Standard name
Human Ortholog
Description Mitochondrial inner membrane magnesium transporter; involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.11 0 0 0 0 0 0 0 0
Bud 0.05 0 0.05 0 0 0.05 0.06 0.06 0.06 0.06 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0.1 0.16 0.05 0.1 0.09 0.07 0.07 0.06 0.1 0 0 0.05 0.57 0.64 0.65 0.23 0.22 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0.13 0 0 0.07 0 0.07 0 0 0 0 0 0
Endosome 0 0 0.14 0 0.13 0 0 0 0 0 0 0 0.09 0.14 0.14 0.08 0.18 0.17 0.13 0.12 0.12 0.07 0.07 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.88 0.77 0.69 0.81 0.77 0.9 0.91 0.87 0.83 0.96 0.94 0.97 0 0 0 0.18 0.14 0.12 0.67 0.63 0.61 0.7 0.67 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.32 0.06 0.09 0 0 0 0 0 0 0 0 0 0.08 0 0.16 0.05 0 0 0.12 0 0 0
SpindlePole 0.05 0 0.13 0.05 0.07 0 0.07 0.11 0.06 0 0 0 0.08 0.06 0 0.18 0.17 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0 0.13 0.05 0 0.07 0 0 0 0 0 0 0 0.07 0.16 0.09 0.14 0.18 0.16 0 0.08 0 0.06 0.08 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 2 1 0 0 0 0 0 0 0 13 2 5 11 2 3 7 6 5 0 13 12
Bud 16 1 8 6 7 13 25 26 16 22 11 10 0 2 1 5 9 1 0 1 2 0 3 2
Bud Neck 1 0 1 0 0 0 0 1 0 0 0 0 3 1 1 0 2 1 5 1 1 1 7 4
Bud Site 2 0 0 3 1 1 10 13 2 11 4 7 0 0 0 3 2 1 – – – – – –
Cell Periphery 2 1 8 4 2 1 0 2 5 2 1 2 6 6 3 5 9 6 0 0 0 0 0 1
Cytoplasm 32 21 8 16 21 18 27 27 26 10 10 13 99 154 207 23 27 17 2 4 0 2 7 5
Endoplasmic Reticulum 13 6 3 3 1 5 0 1 2 2 0 5 22 4 12 7 3 5 2 2 1 1 2 2
Endosome 0 1 21 6 29 0 2 10 7 0 1 0 16 34 44 8 22 13 43 21 24 10 24 30
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 4 0 1 1 3 6 2 5 2 7 10
Mitochondria 286 102 104 124 175 222 373 390 213 336 239 264 4 5 12 18 17 9 230 112 120 100 255 250
Nucleus 0 1 1 1 2 3 9 10 8 9 7 2 3 0 1 5 1 4 0 0 0 2 6 0
Nuclear Periphery 2 1 0 0 1 2 4 0 2 4 4 7 1 1 0 0 1 0 5 1 1 2 2 2
Nucleolus 0 0 0 1 0 1 4 2 1 1 6 3 0 0 0 0 0 0 0 0 1 0 0 1
Peroxisomes 0 0 48 10 20 5 12 15 5 2 1 1 0 5 24 2 19 4 13 5 24 1 5 13
SpindlePole 15 2 19 7 15 5 27 48 15 13 8 11 13 15 14 18 21 14 5 2 3 1 7 6
Vac/Vac Membrane 13 17 7 3 15 8 5 11 7 9 8 6 12 38 28 14 22 12 10 15 1 8 29 14
Unique Cell Count 325 132 151 154 227 248 410 447 258 349 255 273 173 242 317 99 121 76 345 180 197 144 380 365
Labelled Cell Count 383 155 230 186 290 284 498 556 309 421 300 331 192 271 352 120 158 93 345 180 197 144 380 365


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.6 4.6 4.3 4.6 3.7 3.5 3.5 3.6 3.2 3.0 3.0 5.7 5.9 5.4 5.7 5.9 5.9 5.8 6.2 6.4
Std Deviation (1e-4) 0.5 0.7 1.2 1.5 2.2 1.8 1.5 1.2 1.3 0.8 1.1 0.7 0.8 1.2 0.9 1.1 1.6 1.4 1.2 1.6 1.4
Intensity Change (Log2) – – – -0.11 -0.01 -0.31 -0.39 -0.41 -0.37 -0.54 -0.65 -0.65 0.31 0.34 0.22 0.3 0.35 0.35 0.33 0.43 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.6 3.6 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2926 -0.589 -1.0598 -1.1734 -1.0329 -0.7613 1.2593 0.4278 0.4997 0.2054 0.686 0.5109 -0.1932 0.2735 0.0476 0.2963 -0.0575 0.0182
Actin 0.0056 0.0055 0.0055 0.0017 0.0099 0.0064 0.0407 0.0191 0.0324 0.1088 0.027 0.0125 0.0289 0.0138 0.0128 0.0215 0.0029 0.0192
Bud 0.0013 0.0056 0.008 0.0013 0.0063 0.0007 0.0019 0.003 0.0026 0.0023 0.0014 0.0012 0.0014 0.0019 0.0038 0.0008 0.0036 0.0008
Bud Neck 0.0018 0.0006 0.0031 0.001 0.0014 0.0023 0.0036 0.002 0.0092 0.0017 0.0068 0.0065 0.0018 0.0024 0.0027 0.0044 0.0011 0.0193
Bud Periphery 0.0036 0.0104 0.008 0.0051 0.0099 0.0018 0.0039 0.0069 0.0079 0.0062 0.0037 0.003 0.0037 0.0034 0.0057 0.002 0.0053 0.0013
Bud Site 0.0039 0.0073 0.013 0.001 0.0031 0.0009 0.0081 0.0321 0.0324 0.0038 0.0072 0.001 0.0046 0.0534 0.0202 0.0017 0.014 0.0035
Cell Periphery 0.0008 0.0004 0.0008 0.0004 0.0009 0.0004 0.001 0.0026 0.0013 0.0013 0.0012 0.0009 0.0031 0.0016 0.0037 0.0029 0.0009 0.0008
Cytoplasm 0.0174 0.0141 0.0161 0.0292 0.0375 0.0136 0.0497 0.0854 0.0287 0.0786 0.017 0.0865 0.0307 0.0661 0.0406 0.0322 0.0849 0.0524
Cytoplasmic Foci 0.0966 0.0972 0.0927 0.1241 0.1093 0.1192 0.0834 0.1544 0.1248 0.0912 0.1232 0.1492 0.1528 0.1354 0.0946 0.1074 0.1149 0.1484
Eisosomes 0.0011 0.0003 0.0013 0.0002 0.0007 0.0009 0.0006 0.0011 0.0014 0.001 0.0006 0.0004 0.0052 0.0006 0.001 0.0136 0.0003 0.0005
Endoplasmic Reticulum 0.0025 0.0011 0.0026 0.0009 0.0028 0.0012 0.0146 0.0089 0.0021 0.0016 0.0016 0.0061 0.0048 0.0079 0.0035 0.0032 0.0067 0.0028
Endosome 0.1532 0.152 0.0805 0.0772 0.1915 0.1136 0.2247 0.1777 0.0854 0.227 0.0951 0.1856 0.2248 0.1529 0.137 0.1365 0.2104 0.2043
Golgi 0.042 0.0805 0.0322 0.026 0.0541 0.0447 0.0852 0.083 0.0561 0.0426 0.0814 0.0834 0.0759 0.0999 0.0804 0.0728 0.0904 0.0985
Lipid Particles 0.0954 0.0156 0.0415 0.0203 0.1032 0.0702 0.06 0.0342 0.0243 0.0234 0.1194 0.0237 0.071 0.0151 0.0196 0.0203 0.0158 0.0288
Mitochondria 0.3227 0.4561 0.3905 0.4471 0.232 0.3595 0.177 0.2288 0.3991 0.256 0.3053 0.3605 0.1984 0.345 0.4144 0.4596 0.2983 0.2491
None 0.1538 0.0066 0.1776 0.1045 0.1174 0.1176 0.1088 0.08 0.126 0.0118 0.0863 0.0367 0.1061 0.0426 0.0941 0.0571 0.1176 0.0758
Nuclear Periphery 0.0032 0.0003 0.0033 0.0005 0.0061 0.0017 0.0443 0.0024 0.0006 0.0005 0.0029 0.0047 0.0016 0.0026 0.0055 0.0011 0.0012 0.001
Nucleolus 0.0056 0.0005 0.0052 0.0015 0.0057 0.0022 0.0012 0.0009 0.0012 0.0003 0.0016 0.0006 0.0008 0.0011 0.0007 0.001 0.0007 0.0004
Nucleus 0.0052 0.0007 0.007 0.002 0.0072 0.002 0.0171 0.0013 0.0012 0.0004 0.0014 0.001 0.0009 0.0012 0.0048 0.0016 0.001 0.0009
Peroxisomes 0.0623 0.1338 0.0883 0.1384 0.074 0.1227 0.0395 0.055 0.049 0.1254 0.1031 0.0198 0.0579 0.0385 0.0452 0.0446 0.0152 0.0774
Punctate Nuclear 0.006 0.0003 0.0082 0.0032 0.0101 0.007 0.0101 0.0013 0.004 0.0005 0.0082 0.0031 0.0013 0.0012 0.0013 0.0015 0.0025 0.0038
Vacuole 0.0117 0.0073 0.0099 0.0103 0.0113 0.008 0.0171 0.0146 0.0077 0.0102 0.0036 0.0087 0.0181 0.0095 0.0055 0.0083 0.0079 0.0072
Vacuole Periphery 0.0043 0.0039 0.0047 0.0041 0.0055 0.0035 0.0075 0.0054 0.0025 0.0054 0.0022 0.0049 0.0062 0.0039 0.0029 0.0059 0.0045 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.9978 6.3535 3.5359 3.9509 2.8797 6.2154 6.0225 5.6907 5.0923 4.7232
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1849 995 2072 1901 1625 1568 72 177 3474 2563 2144 2078

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 690.20 756.35 783.78 770.22 676.01 703.47 864.32 805.41 683.56 724.00 786.48 773.22
Standard Deviation 77.33 96.70 94.10 88.51 78.97 97.12 117.23 89.02 78.42 100.32 96.07 89.09
Intensity Change Log 2 — 0.132040 0.183434 0.158256 — 0.057444 0.354521 0.252679 — 0.095611 0.270625 0.205750

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001282 0.001624 0.002330 0.002678 0.000691 0.000608 0.003242 0.002950 0.001005 0.001002 0.002361 0.002701
Bud Neck 0.019139 0.020005 0.040851 0.044640 0.018295 0.018725 0.022079 0.018661 0.018744 0.019222 0.040221 0.042427
Bud Site 0.011935 0.026980 0.033506 0.030274 0.014354 0.014231 0.011651 0.026671 0.013067 0.019180 0.032772 0.029967
Cell Periphery 0.000370 0.000384 0.000179 0.000178 0.000200 0.000209 0.000397 0.000289 0.000290 0.000277 0.000187 0.000187
Cytoplasm 0.033584 0.022812 0.288263* 0.244881* 0.037807 0.022859 0.151482* 0.226708* 0.035560 0.022841 0.283669* 0.243333*
Cytoplasmic Foci 0.170925 0.150521 0.139815 0.148517 0.230802* 0.147721 0.233868* 0.235234* 0.198933* 0.148808 0.142973 0.155904
Eisosomes 0.000275 0.000168 0.000107 0.000136 0.000185 0.000133 0.000325 0.000205 0.000233 0.000147 0.000114 0.000142
Endoplasmic Reticulum 0.001315 0.000829 0.006113 0.005987 0.001601 0.000735 0.005313 0.005998 0.001449 0.000771 0.006086 0.005988
Endosome 0.085672 0.103016 0.019956 0.030531 0.070791 0.116793 0.072695 0.063281 0.078711 0.111444 0.021727 0.033321
Golgi 0.109031 0.167740* 0.005631 0.004419 0.121312* 0.119928* 0.007573 0.003848 0.114776* 0.138489* 0.005696 0.004370
Lipid Particles 0.017117 0.006919 0.008809 0.009962 0.019931 0.019141 0.027183 0.012059 0.018433 0.014396 0.009426 0.010141
Mitochondria 0.451453* 0.342942* 0.024338 0.021435 0.363831* 0.403613* 0.005016 0.006238 0.410467* 0.380060* 0.023689 0.020140
Mitotic Spindle 0.004579 0.028556 0.049834 0.034384 0.008649 0.014345 0.012955 0.008632 0.006483 0.019862 0.048595 0.032190
None 0.000484 0.001002 0.001994 0.001749 0.001291 0.001095 0.003732 0.003232 0.000862 0.001059 0.002052 0.001875
Nuclear Periphery 0.000224 0.000228 0.003725 0.003147 0.000461 0.000318 0.001552 0.001187 0.000335 0.000283 0.003652 0.002980
Nuclear Periphery Foci 0.000236 0.000296 0.001848 0.001534 0.000704 0.000390 0.005780 0.003905 0.000455 0.000353 0.001980 0.001736
Nucleolus 0.000828 0.001195 0.004182 0.004936 0.002844 0.002550 0.008454 0.004861 0.001771 0.002024 0.004326 0.004929
Nucleus 0.005555 0.004932 0.240682* 0.230906* 0.011537 0.007254 0.199338* 0.196041* 0.008353 0.006353 0.239294* 0.227937*
Peroxisomes 0.050173 0.079620 0.035421 0.037757 0.054653 0.049423 0.110324* 0.057700 0.052269 0.061146 0.037936 0.039456
Vacuole 0.021970 0.025035 0.089261 0.137885 0.025622 0.041599 0.111925 0.120111 0.023678 0.035169 0.090022 0.136371
Vacuole Periphery 0.013854 0.015195 0.003155 0.004065 0.014436 0.018329 0.005114 0.002187 0.014126 0.017113 0.003221 0.003905

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.13 -3.14 -3.67 -2.91 -0.86 0.68 -3.18 -4.17 -4.29 0.65 -0.01 -5.18 -5.61 -5.86 -0.85
Bud Neck -0.35 -10.03 -10.11 -7.71 -1.09 -0.18 -0.07 0.32 0.42 0.23 -0.30 -11.21 -10.79 -9.72 -0.54
Bud Site -5.98 -9.47 -9.07 -0.60 1.10 0.07 1.07 -3.02 -3.04 -3.33 -4.44 -9.07 -9.08 -4.77 0.99
Cell Periphery -0.22 4.99 5.26 3.78 0.43 -0.38 -4.00 -2.95 -2.32 2.12 0.39 4.52 4.98 3.67 0.28
Cytoplasm 3.36 -42.15 -35.62 -36.80 5.07 5.14 -5.57 -11.36 -12.36 -2.51 6.08 -42.99 -37.70 -40.42 4.95
Cytoplasmic Foci 3.38 6.53 4.70 0.84 -1.76 12.88 0.33 -0.30 -5.44 -0.46 11.25 12.56 9.36 -0.77 -2.70
Eisosomes 5.47 10.02 8.14 2.59 -4.43 4.44 -4.15 -1.59 -4.64 3.14 7.61 11.01 8.10 0.44 -4.28
Endoplasmic Reticulum 1.67 -12.16 -9.46 -11.95 1.20 3.75 -3.44 -4.15 -4.96 -0.40 3.62 -13.59 -10.66 -13.15 1.12
Endosome -3.11 20.51 16.92 15.56 -5.76 -9.41 0.39 1.29 5.31 0.58 -8.85 24.52 18.28 23.63 -5.63
Golgi -7.95 27.43 27.91 25.87 1.55 0.26 25.69 28.52 25.96 1.56 -5.11 38.97 39.90 36.41 1.75
Lipid Particles 5.98 6.24 5.62 -2.03 -1.06 0.26 -1.60 3.28 2.83 3.45 2.59 8.16 7.83 3.61 -0.58
Mitochondria 8.39 53.41* 53.96* 30.88* 1.71 -3.34 43.53* 41.27* 44.58* -0.31 3.58 65.85* 66.91* 54.07* 2.10
Mitotic Spindle -7.66 -14.45 -10.87 -1.41 3.48 -3.33 -0.48 0.27 2.06 0.56 -8.44 -13.81 -10.11 -4.19 3.94
None -1.26 -9.11 -6.72 -1.94 1.29 0.34 -1.17 -1.94 -2.03 0.71 -0.69 -5.39 -4.66 -2.52 1.10
Nuclear Periphery -0.06 -11.70 -14.49 -13.67 1.56 1.68 -3.76 -3.50 -4.42 1.24 0.94 -11.43 -14.10 -14.35 1.93
Nuclear Periphery Foci -0.68 -8.12 -10.10 -9.72 1.38 1.48 -3.72 -4.02 -4.46 1.10 1.02 -7.31 -8.37 -10.30 1.04
Nucleolus -1.27 -8.06 -9.94 -8.46 -1.21 0.21 -1.42 -1.75 -1.85 0.87 -0.66 -5.34 -6.93 -5.49 -0.94
Nucleus 0.25 -43.10 -38.41 -38.15 2.59 2.54 -5.84 -10.86 -11.11 -0.30 1.77 -42.88 -39.42 -39.67 2.68
Peroxisomes -5.44 5.21 4.35 8.15 -0.87 1.28 -2.27 -0.20 -0.78 2.07 -2.89 5.75 5.23 7.11 -0.60
Vacuole -1.51 -24.67 -30.25 -28.12 -11.09 -5.45 -5.34 -8.77 -7.32 0.33 -5.96 -25.34 -31.41 -27.28 -10.81
Vacuole Periphery -0.88 13.22 12.52 8.80 -1.66 -2.93 4.58 13.05 14.94 1.11 -3.02 17.17 16.92 16.29 -1.28
Standard name
Human Ortholog
Description Mitochondrial inner membrane magnesium transporter; involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
Localization
Cell Percentages mitochondrion (88%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mfm1

Mfm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mfm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available