Standard name
Human Ortholog
Description Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0.06 0.1 0.06 0.06 0.05 0.07 0.05 0 0.07 0.08 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.05 0.05 0.05 0.06 0.05 0 0.07 0.05 0 0 0.06 0.11 0.13 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0.14 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0 0.11 0.15 0.17 0.08 0.06 0.08 0.06 0 0.05
Golgi 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.26 0.34 0.3 0 0.05 0 0.05 0.09 0.11
Mitochondria 0.78 0.88 0.95 0.95 0.91 0.93 0.94 0.93 0.96 0.97 0.93 0.95 0.87 0.71 0.85 0.1 0.11 0.13 0.77 0.75 0.79 0.74 0.69 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.11 0 0.09 0.06 0 0.05 0 0.09 0.08
SpindlePole 0.05 0 0 0 0.06 0 0.05 0.06 0 0 0 0.05 0 0 0 0.07 0.14 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.18 0.05 0 0.06 0 0 0 0 0 0 0 0.19 0.13 0.17 0.19 0.19 0.16 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 2 3 1 0 0 0 0 0 0 0 0 1 1 0 1 4 2 8 4 0 4 7
Bud 2 12 23 44 28 28 34 53 21 19 26 35 1 5 3 2 3 6 0 1 0 0 0 0
Bud Neck 1 0 2 0 1 2 0 0 0 0 1 1 0 0 0 0 2 0 0 0 2 0 0 0
Bud Site 0 2 5 5 1 9 11 18 3 6 8 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 25 8 9 2 4 3 4 4 1 4 5 5 4 3 11 8 8 0 1 0 0 0 1
Cytoplasm 4 18 22 22 21 24 35 50 18 19 25 23 4 4 9 16 19 15 1 2 0 0 0 0
Endoplasmic Reticulum 3 6 6 1 1 3 4 4 3 0 7 0 2 5 3 9 9 0 0 2 0 1 1 0
Endosome 43 18 0 1 9 0 2 1 0 0 0 0 9 16 4 16 22 17 30 36 35 5 8 7
Golgi 24 8 1 0 0 0 0 0 0 0 0 0 4 9 6 39 50 30 13 29 4 4 15 16
Mitochondria 242 492 389 426 407 465 690 737 379 420 345 417 118 90 129 15 17 13 286 441 328 64 129 97
Nucleus 6 1 2 3 4 7 14 16 3 3 3 3 0 1 0 0 4 3 0 1 0 0 0 0
Nuclear Periphery 17 14 2 0 2 13 19 4 8 7 7 2 2 2 2 2 1 2 2 4 4 1 2 2
Nucleolus 6 4 1 1 3 6 4 4 1 2 3 4 1 0 2 3 1 1 1 1 0 0 0 1
Peroxisomes 11 23 7 4 13 1 3 7 4 2 2 4 4 10 1 16 1 9 23 18 21 3 16 12
SpindlePole 15 9 16 19 26 20 35 50 11 11 14 24 0 4 0 11 21 4 0 7 3 0 1 0
Vac/Vac Membrane 51 100 19 6 28 18 28 20 13 9 11 13 25 17 26 29 28 16 5 26 4 3 3 4
Unique Cell Count 311 559 411 449 446 499 736 795 396 435 371 441 135 126 152 149 148 99 372 589 418 87 187 158
Labelled Cell Count 431 734 505 544 547 600 882 968 468 499 456 537 175 168 189 169 187 128 372 589 418 87 187 158


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.1 3.8 4.2 3.9 3.7 3.4 3.4 3.0 3.0 3.1 3.0 5.8 5.8 5.0 7.6 7.9 7.6 5.2 6.6 7.2
Std Deviation (1e-4) 0.8 1.1 0.7 3.4 1.0 1.1 0.8 0.9 0.8 1.0 1.3 3.4 1.0 1.0 0.8 1.9 1.9 2.2 0.9 2.0 1.8
Intensity Change (Log2) – – – 0.16 0.05 -0.04 -0.14 -0.15 -0.33 -0.33 -0.28 -0.31 0.62 0.63 0.42 1.01 1.07 1.02 0.47 0.81 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -2.4 -0.7 -1.8 -2.2 -1.8 0.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0
Cytoplasm -0.3 -0.4 -0.4 -0.4 0.7 -0.5 -0.7 0.8 -0.1 -1.1 -1.0 0.3 2.2 3.0 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 8.0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 10.5 12.2 11.2
Mitochondria 0 0 0 0 0 0 0 0 0 -2.8 -7.3 -3.8 -19.7 -19.5 -17.8
Nucleus 0 0 0 2.0 2.1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 4.8 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1.7 4.3 0
Vacuole -2.9 1.1 -0.8 -0.7 -2.0 -1.0 -2.1 -1.2 -1.3 5.1 3.5 4.8 5.5 5.4 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3663 2.408 2.6216 2.2797 2.2563 2.6328 4.1682 4.4495 4.4082 4.6338 5.136 4.3383 4.5758 4.4453 4.8244 5.1958 4.9478 4.8361
Actin 0.0172 0.016 0.0159 0.0137 0.0033 0.0092 0.0146 0.0134 0.0126 0.0077 0.0339 0.0042 0.028 0.0256 0.0194 0.0018 0.0099 0.0099
Bud 0.0005 0.0012 0.0019 0.0007 0.0014 0.0008 0.001 0.0024 0.0013 0.0017 0.0049 0.0005 0.0006 0.0019 0.0009 0.0033 0.0006 0.0006
Bud Neck 0.0009 0.0007 0.0018 0.0022 0.0003 0.0016 0.0052 0.001 0.0011 0.0007 0.0097 0.0008 0.0008 0.0005 0.0011 0.0007 0.0003 0.0005
Bud Periphery 0.0012 0.0024 0.006 0.0015 0.0046 0.0035 0.0019 0.0045 0.0026 0.0134 0.0099 0.002 0.0014 0.0022 0.0023 0.0094 0.003 0.0017
Bud Site 0.0018 0.0102 0.0066 0.002 0.0017 0.001 0.0049 0.0214 0.0138 0.0006 0.007 0.0006 0.0056 0.0096 0.0074 0.0017 0.0008 0.0008
Cell Periphery 0.0005 0.0026 0.0033 0.0003 0.0008 0.0005 0.0012 0.0009 0.0009 0.0006 0.0012 0.0007 0.0007 0.0004 0.0064 0.0013 0.003 0.0006
Cytoplasm 0.003 0.0027 0.0042 0.0003 0.0095 0.0015 0.0123 0.012 0.0065 0.0018 0.0153 0.0043 0.0029 0.0027 0.0054 0.0226 0.004 0.0034
Cytoplasmic Foci 0.0182 0.0315 0.0177 0.0117 0.0156 0.0172 0.0302 0.025 0.0242 0.0171 0.0279 0.0239 0.0234 0.0473 0.0333 0.0203 0.0232 0.0233
Eisosomes 0.0004 0.0007 0.0009 0.0002 0.0002 0.0003 0.0005 0.0003 0.0011 0.0003 0.0008 0.0002 0.0012 0.0005 0.0016 0.0002 0.0015 0.0004
Endoplasmic Reticulum 0.0037 0.0006 0.0021 0.0006 0.0022 0.001 0.003 0.001 0.0011 0.001 0.0028 0.0014 0.0006 0.0003 0.0011 0.0012 0.0004 0.0024
Endosome 0.0643 0.0576 0.0524 0.0222 0.0569 0.0474 0.0854 0.0755 0.0464 0.0417 0.1085 0.0556 0.0507 0.0489 0.0467 0.083 0.0258 0.0429
Golgi 0.0457 0.0732 0.0479 0.024 0.0597 0.0385 0.0519 0.067 0.0337 0.0461 0.0809 0.0538 0.0813 0.1056 0.0417 0.0336 0.0669 0.0719
Lipid Particles 0.0379 0.0354 0.0174 0.0167 0.0034 0.0166 0.0317 0.0121 0.0057 0.0039 0.004 0.0111 0.0204 0.0128 0.0067 0.0022 0.0068 0.0141
Mitochondria 0.7458 0.7183 0.7832 0.8809 0.7923 0.8161 0.6882 0.7231 0.7984 0.8362 0.6492 0.8056 0.7241 0.6616 0.7891 0.7842 0.7886 0.785
None 0.0025 0.0014 0.001 0.0003 0.0011 0.0011 0.0227 0.0012 0.0136 0.002 0.0029 0.003 0.0063 0.0006 0.0045 0.0015 0.0032 0.0023
Nuclear Periphery 0.0064 0.0002 0.0018 0.0008 0.0025 0.0057 0.0067 0.0003 0.0003 0.0028 0.0084 0.0018 0.0001 0.0001 0.0006 0.0004 0.0001 0.0004
Nucleolus 0.0005 0.0003 0.0006 0.0005 0.0003 0.0007 0.0008 0.0008 0.0007 0.0001 0.0014 0.0003 0.0004 0.0002 0.0005 0.0003 0.0001 0.0001
Nucleus 0.0015 0.0002 0.0008 0.0003 0.0032 0.0008 0.0015 0.0006 0.0007 0.0004 0.005 0.0018 0.0002 0.0001 0.0004 0.0004 0.0001 0.0001
Peroxisomes 0.0327 0.0387 0.0247 0.0162 0.033 0.0282 0.0215 0.0191 0.0261 0.0143 0.0064 0.0199 0.0469 0.0761 0.0259 0.0094 0.0592 0.0362
Punctate Nuclear 0.0009 0.0002 0.0007 0.001 0.0006 0.0007 0.0016 0.0002 0.0002 0.0001 0.0027 0.0013 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001
Vacuole 0.0075 0.0029 0.005 0.0021 0.0037 0.0033 0.007 0.0123 0.0062 0.0028 0.0102 0.0038 0.0021 0.0014 0.0027 0.0127 0.0013 0.0014
Vacuole Periphery 0.0068 0.003 0.0042 0.0019 0.0037 0.0044 0.0061 0.0059 0.0028 0.0047 0.0071 0.0035 0.002 0.0016 0.002 0.0096 0.0011 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.6006 3.9337 8.0272 9.5375 8.3203 9.8955 9.4462 12.863 10.0424 10.7616
Translational Efficiency 0.537 0.8306 0.3474 0.3126 0.3335 0.3446 0.4433 0.3469 0.403 0.3589

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
340 206 1912 254 869 1609 939 75 1209 1815 2851 329

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.49 773.67 1037.18 954.34 667.65 805.66 964.79 967.69 662.26 802.03 1013.34 957.38
Standard Deviation 82.95 104.84 151.34 115.22 103.87 109.04 128.45 123.24 98.81 109.04 148.17 117.23
Intensity Change Log 2 — 0.254634 0.677510 0.557419 — 0.271079 0.531123 0.535453 — 0.263000 0.605107 0.546318

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000839 0.003436 0.002940 0.020455 0.002444 0.002033 0.012315 0.023413 0.001993 0.002192 0.006028 0.021130
Bud Neck 0.024000 0.004431 0.002159 0.012459 0.008154 0.001456 0.007640 0.013419 0.012610 0.001794 0.003964 0.012678
Bud Site 0.023624 0.010368 0.019115 0.119484* 0.006225 0.006105 0.046950 0.134224* 0.011118 0.006589 0.028282 0.122844*
Cell Periphery 0.001163 0.000554 0.000222 0.000425 0.000754 0.000282 0.000312 0.000937 0.000869 0.000313 0.000251 0.000542
Cytoplasm 0.011447 0.003704 0.003639 0.023867 0.003713 0.002267 0.009897 0.015429 0.005888 0.002430 0.005700 0.021944
Cytoplasmic Foci 0.038973 0.054099 0.059611 0.091758 0.031822 0.034203 0.104219 0.100456 0.033833 0.036461 0.074303 0.093740
Eisosomes 0.000116 0.000113 0.000054 0.000138 0.000081 0.000062 0.000128 0.000197 0.000091 0.000068 0.000079 0.000151
Endoplasmic Reticulum 0.000730 0.000344 0.001089 0.002107 0.000943 0.000223 0.002894 0.003014 0.000883 0.000237 0.001684 0.002314
Endosome 0.075347 0.110032 0.080658 0.085390 0.084430 0.077378 0.116986 0.116531 0.081876 0.081084 0.092623 0.092489
Golgi 0.075014 0.095427 0.141727* 0.108038 0.059227 0.101107 0.107068 0.083371 0.063667 0.100462 0.130312* 0.102415
Lipid Particles 0.006467 0.010834 0.000853 0.000599 0.006734 0.002147 0.003425 0.001022 0.006659 0.003133 0.001700 0.000696
Mitochondria 0.651407* 0.537995* 0.470896* 0.230597* 0.720611* 0.713277* 0.255851* 0.222390* 0.701149* 0.693383* 0.400069* 0.228726*
Mitotic Spindle 0.005711 0.023209 0.071585 0.096564 0.004842 0.006376 0.153529* 0.065285 0.005086 0.008287 0.098574 0.089434
None 0.000954 0.000241 0.000393 0.000215 0.000454 0.000425 0.000429 0.000130 0.000595 0.000404 0.000405 0.000195
Nuclear Periphery 0.000194 0.000095 0.000738 0.000179 0.000097 0.000059 0.000687 0.000144 0.000124 0.000063 0.000721 0.000171
Nuclear Periphery Foci 0.001388 0.000152 0.001184 0.000321 0.000491 0.000155 0.001478 0.000411 0.000743 0.000154 0.001281 0.000341
Nucleolus 0.001572 0.001231 0.000411 0.000459 0.000677 0.000186 0.001389 0.000098 0.000929 0.000305 0.000733 0.000377
Nucleus 0.003867 0.006301 0.001069 0.003756 0.001290 0.000208 0.005301 0.000548 0.002015 0.000900 0.002463 0.003025
Peroxisomes 0.032751 0.123332* 0.015556 0.132136* 0.052517 0.031213 0.093478 0.164449* 0.046958 0.041668 0.041220 0.139502*
Vacuole 0.018767 0.008915 0.061251 0.057302 0.005279 0.009080 0.057166 0.046230 0.009072 0.009061 0.059906 0.054778
Vacuole Periphery 0.025670 0.005189 0.064849 0.013750 0.009216 0.011758 0.018857 0.008302 0.013843 0.011013 0.049701 0.012508

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.41 -3.68 -4.68 -3.95 -4.15 0.33 -5.23 -2.43 -2.49 -1.27 -0.21 -4.07 -4.98 -4.97 -3.95
Bud Neck 4.78 5.50 2.44 -3.19 -4.39 3.96 -0.06 -1.33 -3.37 -1.41 6.49 5.00 -0.10 -5.49 -4.27
Bud Site 2.93 1.01 -7.49 -8.74 -8.12 0.10 -13.33 -5.66 -5.67 -3.77 3.07 -9.42 -10.31 -10.78 -8.66
Cell Periphery 1.40 2.70 2.09 0.49 -2.87 2.97 2.83 -0.58 -2.46 -2.37 3.60 4.16 1.97 -2.45 -3.53
Cytoplasm 2.52 2.65 -2.82 -5.82 -6.03 1.17 -4.34 -3.27 -3.88 -1.35 2.79 0.02 -5.45 -7.13 -5.88
Cytoplasmic Foci -2.29 -4.27 -5.93 -4.06 -3.98 -0.75 -15.62 -4.56 -4.44 0.15 -0.90 -13.68 -8.29 -8.12 -2.78
Eisosomes 0.20 5.20 -1.23 -1.35 -7.04 2.76 -5.44 -2.82 -3.33 -1.65 3.69 1.90 -4.24 -6.33 -5.47
Endoplasmic Reticulum 1.70 -1.83 -2.01 -2.58 -1.50 1.12 -2.88 -1.03 -1.47 0.16 1.39 -1.93 -1.79 -3.08 -0.74
Endosome -2.75 -0.67 -0.85 2.03 -0.47 1.11 -4.27 -1.71 -2.15 -0.07 0.15 -2.03 -1.16 -1.35 0.05
Golgi -1.81 -7.76 -2.62 -0.86 3.44 -7.42 -7.35 -1.89 1.43 1.62 -7.23 -13.49 -4.39 -0.04 3.37
Lipid Particles -0.88 2.67 2.78 2.28 1.36 3.37 2.28 3.98 1.26 2.92 2.75 4.49 5.47 3.33 3.90
Mitochondria 3.54 9.08 15.81 9.43 11.38 0.49 30.77 12.99 13.12 0.71 0.57 25.58 23.83 24.55 9.23
Mitotic Spindle -2.76 -17.73 -7.32 -5.29 -1.92 -1.37 -19.10 -3.59 -3.50 4.69 -2.72 -25.83 -8.18 -7.85 0.86
None 1.84 1.49 1.93 0.62 4.68 0.03 0.04 2.48 1.35 2.04 0.71 1.26 2.77 1.10 3.69
Nuclear Periphery 1.14 -4.80 0.11 -1.59 5.93 1.90 -5.76 -0.47 -0.85 3.97 2.32 -8.55 -1.15 -2.92 7.28
Nuclear Periphery Foci 1.09 0.21 0.94 -2.31 5.00 1.64 -3.30 0.26 -1.06 3.33 1.66 -1.57 1.11 -2.37 6.13
Nucleolus 0.44 2.12 2.04 1.33 -0.17 2.16 -1.59 2.70 1.15 2.70 2.58 0.59 2.24 -0.26 1.64
Nucleus -0.65 1.71 -0.10 0.54 -1.21 2.35 -3.59 1.53 -1.87 4.28 1.60 -1.42 -0.73 -1.34 -0.12
Peroxisomes -5.57 3.34 -6.61 -0.38 -8.23 4.10 -5.41 -4.19 -5.06 -2.66 1.19 1.45 -7.12 -7.68 -7.80
Vacuole 2.74 -12.27 -4.98 -6.14 -0.00 -3.88 -13.87 -3.01 -2.73 1.30 -0.04 -23.49 -6.89 -6.90 0.62
Vacuole Periphery 5.57 -8.76 2.85 -3.49 14.57 -1.88 -5.42 0.27 1.06 2.98 1.89 -15.58 0.63 -0.68 13.87
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Ism1

Ism1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ism1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available