Standard name
Human Ortholog
Description Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 – 0 0 0 0.05 0.12 0.16 0.06 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0 0.06 0.08 0.05 0 0 0 – 0 0 0 0.16 0.24 0.23 0.28 0.29 0.3 0.13 0.16 0.18 0.17 0.16 0.19
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.35 0.81 0.75 0.73 0.86 0.91 0.83 – 0.83 0.86 0.9 0.24 0.13 0.06 0.13 0.1 0.18 0.06 0.05 0 0.08 0.11 0.1
Nucleus 0 0 0 0 0 0.07 0 0.06 – 0 0.06 0 0.18 0 0.05 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.66 0.48 0.54 0.36 0.36 0.23 0.26 0.15 – 0.28 0.17 0.23 0.42 0.56 0.47 0.5 0.54 0.48 0.67 0.57 0.69 0.45 0.5 0.48
Nucleolus 0.28 0.12 0.19 0.1 0.11 0.07 0.12 0.12 – 0.15 0.14 0.15 0.07 0.09 0.05 0.16 0.2 0.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0.06 0 0 0.06 0 0
Vac/Vac Membrane 0.14 0.35 0.05 0.08 0.11 0.07 0 0.05 – 0 0 0 0.14 0.21 0.23 0.27 0.28 0.26 0 0.05 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 – 0 0 0 1 1 1 1 0 0 0 0 0 0 1 3
Bud 0 0 0 2 2 11 11 15 – 7 13 12 1 1 0 0 1 0 1 2 0 0 1 1
Bud Neck 0 0 0 0 0 1 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 2 0 3
Bud Site 0 0 0 1 3 9 6 7 – 6 8 13 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 3 8 13 20 8 9 – 13 4 9 7 33 51 13 17 10 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 0 1 1 2 2 1 – 2 2 5 2 1 5 10 9 7 0 0 0 0 2 2
Endosome 8 1 5 25 20 7 11 10 – 7 3 6 24 64 74 64 72 68 15 17 18 14 40 53
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 3 1 0 2 6
Mitochondria 50 32 66 246 318 377 559 474 – 435 338 511 36 34 18 31 24 42 6 5 3 6 27 26
Nucleus 2 2 0 14 17 31 22 35 – 19 24 25 27 6 15 4 5 6 1 0 0 1 5 4
Nuclear Periphery 66 44 44 119 158 99 156 86 – 146 68 132 64 150 149 114 134 110 77 61 71 37 125 133
Nucleolus 28 11 15 33 47 32 74 71 – 79 56 82 10 24 16 37 49 46 1 2 0 3 7 7
Peroxisomes 0 0 0 5 1 3 4 5 – 6 5 1 1 1 7 0 2 1 3 0 2 4 9 6
SpindlePole 0 0 2 5 7 6 15 16 – 20 5 10 8 8 14 10 9 10 6 4 3 5 11 8
Vac/Vac Membrane 14 32 4 27 49 29 14 29 – 12 14 10 22 56 73 63 69 59 1 5 1 3 10 11
Unique Cell Count 100 91 81 328 435 436 611 571 522 391 565 153 266 319 230 246 229 116 108 103 84 252 276
Labelled Cell Count 170 126 139 486 636 629 883 759 752 540 816 203 379 423 347 393 360 116 108 103 84 252 276


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.9 4.2 4.5 4.6 4.3 3.6 3.8 – 3.6 3.7 3.8 6.3 6.4 6.5 6.9 7.0 7.2 7.2 6.9 7.3
Std Deviation (1e-4) 0.8 0.9 0.7 1.1 1.4 1.5 0.8 0.7 – 1.0 0.9 0.9 1.1 1.3 1.3 1.2 1.2 1.4 1.8 1.5 1.4
Intensity Change (Log2) – – – 0.08 0.12 0.02 -0.25 -0.15 – -0.23 -0.18 -0.15 0.58 0.6 0.61 0.71 0.73 0.76 0.76 0.7 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 – – – – – – 0 0 0 0 0 0
Bud Neck 0 0 0 – – – – – – 0 0 0 0 0 0
Bud Site 0 0 0 – – – – – – 0 0 0 0 0 0
Cell Periphery 0 0 0 – – – – – – 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 – – – – – – 0 0 0 0 0 0
Endosome 0 0 0 – – – – – – 2.1 3.5 3.4 4.0 4.2 4.3
Golgi 0 0 0 – – – – – – 0 0 0 0 0 0
Mitochondria 0 0 0 – – – – – – -8.5 -12.0 -15.0 -11.4 -12.5 -10.3
Nuclear Periphery 0 0 0 – – – – – – -1.8 0.3 -1.2 -0.7 0 -1.0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 – – – – – – -2.8 -2.4 -4.1 -0.5 0.3 0.3
Peroxisomes 0 0 0 – – – – – – 0 0 0 0 0 0
SpindlePole 0 0 0 – – – – – – 0 0 0 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 – – – – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 – – – – – – 0 2.2 2.9 0 0 0
Nucleus 0 0 0 – – – – – – 4.0 0 0 0 0 0
Vacuole 1.0 1.7 0.6 – – – – – – 2.2 3.4 3.7 4.2 4.3 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.159 2.144 2.0206 1.7065 1.7739 2.0923 2.443 2.7592 2.5282 2.2306 2.1266 2.6685 0.3405 0.5952 0.3157 0.218 0.5874 0.2895
Actin 0.0089 0.0005 0.0009 0.0006 0.0006 0.0003 0.0016 0.0006 0.0029 0.0094 0.0029 0.0004 0.0092 0.0016 0.0173 0.0666 0.0016 0.0008
Bud 0.0001 0.0001 0.0032 0 0 0 0 0 0.0001 0.0001 0 0 0.0002 0.0001 0.0013 0.0006 0 0
Bud Neck 0.0035 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0002 0.0002 0.0003 0.0005 0.0003 0.0004 0.001 0 0.0002
Bud Periphery 0.0002 0.0003 0.0007 0 0 0.0001 0.0001 0.0001 0.0003 0.0004 0.0002 0.0001 0.0007 0.0004 0.0019 0.0011 0.0001 0.0002
Bud Site 0.001 0.0004 0.0007 0 0 0 0.0003 0.0001 0.0006 0.0002 0.0001 0.0001 0.0007 0.0003 0.0093 0.002 0 0
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0001 0 0.0001 0.0001 0 0 0.0002 0.0001 0.0002 0.0001 0 0.0001
Cytoplasm 0.0014 0.0004 0.0009 0.0002 0.0002 0.0002 0.001 0.0002 0.0004 0.001 0.0008 0.0003 0.0102 0.0002 0.0004 0.0026 0.0002 0.0003
Cytoplasmic Foci 0.0018 0.0007 0.004 0.0003 0.0011 0.0004 0.0028 0.0012 0.0028 0.0017 0.0035 0.0007 0.0038 0.0023 0.0118 0.0572 0.0032 0.001
Eisosomes 0.0001 0.0001 0 0 0 0 0.0001 0 0.0002 0.0004 0.0001 0 0.0002 0.0001 0.0003 0.0007 0.0001 0.0001
Endoplasmic Reticulum 0.0017 0.0009 0.0012 0.0005 0.0004 0.0004 0.0019 0.0011 0.0012 0.0022 0.0011 0.0007 0.015 0.0018 0.0017 0.0019 0.0014 0.0031
Endosome 0.0077 0.002 0.0041 0.0011 0.0017 0.0019 0.0049 0.0027 0.003 0.0042 0.0067 0.0024 0.0157 0.0034 0.0076 0.0132 0.0017 0.002
Golgi 0.002 0.0006 0.0011 0.0004 0.0014 0.0007 0.0018 0.0014 0.0018 0.0031 0.0035 0.001 0.0062 0.0038 0.0139 0.0112 0.0051 0.0025
Lipid Particles 0.027 0.0124 0.0204 0.0063 0.0357 0.0133 0.033 0.0346 0.0332 0.0303 0.089 0.0219 0.0952 0.0915 0.0949 0.0257 0.256 0.0574
Mitochondria 0.0013 0.0047 0.0012 0.0006 0.0009 0.0006 0.0016 0.0014 0.0022 0.0037 0.0026 0.0006 0.0055 0.0046 0.0118 0.0042 0.0026 0.0055
None 0.0033 0.0005 0.0017 0.0006 0.0006 0.0004 0.0027 0.0006 0.002 0.0318 0.0053 0.0006 0.0121 0.001 0.0019 0.0195 0.0011 0.0004
Nuclear Periphery 0.8636 0.8258 0.857 0.9155 0.7897 0.8932 0.8742 0.868 0.8732 0.8487 0.7938 0.8897 0.7314 0.7629 0.7531 0.6407 0.682 0.8635
Nucleolus 0.0068 0.0352 0.0217 0.0048 0.0215 0.024 0.0073 0.0083 0.0087 0.0097 0.0111 0.0152 0.0219 0.0304 0.0064 0.01 0.0048 0.0068
Nucleus 0.0239 0.0538 0.0355 0.0195 0.0764 0.0253 0.0196 0.0159 0.012 0.0154 0.0064 0.0204 0.0321 0.0297 0.0065 0.0115 0.0077 0.0112
Peroxisomes 0.0011 0.0004 0.0011 0.0003 0.0005 0.0002 0.0012 0.0004 0.0027 0.0017 0.0028 0.0003 0.0014 0.0013 0.0107 0.0654 0.0018 0.0005
Punctate Nuclear 0.0329 0.0503 0.029 0.0401 0.0596 0.0327 0.031 0.0455 0.0353 0.0203 0.0593 0.0371 0.0178 0.0362 0.0302 0.044 0.0188 0.0284
Vacuole 0.001 0.0021 0.0007 0.0002 0.0002 0.0003 0.0011 0.0007 0.0006 0.0008 0.0006 0.0005 0.0026 0.0014 0.001 0.0007 0.0003 0.0019
Vacuole Periphery 0.0108 0.0087 0.0146 0.0089 0.0092 0.0058 0.0134 0.0172 0.0166 0.0147 0.0099 0.0075 0.0174 0.0266 0.0175 0.02 0.0112 0.0142

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.1221 9.5149 11.3752 16.9469 13.4168 14.7748 13.2282 14.2489 16.1937 17.2278
Translational Efficiency 0.7319 0.9862 0.9027 0.7526 0.7184 0.8044 0.8787 0.9704 0.951 0.7748

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
141 1883 54 216 1156 1349 176 2132 1297 3232 230 2348

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 744.18 822.00 1029.44 972.40 681.46 768.21 1061.75 843.83 688.28 799.55 1054.16 855.66
Standard Deviation 88.35 112.76 128.89 112.27 106.47 106.59 112.26 107.09 106.46 113.38 117.18 113.81
Intensity Change Log 2 — 0.143487 0.468136 0.385898 — 0.172872 0.639743 0.308323 — 0.157608 0.552714 0.349337

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000300 0.000147 0.001219 0.000519 0.000276 0.000156 0.000245 0.000136 0.000279 0.000151 0.000474 0.000171
Bud Neck 0.000097 0.000143 0.002167 0.000862 0.000406 0.000289 0.000709 0.000364 0.000372 0.000204 0.001051 0.000410
Bud Site 0.000126 0.000056 0.000777 0.000181 0.000183 0.000253 0.000109 0.000152 0.000177 0.000138 0.000266 0.000154
Cell Periphery 0.000046 0.000027 0.000165 0.000031 0.000086 0.000050 0.000021 0.000021 0.000081 0.000037 0.000055 0.000022
Cytoplasm 0.003622 0.000637 0.020730 0.000465 0.007564 0.002546 0.000152 0.001248 0.007135 0.001434 0.004984 0.001176
Cytoplasmic Foci 0.003488 0.002807 0.012262 0.000399 0.011201 0.006272 0.000442 0.001296 0.010362 0.004253 0.003217 0.001214
Eisosomes 0.000021 0.000008 0.000080 0.000011 0.000031 0.000018 0.000007 0.000007 0.000030 0.000012 0.000024 0.000007
Endoplasmic Reticulum 0.003437 0.003367 0.010604 0.006130 0.004929 0.004186 0.003357 0.002099 0.004767 0.003709 0.005058 0.002470
Endosome 0.002961 0.004118 0.005119 0.001341 0.007063 0.011687 0.001125 0.003010 0.006617 0.007277 0.002062 0.002857
Golgi 0.001137 0.002203 0.000431 0.000204 0.003199 0.003764 0.000153 0.000527 0.002975 0.002855 0.000218 0.000498
Lipid Particles 0.218076* 0.079867 0.021353 0.003890 0.130541* 0.153697* 0.004430 0.015584 0.140057* 0.110683* 0.008403 0.014508
Mitochondria 0.006631 0.003873 0.001853 0.000745 0.009346 0.011574 0.000440 0.002297 0.009050 0.007088 0.000772 0.002155
Mitotic Spindle 0.010730 0.018936 0.049072 0.125605* 0.017236 0.014400 0.124345* 0.073656 0.016529 0.017043 0.106673* 0.078435
None 0.000884 0.000805 0.006205 0.000287 0.014405 0.002453 0.000126 0.000821 0.012935 0.001493 0.001553 0.000772
Nuclear Periphery 0.061061 0.041197 0.069547 0.080095 0.040594 0.049385 0.112683* 0.073794 0.042819 0.044615 0.102555* 0.074374
Nuclear Periphery Foci 0.663510* 0.824678* 0.525212* 0.726877* 0.677929* 0.700578* 0.719624* 0.795874* 0.676361* 0.772880* 0.673980* 0.789527*
Nucleolus 0.007595 0.008417 0.080617 0.023237 0.043070 0.019818 0.015425 0.014555 0.039213 0.013176 0.030731 0.015354
Nucleus 0.006299 0.005879 0.181817* 0.027821 0.027802 0.014324 0.015700 0.012389 0.025465 0.009403 0.054701 0.013809
Peroxisomes 0.008989 0.000489 0.001862 0.000454 0.002515 0.001522 0.000252 0.000266 0.003219 0.000920 0.000630 0.000283
Vacuole 0.000534 0.001242 0.008210 0.000631 0.000830 0.001675 0.000441 0.001162 0.000798 0.001423 0.002265 0.001113
Vacuole Periphery 0.000456 0.001104 0.000699 0.000214 0.000796 0.001352 0.000216 0.000742 0.000759 0.001208 0.000329 0.000693

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.07 -2.88 -1.52 -4.72 2.29 3.66 0.36 4.00 0.79 2.43 3.81 -2.36 2.95 -1.36 3.70
Bud Neck -1.05 -2.77 -5.02 -4.86 1.61 1.98 -1.79 1.09 -1.18 2.19 4.11 -3.06 -0.60 -5.40 2.93
Bud Site 1.20 -2.49 -1.22 -1.96 2.04 -0.55 1.27 0.83 0.86 -0.53 0.86 -1.72 0.42 -0.54 1.87
Cell Periphery 1.82 -3.70 1.27 -0.97 4.25 5.07 8.87 9.66 8.38 -0.00 7.39 2.00 9.72 6.15 3.76
Cytoplasm 1.32 -1.92 1.22 -0.45 2.33 4.75 8.17 6.65 2.10 -4.64 6.52 1.05 6.70 0.46 1.76
Cytoplasmic Foci 1.04 -1.73 2.82 5.35 2.46 3.74 7.79 7.14 5.46 -3.06 5.26 4.41 7.35 5.77 1.69
Eisosomes 2.46 -3.38 1.72 -2.06 3.97 6.26 10.67 11.84 7.95 0.01 9.47 0.95 11.85 5.42 3.62
Endoplasmic Reticulum 0.07 -3.68 -2.84 -3.56 2.21 1.36 2.23 6.45 6.12 2.59 2.42 -0.59 5.62 4.95 4.22
Endosome -1.00 -1.73 1.78 4.89 3.10 -2.71 6.99 4.44 5.98 -3.82 -0.40 5.34 4.56 5.77 -1.18
Golgi -1.91 1.69 2.91 4.26 2.39 -0.16 6.21 5.35 5.49 -2.57 0.80 6.20 5.51 6.12 -1.84
Lipid Particles 5.80 7.55 9.05 19.48 1.66 -2.66 20.27 18.33 20.78 -6.42 4.11 19.73 20.24 25.42 -2.02
Mitochondria 0.83 1.47 1.82 5.77 2.75 -1.16 8.81 6.39 5.57 -4.09 1.64 8.55 6.59 5.83 -3.32
Mitotic Spindle -1.02 -1.98 -6.67 -6.70 -2.45 1.64 -6.58 -12.09 -13.53 3.24 0.47 -6.72 -13.69 -14.84 2.34
None 0.58 -1.90 1.71 1.97 2.14 6.17 7.65 7.18 4.22 -4.19 6.78 6.28 7.20 2.72 1.05
Nuclear Periphery 2.31 -0.22 -1.68 -5.58 -0.93 -3.18 -7.07 -11.39 -8.15 3.70 -1.16 -6.79 -11.11 -12.45 3.04
Nuclear Periphery Foci -6.20 2.75 -1.76 5.79 -3.95 -2.72 -2.52 -11.77 -9.44 -4.13 -10.62 -0.10 -11.82 -2.00 -6.09
Nucleolus -0.06 -3.59 -3.01 -3.51 2.71 6.41 6.29 8.69 2.37 0.07 8.86 1.83 7.99 -1.24 2.50
Nucleus 0.32 -4.76 -4.00 -5.13 4.12 4.51 3.19 5.53 0.86 0.82 6.65 -2.68 4.58 -3.30 3.99
Peroxisomes 1.50 1.16 1.51 0.35 2.55 3.05 6.70 6.45 5.53 -0.69 3.77 3.98 4.65 5.04 2.05
Vacuole -1.69 -1.81 -1.09 0.35 1.70 -2.11 1.07 -0.96 1.00 -1.56 -2.19 -1.56 -1.12 0.64 1.24
Vacuole Periphery -1.84 -1.09 1.36 2.83 2.85 -1.90 4.05 0.14 1.59 -1.90 -1.88 3.16 0.20 1.57 -1.42
Standard name
Human Ortholog
Description Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
Localization
Cell Percentages nuclear periphery (73%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ulp1

Ulp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ulp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available