Standard name | |
---|---|
Human Ortholog | |
Description | ATP-dependent RNA helicase; component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron; expression of a processed form of human homolog SUPV3L1 carrying an N-terminal deletion of 46 amino acids rescues yeast suv3 null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 9 | 11 | 12 | 0 | 2 | 3 | 2 | – | 2 | 5 | 6 | 1 | 2 | 4 | 3 | 6 | 1 | 1 | 1 | 2 | 22 | 30 |
Bud | 5 | 1 | 15 | 10 | 10 | 3 | 21 | 19 | 20 | – | 12 | 19 | 17 | 15 | 12 | 4 | 3 | 4 | 0 | 0 | 1 | 1 | 5 | 5 |
Bud Neck | 0 | 0 | 2 | 1 | 3 | 0 | 3 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 5 | 13 |
Bud Site | 1 | 0 | 0 | 2 | 0 | 2 | 7 | 3 | 12 | – | 5 | 10 | 2 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 7 | 4 | 5 | 2 | 2 | 0 | 1 | – | 2 | 4 | 17 | 16 | 6 | 10 | 11 | 4 | 0 | 0 | 1 | 1 | 2 | 3 |
Cytoplasm | 3 | 1 | 15 | 10 | 14 | 14 | 25 | 26 | 10 | – | 15 | 9 | 9 | 24 | 37 | 4 | 11 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 9 | 4 | 5 | 1 | 4 | 3 | 3 | 7 | – | 4 | 6 | 8 | 5 | 20 | 1 | 4 | 6 | 0 | 0 | 0 | 3 | 6 | 6 |
Endosome | 3 | 1 | 18 | 1 | 24 | 2 | 7 | 21 | 2 | – | 0 | 1 | 21 | 52 | 58 | 9 | 10 | 5 | 12 | 8 | 28 | 18 | 50 | 61 |
Golgi | 0 | 6 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 6 | 7 | 20 | 28 | 17 | 2 | 4 | 3 | 4 | 22 | 33 |
Mitochondria | 144 | 120 | 186 | 165 | 173 | 245 | 409 | 366 | 397 | – | 285 | 294 | 422 | 411 | 471 | 9 | 14 | 11 | 111 | 106 | 227 | 153 | 379 | 513 |
Nucleus | 0 | 0 | 4 | 1 | 5 | 12 | 23 | 30 | 4 | – | 7 | 10 | 2 | 1 | 0 | 2 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 3 |
Nuclear Periphery | 12 | 5 | 0 | 1 | 2 | 4 | 6 | 3 | 10 | – | 8 | 4 | 5 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 4 | 8 |
Nucleolus | 5 | 0 | 0 | 0 | 2 | 4 | 6 | 6 | 7 | – | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 3 |
Peroxisomes | 1 | 1 | 16 | 2 | 10 | 2 | 13 | 8 | 3 | – | 0 | 2 | 29 | 58 | 32 | 2 | 10 | 2 | 11 | 3 | 8 | 23 | 50 | 62 |
SpindlePole | 2 | 1 | 23 | 8 | 39 | 16 | 41 | 79 | 31 | – | 10 | 22 | 8 | 10 | 9 | 6 | 14 | 9 | 1 | 0 | 2 | 1 | 9 | 6 |
Vac/Vac Membrane | 5 | 14 | 28 | 9 | 30 | 15 | 25 | 17 | 21 | – | 9 | 12 | 30 | 72 | 57 | 9 | 24 | 14 | 1 | 3 | 8 | 8 | 16 | 24 |
Unique Cell Count | 149 | 127 | 237 | 187 | 255 | 274 | 478 | 496 | 420 | 305 | 326 | 459 | 528 | 595 | 70 | 108 | 68 | 146 | 131 | 296 | 229 | 584 | 790 | |
Labelled Cell Count | 186 | 161 | 327 | 232 | 332 | 325 | 593 | 586 | 527 | 361 | 400 | 580 | 673 | 714 | 80 | 132 | 88 | 146 | 131 | 296 | 229 | 584 | 790 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.0 | 5.3 | 4.8 | 5.5 | 4.3 | 4.4 | 4.8 | 3.8 | – | 3.7 | 4.2 | 5.0 | 5.1 | 5.2 | 6.9 | 7.6 | 7.3 | 4.7 | 4.9 | 5.2 |
Std Deviation (1e-4) | 0.7 | 0.7 | 0.9 | 0.9 | 1.0 | 0.9 | 1.1 | 1.1 | 0.7 | – | 1.1 | 0.7 | 0.9 | 1.0 | 1.0 | 1.2 | 2.2 | 1.4 | 1.0 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.03 | -0.3 | -0.28 | -0.15 | -0.48 | – | -0.54 | -0.34 | -0.1 | -0.06 | -0.05 | 0.37 | 0.5 | 0.45 | -0.19 | -0.13 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0.5 | 0 | 0 | 0 | 0 | – | 0 | 0 | -2.1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -1.2 | 0 | 0 | 0 | 0 | – | 0 | 0 | -1.6 | -2.3 | -3.2 | 0 | -1.4 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.5 | 0.1 | 0 | 0 | 2.8 | 0 |
Cytoplasm | -0.4 | -0.4 | -0.6 | -0.6 | -0.6 | -2.5 | – | -0.7 | -2.1 | -3.0 | -1.0 | -0.1 | 0 | 1.3 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 |
Endosome | 0 | 0.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | -1.6 | 1.0 | 1.0 | 1.4 | 0.5 | -0.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 8.2 | 0 |
Mitochondria | 0 | -2.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 5.1 | -0.2 | 0.2 | -10.0 | -11.4 | -9.5 |
Nucleus | 0 | 0 | 1.7 | 2.1 | 2.6 | 0 | – | 0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | -1.4 | 0 | 0 | 0 | 0 | – | 0 | 0 | -0.2 | 1.8 | -0.8 | 0 | 0.8 | 0 |
SpindlePole | 0 | 1.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | -4.8 | -4.9 | -5.5 | -0.3 | 0.9 | 0.8 |
Vacuole | -2.5 | 0 | -2.6 | -3.2 | -4.4 | -3.2 | – | -4.1 | -3.7 | -2.4 | 0.7 | -1.0 | 0.2 | 2.5 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2872 | 0.105 | -0.3446 | -0.4605 | -0.606 | -0.1344 | 0.2854 | 0.1487 | -0.002 | -0.1507 | 0.5462 | 0.0695 | 0.2724 | 0.4934 | 0.089 | 0.0629 | 0.0443 | 0.1759 |
Actin | 0.0224 | 0.0285 | 0.0242 | 0.0022 | 0.0129 | 0.0115 | 0.0474 | 0.0213 | 0.033 | 0.0013 | 0.0148 | 0.0151 | 0.0436 | 0.016 | 0.0351 | 0.0018 | 0.0042 | 0.0218 |
Bud | 0.0009 | 0.0023 | 0.0061 | 0.001 | 0.0005 | 0.0011 | 0.0011 | 0.0031 | 0.0014 | 0.0014 | 0.0005 | 0.0007 | 0.0006 | 0.0017 | 0.0021 | 0.0006 | 0.0005 | 0.0009 |
Bud Neck | 0.0016 | 0.0036 | 0.001 | 0.0015 | 0.0004 | 0.0018 | 0.0014 | 0.0011 | 0.0007 | 0.0002 | 0.0009 | 0.0022 | 0.001 | 0.0007 | 0.0024 | 0.0006 | 0.0005 | 0.0184 |
Bud Periphery | 0.0016 | 0.0025 | 0.0033 | 0.0042 | 0.0013 | 0.0037 | 0.0024 | 0.009 | 0.002 | 0.0148 | 0.0011 | 0.0022 | 0.001 | 0.0041 | 0.0041 | 0.0018 | 0.0015 | 0.003 |
Bud Site | 0.0127 | 0.0185 | 0.0074 | 0.0015 | 0.0012 | 0.0019 | 0.006 | 0.022 | 0.004 | 0.0005 | 0.0014 | 0.001 | 0.0019 | 0.0148 | 0.0104 | 0.0004 | 0.0006 | 0.0015 |
Cell Periphery | 0.0008 | 0.0006 | 0.0008 | 0.0015 | 0.0003 | 0.0005 | 0.0014 | 0.0033 | 0.0005 | 0.0016 | 0.0002 | 0.0008 | 0.0004 | 0.0017 | 0.0012 | 0.0003 | 0.0004 | 0.0034 |
Cytoplasm | 0.0105 | 0.0041 | 0.0059 | 0.0048 | 0.0013 | 0.0104 | 0.0077 | 0.0088 | 0.0022 | 0.0024 | 0.0235 | 0.0106 | 0.0067 | 0.0153 | 0.0066 | 0.0013 | 0.0037 | 0.0248 |
Cytoplasmic Foci | 0.0803 | 0.0791 | 0.048 | 0.071 | 0.0452 | 0.0534 | 0.0572 | 0.0703 | 0.034 | 0.017 | 0.0658 | 0.0367 | 0.067 | 0.0761 | 0.0479 | 0.0332 | 0.0319 | 0.0604 |
Eisosomes | 0.0008 | 0.0009 | 0.0029 | 0.0003 | 0.0005 | 0.0002 | 0.001 | 0.0004 | 0.0008 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0008 | 0.0007 | 0.0001 | 0.0003 | 0.0004 |
Endoplasmic Reticulum | 0.0048 | 0.0009 | 0.0015 | 0.0011 | 0.0003 | 0.0009 | 0.0045 | 0.0006 | 0.0008 | 0.0009 | 0.003 | 0.0006 | 0.008 | 0.0049 | 0.001 | 0.0002 | 0.0005 | 0.0011 |
Endosome | 0.0999 | 0.0991 | 0.072 | 0.0603 | 0.1226 | 0.0497 | 0.1332 | 0.0695 | 0.0763 | 0.0451 | 0.1544 | 0.0709 | 0.1199 | 0.0895 | 0.0581 | 0.0408 | 0.1179 | 0.0905 |
Golgi | 0.0848 | 0.1051 | 0.0517 | 0.021 | 0.0652 | 0.0619 | 0.0883 | 0.0854 | 0.0691 | 0.0401 | 0.1254 | 0.0835 | 0.0907 | 0.082 | 0.0587 | 0.0491 | 0.1123 | 0.0601 |
Lipid Particles | 0.0918 | 0.0563 | 0.0444 | 0.0392 | 0.0507 | 0.042 | 0.0494 | 0.0112 | 0.017 | 0.0024 | 0.0761 | 0.0286 | 0.0859 | 0.0197 | 0.0243 | 0.0321 | 0.0482 | 0.0347 |
Mitochondria | 0.4495 | 0.4734 | 0.6094 | 0.6722 | 0.6113 | 0.6673 | 0.4968 | 0.6191 | 0.7033 | 0.8475 | 0.3825 | 0.6879 | 0.4246 | 0.566 | 0.6694 | 0.7836 | 0.5368 | 0.6022 |
None | 0.0245 | 0.0214 | 0.0293 | 0.0021 | 0.0007 | 0.0025 | 0.0093 | 0.0031 | 0.0083 | 0.0008 | 0.0263 | 0.0171 | 0.0231 | 0.0378 | 0.0016 | 0.0002 | 0.0272 | 0.0169 |
Nuclear Periphery | 0.0072 | 0.0003 | 0.0018 | 0.0003 | 0.0001 | 0.0022 | 0.0188 | 0.0002 | 0.0004 | 0.0002 | 0.0155 | 0.0002 | 0.0132 | 0.0005 | 0.0006 | 0.0001 | 0.0006 | 0.0012 |
Nucleolus | 0.0009 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0005 | 0.0019 | 0.0006 | 0.0002 | 0.0001 | 0.0009 | 0.0003 | 0.0012 | 0.0008 | 0.0006 | 0.0002 | 0.0014 | 0.001 |
Nucleus | 0.0026 | 0.0004 | 0.0006 | 0.0006 | 0.0001 | 0.001 | 0.0195 | 0.0005 | 0.0002 | 0.0002 | 0.0022 | 0.0004 | 0.0045 | 0.0008 | 0.0005 | 0.0001 | 0.0007 | 0.0007 |
Peroxisomes | 0.0784 | 0.0964 | 0.08 | 0.0951 | 0.0809 | 0.0802 | 0.0248 | 0.0634 | 0.0401 | 0.0178 | 0.0848 | 0.0298 | 0.0787 | 0.0552 | 0.066 | 0.0479 | 0.0652 | 0.0452 |
Punctate Nuclear | 0.0065 | 0.0006 | 0.0007 | 0.0006 | 0.0002 | 0.0018 | 0.0137 | 0.0005 | 0.0003 | 0 | 0.0123 | 0.001 | 0.017 | 0.0004 | 0.0017 | 0.0001 | 0.0004 | 0.0013 |
Vacuole | 0.0084 | 0.0032 | 0.005 | 0.0131 | 0.0023 | 0.0032 | 0.008 | 0.0045 | 0.0026 | 0.0027 | 0.004 | 0.0063 | 0.0057 | 0.0076 | 0.0043 | 0.0025 | 0.0271 | 0.0058 |
Vacuole Periphery | 0.0091 | 0.0025 | 0.0032 | 0.0059 | 0.0019 | 0.0024 | 0.006 | 0.0023 | 0.0029 | 0.0029 | 0.004 | 0.0037 | 0.0046 | 0.0035 | 0.0026 | 0.003 | 0.0181 | 0.0047 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.9708 | 7.1283 | 7.6036 | 10.1639 | 8.7232 | 7.4715 | 10.6985 | 11.0972 | 10.395 | 13.725 |
Translational Efficiency | 0.43 | 0.509 | 0.5002 | 0.3771 | 0.3535 | 0.5188 | 0.5981 | 0.3894 | 0.4906 | 0.3877 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
767 | 1136 | 792 | 1105 | 1512 | 939 | 1731 | 3008 | 2279 | 2075 | 2523 | 4113 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 632.60 | 767.75 | 825.12 | 867.70 | 697.84 | 715.61 | 816.10 | 905.15 | 675.88 | 744.16 | 818.93 | 895.09 |
Standard Deviation | 75.21 | 104.92 | 176.13 | 124.15 | 110.41 | 134.08 | 104.11 | 117.39 | 104.61 | 121.80 | 131.12 | 120.39 |
Intensity Change Log 2 | — | 0.279343 | 0.383310 | 0.455903 | — | 0.036277 | 0.225850 | 0.375261 | — | 0.156965 | 0.302865 | 0.414167 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000507 | 0.001694 | 0.006344 | 0.024161 | 0.001550 | 0.001082 | 0.006110 | 0.008443 | 0.001199 | 0.001417 | 0.006184 | 0.012666 |
Bud Neck | 0.013305 | 0.004334 | 0.010710 | 0.011873 | 0.006824 | 0.012384 | 0.042905 | 0.002360 | 0.009005 | 0.007977 | 0.032798 | 0.004916 |
Bud Site | 0.012032 | 0.007310 | 0.041293 | 0.078115 | 0.009107 | 0.007577 | 0.030772 | 0.032289 | 0.010091 | 0.007431 | 0.034074 | 0.044600 |
Cell Periphery | 0.000586 | 0.000298 | 0.000325 | 0.001033 | 0.000479 | 0.000504 | 0.001915 | 0.000247 | 0.000515 | 0.000391 | 0.001416 | 0.000458 |
Cytoplasm | 0.011569 | 0.002935 | 0.009449 | 0.016539 | 0.004727 | 0.007277 | 0.035595 | 0.014562 | 0.007030 | 0.004900 | 0.027387 | 0.015093 |
Cytoplasmic Foci | 0.061973 | 0.062930 | 0.097495 | 0.078385 | 0.049798 | 0.062207 | 0.104903 | 0.057683 | 0.053896 | 0.062603 | 0.102578 | 0.063245 |
Eisosomes | 0.000135 | 0.000089 | 0.000118 | 0.000186 | 0.000081 | 0.000305 | 0.000540 | 0.000109 | 0.000099 | 0.000186 | 0.000408 | 0.000129 |
Endoplasmic Reticulum | 0.000674 | 0.000207 | 0.003112 | 0.002604 | 0.000363 | 0.000399 | 0.001939 | 0.000545 | 0.000468 | 0.000294 | 0.002307 | 0.001098 |
Endosome | 0.063791 | 0.095628 | 0.126839 | 0.104425 | 0.084543 | 0.091604 | 0.118851 | 0.065056 | 0.077559 | 0.093807 | 0.121358 | 0.075633 |
Golgi | 0.099824 | 0.135246* | 0.109399 | 0.116687* | 0.113767* | 0.137731* | 0.085092 | 0.143298* | 0.109074 | 0.136370* | 0.092722 | 0.136149* |
Lipid Particles | 0.014847 | 0.004627 | 0.005661 | 0.006304 | 0.007707 | 0.010231 | 0.032118 | 0.000258 | 0.010110 | 0.007163 | 0.023813 | 0.001883 |
Mitochondria | 0.621274* | 0.574697* | 0.276457* | 0.352474* | 0.640083* | 0.582409* | 0.315449* | 0.558869* | 0.633753* | 0.578186* | 0.303209* | 0.503419* |
Mitotic Spindle | 0.006891 | 0.014539 | 0.141825* | 0.048582 | 0.006086 | 0.009830 | 0.043570 | 0.037927 | 0.006357 | 0.012408 | 0.074413 | 0.040790 |
None | 0.001380 | 0.000235 | 0.001708 | 0.000551 | 0.000774 | 0.002723 | 0.006855 | 0.000249 | 0.000978 | 0.001361 | 0.005239 | 0.000330 |
Nuclear Periphery | 0.000198 | 0.000042 | 0.000828 | 0.001122 | 0.000088 | 0.000195 | 0.000789 | 0.000074 | 0.000125 | 0.000111 | 0.000801 | 0.000355 |
Nuclear Periphery Foci | 0.000423 | 0.000108 | 0.002337 | 0.001028 | 0.000326 | 0.000682 | 0.001426 | 0.000092 | 0.000359 | 0.000368 | 0.001712 | 0.000343 |
Nucleolus | 0.002710 | 0.000539 | 0.006656 | 0.001459 | 0.000574 | 0.002342 | 0.007766 | 0.000048 | 0.001293 | 0.001355 | 0.007418 | 0.000427 |
Nucleus | 0.004131 | 0.000521 | 0.010902 | 0.004513 | 0.000690 | 0.004327 | 0.026858 | 0.001430 | 0.001848 | 0.002243 | 0.021849 | 0.002259 |
Peroxisomes | 0.055227 | 0.078686 | 0.061911 | 0.082119 | 0.052514 | 0.046176 | 0.074325 | 0.037606 | 0.053427 | 0.063974 | 0.070428 | 0.049565 |
Vacuole | 0.011185 | 0.006395 | 0.057570 | 0.048461 | 0.008037 | 0.008593 | 0.047160 | 0.025754 | 0.009097 | 0.007390 | 0.050428 | 0.031855 |
Vacuole Periphery | 0.017338 | 0.008940 | 0.029062 | 0.019377 | 0.011882 | 0.011424 | 0.015062 | 0.013101 | 0.013718 | 0.010064 | 0.019457 | 0.014787 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.58 | -5.66 | -8.52 | -8.06 | -5.98 | 0.76 | -4.14 | -7.33 | -9.10 | -1.96 | -0.53 | -6.13 | -11.40 | -11.80 | -5.47 |
Bud Neck | 3.32 | 0.78 | 0.41 | -4.96 | -0.58 | -2.33 | -11.09 | 3.66 | 4.72 | 13.32 | 0.64 | -9.80 | 3.22 | 2.60 | 12.81 |
Bud Site | 2.10 | -7.01 | -12.93 | -14.87 | -6.17 | 1.00 | -9.31 | -12.71 | -13.15 | -0.79 | 2.22 | -11.56 | -17.62 | -20.21 | -4.33 |
Cell Periphery | 2.45 | 2.21 | -2.12 | -4.14 | -3.98 | -0.25 | -4.34 | 3.68 | 3.08 | 5.13 | 1.81 | -3.89 | 0.76 | -1.08 | 4.17 |
Cytoplasm | 4.45 | 0.69 | -2.53 | -9.68 | -3.82 | -1.64 | -11.40 | -8.37 | -4.63 | 7.47 | 1.89 | -10.21 | -7.70 | -10.29 | 5.95 |
Cytoplasmic Foci | -0.09 | -6.53 | -3.34 | -3.80 | 3.71 | -3.06 | -13.16 | -2.51 | 1.43 | 12.46 | -2.90 | -14.57 | -3.55 | -0.16 | 13.03 |
Eisosomes | 1.97 | 0.57 | -2.03 | -7.87 | -5.04 | -1.53 | -4.43 | -5.24 | 1.35 | 4.16 | -1.31 | -4.32 | -3.33 | 0.86 | 3.91 |
Endoplasmic Reticulum | 3.19 | -6.44 | -3.49 | -4.49 | 1.05 | -0.42 | -4.07 | -1.81 | -1.34 | 3.44 | 2.59 | -6.42 | -3.76 | -4.93 | 3.76 |
Endosome | -5.40 | -8.94 | -6.76 | -1.37 | 3.07 | -1.25 | -6.30 | 4.92 | 5.67 | 12.19 | -3.97 | -10.14 | 0.65 | 5.19 | 12.17 |
Golgi | -4.45 | -0.99 | -2.01 | 2.66 | -1.05 | -3.16 | 5.34 | -5.15 | -0.75 | -12.24 | -5.06 | 3.90 | -5.77 | 0.18 | -10.90 |
Lipid Particles | 4.40 | 4.06 | 3.68 | -1.19 | -0.40 | -1.10 | -9.17 | 6.33 | 4.99 | 13.14 | 1.96 | -7.03 | 7.58 | 4.63 | 12.75 |
Mitochondria | 2.73 | 20.70 | 16.44 | 14.94 | -5.06 | 3.89 | 27.07 | 7.39 | 1.78 | -24.27* | 5.07 | 33.92 | 14.13 | 7.80 | -23.70* |
Mitotic Spindle | -3.75 | -16.00 | -10.59 | -8.30 | 10.00 | -2.36 | -13.03 | -16.25 | -11.77 | 1.51 | -4.77 | -19.87 | -19.25 | -13.89 | 8.67 |
None | 3.53 | -0.46 | 2.35 | -2.37 | 1.71 | -2.00 | -5.18 | 2.10 | 2.58 | 5.73 | -0.86 | -5.09 | 3.19 | 2.35 | 5.98 |
Nuclear Periphery | 2.72 | -3.99 | -2.20 | -2.59 | -0.52 | -1.16 | -7.10 | 0.54 | 1.25 | 7.21 | 0.29 | -7.97 | -2.06 | -2.08 | 3.24 |
Nuclear Periphery Foci | 2.72 | -3.87 | -2.48 | -4.14 | 2.48 | -1.15 | -3.38 | 2.31 | 2.11 | 4.20 | -0.00 | -4.93 | 0.13 | 0.10 | 5.04 |
Nucleolus | 2.40 | -2.50 | 1.45 | -1.46 | 3.39 | -2.61 | -8.70 | 2.60 | 3.40 | 9.57 | -0.60 | -7.97 | 2.85 | 2.75 | 9.50 |
Nucleus | 3.18 | -3.52 | -0.40 | -4.40 | 3.39 | -3.33 | -11.98 | -2.13 | 2.05 | 11.13 | -0.71 | -12.14 | -1.33 | -0.51 | 11.54 |
Peroxisomes | -3.44 | -1.01 | -3.92 | -0.49 | -2.88 | 1.27 | -4.50 | 3.81 | 2.22 | 9.09 | -2.57 | -4.33 | 1.16 | 3.92 | 6.02 |
Vacuole | 3.19 | -11.95 | -11.20 | -13.41 | 1.91 | -0.47 | -14.43 | -12.40 | -11.27 | 7.15 | 2.05 | -18.57 | -16.33 | -18.16 | 7.32 |
Vacuole Periphery | 3.94 | -3.95 | -0.81 | -5.83 | 3.60 | 0.31 | -2.21 | -1.13 | -1.21 | 1.48 | 3.13 | -4.19 | -0.98 | -4.75 | 3.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|