0.0091 0.0018 0.0016 0.0004 0.0008 0.0044 0.0041 0.0018 0.0025 0.0004 0.0002 0.0007 0.001 0.0001 0.0026 0.0011 0 0.0006
Bud Site 0.0035 0.0154 0.0069 0.0004 0.0061 0.0013 0.0031 0.0116 0.0065 0.0008 0.0002 0.0003 0.0121 0.0003 0.0012 0.0183 0 0.0001
Cell Periphery 0.0041 0.0019 0.0003 0.0001 0.0002 0.0022 0.0006 0.0003 0.0004 0.011 0.0001 0.0008 0.0005 0.0003 0.0016 0.0007 0.0001 0.0011
Cytoplasm 0.0728 0.1767 0.1096 0.2257 0.0815 0.2221 0.249 0.246 0.0956 0.1876 0.3012 0.2094 0.0777 0.1762 0.1569 0.1665 0.1548 0.2176
Cytoplasmic Foci 0.0301 0.0137 0.0302 0.0476 0.0391 0.0304 0.0358 0.0069 0.0489 0.0473 0.0586 0.0339 0.0178 0.0098 0.0169 0.0303 0.0035 0.0168
Eisosomes 0.0003 0.0001 0.0003 0.0001 0.0004 0.0001 0.0003 0.0003 0.0002 0.0001 0 0 0.0007 0.0002 0.0001 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.009 0.0027 0.0048 0.0048 0.0014 0.0045 0.0499 0.0068 0.0037 0.0073 0.003 0.0034 0.0066 0.0041 0.0024 0.0025 0.0016 0.0014
Endosome 0.0455 0.0125 0.048 0.1027 0.0157 0.0351 0.1134 0.0184 0.0944 0.1096 0.0308 0.0475 0.0157 0.0092 0.0272 0.0357 0.0015 0.0119
Golgi 0.0053 0.0008 0.0083 0.0013 0.0103 0.0053 0.0129 0.0005 0.0157 0.0015 0.0005 0.001 0.0111 0.0002 0.0079 0.001 0.0001 0.0002
Lipid Particles 0.006 0.0011 0.009 0.0046 0.0208 0.0136 0.0044 0.0012 0.0085 0.0045 0.0027 0.004 0.0062 0.002 0.0024 0.0023 0.0002 0.0006
Mitochondria 0.0028 0.0007 0.0057 0.0018 0.0045 0.0034 0.0054 0.0005 0.0055 0.001 0.0004 0.0007 0.0208 0.0004 0.0032 0.0032 0.0001 0.0007
None 0.6383 0.7136 0.6563 0.5727 0.712 0.5817 0.3191 0.6833 0.6788 0.4501 0.5537 0.6291 0.7264 0.7817 0.7498 0.6733 0.8361 0.7231
Nuclear Periphery 0.0247 0.0014 0.013 0.004 0.0031 0.0033 0.046 0.0019 0.0015 0.0036 0.0024 0.001 0.0027 0.0009 0.0016 0.0026 0.0002 0.0005
Nucleolus 0.0021 0.0009 0.0031 0.0008 0.0011 0.0022 0.0008 0.0009 0.0008 0.0009 0.0003 0.0005 0.0015 0.0002 0.0005 0.0009 0 0.0002
Nucleus 0.019 0.0038 0.0216 0.0034 0.0017 0.0065 0.0067 0.0039 0.0011 0.0045 0.0016 0.0015 0.0017 0.0012 0.0013 0.0021 0.0002 0.0012
Peroxisomes 0.0056 0.0012 0.0082 0.0006 0.0205 0.009 0.0012 0.0001 0.0076 0.0005 0.0004 0.0005 0.032 0.0001 0.0012 0.0008 0.0001 0.0003
Punctate Nuclear 0.0113 0.0024 0.0152 0.0041 0.0085 0.0039 0.0035 0.0015 0.002 0.0012 0.0072 0.0013 0.004 0.0003 0.0027 0.0013 0.0001 0.0005
Vacuole 0.0491 0.039 0.0194 0.0209 0.0461 0.0405 0.0801 0.0108 0.012 0.1633 0.0335 0.0572 0.0027 0.0122 0.0149 0.029 0.0009 0.0208
Vacuole Periphery 0.006 0.0015 0.005 0.0034 0.0011 0.0034 0.0067 0.0009 0.0022 0.0037 0.0023 0.0024 0.0006 0.0003 0.0016 0.0058 0.0001 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 215.7974 239.5148 293.5906 331.3265 244.71 197.8697 315.4415 322.6068 326.6392 370.7806
Translational Efficiency 0.9941 1.2297 1.1233 0.9827 0.9889 1.1906 1.0286 1.0353 1.044 1.0152

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
177 1724 99 1455 2006 2030 190 1771 2183 3754 289 3226

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 842.77 809.32 843.37 767.53 882.20 781.78 859.17 817.19 879.00 794.43 853.76 794.79
Standard Deviation 148.93 148.27 88.54 139.71 154.50 144.33 92.53 117.45 154.43 146.80 91.49 130.33
Intensity Change Log 2 — -0.058429 0.001027 -0.134916 — -0.174343 -0.038162 -0.110434 — -0.116547 -0.018883 -0.122343

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.008321 0.000088 0.000801 0.001276 0.000010 0.000365 0.000878 0.000634 0.000684 0.000238 0.000852 0.000923
Bud Neck 0.005480 0.022533 0.002165 0.010181 0.006868 0.051400 0.007571 0.014742 0.006756 0.038143 0.005719 0.012685
Bud Site 0.031652 0.017887 0.007363 0.048300 0.004088 0.031443 0.008557 0.042175 0.006323 0.025217 0.008148 0.044937
Cell Periphery 0.002266 0.000709 0.000243 0.000756 0.003959 0.002819 0.000275 0.000245 0.003822 0.001850 0.000264 0.000476
Cytoplasm 0.253084* 0.323577* 0.225595* 0.513532* 0.013866 0.247048* 0.213916* 0.546162* 0.033262 0.282194* 0.217916* 0.531445*
Cytoplasmic Foci 0.105580 0.062743 0.030285 0.035968 0.003769 0.127774 0.021668 0.026394 0.012024 0.097909 0.024620 0.030712
Eisosomes 0.000180 0.000039 0.000108 0.000087 0.000017 0.000168 0.000122 0.000064 0.000030 0.000109 0.000117 0.000074
Endoplasmic Reticulum 0.005864 0.002515 0.004363 0.002402 0.008643 0.004826 0.007620 0.001265 0.008418 0.003765 0.006504 0.001777
Endosome 0.040548 0.024532 0.007162 0.005440 0.023732 0.043099 0.005422 0.004691 0.025095 0.034572 0.006018 0.005029
Golgi 0.023211 0.001715 0.000125 0.003264 0.000293 0.005296 0.000110 0.001945 0.002151 0.003651 0.000115 0.002540
Lipid Particles 0.004944 0.002139 0.004329 0.002119 0.000887 0.006650 0.004308 0.001181 0.001216 0.004578 0.004315 0.001604
Mitochondria 0.005023 0.004099 0.000705 0.002109 0.000478 0.016438 0.000659 0.003734 0.000846 0.010772 0.000675 0.003001
Mitotic Spindle 0.003748 0.003100 0.000564 0.030050 0.000041 0.006263 0.001012 0.027943 0.000341 0.004810 0.000859 0.028893
None 0.002728 0.001667 0.003088 0.004979 0.000555 0.001524 0.003882 0.002957 0.000732 0.001590 0.003610 0.003869
Nuclear Periphery 0.000710 0.000318 0.001664 0.000847 0.000204 0.000429 0.002089 0.000888 0.000245 0.000378 0.001943 0.000870
Nuclear Periphery Foci 0.001855 0.000343 0.001655 0.002300 0.000239 0.000388 0.003320 0.000522 0.000370 0.000367 0.002750 0.001324
Nucleolus 0.000675 0.000749 0.001609 0.000878 0.000636 0.001096 0.000925 0.000684 0.000639 0.000936 0.001159 0.000771
Nucleus 0.101166 0.062110 0.622651* 0.171200* 0.004016 0.045399 0.605427* 0.199907* 0.011893 0.053073 0.611327* 0.186960*
Peroxisomes 0.003429 0.002504 0.001660 0.002449 0.000173 0.011849 0.002701 0.001194 0.000437 0.007557 0.002344 0.001760
Vacuole 0.391480* 0.457635* 0.083497 0.160365 0.907504* 0.384879* 0.109330 0.119894 0.865664* 0.418292* 0.100481 0.138147
Vacuole Periphery 0.008055 0.008998 0.000369 0.001499 0.020022 0.010846 0.000208 0.002776 0.019052 0.009998 0.000263 0.002200

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.60 1.38 1.33 -3.63 -0.39 -5.07 -3.54 -4.14 -1.03 1.21 0.77 -1.04 -0.90 -3.60 0.35
Bud Neck -9.03 1.89 -2.48 7.87 -5.08 -16.54 0.62 -4.50 13.68 -2.41 -18.19 1.33 -4.12 15.60 -3.11
Bud Site 1.47 3.01 -0.63 -3.66 -4.99 -14.28 -0.63 -10.87 0.44 -6.70 -14.16 -0.12 -12.36 -2.14 -8.32
Cell Periphery 0.69 1.06 0.91 1.54 -1.03 1.97 10.98 11.25 5.31 2.80 4.63 10.81 10.50 5.53 -0.32
Cytoplasm -4.00 1.32 -9.09 -9.17 -9.57 -36.29* -12.98 -68.88* -26.16 -18.53 -44.83* -13.95 -75.79* -24.74 -20.09
Cytoplasmic Foci 2.78 5.93 7.13 10.20 0.09 -29.42* -5.77 -16.03 23.83 -1.49 -30.57 -4.47 -11.80 25.08 -1.16
Eisosomes 6.76 1.65 4.23 -11.13 3.12 -12.51 -11.17 -16.53 8.36 6.74 -11.50 -12.26 -14.95 4.98 7.13
Endoplasmic Reticulum 1.03 -0.61 0.85 -1.29 3.66 5.51 -1.11 14.39 6.23 8.07 8.25 -0.25 12.94 5.09 8.56
Endosome 1.16 4.53 5.78 13.30 2.24 -8.78 12.20 14.51 21.94 2.13 -5.86 12.10 15.82 25.36 3.10
Golgi 2.18 2.45 2.20 0.19 -2.16 -7.90 2.96 -1.88 5.24 -2.27 -3.38 2.72 0.22 4.13 -3.13
Lipid Particles 2.70 -0.44 3.69 0.34 5.02 -7.60 -7.61 -2.16 7.15 6.95 -6.82 -9.07 -2.43 6.17 8.36
Mitochondria -0.31 1.38 1.06 2.61 -1.66 -10.14 -0.22 -3.35 8.28 -3.28 -10.10 0.61 -3.18 8.48 -3.78
Mitotic Spindle 0.16 2.97 -5.59 -5.59 -6.84 -5.34 -1.86 -8.65 -5.52 -8.28 -5.81 -1.21 -11.02 -7.85 -10.71
None 0.49 -0.33 -0.79 -4.16 -0.89 -2.69 -3.65 -5.19 -3.52 0.12 -2.63 -4.16 -6.17 -5.44 -0.55
Nuclear Periphery 5.57 -5.45 -5.36 -13.29 2.01 -2.19 -14.69 -16.02 -8.70 6.33 -1.91 -16.84 -19.35 -14.69 6.28
Nuclear Periphery Foci 5.62 -1.18 -0.43 -5.76 0.87 -1.21 -3.32 -5.00 -1.80 3.08 0.01 -3.91 -6.14 -5.95 3.02
Nucleolus -1.33 -2.30 -0.04 0.93 2.14 -1.64 -0.49 -0.02 2.70 0.79 -1.24 -1.29 0.06 2.61 2.16
Nucleus 3.65 -14.02 -5.03 -22.50 12.80 -18.33 -28.43 -41.87 -31.76 16.77 -17.24 -33.62 -49.10 -38.55 21.27
Peroxisomes 0.10 0.98 0.94 1.41 -0.16 -12.25 -1.49 -3.70 11.42 0.83 -12.80 -1.72 -2.95 10.49 0.55
Vacuole -0.73 10.28 6.98 16.37 -6.90 57.44* 51.29* 113.22* 20.28 -0.86 58.05* 58.42* 101.62* 25.86 -5.37
Vacuole Periphery -1.35 1.98 1.82 10.42 -1.60 7.72 19.60 16.48 11.36 -3.27 8.39 19.45 17.67 15.33 -3.71
Standard name
Human Ortholog
Description Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC3 has a paralog, VTC2, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (86%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vtc3

Vtc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vtc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available