Standard name
Human Ortholog
Description Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.15 0.09 0.24 0.33 0.4 0.29 0.26 – 0.46 0.61 0.64 0.27 0.6 0.75 0.2 0.13 0.24 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0.07 0 0.12 0 0 0 0 0 – 0 0 0 0.09 0 0 0.05 0.05 0.08 0 0.05 0.08 0.11 0.06 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.08 0.08 0 0 0 0 0.05 – 0.06 0.08 0.07 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nucleus 0.12 0.1 0.08 0.11 0 0.1 0.19 0.17 – 0.08 0.07 0 0.11 0.06 0 0.27 0.18 0.19 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.78 0.79 0.79 0.8 0.79 0.74 0.74 0.76 – 0.65 0.57 0.55 0.68 0.42 0.31 0.56 0.68 0.6 0.87 0.88 0.86 0.8 0.87 0.84
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 – 0 0 0 1 0 1 1 0 1 0 0 1 0 1 0
Bud 0 1 0 0 0 0 1 1 – 2 2 2 0 0 1 0 0 0 0 3 1 4 7 7
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 40 30 78 137 159 149 134 – 228 250 337 77 205 322 33 32 31 0 5 1 4 4 9
Endoplasmic Reticulum 1 2 3 2 8 2 2 5 – 3 0 1 6 7 1 9 14 5 0 0 2 5 4 1
Endosome 8 4 43 13 10 8 1 2 – 0 0 0 26 4 7 9 13 10 4 15 30 44 45 50
Golgi 1 0 1 0 0 0 0 0 – 0 0 0 0 0 0 2 8 3 2 1 1 1 3 1
Mitochondria 14 23 27 6 1 6 2 24 – 31 34 34 2 1 0 8 12 3 3 1 2 4 7 5
Nucleus 13 28 27 37 12 42 95 89 – 42 27 16 31 20 8 45 46 25 0 3 3 1 3 7
Nuclear Periphery 0 0 3 1 1 0 1 4 – 2 0 2 0 0 0 0 2 0 2 0 3 2 8 16
Nucleolus 2 2 8 1 1 0 1 1 – 0 0 1 0 0 0 3 3 1 0 1 1 0 4 2
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 0 0 0 0 0 – 0 1 0 0 0 0 2 0 0 0 1 0 1 2 2
Vac/Vac Membrane 83 217 276 256 331 298 377 388 – 324 235 286 195 144 133 93 172 78 110 262 328 312 622 576
Unique Cell Count 107 275 349 322 419 400 511 513 499 411 523 285 342 432 167 253 131 127 300 381 390 719 688
Labelled Cell Count 131 317 421 394 502 515 629 648 632 549 679 338 382 473 205 302 157 127 300 381 390 719 688


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.8 7.0 8.4 9.6 8.9 8.5 7.7 – 6.7 6.2 6.2 6.7 5.9 6.1 7.6 9.4 10.6 8.7 8.0 8.5
Std Deviation (1e-4) 1.4 1.7 1.5 2.1 2.0 2.5 2.5 2.2 – 2.0 1.9 1.8 1.1 1.0 1.0 2.6 3.6 3.8 1.8 1.6 1.8
Intensity Change (Log2) – – – 0.25 0.44 0.33 0.27 0.13 – -0.08 -0.18 -0.17 -0.07 -0.25 -0.22 0.11 0.41 0.59 0.3 0.18 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 5.5 8.1 9.8 7.3 6.4 – 11.6 14.9 16.4 6.2 14.2 18.4 3.6 1.6 4.4
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 3.4 0
Endosome -3.9 -5.4 -5.6 -7.9 -7.7 – -8.0 -7.3 -8.2 -1.3 -5.8 -6.1 -2.4 -3.0 -1.5
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -3.5 -5.5 -4.1 -5.9 -1.9 – -0.9 0.3 -0.7 -4.2 -5.0 -5.9 -1.2 -1.5 -2.2
Nucleus 1.7 -3.1 1.3 4.5 4.1 – 0.4 -0.6 -3.1 1.4 -1.0 -4.0 5.9 3.9 3.6
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0.1 0 -1.5 -1.8 -1.2 – -4.5 -6.4 -7.4 -3.1 -10.0 -13.4 -5.5 -3.1 -4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7839 6.7478 5.9328 5.7021 5.6956 6.0782 4.6626 7.6464 6.6184 6.3214 6.2439 6.066 4.4229 8.099 6.7921 6.7102 6.8432 6.5301
Actin 0 0 0.0001 0 0 0.0001 0.0358 0 0.0001 0.0007 0.0001 0.004 0.0041 0 0.001 0 0 0.0033
Bud 0.0002 0.0004 0.0005 0.0002 0.0003 0.0003 0.0006 0.0002 0.0004 0.0003 0.0002 0.0003 0.0003 0.0002 0.0003 0.0003 0.0002 0.0005
Bud Neck 0.0007 0.0014 0.0046 0.001 0.0012 0.0012 0.0014 0.001 0.0011 0.0012 0.0014 0.0013 0.0012 0.001 0.0009 0.0039 0.0014 0.0013
Bud Periphery 0.0007 0.0009 0.0015 0.0005 0.0009 0.0013 0.0013 0.0011 0.0016 0.0008 0.0005 0.0012 0.0007 0.0008 0.0012 0.0007 0.0007 0.0022
Bud Site 0.0004 0.0008 0.0013 0.0007 0.0006 0.0005 0.0037 0.0006 0.0012 0.001 0.0005 0.0006 0.001 0.0006 0.0011 0.0014 0.0007 0.0008
Cell Periphery 0.0037 0.0053 0.0037 0.0024 0.0035 0.0041 0.0039 0.004 0.0057 0.0047 0.0021 0.0062 0.0026 0.0087 0.0042 0.0023 0.0035 0.0061
Cytoplasm 0.0013 0.005 0.0023 0.0024 0.0007 0.001 0.0092 0.0008 0.0011 0.0027 0.0016 0.0009 0.0028 0.0013 0.002 0.0007 0.0008 0.0008
Cytoplasmic Foci 0.0029 0.0067 0.0105 0.0052 0.0015 0.0013 0.0086 0.0004 0.004 0.0044 0.0092 0.0046 0.0179 0.0023 0.0108 0.0026 0.0018 0.0023
Eisosomes 0 0 0 0 0 0 0.0005 0 0 0 0 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0016 0.0073 0.0053 0.0006 0.0008 0.0088 0.0062 0.0029 0.0033 0.0024 0.0018 0.0017 0.0041 0.004 0.0035 0.0006 0.0011 0.003
Endosome 0.1443 0.0535 0.1059 0.0928 0.1128 0.0831 0.1657 0.0529 0.1706 0.0817 0.1578 0.089 0.2421 0.0836 0.1752 0.1567 0.1142 0.1011
Golgi 0.0038 0.001 0.0037 0.0013 0.0036 0.0025 0.0164 0.0022 0.019 0.0069 0.0132 0.0064 0.0162 0.0031 0.0195 0.0055 0.0024 0.0093
Lipid Particles 0.0101 0.0028 0.0042 0.0018 0.0084 0.0065 0.0485 0.0033 0.0224 0.0165 0.0637 0.0275 0.0284 0.0174 0.0291 0.012 0.0091 0.018
Mitochondria 0.0066 0.0008 0.0166 0.0004 0.0006 0.0047 0.0474 0.0029 0.0506 0.0102 0.0049 0.0271 0.025 0.0021 0.0389 0.0043 0.0014 0.0199
None 0.0001 0.0003 0.0003 0 0 0.0001 0.0063 0 0.0002 0.0001 0.0001 0.0002 0.004 0.0001 0.0009 0.0001 0.0001 0.0001
Nuclear Periphery 0.0022 0.0041 0.0064 0.0023 0.0021 0.0088 0.021 0.0053 0.0036 0.0044 0.0022 0.0044 0.014 0.0064 0.0079 0.001 0.0016 0.0067
Nucleolus 0.0063 0.0045 0.0061 0.0025 0.0048 0.0048 0.0059 0.0015 0.0057 0.0051 0.0019 0.0046 0.0032 0.0025 0.0033 0.0064 0.0033 0.0052
Nucleus 0.0034 0.0051 0.0082 0.002 0.0127 0.004 0.0078 0.0024 0.0035 0.0032 0.0017 0.0033 0.0023 0.002 0.0037 0.0019 0.0018 0.0045
Peroxisomes 0.0006 0.0002 0.0019 0.0002 0.0004 0.0071 0.0032 0.0001 0.0008 0.0006 0.0021 0.0008 0.0092 0.0004 0.0019 0.0009 0.0007 0.0016
Punctate Nuclear 0.0001 0.0001 0.0001 0 0 0.0001 0.0158 0 0.0001 0.0001 0.0003 0.0002 0.0034 0.0001 0.0011 0.0001 0 0.0001
Vacuole 0.5988 0.7187 0.6377 0.7122 0.7069 0.6567 0.4011 0.6268 0.5054 0.6512 0.5614 0.5571 0.4283 0.629 0.4702 0.6103 0.7035 0.5855
Vacuole Periphery 0.2121 0.1813 0.1791 0.1714 0.1381 0.2029 0.1898 0.2915 0.1997 0.2018 0.1731 0.2583 0.1892 0.2344 0.2234 0.1884 0.1517 0.2278

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.4204 44.1332 37.661 43.4669 54.9415 32.8947 46.6682 42.1645 46.3772 56.9127
Translational Efficiency 1.3113 1.6406 1.3547 1.0791 1.0907 1.8647 1.3485 1.0916 0.9613 0.8574

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (93%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ktr6

Ktr6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ktr6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available