Standard name
Human Ortholog
Description Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.97 0.9 0.68 0.75 0.63 0.69 0.6 0.99 1.0 0.98 0.99 0.99 0.98 0.97 0.94 0.91 0.89 0.9 0.85
Endoplasmic Reticulum 0 0 0 0 0 0.09 0.23 0.18 0.27 0.17 0.25 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.05 0.09 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.12 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 4 7 12 5 16 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 2 0 0 1 3 1 1 1 2 0 0 0 0 0 0 0 0 0 0 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 3 0 0 2 1 9 1 14 4 4 1 1 1 1 0 1 0 0 0 0 0 0
Cytoplasm 570 207 179 177 202 158 187 134 184 139 179 119 200 180 192 307 195 559 197 161 72 162 160
Endoplasmic Reticulum 2 1 0 0 6 15 64 32 79 33 74 1 0 2 5 7 16 3 3 7 1 2 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 14 16 14 6 21 0 0 1 0 1 1 0 0 0 0 0 2
Mitochondria 0 0 0 2 0 0 3 5 9 23 34 0 0 0 0 1 1 0 0 1 0 0 0
Nucleus 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 2 0 1 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 2 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 2 0 1 1 0 0 0 0 1 3 1 4 1 1 2 6 5
Unique Cell Count 573 209 180 178 209 175 275 179 292 200 300 120 200 183 194 311 199 579 209 177 81 181 189
Labelled Cell Count 576 211 181 179 211 178 288 196 315 213 333 121 201 184 199 319 216 579 209 177 81 181 189


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 133.4 170.5 82.9 107.8 94.1 78.1 66.3 63.9 62.9 57.7 57.1 134.0 136.7 127.9 161.1 140.1 153.6 129.0 139.0 135.3
Std Deviation (1e-4) 26.0 31.3 24.4 23.8 21.9 18.7 16.2 15.5 14.8 14.1 12.8 39.7 35.8 31.8 56.0 51.9 46.5 36.8 48.8 50.7
Intensity Change (Log2) 0.38 0.18 -0.09 -0.32 -0.38 -0.4 -0.52 -0.54 0.69 0.72 0.62 0.96 0.76 0.89 0.64 0.75 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.0 0 0 0 0 0 0 0 0
Cytoplasm 0 -1.9 -3.9 -8.3 -7.0 -9.1 -7.9 -9.7 -0.3 1.1 -1.0 -0.5 -0.8 -1.2
Endoplasmic Reticulum 0 0 4.0 7.0 5.9 7.6 5.7 7.2 0 0 0 0 0 3.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3.1 4.1 3.0 0 3.6 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 4.7 4.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 117.455 146.9669 135.3462 129.6613 124.228 133.5966 102.0603 144.0617 127.8778 126.9753 98.4946 115.3738 120.9542 156.4469 141.0917 134.9568 134.1645 127.3908
Actin 0.0012 0.0002 0.0029 0.0004 0.0012 0.0019 0.0051 0.0002 0.0007 0.0008 0.0009 0.0046 0.0028 0.0003 0.0058 0.0011 0.0077 0.0009
Bud 0.0012 0.0007 0.0012 0.0016 0.0013 0.0015 0.0016 0.0005 0.0014 0.0032 0.0009 0.0014 0.0011 0.0003 0.0007 0.0009 0.0011 0.0005
Bud Neck 0.0005 0.0005 0.0006 0.0006 0.0008 0.002 0.0006 0.0003 0.0006 0.0012 0.0006 0.0013 0.0005 0.0004 0.0008 0.0005 0.0007 0.0015
Bud Periphery 0.0014 0.0004 0.0008 0.001 0.0007 0.0019 0.0016 0.0002 0.0007 0.0035 0.0008 0.0013 0.001 0.0001 0.0005 0.0003 0.0011 0.0005
Bud Site 0.0004 0.0003 0.0005 0.0004 0.0002 0.0003 0.001 0.0003 0.0005 0.0004 0.0063 0.0008 0.0006 0.0002 0.0003 0.0005 0.0012 0.0002
Cell Periphery 0.0199 0.0081 0.0121 0.01 0.0038 0.0087 0.0133 0.0067 0.0095 0.0073 0.0016 0.0014 0.0175 0.005 0.0073 0.0025 0.0103 0.0071
Cytoplasm 0.7255 0.8982 0.8229 0.6417 0.7947 0.8292 0.6901 0.9598 0.8194 0.704 0.643 0.7351 0.7315 0.945 0.8628 0.6808 0.7736 0.8427
Cytoplasmic Foci 0.002 0.0024 0.004 0.0034 0.0054 0.006 0.0233 0.0014 0.0054 0.0064 0.0291 0.0063 0.0113 0.0011 0.0057 0.0046 0.0088 0.0087
Eisosomes 0.0003 0 0.0001 0 0 0.0001 0.0006 0 0.0001 0.0005 0.0001 0.0001 0.0006 0 0.0001 0.0001 0.0007 0
Endoplasmic Reticulum 0.0558 0.0079 0.0189 0.0025 0.0105 0.0445 0.0415 0.0026 0.0166 0.0149 0.0035 0.0097 0.0374 0.0048 0.0103 0.0025 0.0042 0.0199
Endosome 0.012 0.0057 0.0096 0.0186 0.03 0.0196 0.0145 0.0022 0.0084 0.0121 0.025 0.0402 0.0119 0.0023 0.0084 0.0172 0.0161 0.0291
Golgi 0.0042 0.0014 0.0036 0.0012 0.0077 0.0073 0.0035 0.0004 0.0013 0.0005 0.0032 0.0264 0.0056 0.0003 0.0016 0.0015 0.0032 0.0031
Lipid Particles 0.0038 0.0007 0.002 0.0012 0.0005 0.0013 0.0093 0.0012 0.0041 0.0026 0.0169 0.0021 0.0069 0.0004 0.002 0.0032 0.0104 0.0032
Mitochondria 0.0076 0.0026 0.0029 0.001 0.0076 0.004 0.0188 0.0002 0.0081 0.0112 0.035 0.0124 0.0085 0.0002 0.0067 0.0194 0.0085 0.0011
None 0.0017 0.0007 0.0009 0.0004 0.0004 0.0009 0.0068 0.0001 0.0054 0.0045 0.0044 0.0021 0.0043 0.0001 0.0012 0.0017 0.0031 0.0004
Nuclear Periphery 0.0192 0.0025 0.0058 0.0077 0.0071 0.0054 0.0243 0.0015 0.0062 0.0058 0.0095 0.0318 0.0193 0.0026 0.0047 0.0053 0.007 0.0081
Nucleolus 0.0043 0.0008 0.0025 0.0012 0.0004 0.0029 0.0105 0.0001 0.0063 0.0079 0.0206 0.0012 0.0063 0.0001 0.0034 0.0049 0.0119 0.0003
Nucleus 0.005 0.0019 0.0028 0.005 0.0045 0.0074 0.0138 0.0011 0.0048 0.0037 0.0089 0.0122 0.0068 0.0013 0.0029 0.0064 0.0075 0.0026
Peroxisomes 0.0004 0.0002 0.0007 0.0001 0.0002 0.0001 0.0042 0 0.0009 0.0009 0.0073 0.0006 0.0099 0 0.0004 0.0053 0.0042 0.0001
Punctate Nuclear 0.0001 0 0.0004 0 0.0001 0.0001 0.0043 0 0.0065 0.0001 0.0137 0.0028 0.0027 0 0.0002 0.0004 0.004 0.0001
Vacuole 0.1072 0.0561 0.0842 0.2315 0.0886 0.0365 0.0822 0.019 0.0778 0.1509 0.1015 0.0709 0.0844 0.0319 0.061 0.1842 0.0843 0.0463
Vacuole Periphery 0.0265 0.0085 0.0205 0.0703 0.0343 0.0184 0.029 0.0022 0.0151 0.0577 0.0671 0.0354 0.0291 0.0035 0.013 0.0567 0.0304 0.0238

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 712.3244 655.5032 622.298 703.8521 707.7606 756.1349 672.7728 622.713 614.8914 668.0593
Translational Efficiency 2.8924 3.0537 3.1497 2.2442 3.3104 3.0988 2.5633 3.6276 3.1616 2.9651

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
692 1490 1232 1171 1868 2123 584 1785 2560 3613 1816 2956

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 7183.20 7675.30 13771.30 12403.75 7413.60 7555.03 12345.94 12207.99 7351.32 7604.63 13312.92 12285.54
Standard Deviation 1396.29 1790.91 2667.47 2566.87 1537.07 1697.86 2124.23 2553.65 1503.80 1737.85 2592.59 2560.68
Intensity Change Log 2 0.095596 0.938966 0.788078 0.027263 0.735791 0.719580 0.061295 0.839350 0.753695

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000006 0.000054 0.000107 0.000250 0.000029 0.000047 0.000144 0.000115 0.000023 0.000050 0.000119 0.000168
Bud Neck 0.000013 0.000335 0.001244 0.000889 0.000201 0.000291 0.001802 0.000936 0.000150 0.000309 0.001423 0.000917
Bud Site 0.000815 0.002736 0.002283 0.006582 0.000894 0.001782 0.002074 0.005780 0.000873 0.002176 0.002216 0.006097
Cell Periphery 0.001859 0.008477 0.011190 0.034569 0.007909 0.004467 0.008933 0.017996 0.006273 0.006121 0.010464 0.024561
Cytoplasm 0.856579* 0.683443* 0.457074* 0.418613* 0.643471* 0.711922* 0.362054* 0.444913* 0.701077* 0.700177* 0.426517* 0.434494*
Cytoplasmic Foci 0.017136 0.011393 0.016211 0.006933 0.009834 0.013665 0.012091 0.007682 0.011808 0.012728 0.014886 0.007385
Eisosomes 0.000000 0.000001 0.000003 0.000005 0.000001 0.000001 0.000004 0.000002 0.000001 0.000001 0.000003 0.000003
Endoplasmic Reticulum 0.000459 0.000630 0.000523 0.000582 0.000559 0.000448 0.000204 0.000467 0.000532 0.000523 0.000420 0.000512
Endosome 0.000314 0.000697 0.000705 0.000860 0.000468 0.000683 0.000597 0.000795 0.000426 0.000689 0.000670 0.000821
Golgi 0.039214 0.086314 0.098794 0.051267 0.094665 0.074825 0.066467 0.048864 0.079676 0.079563 0.088398 0.049816
Lipid Particles 0.001544 0.008871 0.007753 0.014818 0.006973 0.004200 0.008280 0.013854 0.005506 0.006126 0.007922 0.014236
Mitochondria 0.000266 0.000267 0.000482 0.000779 0.000183 0.000258 0.000483 0.000569 0.000205 0.000262 0.000482 0.000652
Mitotic Spindle 0.000490 0.001146 0.001992 0.000728 0.000822 0.001471 0.001732 0.000913 0.000732 0.001337 0.001908 0.000839
None 0.001350 0.002799 0.002076 0.001790 0.001993 0.003600 0.001279 0.001426 0.001819 0.003270 0.001820 0.001570
Nuclear Periphery 0.000154 0.000793 0.002719 0.000755 0.000753 0.000469 0.002066 0.000422 0.000591 0.000602 0.002509 0.000554
Nuclear Periphery Foci 0.004682 0.014766 0.013701 0.006346 0.012951 0.010123 0.013253 0.005493 0.010716 0.012038 0.013557 0.005831
Nucleolus 0.000014 0.000076 0.000140 0.000201 0.000076 0.000081 0.000209 0.000204 0.000059 0.000079 0.000162 0.000203
Nucleus 0.000897 0.007771 0.009319 0.008485 0.005611 0.004826 0.011860 0.006234 0.004337 0.006040 0.010136 0.007125
Peroxisomes 0.000009 0.000016 0.000012 0.000047 0.000006 0.000011 0.000009 0.000051 0.000007 0.000013 0.000011 0.000049
Vacuole 0.010877 0.069160 0.158944 0.283065* 0.059836 0.064025 0.318720* 0.308824* 0.046602 0.066142 0.210326* 0.298620*
Vacuole Periphery 0.063321 0.100255* 0.214729* 0.162439* 0.152764* 0.102806* 0.187742* 0.134461* 0.128587* 0.101754* 0.206050* 0.145544*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.39 -2.64 -5.17 -4.20 -2.05 -1.87 -1.90 -5.14 -3.82 0.24 -4.11 -3.00 -6.99 -5.63 -1.19
Bud Neck -7.49 -11.24 -10.87 -6.62 2.65 -2.76 -6.57 -4.90 -4.22 3.71 -5.45 -11.38 -7.94 -6.30 4.09
Bud Site -6.16 -6.57 -7.18 -4.71 -5.12 -4.98 -5.71 -8.44 -6.60 -5.24 -7.83 -8.44 -11.05 -8.01 -7.43
Cell Periphery -12.04 -14.01 -18.53 -14.21 -12.24 5.93 -1.09 -9.47 -13.47 -6.56 0.22 -7.07 -18.70 -19.21 -12.93
Cytoplasm 14.42 30.85 32.46 19.25 2.04 -6.25 17.55 17.46 24.62 -5.20 0.39 26.65 29.10 31.16 -1.34
Cytoplasmic Foci 2.57 -0.47 4.56 4.35 7.85 -4.08 -2.98 2.12 6.09 4.05 -1.22 -4.35 4.77 7.43 8.60
Eisosomes -8.55 -6.31 -6.09 -4.71 -1.88 -1.24 -3.03 -5.92 -5.49 1.21 -5.08 -5.91 -8.22 -7.02 -0.14
Endoplasmic Reticulum -2.28 -0.81 -1.46 0.76 -0.86 1.33 4.73 1.35 -0.13 -5.88 0.15 2.08 0.55 0.49 -2.20
Endosome -7.01 -8.18 -1.65 -0.42 -0.36 -3.24 -2.96 -4.68 -1.10 -1.99 -5.58 -6.59 -2.88 -0.80 -0.86
Golgi -10.53 -13.74 -2.75 9.93 14.03 5.22 8.11 14.22 9.10 5.01 0.06 -2.10 11.93 13.35 15.44
Lipid Particles -8.34 -11.35 -14.27 -4.78 -6.67 3.83 -1.89 -6.20 -10.09 -3.79 -1.17 -4.67 -10.99 -10.59 -7.45
Mitochondria -0.07 -1.12 -2.47 -2.41 -0.78 -1.69 -1.17 -2.94 -2.46 -0.62 -1.52 -1.65 -3.90 -3.44 -0.83
Mitotic Spindle -6.44 -7.85 -2.17 3.58 6.45 -3.40 -2.38 -0.70 1.41 1.93 -4.92 -5.72 -0.97 2.14 4.26
None -4.76 -3.65 -1.23 1.91 0.55 -2.16 3.33 1.96 2.70 -0.55 -3.18 -0.12 0.85 3.27 0.80
Nuclear Periphery -8.65 -12.59 -13.34 0.36 9.72 3.65 -7.03 4.22 0.87 8.78 -0.32 -12.50 0.30 0.91 13.08
Nuclear Periphery Foci -9.91 -9.83 -2.40 10.40 10.65 3.17 -0.54 10.78 7.94 8.98 -2.07 -4.29 8.90 12.91 14.37
Nucleolus -7.75 -20.89* -11.01 -6.71 -2.80 -0.55 -6.06 -7.50 -6.79 0.60 -1.79 -9.32 -10.69 -9.44 -2.47
Nucleus -8.60 -10.67 -9.00 -1.95 0.52 0.86 -5.43 -2.62 -3.78 3.91 -3.03 -8.53 -6.15 -3.96 3.32
Peroxisomes -3.12 -2.58 -6.13 -4.75 -5.38 -2.69 -3.41 -10.68 -8.90 -9.29 -4.26 -4.66 -12.01 -9.71 -10.25
Vacuole -16.90 -29.50 -37.25 -26.85 -13.38 -1.68 -24.54 -37.66 -36.01 1.08 -7.53 -31.10 -50.00 -44.86 -12.00
Vacuole Periphery -6.34 -20.81 -13.33 -8.56 7.01 8.93 -2.40 4.30 -5.34 5.55 6.20 -12.23 -2.30 -9.68 11.02
Standard name
Human Ortholog
Description Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Egd1

Egd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Egd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available