Standard name | |
---|---|
Human Ortholog | |
Description | Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 1 | – | 4 | 7 | 12 | 5 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 2 | 0 | 0 | 1 | – | 3 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 0 | 0 | 2 | 1 | – | 9 | 1 | 14 | 4 | 4 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 570 | 207 | 179 | 177 | 202 | 158 | – | 187 | 134 | 184 | 139 | 179 | 119 | 200 | 180 | 192 | 307 | 195 | 559 | 197 | 161 | 72 | 162 | 160 |
Endoplasmic Reticulum | 2 | 1 | 0 | 0 | 6 | 15 | – | 64 | 32 | 79 | 33 | 74 | 1 | 0 | 2 | 5 | 7 | 16 | 3 | 3 | 7 | 1 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | – | 14 | 16 | 14 | 6 | 21 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 0 | 0 | 0 | 2 | 0 | 0 | – | 3 | 5 | 9 | 23 | 34 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 4 | 1 | 1 | 2 | 6 | 5 |
Unique Cell Count | 573 | 209 | 180 | 178 | 209 | 175 | 275 | 179 | 292 | 200 | 300 | 120 | 200 | 183 | 194 | 311 | 199 | 579 | 209 | 177 | 81 | 181 | 189 | |
Labelled Cell Count | 576 | 211 | 181 | 179 | 211 | 178 | 288 | 196 | 315 | 213 | 333 | 121 | 201 | 184 | 199 | 319 | 216 | 579 | 209 | 177 | 81 | 181 | 189 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 133.4 | 170.5 | 82.9 | 107.8 | 94.1 | 78.1 | – | 66.3 | 63.9 | 62.9 | 57.7 | 57.1 | 134.0 | 136.7 | 127.9 | 161.1 | 140.1 | 153.6 | 129.0 | 139.0 | 135.3 |
Std Deviation (1e-4) | 26.0 | 31.3 | 24.4 | 23.8 | 21.9 | 18.7 | – | 16.2 | 15.5 | 14.8 | 14.1 | 12.8 | 39.7 | 35.8 | 31.8 | 56.0 | 51.9 | 46.5 | 36.8 | 48.8 | 50.7 |
Intensity Change (Log2) | – | – | – | 0.38 | 0.18 | -0.09 | – | -0.32 | -0.38 | -0.4 | -0.52 | -0.54 | 0.69 | 0.72 | 0.62 | 0.96 | 0.76 | 0.89 | 0.64 | 0.75 | 0.71 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -1.9 | -3.9 | – | -8.3 | -7.0 | -9.1 | -7.9 | -9.7 | -0.3 | 1.1 | -1.0 | -0.5 | -0.8 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | 4.0 | – | 7.0 | 5.9 | 7.6 | 5.7 | 7.2 | 0 | 0 | 0 | 0 | 0 | 3.9 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 3.1 | 4.1 | 3.0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.7 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 117.455 | 146.9669 | 135.3462 | 129.6613 | 124.228 | 133.5966 | 102.0603 | 144.0617 | 127.8778 | 126.9753 | 98.4946 | 115.3738 | 120.9542 | 156.4469 | 141.0917 | 134.9568 | 134.1645 | 127.3908 |
Actin | 0.0012 | 0.0002 | 0.0029 | 0.0004 | 0.0012 | 0.0019 | 0.0051 | 0.0002 | 0.0007 | 0.0008 | 0.0009 | 0.0046 | 0.0028 | 0.0003 | 0.0058 | 0.0011 | 0.0077 | 0.0009 |
Bud | 0.0012 | 0.0007 | 0.0012 | 0.0016 | 0.0013 | 0.0015 | 0.0016 | 0.0005 | 0.0014 | 0.0032 | 0.0009 | 0.0014 | 0.0011 | 0.0003 | 0.0007 | 0.0009 | 0.0011 | 0.0005 |
Bud Neck | 0.0005 | 0.0005 | 0.0006 | 0.0006 | 0.0008 | 0.002 | 0.0006 | 0.0003 | 0.0006 | 0.0012 | 0.0006 | 0.0013 | 0.0005 | 0.0004 | 0.0008 | 0.0005 | 0.0007 | 0.0015 |
Bud Periphery | 0.0014 | 0.0004 | 0.0008 | 0.001 | 0.0007 | 0.0019 | 0.0016 | 0.0002 | 0.0007 | 0.0035 | 0.0008 | 0.0013 | 0.001 | 0.0001 | 0.0005 | 0.0003 | 0.0011 | 0.0005 |
Bud Site | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.001 | 0.0003 | 0.0005 | 0.0004 | 0.0063 | 0.0008 | 0.0006 | 0.0002 | 0.0003 | 0.0005 | 0.0012 | 0.0002 |
Cell Periphery | 0.0199 | 0.0081 | 0.0121 | 0.01 | 0.0038 | 0.0087 | 0.0133 | 0.0067 | 0.0095 | 0.0073 | 0.0016 | 0.0014 | 0.0175 | 0.005 | 0.0073 | 0.0025 | 0.0103 | 0.0071 |
Cytoplasm | 0.7255 | 0.8982 | 0.8229 | 0.6417 | 0.7947 | 0.8292 | 0.6901 | 0.9598 | 0.8194 | 0.704 | 0.643 | 0.7351 | 0.7315 | 0.945 | 0.8628 | 0.6808 | 0.7736 | 0.8427 |
Cytoplasmic Foci | 0.002 | 0.0024 | 0.004 | 0.0034 | 0.0054 | 0.006 | 0.0233 | 0.0014 | 0.0054 | 0.0064 | 0.0291 | 0.0063 | 0.0113 | 0.0011 | 0.0057 | 0.0046 | 0.0088 | 0.0087 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 |
Endoplasmic Reticulum | 0.0558 | 0.0079 | 0.0189 | 0.0025 | 0.0105 | 0.0445 | 0.0415 | 0.0026 | 0.0166 | 0.0149 | 0.0035 | 0.0097 | 0.0374 | 0.0048 | 0.0103 | 0.0025 | 0.0042 | 0.0199 |
Endosome | 0.012 | 0.0057 | 0.0096 | 0.0186 | 0.03 | 0.0196 | 0.0145 | 0.0022 | 0.0084 | 0.0121 | 0.025 | 0.0402 | 0.0119 | 0.0023 | 0.0084 | 0.0172 | 0.0161 | 0.0291 |
Golgi | 0.0042 | 0.0014 | 0.0036 | 0.0012 | 0.0077 | 0.0073 | 0.0035 | 0.0004 | 0.0013 | 0.0005 | 0.0032 | 0.0264 | 0.0056 | 0.0003 | 0.0016 | 0.0015 | 0.0032 | 0.0031 |
Lipid Particles | 0.0038 | 0.0007 | 0.002 | 0.0012 | 0.0005 | 0.0013 | 0.0093 | 0.0012 | 0.0041 | 0.0026 | 0.0169 | 0.0021 | 0.0069 | 0.0004 | 0.002 | 0.0032 | 0.0104 | 0.0032 |
Mitochondria | 0.0076 | 0.0026 | 0.0029 | 0.001 | 0.0076 | 0.004 | 0.0188 | 0.0002 | 0.0081 | 0.0112 | 0.035 | 0.0124 | 0.0085 | 0.0002 | 0.0067 | 0.0194 | 0.0085 | 0.0011 |
None | 0.0017 | 0.0007 | 0.0009 | 0.0004 | 0.0004 | 0.0009 | 0.0068 | 0.0001 | 0.0054 | 0.0045 | 0.0044 | 0.0021 | 0.0043 | 0.0001 | 0.0012 | 0.0017 | 0.0031 | 0.0004 |
Nuclear Periphery | 0.0192 | 0.0025 | 0.0058 | 0.0077 | 0.0071 | 0.0054 | 0.0243 | 0.0015 | 0.0062 | 0.0058 | 0.0095 | 0.0318 | 0.0193 | 0.0026 | 0.0047 | 0.0053 | 0.007 | 0.0081 |
Nucleolus | 0.0043 | 0.0008 | 0.0025 | 0.0012 | 0.0004 | 0.0029 | 0.0105 | 0.0001 | 0.0063 | 0.0079 | 0.0206 | 0.0012 | 0.0063 | 0.0001 | 0.0034 | 0.0049 | 0.0119 | 0.0003 |
Nucleus | 0.005 | 0.0019 | 0.0028 | 0.005 | 0.0045 | 0.0074 | 0.0138 | 0.0011 | 0.0048 | 0.0037 | 0.0089 | 0.0122 | 0.0068 | 0.0013 | 0.0029 | 0.0064 | 0.0075 | 0.0026 |
Peroxisomes | 0.0004 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0042 | 0 | 0.0009 | 0.0009 | 0.0073 | 0.0006 | 0.0099 | 0 | 0.0004 | 0.0053 | 0.0042 | 0.0001 |
Punctate Nuclear | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0043 | 0 | 0.0065 | 0.0001 | 0.0137 | 0.0028 | 0.0027 | 0 | 0.0002 | 0.0004 | 0.004 | 0.0001 |
Vacuole | 0.1072 | 0.0561 | 0.0842 | 0.2315 | 0.0886 | 0.0365 | 0.0822 | 0.019 | 0.0778 | 0.1509 | 0.1015 | 0.0709 | 0.0844 | 0.0319 | 0.061 | 0.1842 | 0.0843 | 0.0463 |
Vacuole Periphery | 0.0265 | 0.0085 | 0.0205 | 0.0703 | 0.0343 | 0.0184 | 0.029 | 0.0022 | 0.0151 | 0.0577 | 0.0671 | 0.0354 | 0.0291 | 0.0035 | 0.013 | 0.0567 | 0.0304 | 0.0238 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 712.3244 | 655.5032 | 622.298 | 703.8521 | 707.7606 | 756.1349 | 672.7728 | 622.713 | 614.8914 | 668.0593 |
Translational Efficiency | 2.8924 | 3.0537 | 3.1497 | 2.2442 | 3.3104 | 3.0988 | 2.5633 | 3.6276 | 3.1616 | 2.9651 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
692 | 1490 | 1232 | 1171 | 1868 | 2123 | 584 | 1785 | 2560 | 3613 | 1816 | 2956 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 7183.20 | 7675.30 | 13771.30 | 12403.75 | 7413.60 | 7555.03 | 12345.94 | 12207.99 | 7351.32 | 7604.63 | 13312.92 | 12285.54 |
Standard Deviation | 1396.29 | 1790.91 | 2667.47 | 2566.87 | 1537.07 | 1697.86 | 2124.23 | 2553.65 | 1503.80 | 1737.85 | 2592.59 | 2560.68 |
Intensity Change Log 2 | — | 0.095596 | 0.938966 | 0.788078 | — | 0.027263 | 0.735791 | 0.719580 | — | 0.061295 | 0.839350 | 0.753695 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000006 | 0.000054 | 0.000107 | 0.000250 | 0.000029 | 0.000047 | 0.000144 | 0.000115 | 0.000023 | 0.000050 | 0.000119 | 0.000168 |
Bud Neck | 0.000013 | 0.000335 | 0.001244 | 0.000889 | 0.000201 | 0.000291 | 0.001802 | 0.000936 | 0.000150 | 0.000309 | 0.001423 | 0.000917 |
Bud Site | 0.000815 | 0.002736 | 0.002283 | 0.006582 | 0.000894 | 0.001782 | 0.002074 | 0.005780 | 0.000873 | 0.002176 | 0.002216 | 0.006097 |
Cell Periphery | 0.001859 | 0.008477 | 0.011190 | 0.034569 | 0.007909 | 0.004467 | 0.008933 | 0.017996 | 0.006273 | 0.006121 | 0.010464 | 0.024561 |
Cytoplasm | 0.856579* | 0.683443* | 0.457074* | 0.418613* | 0.643471* | 0.711922* | 0.362054* | 0.444913* | 0.701077* | 0.700177* | 0.426517* | 0.434494* |
Cytoplasmic Foci | 0.017136 | 0.011393 | 0.016211 | 0.006933 | 0.009834 | 0.013665 | 0.012091 | 0.007682 | 0.011808 | 0.012728 | 0.014886 | 0.007385 |
Eisosomes | 0.000000 | 0.000001 | 0.000003 | 0.000005 | 0.000001 | 0.000001 | 0.000004 | 0.000002 | 0.000001 | 0.000001 | 0.000003 | 0.000003 |
Endoplasmic Reticulum | 0.000459 | 0.000630 | 0.000523 | 0.000582 | 0.000559 | 0.000448 | 0.000204 | 0.000467 | 0.000532 | 0.000523 | 0.000420 | 0.000512 |
Endosome | 0.000314 | 0.000697 | 0.000705 | 0.000860 | 0.000468 | 0.000683 | 0.000597 | 0.000795 | 0.000426 | 0.000689 | 0.000670 | 0.000821 |
Golgi | 0.039214 | 0.086314 | 0.098794 | 0.051267 | 0.094665 | 0.074825 | 0.066467 | 0.048864 | 0.079676 | 0.079563 | 0.088398 | 0.049816 |
Lipid Particles | 0.001544 | 0.008871 | 0.007753 | 0.014818 | 0.006973 | 0.004200 | 0.008280 | 0.013854 | 0.005506 | 0.006126 | 0.007922 | 0.014236 |
Mitochondria | 0.000266 | 0.000267 | 0.000482 | 0.000779 | 0.000183 | 0.000258 | 0.000483 | 0.000569 | 0.000205 | 0.000262 | 0.000482 | 0.000652 |
Mitotic Spindle | 0.000490 | 0.001146 | 0.001992 | 0.000728 | 0.000822 | 0.001471 | 0.001732 | 0.000913 | 0.000732 | 0.001337 | 0.001908 | 0.000839 |
None | 0.001350 | 0.002799 | 0.002076 | 0.001790 | 0.001993 | 0.003600 | 0.001279 | 0.001426 | 0.001819 | 0.003270 | 0.001820 | 0.001570 |
Nuclear Periphery | 0.000154 | 0.000793 | 0.002719 | 0.000755 | 0.000753 | 0.000469 | 0.002066 | 0.000422 | 0.000591 | 0.000602 | 0.002509 | 0.000554 |
Nuclear Periphery Foci | 0.004682 | 0.014766 | 0.013701 | 0.006346 | 0.012951 | 0.010123 | 0.013253 | 0.005493 | 0.010716 | 0.012038 | 0.013557 | 0.005831 |
Nucleolus | 0.000014 | 0.000076 | 0.000140 | 0.000201 | 0.000076 | 0.000081 | 0.000209 | 0.000204 | 0.000059 | 0.000079 | 0.000162 | 0.000203 |
Nucleus | 0.000897 | 0.007771 | 0.009319 | 0.008485 | 0.005611 | 0.004826 | 0.011860 | 0.006234 | 0.004337 | 0.006040 | 0.010136 | 0.007125 |
Peroxisomes | 0.000009 | 0.000016 | 0.000012 | 0.000047 | 0.000006 | 0.000011 | 0.000009 | 0.000051 | 0.000007 | 0.000013 | 0.000011 | 0.000049 |
Vacuole | 0.010877 | 0.069160 | 0.158944 | 0.283065* | 0.059836 | 0.064025 | 0.318720* | 0.308824* | 0.046602 | 0.066142 | 0.210326* | 0.298620* |
Vacuole Periphery | 0.063321 | 0.100255* | 0.214729* | 0.162439* | 0.152764* | 0.102806* | 0.187742* | 0.134461* | 0.128587* | 0.101754* | 0.206050* | 0.145544* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.39 | -2.64 | -5.17 | -4.20 | -2.05 | -1.87 | -1.90 | -5.14 | -3.82 | 0.24 | -4.11 | -3.00 | -6.99 | -5.63 | -1.19 |
Bud Neck | -7.49 | -11.24 | -10.87 | -6.62 | 2.65 | -2.76 | -6.57 | -4.90 | -4.22 | 3.71 | -5.45 | -11.38 | -7.94 | -6.30 | 4.09 |
Bud Site | -6.16 | -6.57 | -7.18 | -4.71 | -5.12 | -4.98 | -5.71 | -8.44 | -6.60 | -5.24 | -7.83 | -8.44 | -11.05 | -8.01 | -7.43 |
Cell Periphery | -12.04 | -14.01 | -18.53 | -14.21 | -12.24 | 5.93 | -1.09 | -9.47 | -13.47 | -6.56 | 0.22 | -7.07 | -18.70 | -19.21 | -12.93 |
Cytoplasm | 14.42 | 30.85 | 32.46 | 19.25 | 2.04 | -6.25 | 17.55 | 17.46 | 24.62 | -5.20 | 0.39 | 26.65 | 29.10 | 31.16 | -1.34 |
Cytoplasmic Foci | 2.57 | -0.47 | 4.56 | 4.35 | 7.85 | -4.08 | -2.98 | 2.12 | 6.09 | 4.05 | -1.22 | -4.35 | 4.77 | 7.43 | 8.60 |
Eisosomes | -8.55 | -6.31 | -6.09 | -4.71 | -1.88 | -1.24 | -3.03 | -5.92 | -5.49 | 1.21 | -5.08 | -5.91 | -8.22 | -7.02 | -0.14 |
Endoplasmic Reticulum | -2.28 | -0.81 | -1.46 | 0.76 | -0.86 | 1.33 | 4.73 | 1.35 | -0.13 | -5.88 | 0.15 | 2.08 | 0.55 | 0.49 | -2.20 |
Endosome | -7.01 | -8.18 | -1.65 | -0.42 | -0.36 | -3.24 | -2.96 | -4.68 | -1.10 | -1.99 | -5.58 | -6.59 | -2.88 | -0.80 | -0.86 |
Golgi | -10.53 | -13.74 | -2.75 | 9.93 | 14.03 | 5.22 | 8.11 | 14.22 | 9.10 | 5.01 | 0.06 | -2.10 | 11.93 | 13.35 | 15.44 |
Lipid Particles | -8.34 | -11.35 | -14.27 | -4.78 | -6.67 | 3.83 | -1.89 | -6.20 | -10.09 | -3.79 | -1.17 | -4.67 | -10.99 | -10.59 | -7.45 |
Mitochondria | -0.07 | -1.12 | -2.47 | -2.41 | -0.78 | -1.69 | -1.17 | -2.94 | -2.46 | -0.62 | -1.52 | -1.65 | -3.90 | -3.44 | -0.83 |
Mitotic Spindle | -6.44 | -7.85 | -2.17 | 3.58 | 6.45 | -3.40 | -2.38 | -0.70 | 1.41 | 1.93 | -4.92 | -5.72 | -0.97 | 2.14 | 4.26 |
None | -4.76 | -3.65 | -1.23 | 1.91 | 0.55 | -2.16 | 3.33 | 1.96 | 2.70 | -0.55 | -3.18 | -0.12 | 0.85 | 3.27 | 0.80 |
Nuclear Periphery | -8.65 | -12.59 | -13.34 | 0.36 | 9.72 | 3.65 | -7.03 | 4.22 | 0.87 | 8.78 | -0.32 | -12.50 | 0.30 | 0.91 | 13.08 |
Nuclear Periphery Foci | -9.91 | -9.83 | -2.40 | 10.40 | 10.65 | 3.17 | -0.54 | 10.78 | 7.94 | 8.98 | -2.07 | -4.29 | 8.90 | 12.91 | 14.37 |
Nucleolus | -7.75 | -20.89* | -11.01 | -6.71 | -2.80 | -0.55 | -6.06 | -7.50 | -6.79 | 0.60 | -1.79 | -9.32 | -10.69 | -9.44 | -2.47 |
Nucleus | -8.60 | -10.67 | -9.00 | -1.95 | 0.52 | 0.86 | -5.43 | -2.62 | -3.78 | 3.91 | -3.03 | -8.53 | -6.15 | -3.96 | 3.32 |
Peroxisomes | -3.12 | -2.58 | -6.13 | -4.75 | -5.38 | -2.69 | -3.41 | -10.68 | -8.90 | -9.29 | -4.26 | -4.66 | -12.01 | -9.71 | -10.25 |
Vacuole | -16.90 | -29.50 | -37.25 | -26.85 | -13.38 | -1.68 | -24.54 | -37.66 | -36.01 | 1.08 | -7.53 | -31.10 | -50.00 | -44.86 | -12.00 |
Vacuole Periphery | -6.34 | -20.81 | -13.33 | -8.56 | 7.01 | 8.93 | -2.40 | 4.30 | -5.34 | 5.55 | 6.20 | -12.23 | -2.30 | -9.68 | 11.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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