Standard name
Human Ortholog
Description MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0.09 0.12 0.16 0.07 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0.08 0.08 0.29 0.24 0.18 0.2 0.24 0.3 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.87 0.89 0.77 0.8 0.77 0.68 0.67 0.63 0.64 0.61 0.61 0.62 0.86 0.8 0.77 0.86 0.85 0.87 0.82 0.8 0.62 0.87 0.73 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.17 0.21 0.2 0.19 0.25 0.35 0.42 0.34 0.46 0.43 0.43 0.1 0.06 0.07 0 0.06 0.05 0.11 0.1 0.24 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.15 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 0 0 3 1 5 7 1 9 2 5 0 0 0 2 0 0 1 1 11 2 5 11
Bud Neck 0 0 0 0 2 1 0 2 0 3 1 2 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 1 0 2 1 6 6 7 8 8 11 12 15 1 1 0 1 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 13 7 22 12 23 15 14 13 5 13 47 64 97 27 25 10 0 0 0 3 6 6
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 3 6 5 0 0 0 2 2 1
Endosome 0 1 0 0 2 0 0 1 0 0 0 0 1 2 6 0 2 4 2 2 6 5 8 17
Golgi 0 0 0 1 0 1 0 0 0 0 0 0 1 1 0 1 1 0 1 3 1 2 3 2
Mitochondria 35 11 9 12 26 96 122 89 99 151 149 138 2 2 2 13 12 7 2 1 8 4 15 15
Nucleus 211 226 278 125 243 226 339 310 313 392 306 365 453 420 470 339 296 226 201 224 252 487 588 597
Nuclear Periphery 1 0 6 3 2 5 5 5 10 6 11 6 3 5 4 7 10 2 1 0 4 0 2 5
Nucleolus 54 43 77 31 59 84 177 204 169 294 214 255 52 29 45 17 21 13 28 28 96 7 29 60
Peroxisomes 1 0 1 0 0 0 0 2 0 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 0 3 2 2 5 3 0 2 1 4 23 15 0 5 2 2 2 6 1 7 10
Vac/Vac Membrane 0 4 5 2 3 3 6 3 6 7 2 7 2 3 5 7 5 9 2 12 12 41 122 164
Unique Cell Count 242 253 363 156 315 333 506 491 492 639 502 593 524 525 613 395 347 261 246 280 407 563 802 905
Labelled Cell Count 307 290 394 182 371 438 686 651 623 887 704 807 568 551 645 417 383 280 246 280 407 563 802 905


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.9 6.9 6.8 6.4 5.3 5.4 5.5 5.6 5.3 5.0 5.4 8.7 8.8 8.2 8.8 8.1 8.1 8.1 8.5 8.8
Std Deviation (1e-4) 0.9 2.1 1.8 2.2 2.1 1.7 1.8 1.6 1.7 1.6 1.6 1.7 2.0 2.3 2.0 1.9 1.8 1.9 1.3 1.8 1.9
Intensity Change (Log2) – – – -0.02 -0.12 -0.38 -0.35 -0.33 -0.31 -0.38 -0.47 -0.37 0.33 0.34 0.25 0.35 0.23 0.23 0.23 0.3 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2.1 2.2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 2.0 0 0.7 -0.4 -0.6 -1.5 -2.6 -1.3 3.1 4.5 5.8 2.0 2.1 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 3.4 9.7 8.8 7.1 7.7 8.8 10.2 8.6 0 0 0 0.7 0.8 0.2
Nucleus 0.9 0.2 -2.6 -3.1 -4.2 -4.1 -4.9 -4.8 -4.8 3.8 1.2 0 3.3 3.0 3.1
Nuclear Periphery 0 0 -0.2 0 0 0.4 0 0.6 0 0 0 0 0.1 1.1 0
Nucleolus -0.3 -0.8 1.3 4.4 6.3 4.2 7.8 6.6 6.9 -4.7 -7.1 -6.3 -7.1 -5.9 -5.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 3.4 2.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0.4 0 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8082 6.1101 5.1334 4.7424 4.0213 5.0692 6.414 6.3265 5.876 5.3803 5.5883 5.9405 5.8688 5.4683 4.9571 4.5914 4.3353 4.8998
Actin 0.0396 0 0.0057 0 0.013 0 0.0086 0 0.0074 0.0212 0.0016 0.0004 0.0273 0 0 0.0002 0 0
Bud 0.0004 0 0.0002 0 0.0004 0 0.0003 0 0.0001 0.0001 0.0002 0 0 0 0 0.0004 0 0
Bud Neck 0.0006 0 0.0001 0.0001 0.0044 0.0005 0.0007 0 0.0008 0.0002 0.0005 0.0003 0.0003 0 0 0.0004 0.0003 0.0003
Bud Periphery 0.0006 0 0.0003 0 0.0006 0 0.0006 0 0.0003 0.0001 0.0003 0 0.0001 0 0 0.0012 0.0001 0.0001
Bud Site 0.0017 0 0.0004 0 0.0049 0 0.0012 0 0.0013 0.0003 0.0012 0 0.0003 0 0 0.0006 0.0001 0
Cell Periphery 0.0002 0 0 0 0.0005 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0 0.0003 0 0
Cytoplasm 0.0046 0 0.0004 0.0004 0.0029 0.0002 0.0014 0 0.0004 0.0014 0.0023 0.0004 0.0004 0 0 0.0003 0 0
Cytoplasmic Foci 0.0118 0 0.0015 0 0.0036 0 0.0085 0 0.0012 0.0028 0.0044 0.0009 0.0006 0 0 0.0002 0 0
Eisosomes 0.0007 0 0 0 0.0002 0 0.0001 0 0 0.0001 0 0 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0013 0 0.0001 0.0001 0.0004 0 0.0015 0 0.001 0.0006 0.0013 0.0005 0.0006 0 0 0.0002 0 0
Endosome 0.0079 0 0.0043 0.0001 0.0035 0.0001 0.0118 0 0.0034 0.0027 0.0089 0.0021 0.0017 0 0 0.0001 0 0
Golgi 0.0036 0 0.0035 0 0.0037 0 0.0035 0 0.0015 0.0024 0.0017 0.0004 0.0009 0 0 0 0 0
Lipid Particles 0.0092 0 0.0008 0 0.0031 0 0.0145 0 0.004 0.0103 0.0068 0.0009 0.0011 0 0 0.0001 0 0
Mitochondria 0.0061 0.0001 0.0021 0.0002 0.0039 0.0001 0.0103 0.0001 0.0014 0.0015 0.0069 0.0002 0.0006 0.0001 0.0001 0.0016 0.0004 0.0002
None 0.0153 0 0.0005 0.0002 0.0033 0.0017 0.0014 0 0.0003 0.001 0.0009 0.0004 0.0028 0 0 0.0002 0 0
Nuclear Periphery 0.0049 0.0007 0.0037 0.0147 0.0094 0.0006 0.0173 0.0007 0.0041 0.0051 0.0127 0.001 0.0009 0.0013 0.001 0.0013 0.0005 0.0002
Nucleolus 0.0436 0.0241 0.0257 0.0184 0.2278 0.0941 0.0395 0.0207 0.0243 0.0107 0.1665 0.0879 0.0331 0.034 0.0309 0.0568 0.1961 0.0822
Nucleus 0.8179 0.9743 0.9472 0.9629 0.6735 0.8986 0.858 0.978 0.9443 0.9262 0.7579 0.9034 0.9274 0.963 0.9677 0.9033 0.8006 0.9166
Peroxisomes 0.0062 0 0.0014 0 0.0017 0 0.003 0 0.0005 0.0049 0.0005 0.0001 0.0004 0 0 0.0001 0 0
Punctate Nuclear 0.0219 0.0005 0.0019 0.0026 0.0379 0.0036 0.0115 0.0004 0.0014 0.0078 0.022 0.0006 0.0006 0.0014 0.0001 0.0319 0.0017 0.0002
Vacuole 0.0013 0 0.0002 0.0001 0.0009 0.0002 0.0034 0 0.001 0.0002 0.0014 0.0002 0.0001 0 0 0.0007 0.0001 0.0001
Vacuole Periphery 0.0007 0 0.0002 0.0002 0.0005 0.0001 0.0024 0 0.0011 0.0003 0.0017 0.0001 0.0001 0 0 0.0003 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.516 31.9357 22.6205 36.618 41.0551 23.5328 32.2901 32.9381 32.1892 30.691
Translational Efficiency 0.9311 0.8321 1.3119 1.0968 1.0358 0.815 0.7179 0.9595 1.0587 1.0691

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1386 556 2081 958 1479 2253 1464 750 2865 2809 3545 1708

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 760.27 983.70 1002.45 1057.43 814.49 934.14 957.11 1120.59 788.26 943.95 983.73 1085.16
Standard Deviation 129.08 165.89 148.17 183.72 124.21 140.98 154.32 189.23 129.46 147.58 152.39 188.78
Intensity Change Log 2 — 0.371707 0.398947 0.475978 — 0.197742 0.232788 0.460290 — 0.284350 0.315398 0.467885

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000422 0.000720 0.000681 0.000881 0.000418 0.000519 0.000711 0.001552 0.000420 0.000559 0.000693 0.001176
Bud Neck 0.002464 0.010183 0.018314 0.015485 0.003728 0.010644 0.008069 0.021652 0.003116 0.010553 0.014083 0.018193
Bud Site 0.000798 0.000773 0.000914 0.001077 0.000365 0.000867 0.000673 0.004484 0.000574 0.000849 0.000814 0.002573
Cell Periphery 0.000063 0.000036 0.000031 0.000037 0.000048 0.000059 0.000072 0.000108 0.000055 0.000055 0.000048 0.000068
Cytoplasm 0.001434 0.000361 0.000165 0.000458 0.000586 0.000476 0.001344 0.002427 0.000996 0.000454 0.000652 0.001323
Cytoplasmic Foci 0.000188 0.000060 0.000023 0.000135 0.000225 0.000357 0.000095 0.001729 0.000207 0.000299 0.000053 0.000835
Eisosomes 0.000024 0.000043 0.000040 0.000028 0.000024 0.000033 0.000035 0.000026 0.000024 0.000035 0.000038 0.000027
Endoplasmic Reticulum 0.001902 0.002133 0.001658 0.001854 0.002096 0.002401 0.002514 0.002399 0.002003 0.002348 0.002011 0.002093
Endosome 0.000178 0.000267 0.000082 0.000443 0.000173 0.000626 0.000561 0.002183 0.000175 0.000555 0.000280 0.001207
Golgi 0.000152 0.000256 0.000140 0.000197 0.000182 0.000443 0.000120 0.001733 0.000167 0.000406 0.000131 0.000871
Lipid Particles 0.000223 0.000041 0.000022 0.000035 0.000253 0.000340 0.000078 0.000685 0.000238 0.000281 0.000045 0.000320
Mitochondria 0.000987 0.001328 0.001227 0.002328 0.000857 0.001454 0.000975 0.005534 0.000920 0.001429 0.001123 0.003736
Mitotic Spindle 0.001841 0.001600 0.001669 0.006117 0.002039 0.004280 0.001139 0.013286 0.001943 0.003749 0.001450 0.009265
None 0.006857 0.001715 0.000994 0.001968 0.002662 0.001457 0.006703 0.003906 0.004692 0.001508 0.003352 0.002819
Nuclear Periphery 0.000207 0.000644 0.000370 0.000965 0.000259 0.000719 0.000468 0.003569 0.000234 0.000704 0.000411 0.002108
Nuclear Periphery Foci 0.001212 0.000337 0.000197 0.000297 0.000577 0.001011 0.000224 0.001832 0.000884 0.000878 0.000208 0.000971
Nucleolus 0.139152 0.115636 0.100646 0.095078 0.116097 0.109925 0.063086 0.080723 0.127250 0.111056 0.085135 0.088774
Nucleus 0.840335* 0.861294* 0.871468* 0.869601* 0.868516* 0.861939* 0.911818* 0.845434* 0.854883* 0.861811* 0.888132* 0.858989*
Peroxisomes 0.000588 0.001017 0.000644 0.000807 0.000565 0.000604 0.000549 0.000966 0.000576 0.000685 0.000605 0.000877
Vacuole 0.000386 0.000783 0.000296 0.001211 0.000151 0.000815 0.000465 0.003802 0.000265 0.000809 0.000366 0.002349
Vacuole Periphery 0.000588 0.000777 0.000420 0.000999 0.000179 0.001029 0.000300 0.001971 0.000377 0.000979 0.000370 0.001426

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.43 -10.19 -12.76 -4.04 -5.65 -2.75 -9.09 -4.77 -4.38 -3.34 -5.04 -13.43 -7.31 -6.08 -4.36
Bud Neck -9.44 -24.12 -14.66 -5.44 2.50 -9.62 -11.17 -12.04 -7.39 -8.54 -12.64 -25.79 -18.29 -8.85 -4.33
Bud Site 0.06 -0.64 -0.89 -2.08 -0.69 -3.13 -5.23 -3.65 -3.15 -3.24 -1.10 -1.80 -3.77 -3.45 -3.29
Cell Periphery 2.01 2.90 2.27 -0.33 -1.57 -0.52 -1.38 -2.46 -1.82 -1.51 0.13 0.88 -0.87 -0.80 -2.00
Cytoplasm 2.28 2.82 2.11 -0.61 -2.58 0.46 -2.11 -2.82 -3.03 -1.48 2.02 1.51 -0.50 -2.72 -2.25
Cytoplasmic Foci 1.79 2.23 1.11 -0.74 -1.35 -0.58 0.83 -2.19 -1.92 -2.63 -0.39 1.92 -1.73 -1.35 -2.85
Eisosomes -6.66 -13.94 -4.09 4.41 8.13 -6.69 -8.99 -3.46 1.42 3.47 -9.77 -16.41 -5.30 3.77 8.25
Endoplasmic Reticulum -1.14 0.98 -0.30 0.90 -1.19 -1.98 -3.67 -3.08 -1.78 -0.88 -2.98 -1.50 -2.47 0.21 -1.59
Endosome -0.59 2.16 -1.13 -0.57 -1.67 -2.81 -0.93 -2.75 -1.86 -1.77 -2.91 -0.57 -2.97 -1.40 -2.29
Golgi -0.88 0.20 -1.36 0.38 -1.40 -1.09 1.07 -1.54 -1.17 -1.63 -1.28 0.96 -1.62 -0.83 -1.73
Lipid Particles 1.67 1.81 1.66 -0.05 -1.53 -0.35 0.96 -0.95 -0.69 -1.87 -0.21 1.81 -0.12 0.10 -1.96
Mitochondria -1.17 -2.41 -7.86 -3.70 -7.04 -2.35 -1.29 -4.67 -3.97 -4.51 -2.54 -2.82 -6.49 -4.98 -5.88
Mitotic Spindle 0.37 -0.24 -3.45 -3.59 -3.38 -1.42 0.56 -4.63 -3.97 -4.96 -1.53 0.16 -5.64 -4.70 -5.79
None 4.70 5.93 4.50 -0.72 -2.93 2.41 -3.17 -0.86 -2.75 2.30 5.61 2.26 3.08 -2.90 0.84
Nuclear Periphery -0.94 -1.18 -3.01 -0.64 -2.08 -1.99 -0.90 -4.65 -3.75 -4.13 -2.29 -1.40 -5.46 -3.42 -4.62
Nuclear Periphery Foci 2.55 2.89 2.54 -0.09 -1.14 -1.19 0.98 -2.77 -1.60 -3.89 0.19 2.98 -0.08 -0.29 -4.02
Nucleolus 3.29 6.51 6.18 2.15 0.96 1.23 10.02 4.71 3.96 -2.99 4.18 10.41 7.42 3.87 -0.88
Nucleus -2.67 -4.11 -2.18 0.47 0.95 1.28 -6.77 3.66 2.95 7.73 -1.56 -6.55 1.29 2.41 5.92
Peroxisomes -3.87 -2.26 -3.98 1.00 -2.54 -0.30 -0.27 -3.76 -4.24 -4.75 -1.48 -1.45 -5.18 -3.65 -5.10
Vacuole -0.79 0.36 -1.52 -0.58 -1.78 -2.68 -2.49 -3.70 -3.01 -3.35 -2.31 -1.11 -4.01 -2.85 -3.80
Vacuole Periphery -0.35 0.44 -0.54 -0.18 -1.22 -2.34 -0.83 -3.92 -1.03 -3.44 -1.70 0.11 -2.51 -0.48 -3.01
Standard name
Human Ortholog
Description MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Dig1

Dig1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dig1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available