Standard name
Human Ortholog
Description Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway; SKS1 has a paralog, VHS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.97 0.96 0.93 0.8 0.8 0.74 0.64 0.65 0.65 0.99 0.99 1.0 0.99 1.0 – 0.98 0.95 0.94 0.94 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.18 0.22 0.2 0.35 0.43 0.42 0.4 0 0 0 0 0.07 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 3 7 1 0 3 0 0 0 0 0 – 0 1 0 0 2 0
Bud 0 1 6 0 4 1 2 3 2 2 3 3 1 2 0 0 0 – 0 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 7 4 2 7 6 12 17 18 15 14 31 1 6 0 2 1 – 0 0 0 0 0 0
Cytoplasm 161 292 241 165 354 406 418 448 310 296 181 242 291 567 641 91 41 – 154 286 227 99 288 255
Endoplasmic Reticulum 0 2 0 1 2 0 17 17 9 12 4 18 0 1 1 4 1 – 0 0 1 0 1 1
Endosome 0 0 0 1 0 0 1 1 0 0 0 0 0 0 1 0 0 – 0 2 0 1 2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – 0 1 0 0 1 2
Mitochondria 2 3 2 5 25 78 117 113 145 199 119 151 1 1 3 1 3 – 1 1 3 0 1 1
Nucleus 0 2 0 0 1 2 1 5 0 2 2 2 0 1 0 1 2 – 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 1 2 0 5 1 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 1 6 4 13 19 6 36 17 8 0 1 1 1 0 – 0 3 4 1 5 5
Unique Cell Count 162 297 241 170 369 436 522 562 417 465 280 373 293 571 644 92 41 158 302 241 106 313 281
Labelled Cell Count 163 307 253 175 399 497 582 628 498 568 341 458 295 579 647 100 48 158 302 241 106 313 281


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 7.5 6.8 6.2 6.0 5.8 5.6 6.1 6.2 6.1 6.3 6.3 7.2 6.7 6.2 7.6 8.9 – 6.5 6.8 7.0
Std Deviation (1e-4) 0.7 0.9 0.9 1.1 1.3 1.1 1.0 1.1 1.3 1.5 1.5 1.5 1.3 1.1 1.1 1.5 1.8 – 1.0 1.4 1.8
Intensity Change (Log2) – – – -0.15 -0.19 -0.24 -0.29 -0.16 -0.15 -0.17 -0.11 -0.11 0.08 -0.04 -0.14 0.14 0.39 – -0.09 -0.01 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0.6 1.1 1.8 1.2 2.1 3.5 0 0 0 0 0 –
Cytoplasm -2.7 -3.2 -4.2 -7.5 -7.5 -8.6 -10.7 -10.3 -10.4 -1.3 -1.3 -1.1 -1.6 0 –
Endoplasmic Reticulum 0 0 0 2.8 2.7 0 0 0 3.5 0 0 0 0 0 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 3.5 6.6 7.6 7.1 10.1 11.7 11.2 11.1 0 0 0 0 0 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 0 2.9 0 4.4 3.9 0 0 0 0 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5763 5.2177 4.0087 3.7411 3.5059 4.5849 3.9924 5.6209 5.328 5.1419 4.5187 5.4468 5.3429 7.237 6.5043 6.0513 6.9986 6.4983
Actin 0.0017 0.0031 0.0035 0.0015 0.0015 0.0019 0.0603 0.0012 0.0091 0.0066 0.0053 0.0107 0.1091 0.0024 0.0292 0.0109 0.0511 0.0007
Bud 0.0001 0.0069 0.0002 0.0003 0.0002 0.0003 0.0006 0.0004 0.0004 0.0004 0.0002 0.0004 0.0019 0.0002 0.0114 0.0008 0.0084 0.0002
Bud Neck 0.0002 0.0003 0.0004 0.0006 0.0005 0.0011 0.0126 0.0002 0.0009 0.0076 0.0007 0.001 0.0126 0.0002 0.0053 0.0004 0.032 0.0008
Bud Periphery 0 0.0046 0.0001 0.0001 0 0.0002 0.0007 0.0001 0.0006 0.0004 0.0001 0.0005 0.0022 0.0001 0.0095 0.0008 0.0071 0.0001
Bud Site 0.0006 0.0144 0.0018 0.0002 0.0004 0.0002 0.0133 0.002 0.0028 0.0014 0.0017 0.0002 0.0105 0.0031 0.0088 0.0008 0.0056 0.0002
Cell Periphery 0.0002 0.0007 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001 0.0002 0.0005 0.0001 0.0001 0.0007 0.0003 0.0006 0.0002 0.0002 0
Cytoplasm 0.9641 0.9495 0.956 0.9678 0.9195 0.9556 0.7261 0.9209 0.8936 0.9292 0.8641 0.9374 0.6608 0.9845 0.8551 0.8987 0.7898 0.9798
Cytoplasmic Foci 0.0034 0.0077 0.0049 0.0037 0.0367 0.0132 0.0399 0.0133 0.0249 0.0116 0.023 0.0052 0.0569 0.0029 0.0102 0.006 0.0201 0.007
Eisosomes 0 0 0 0 0.0001 0 0.0013 0 0.0001 0.0004 0.0001 0.0006 0.0005 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0132 0.0037 0.0089 0.0067 0.0019 0.0034 0.0035 0.0019 0.0031 0.0022 0.0039 0.0025 0.0094 0.0009 0.0051 0.0045 0.0017 0.0019
Endosome 0.0021 0.002 0.0046 0.0048 0.0114 0.0038 0.0216 0.0091 0.011 0.0121 0.0283 0.0071 0.0189 0.0007 0.0072 0.0191 0.0045 0.0021
Golgi 0.0004 0.0005 0.0004 0.0003 0.0009 0.0018 0.0106 0.0027 0.0067 0.0025 0.0042 0.0051 0.0145 0.0004 0.0053 0.0081 0.0055 0.0004
Lipid Particles 0.0002 0.0004 0.0003 0.0001 0.0074 0.0003 0.0063 0.0001 0.0072 0.0035 0.0175 0.0045 0.0096 0 0.0032 0.0228 0.0418 0
Mitochondria 0.0001 0.0001 0.0002 0.0002 0.0004 0.0052 0.0094 0.0116 0.0075 0.0014 0.0021 0.0113 0.0038 0.0001 0.0137 0.009 0.0026 0.0002
None 0.0071 0.0027 0.0105 0.0045 0.012 0.0083 0.0353 0.031 0.0144 0.0135 0.0114 0.0077 0.0222 0.0032 0.0157 0.0025 0.0048 0.0047
Nuclear Periphery 0.0028 0.0007 0.0027 0.0025 0.0005 0.0006 0.0073 0.0005 0.0012 0.0006 0.0031 0.0017 0.006 0.0002 0.0031 0.0047 0.0007 0.0002
Nucleolus 0 0 0 0 0.0001 0 0.0009 0 0.0003 0.0005 0.0045 0.0001 0.0029 0 0.0007 0.0002 0.0001 0
Nucleus 0.0022 0.0012 0.0017 0.0036 0.0011 0.0015 0.0047 0.0017 0.0009 0.0012 0.0041 0.0008 0.0075 0.0005 0.002 0.0012 0.0007 0.0007
Peroxisomes 0.0001 0.0001 0.0001 0 0.0019 0.0009 0.0122 0.0017 0.0082 0.0017 0.0055 0.0015 0.0272 0.0001 0.0086 0.0054 0.0207 0.0004
Punctate Nuclear 0.0004 0.0003 0.0004 0.0009 0.001 0.0003 0.0309 0.0002 0.0036 0.0006 0.0179 0.0008 0.0204 0.0001 0.0039 0.0019 0.0018 0.0002
Vacuole 0.0008 0.001 0.0025 0.0016 0.002 0.001 0.0013 0.0008 0.0025 0.0017 0.0015 0.0005 0.002 0.0002 0.0006 0.001 0.0004 0.0002
Vacuole Periphery 0.0001 0.0001 0.0004 0.0005 0.0003 0.0002 0.0005 0.0002 0.0006 0.0003 0.0005 0.0003 0.0005 0 0.0003 0.001 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.3122 11.1123 8.2458 24.1423 10.4286 8.7697 13.5488 19.8503 24.5654 16.3275
Translational Efficiency 0.9349 0.5713 0.9121 0.5023 0.5677 1.2545 0.8474 0.5433 0.6721 0.6385

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1537 1194 68 1530 968 1365 204 189 2505 2559 272 1719

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 747.42 798.70 1035.55 1209.72 794.83 842.21 1197.54 1425.33 765.74 821.91 1157.04 1233.43
Standard Deviation 93.92 117.64 151.82 198.82 96.53 114.97 141.43 234.45 97.71 118.23 160.27 213.95
Intensity Change Log 2 — 0.095735 0.470406 0.694682 — 0.083534 0.591356 0.842578 — 0.089459 0.534005 0.772794

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000076 0.000204 0.003384 0.001169 0.000169 0.000325 0.000262 0.001793 0.000112 0.000269 0.001042 0.001238
Bud Neck 0.003791 0.005487 0.002801 0.002466 0.005481 0.010241 0.001303 0.007017 0.004444 0.008023 0.001677 0.002967
Bud Site 0.005654 0.012519 0.010676 0.041368 0.007668 0.022395 0.009955 0.073885 0.006432 0.017787 0.010135 0.044944
Cell Periphery 0.000189 0.000068 0.000214 0.000130 0.000158 0.000085 0.000089 0.000546 0.000177 0.000077 0.000120 0.000176
Cytoplasm 0.728117* 0.698466* 0.696791* 0.797635* 0.637591* 0.615202* 0.720460* 0.609779* 0.693135* 0.654052* 0.714543* 0.776981*
Cytoplasmic Foci 0.108978 0.124133 0.035790 0.028655 0.197528* 0.179665 0.041452 0.084694 0.143196 0.153755 0.040036 0.034817
Eisosomes 0.000052 0.000031 0.000033 0.000008 0.000097 0.000052 0.000017 0.000019 0.000069 0.000042 0.000021 0.000009
Endoplasmic Reticulum 0.000637 0.000889 0.001828 0.000170 0.001075 0.000367 0.000564 0.001930 0.000806 0.000610 0.000880 0.000363
Endosome 0.004355 0.011709 0.009016 0.004809 0.012371 0.021963 0.003413 0.014284 0.007453 0.017178 0.004813 0.005851
Golgi 0.001178 0.003466 0.000582 0.004021 0.003740 0.005049 0.000216 0.011572 0.002168 0.004311 0.000307 0.004851
Lipid Particles 0.001677 0.001581 0.007468 0.001221 0.003696 0.002363 0.000826 0.006709 0.002457 0.001998 0.002486 0.001824
Mitochondria 0.000505 0.001666 0.000413 0.000678 0.000765 0.001562 0.000061 0.000374 0.000605 0.001611 0.000149 0.000644
Mitotic Spindle 0.000440 0.003401 0.003244 0.021890 0.002172 0.007085 0.002896 0.052443 0.001109 0.005366 0.002983 0.025249
None 0.007378 0.003001 0.001456 0.002619 0.002622 0.001396 0.002119 0.002530 0.005540 0.002145 0.001953 0.002609
Nuclear Periphery 0.000172 0.000189 0.000375 0.000123 0.000163 0.000128 0.000314 0.000148 0.000168 0.000157 0.000329 0.000126
Nuclear Periphery Foci 0.000367 0.001009 0.011054 0.001448 0.001263 0.000656 0.006581 0.004834 0.000713 0.000821 0.007699 0.001820
Nucleolus 0.000546 0.000668 0.000184 0.000168 0.000763 0.000664 0.000114 0.000160 0.000630 0.000666 0.000132 0.000167
Nucleus 0.091774 0.048352 0.103487 0.027014 0.051738 0.024993 0.041431 0.009657 0.076303 0.035892 0.056945 0.025106
Peroxisomes 0.001053 0.002543 0.003494 0.001752 0.001576 0.004081 0.000216 0.013824 0.001255 0.003364 0.001036 0.003079
Vacuole 0.042335 0.078711 0.107297 0.060863 0.068480 0.100000 0.167543 0.098721 0.052438 0.090066 0.152482 0.065025
Vacuole Periphery 0.000727 0.001905 0.000414 0.001794 0.000882 0.001728 0.000170 0.005082 0.000787 0.001811 0.000231 0.002155

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.65 -1.28 -5.81 -4.46 0.96 -2.58 -2.99 -2.76 -2.24 -2.31 -4.78 -1.57 -6.13 -4.40 0.27
Bud Neck -2.59 1.16 4.58 6.06 0.45 -4.44 6.19 1.17 5.01 -3.42 -5.73 6.19 5.99 10.39 -1.60
Bud Site -4.09 -2.07 -6.67 -3.53 -2.51 -6.88 -1.24 -3.73 -1.36 -3.46 -8.17 -2.87 -7.52 -1.89 -5.49
Cell Periphery 1.89 -0.38 1.25 -4.14 2.72 8.91 6.00 -2.02 -3.05 -2.89 2.55 1.26 1.15 -4.49 -0.28
Cytoplasm 3.59 3.00 -1.04 -4.29 -3.25 2.20 1.96 0.59 -0.48 -0.94 5.88 5.23 -4.12 -8.96 -6.93
Cytoplasmic Foci -2.74 11.89 22.52 20.91 1.30 2.57 24.89 16.90 15.29 -3.38 -2.19 25.59 32.94 33.16 1.81
Eisosomes 7.83 1.17 19.79 14.45 4.45 7.69 13.76 14.17 11.54 1.84 9.32 12.84 23.64 21.48 7.11
Endoplasmic Reticulum -1.15 -4.60 6.82 3.81 6.13 3.06 1.78 1.60 -0.47 0.53 1.48 -1.68 6.06 4.39 7.67
Endosome -7.17 -1.60 4.08 9.17 2.47 -6.16 9.47 5.19 10.03 -0.95 -10.69 3.89 8.99 16.79 2.67
Golgi -2.71 2.22 -0.30 2.17 -1.56 -1.25 4.48 0.68 1.42 -1.39 -3.46 5.35 1.09 3.91 -2.37
Lipid Particles 0.44 -0.90 -1.24 -1.72 0.82 1.89 6.97 -2.42 -3.17 -4.33 1.22 0.22 -0.38 -1.39 -0.32
Mitochondria -2.79 0.87 1.78 3.36 0.19 -2.78 5.06 4.57 5.85 -0.65 -4.13 5.25 2.96 5.48 -1.06
Mitotic Spindle -2.95 -1.48 -6.41 -4.27 -4.51 -2.45 -1.14 -2.41 -1.64 -2.15 -3.84 -2.42 -6.44 -3.69 -4.73
None 4.01 5.78 5.16 2.32 -1.93 3.26 1.63 1.01 -0.59 -0.09 5.04 5.43 5.30 0.79 -0.65
Nuclear Periphery -0.82 -5.16 -3.43 -1.51 4.17 1.01 -6.86 -2.33 -2.99 5.17 0.50 -8.29 -4.03 -3.55 6.85
Nuclear Periphery Foci -5.57 -5.66 -11.26 -5.87 4.77 3.48 -6.87 -5.82 -6.91 1.21 -1.29 -9.24 -10.44 -9.78 6.79
Nucleolus -0.50 1.77 1.87 5.62 0.23 0.34 2.39 2.44 2.86 0.65 -0.11 3.09 2.86 4.44 -2.01
Nucleus 12.07 -1.09 18.06 5.52 5.36 11.63 -0.21 13.55 4.57 6.91 17.64 1.82 18.87 1.82 6.20
Peroxisomes -2.87 -0.65 0.28 3.51 0.71 -5.20 5.34 -1.18 0.14 -1.92 -5.66 0.86 0.01 5.11 -0.82
Vacuole -9.89 -5.22 -20.05 -11.38 0.76 -7.20 -11.56 -9.22 -7.02 2.69 -13.17 -13.15 -20.77 -12.07 5.94
Vacuole Periphery -3.45 2.14 0.47 3.61 -1.27 -4.90 9.60 -0.31 1.00 -1.52 -5.39 6.21 0.61 4.78 -2.78
Standard name
Human Ortholog
Description Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway; SKS1 has a paralog, VHS1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sks1

Sks1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sks1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available