Standard name
Human Ortholog
Description Nucleolar protein; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0 – 0 0 0 0 0.07 0 0.09 0.05 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.06 0.14 0.29 0.25 – 0.22 0.25 0.17 0.2 0.07 0.07 0.12 0 0 0 0 0 0 0 0 0.08
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0 – 0 0 0.06 0.05 0 0 0 0.14 0.06 0.11 0 0 0 0 0 0
Nucleus 0.31 0.29 0.27 0 0 0 0.06 – 0.09 0.07 0.09 0.07 0.11 0 0.06 0.05 0.11 0.08 0.19 0.18 0.15 0.18 0.11 0.06
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.67 0.63 0.88 0.79 0.8 0.75 – 0.71 0.74 0.73 0.68 0.79 0.88 0.79 0.81 0.78 0.84 0.75 0.69 0.71 0.6 0.64 0.55
Peroxisomes 0 0 0 0.08 0.13 0.13 0.09 – 0.05 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.35 0.42 0.42 0.46 – 0.44 0.4 0.26 0.4 0.09 0.21 0.21 0 0 0 0 0 0 0 0.08 0.13
Vac/Vac Membrane 0 0.05 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.1 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1
Bud Neck 0 1 0 3 0 3 4 – 0 1 1 2 0 4 1 1 2 3 0 0 1 0 2 3
Bud Site 0 0 0 0 0 0 3 – 3 2 2 5 0 0 0 0 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 8 2 6 6 8 4 – 4 4 6 4 6 9 23 22 24 16 0 1 0 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 5 7 5 0 0 0 0 0 0
Endosome 0 5 5 10 24 76 71 – 36 72 41 39 6 15 28 9 9 12 0 2 2 3 6 17
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 4 2 4 0 0 0 1 0 1
Mitochondria 2 0 0 0 0 2 5 – 7 9 15 10 0 0 0 62 26 55 0 0 1 2 2 9
Nucleus 11 51 35 5 6 5 18 – 14 19 22 14 9 9 15 23 45 41 12 40 26 24 21 13
Nuclear Periphery 1 0 1 0 0 1 0 – 1 1 1 1 2 0 0 0 0 0 0 0 4 2 1 5
Nucleolus 24 118 82 157 138 207 210 – 116 215 173 136 65 191 191 355 321 439 46 156 123 81 125 114
Peroxisomes 0 0 1 14 22 33 25 – 8 6 8 7 1 20 10 2 3 6 0 0 0 0 0 0
SpindlePole 0 1 3 62 73 110 129 – 71 117 62 80 7 46 50 16 12 22 0 3 3 4 15 26
Vac/Vac Membrane 0 8 10 2 4 5 6 – 4 8 8 5 0 1 2 19 15 14 0 11 8 13 11 7
Unique Cell Count 36 176 130 178 175 259 279 163 289 238 199 82 218 243 437 414 521 62 225 175 137 196 208
Labelled Cell Count 39 192 139 260 273 450 475 264 454 339 303 96 295 321 518 466 619 62 225 175 137 196 208


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.1 21.2 15.8 16.4 13.0 10.3 9.3 – 8.2 7.9 7.6 7.5 17.5 17.5 14.8 24.8 24.0 24.0 16.2 15.6 15.9
Std Deviation (1e-4) 4.9 7.5 4.3 3.9 2.8 2.6 2.1 – 1.8 1.7 1.9 1.7 4.8 4.3 4.4 10.0 9.6 8.9 5.7 4.3 4.1
Intensity Change (Log2) – – – 0.05 -0.29 -0.62 -0.77 – -0.96 -1.01 -1.07 -1.07 0.14 0.15 -0.09 0.65 0.6 0.6 0.03 -0.02 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 2.9 1.7 2.0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0.7 2.9 5.8 5.2 – 4.5 5.2 3.7 4.1 0 1.2 2.5 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 2.9 0 0 0 0 4.6 2.9 3.9
Nucleus -6.2 -5.9 -7.7 -5.7 – -4.2 -5.8 -4.5 -5.0 -2.8 -6.2 -5.6 -7.2 -4.5 -6.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.2 3.0 3.6 2.5 – 1.5 2.4 1.9 1.0 2.5 5.4 3.2 4.3 3.3 5.4
Peroxisomes 2.9 3.9 3.9 3.2 – 0 0 0 0 0 3.2 0 0 0 0
SpindlePole 6.9 7.9 8.2 8.8 – 8.1 8.0 5.7 7.7 0 4.9 4.8 0 0 0
Vacuole -2.9 -2.2 -2.8 -2.7 – -2.1 -2.3 -1.8 -2.2 0 0 0 -1.5 -1.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9056 8.9975 8.6543 7.7848 7.2805 8.1284 10.9155 10.4188 10.2539 8.505 9.3851 9.8721 14.663 12.521 12.2088 13.2353 11.8848 11.935
Actin 0.0396 0 0.0385 0.0097 0.0053 0.0381 0.0051 0 0.0046 0.0199 0 0 0.0039 0 0 0 0 0
Bud 0.0007 0 0.0011 0.0002 0.0001 0.0009 0.0004 0 0.0002 0.0002 0.0001 0 0 0 0 0 0.0001 0
Bud Neck 0.0006 0 0.0005 0.0001 0.0002 0.0004 0.0005 0 0.0001 0.0003 0.0001 0.0002 0.0005 0 0 0 0.0001 0.0002
Bud Periphery 0.0009 0 0.0011 0.0002 0.0004 0.0009 0.0005 0 0.0001 0.0002 0.0002 0 0.0001 0 0 0 0.0001 0
Bud Site 0.0028 0 0.0091 0.0006 0.0003 0.0007 0.0068 0 0.0032 0.0012 0.0001 0 0.0006 0 0 0 0.0001 0
Cell Periphery 0.0005 0 0.0001 0 0.0002 0.0001 0.0003 0 0 0 0.0005 0 0.0001 0 0 0 0 0
Cytoplasm 0.0101 0 0.0047 0.0002 0.0001 0.0006 0.0042 0 0.003 0.0009 0.0004 0 0.0001 0 0 0 0 0
Cytoplasmic Foci 0.0135 0 0.0141 0.0061 0.0026 0.0072 0.0118 0 0.0015 0.0055 0.0009 0.0001 0.0002 0 0 0 0 0
Eisosomes 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0029 0 0.0007 0 0.001 0.0003 0.001 0 0.0008 0.001 0.0003 0 0.0003 0 0 0 0 0
Endosome 0.02 0 0.0192 0.0022 0.0131 0.0031 0.0071 0 0.0008 0.0097 0.0009 0 0.0008 0 0 0 0 0
Golgi 0.0072 0 0.0098 0.0056 0.0107 0.0047 0.0029 0 0.0003 0.0064 0 0 0.0003 0 0 0 0 0
Lipid Particles 0.0173 0 0.0024 0.003 0.0324 0.0027 0.0083 0 0.0004 0.0055 0.0037 0.0003 0.0004 0 0 0 0 0
Mitochondria 0.0218 0.0002 0.0057 0.0151 0.0122 0.0028 0.0061 0.0001 0.0002 0.0024 0.0012 0.0002 0.0004 0.0001 0.0001 0.0001 0.0003 0
None 0.0069 0 0.0006 0.0003 0.0003 0.0005 0.0018 0 0.0003 0.0007 0.0014 0 0.0004 0 0 0 0 0
Nuclear Periphery 0.0196 0.0001 0.0017 0.0011 0.0129 0.0018 0.0119 0.0001 0.0012 0.0127 0.0045 0.0003 0.0031 0.0006 0.0001 0 0.0001 0.0001
Nucleolus 0.1614 0.1264 0.0565 0.0424 0.6571 0.2853 0.1102 0.0557 0.0408 0.0638 0.429 0.2565 0.1147 0.0842 0.052 0.0666 0.4848 0.2661
Nucleus 0.6354 0.8729 0.8274 0.8822 0.2438 0.6341 0.7955 0.9437 0.9402 0.8496 0.5383 0.7381 0.8732 0.9145 0.9478 0.9331 0.5133 0.7331
Peroxisomes 0.0069 0 0.0028 0.0148 0.0015 0.0112 0.0058 0 0.0005 0.0024 0.0001 0 0.0001 0 0 0 0 0
Punctate Nuclear 0.0249 0.0003 0.0015 0.0155 0.0032 0.0042 0.0176 0.0004 0.0016 0.0166 0.0069 0.0039 0.0004 0.0005 0 0.0001 0.0008 0.0004
Vacuole 0.0042 0 0.0015 0.0001 0.0007 0.0001 0.0011 0 0.0002 0.0004 0.0061 0.0001 0.0001 0 0 0 0.0001 0
Vacuole Periphery 0.0025 0 0.0008 0.0002 0.0019 0.0001 0.001 0 0 0.0005 0.0053 0.0001 0.0002 0 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.2961 66.6211 59.394 42.4125 64.9962 43.9403 41.434 33.0349 34.1464 47.8592
Translational Efficiency 2.523 2.0287 1.6845 2.0514 1.6245 1.9664 1.8314 1.611 1.445 1.6679

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1808 1159 297 2508 1405 2058 530 133 3213 3217 827 2641

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1124.78 1260.19 1581.62 1862.77 1174.81 1253.69 1668.03 1891.15 1146.66 1256.03 1637.00 1864.20
Standard Deviation 198.98 297.62 260.88 430.89 256.57 283.42 251.52 443.84 227.34 288.63 258.27 431.59
Intensity Change Log 2 — 0.163998 0.491760 0.727807 — 0.093753 0.505718 0.686836 — 0.128539 0.498908 0.707021

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000610 0.000485 0.000751 0.000639 0.000460 0.000915 0.000864 0.000999 0.000544 0.000760 0.000823 0.000658
Bud Neck 0.002762 0.002260 0.006199 0.014037 0.002025 0.005406 0.005818 0.014747 0.002439 0.004272 0.005954 0.014073
Bud Site 0.000607 0.000615 0.000947 0.001883 0.000225 0.001222 0.000698 0.006111 0.000440 0.001004 0.000788 0.002096
Cell Periphery 0.000032 0.000032 0.000016 0.000243 0.000017 0.000134 0.000013 0.000213 0.000026 0.000097 0.000014 0.000242
Cytoplasm 0.000671 0.001136 0.000013 0.000441 0.000121 0.000404 0.000013 0.000678 0.000430 0.000668 0.000013 0.000453
Cytoplasmic Foci 0.000362 0.000224 0.000251 0.001291 0.000115 0.000417 0.000218 0.001658 0.000254 0.000347 0.000230 0.001309
Eisosomes 0.000160 0.000174 0.000198 0.000092 0.000146 0.000200 0.000196 0.000097 0.000154 0.000190 0.000197 0.000092
Endoplasmic Reticulum 0.000697 0.000343 0.000261 0.000512 0.000345 0.000683 0.000283 0.000643 0.000543 0.000561 0.000275 0.000518
Endosome 0.000081 0.000073 0.000007 0.000689 0.000010 0.000474 0.000007 0.004330 0.000050 0.000329 0.000007 0.000872
Golgi 0.000068 0.000328 0.000082 0.001115 0.000036 0.000252 0.000075 0.002445 0.000054 0.000279 0.000077 0.001182
Lipid Particles 0.000098 0.000053 0.000008 0.000087 0.000004 0.000210 0.000007 0.000098 0.000057 0.000153 0.000007 0.000088
Mitochondria 0.000823 0.001147 0.001599 0.001890 0.000769 0.001566 0.001758 0.003622 0.000799 0.001415 0.001701 0.001977
Mitotic Spindle 0.001928 0.001079 0.000166 0.001957 0.000233 0.002168 0.000137 0.011024 0.001187 0.001776 0.000147 0.002414
None 0.000740 0.000939 0.000566 0.000932 0.000970 0.001194 0.000547 0.001629 0.000840 0.001102 0.000554 0.000967
Nuclear Periphery 0.000040 0.000021 0.000018 0.000479 0.000014 0.000066 0.000023 0.000153 0.000028 0.000050 0.000021 0.000462
Nuclear Periphery Foci 0.000447 0.000083 0.000003 0.000219 0.000027 0.000572 0.000005 0.000106 0.000264 0.000396 0.000004 0.000214
Nucleolus 0.155914 0.141142 0.182099 0.032340 0.143637 0.145357 0.183839 0.079781 0.150545 0.143838 0.183214 0.034729
Nucleus 0.828975* 0.842919* 0.781196* 0.938134* 0.843310* 0.832010* 0.779869* 0.863215* 0.835244* 0.835940* 0.780346* 0.934361*
Peroxisomes 0.004287 0.005868 0.025161 0.000922 0.007220 0.004842 0.025216 0.005719 0.005570 0.005211 0.025196 0.001164
Vacuole 0.000272 0.000471 0.000202 0.001126 0.000123 0.001257 0.000186 0.001729 0.000207 0.000973 0.000192 0.001156
Vacuole Periphery 0.000426 0.000609 0.000257 0.000971 0.000196 0.000653 0.000226 0.001004 0.000325 0.000637 0.000237 0.000973

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.37 -1.61 -0.68 -4.14 1.69 -1.69 -14.07 -4.06 -0.67 -1.52 -1.16 -5.06 -2.52 0.27 2.91
Bud Neck 0.74 -9.02 -12.35 -12.14 -8.26 -7.20 -12.16 -4.43 -3.31 -3.21 -6.05 -14.84 -13.22 -10.69 -9.28
Bud Site -0.05 -1.71 -4.38 -3.62 -3.80 -5.36 -11.71 -1.73 -1.46 -1.61 -3.06 -3.07 -5.54 -3.52 -4.69
Cell Periphery 0.14 1.90 -4.89 -4.98 -5.37 -2.71 0.65 -1.61 -0.75 -1.63 -2.54 2.15 -5.30 -3.03 -5.62
Cytoplasm -0.59 1.66 0.20 0.80 -2.75 -1.34 1.14 -1.57 -0.58 -2.37 -0.60 1.87 -0.46 0.19 -2.88
Cytoplasmic Foci 0.78 0.61 -1.86 -2.82 -2.72 -2.62 -1.99 -1.68 -1.31 -1.57 -0.46 0.38 -2.64 -2.57 -3.03
Eisosomes -1.05 -2.83 7.33 7.37 7.98 -4.03 -5.62 2.71 5.19 5.98 -3.69 -5.58 8.56 10.02 13.19
Endoplasmic Reticulum 3.23 3.85 1.32 -2.97 -4.12 -4.14 2.70 -1.37 -0.02 -1.58 -0.19 4.25 -0.10 0.09 -4.65
Endosome 0.16 1.58 -3.33 -3.41 -3.92 -2.95 1.35 -1.08 -0.93 -1.08 -2.64 1.63 -3.30 -1.87 -3.52
Golgi -0.97 -1.39 -2.31 -1.44 -2.26 -2.98 -8.34 -1.07 -0.95 -1.05 -2.14 -3.05 -2.53 -1.91 -2.46
Lipid Particles 0.78 1.70 -0.13 -1.18 -2.94 -2.90 -3.51 -1.55 0.94 -1.51 -1.68 1.67 -1.15 0.76 -3.13
Mitochondria -1.05 -7.74 -8.07 -2.72 -2.42 -1.96 -10.70 -3.99 -2.71 -2.76 -2.18 -13.63 -9.13 -2.36 -2.80
Mitotic Spindle 0.87 2.18 -0.12 -1.23 -4.28 -2.59 0.52 -1.43 -1.17 -1.44 -0.65 2.26 -1.81 -1.13 -4.25
None -1.22 1.26 -1.31 0.16 -3.56 -0.67 1.75 -1.49 -0.92 -2.79 -1.31 2.19 -0.97 0.63 -4.18
Nuclear Periphery 1.02 1.68 -5.65 -5.85 -5.94 -4.57 -3.06 -4.51 -2.93 -4.26 -2.33 0.94 -5.91 -5.61 -6.00
Nuclear Periphery Foci 1.65 1.96 1.01 -2.22 -3.85 -2.76 2.04 -1.51 2.36 -2.11 -0.49 2.03 0.41 1.13 -3.94
Nucleolus 2.43 -2.69 28.85* 21.18* 14.95 -0.32 -4.79 4.46 4.63 6.83 1.65 -5.15 34.40* 31.43* 23.66
Nucleus -2.13 4.28 -20.57 -15.18 -13.44 1.99 6.80 -0.57 -1.17 -3.58 -0.12 7.66 -22.38 -21.20 -20.44
Peroxisomes -2.81 -7.10 13.54 9.40 8.14 4.15 -9.36 0.91 -0.92 8.65 0.98 -12.14 16.10 13.81 14.81
Vacuole -0.03 1.15 -2.22 -2.47 -3.61 -3.82 -4.96 -1.90 -0.64 -1.83 -2.99 0.97 -3.28 -0.70 -3.99
Vacuole Periphery -1.06 1.69 -2.21 -1.35 -3.09 -3.28 -3.14 -1.60 -0.62 -1.54 -2.71 1.55 -2.88 -1.35 -3.34
Standard name
Human Ortholog
Description Nucleolar protein; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Nop4

Nop4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available