Standard name | |
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Human Ortholog | |
Description | Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 14 | 2 | 8 | 3 | 3 | 1 | 1 | 3 | 1 | 0 | 2 | 2 | 0 | 0 | 6 | 1 | 5 | 4 | 2 | 2 | 0 | 0 | 4 |
Bud | 5 | 3 | 1 | 2 | 0 | 1 | 4 | 1 | 2 | 5 | 6 | 8 | 0 | 1 | 0 | 1 | 0 | 0 | 5 | 4 | 1 | 0 | 1 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 1 | 1 | 13 | 16 | 5 | 0 | 0 | 3 |
Bud Site | 3 | 6 | 2 | 0 | 1 | 1 | 0 | 0 | 3 | 5 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 4 | 0 | 4 | 3 | 1 | 5 | 2 | 6 | 4 | 4 | 6 | 11 | 9 | 22 | 25 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 116 | 174 | 67 | 89 | 96 | 136 | 141 | 176 | 106 | 112 | 64 | 87 | 224 | 485 | 405 | 631 | 391 | 338 | 139 | 164 | 72 | 78 | 193 | 242 |
Endoplasmic Reticulum | 0 | 4 | 0 | 2 | 1 | 2 | 0 | 5 | 2 | 3 | 1 | 7 | 0 | 2 | 1 | 36 | 31 | 24 | 1 | 0 | 3 | 0 | 0 | 2 |
Endosome | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 7 | 5 | 3 | 3 | 4 | 1 | 1 | 2 | 3 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 8 | 1 | 2 | 9 | 10 | 47 | 69 | 85 | 129 | 116 | 97 | 135 | 0 | 1 | 0 | 6 | 3 | 2 | 3 | 3 | 1 | 2 | 3 | 9 |
Nucleus | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 4 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 5 | 4 | 6 | 2 | 9 | 0 | 3 | 3 | 4 | 0 | 2 | 0 | 0 | 0 | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 3 | 0 | 0 | 0 | 2 | 4 | 3 | 6 | 1 | 2 | 0 | 0 | 0 | 15 | 12 | 8 | 4 | 5 | 6 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 3 | 0 | 1 | 1 | 5 | 0 | 0 | 0 | 5 | 7 | 3 | 0 | 3 | 0 | 0 | 2 | 6 |
Unique Cell Count | 130 | 195 | 73 | 103 | 111 | 168 | 188 | 245 | 206 | 196 | 140 | 196 | 227 | 487 | 407 | 665 | 416 | 359 | 182 | 211 | 98 | 86 | 209 | 290 |
Labelled Cell Count | 146 | 210 | 81 | 122 | 116 | 208 | 226 | 286 | 256 | 261 | 180 | 254 | 232 | 496 | 419 | 728 | 481 | 416 | 182 | 211 | 98 | 86 | 209 | 290 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 5.6 | 5.2 | 4.5 | 4.7 | 4.1 | 3.8 | 4.1 | 4.0 | 4.0 | 3.8 | 3.6 | 5.9 | 6.1 | 5.9 | 6.0 | 7.5 | 7.5 | 4.6 | 5.4 | 6.1 |
Std Deviation (1e-4) | 0.5 | 0.7 | 1.9 | 0.7 | 0.9 | 1.4 | 1.1 | 1.0 | 1.2 | 1.5 | 0.8 | 0.8 | 1.0 | 1.8 | 1.5 | 1.4 | 1.5 | 1.7 | 1.0 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.22 | -0.16 | -0.34 | -0.45 | -0.36 | -0.4 | -0.4 | -0.48 | -0.54 | 0.17 | 0.22 | 0.17 | 0.21 | 0.53 | 0.52 | -0.19 | 0.04 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.1 | -1.1 | -2.1 | -3.0 | -3.5 | -6.1 | -5.4 | -6.6 | -7.0 | 3.0 | 5.2 | 4.7 | 1.1 | 0.7 | 0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.204 | 1.8558 | 1.704 | 1.3845 | 1.5756 | 1.737 | 3.8698 | 4.034 | 4.0923 | 4.2172 | 3.7767 | 4.0736 | 3.9612 | 3.704 | 4.4539 | 4.4068 | 4.5222 | 3.7762 |
Actin | 0.0756 | 0.0053 | 0.0131 | 0.0003 | 0.044 | 0.0129 | 0.0105 | 0.0035 | 0.0094 | 0.0075 | 0.0004 | 0.0148 | 0.0094 | 0.0002 | 0.0165 | 0.0013 | 0.0001 | 0.0042 |
Bud | 0.0029 | 0.0015 | 0.001 | 0.0004 | 0.0002 | 0.0012 | 0.0021 | 0.0049 | 0.0028 | 0.0013 | 0.0001 | 0.0026 | 0.0008 | 0 | 0.0013 | 0 | 0 | 0.0001 |
Bud Neck | 0.0214 | 0.0092 | 0.0007 | 0.0003 | 0.0005 | 0.1678 | 0.0159 | 0.0011 | 0.0007 | 0.0129 | 0.0001 | 0.1792 | 0.0005 | 0 | 0.0081 | 0.0001 | 0 | 0.1356 |
Bud Periphery | 0.004 | 0.002 | 0.0011 | 0.0007 | 0.0002 | 0.0011 | 0.0033 | 0.0075 | 0.0044 | 0.0027 | 0.0001 | 0.0072 | 0.0033 | 0 | 0.0018 | 0 | 0 | 0.0001 |
Bud Site | 0.0163 | 0.0268 | 0.0038 | 0.0004 | 0.0003 | 0.0027 | 0.0261 | 0.0395 | 0.0061 | 0.0138 | 0.0001 | 0.0035 | 0.0009 | 0.0002 | 0.0137 | 0 | 0 | 0.0003 |
Cell Periphery | 0.0015 | 0.0007 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0016 | 0.0005 | 0.0003 | 0.0007 | 0.0001 | 0.0009 | 0.0004 | 0.0002 | 0.0008 | 0.0001 | 0 | 0.0002 |
Cytoplasm | 0.2451 | 0.3992 | 0.3823 | 0.366 | 0.4614 | 0.3491 | 0.2971 | 0.4126 | 0.3766 | 0.3314 | 0.3328 | 0.4313 | 0.2246 | 0.4672 | 0.1997 | 0.4324 | 0.3514 | 0.3954 |
Cytoplasmic Foci | 0.0485 | 0.0128 | 0.0109 | 0.0454 | 0.0239 | 0.012 | 0.0343 | 0.0122 | 0.0129 | 0.0049 | 0.004 | 0.0058 | 0.0521 | 0.0031 | 0.0233 | 0.0035 | 0.0036 | 0.0099 |
Eisosomes | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0096 | 0.0043 | 0.0062 | 0.0037 | 0.0026 | 0.0016 | 0.0034 | 0.0015 | 0.0017 | 0.0048 | 0.0016 | 0.0011 | 0.0053 | 0.0021 | 0.0035 | 0.0025 | 0.0004 | 0.0025 |
Endosome | 0.0275 | 0.0058 | 0.0069 | 0.0235 | 0.0176 | 0.0012 | 0.0175 | 0.0023 | 0.0029 | 0.0028 | 0.0015 | 0.0012 | 0.0271 | 0.0008 | 0.0099 | 0.0008 | 0.0007 | 0.0065 |
Golgi | 0.0092 | 0.0013 | 0.0012 | 0.0014 | 0.0029 | 0.0003 | 0.0067 | 0.0006 | 0.0019 | 0.0007 | 0.0001 | 0.0012 | 0.0031 | 0.0001 | 0.017 | 0.0001 | 0 | 0.0045 |
Lipid Particles | 0.0096 | 0.0016 | 0.0019 | 0.0029 | 0.0043 | 0.0003 | 0.0072 | 0.0011 | 0.0015 | 0.0005 | 0.0001 | 0.0007 | 0.0058 | 0.0002 | 0.0282 | 0.0002 | 0.0002 | 0.0021 |
Mitochondria | 0.0028 | 0.0033 | 0.0006 | 0.0045 | 0.0009 | 0.0003 | 0.0034 | 0.0099 | 0.0083 | 0.0005 | 0.0002 | 0.0053 | 0.0099 | 0.0001 | 0.0028 | 0.0001 | 0 | 0.0011 |
None | 0.4905 | 0.5189 | 0.5532 | 0.5382 | 0.4173 | 0.4453 | 0.5473 | 0.493 | 0.5483 | 0.6009 | 0.6567 | 0.3339 | 0.4637 | 0.524 | 0.6615 | 0.5571 | 0.6419 | 0.4322 |
Nuclear Periphery | 0.0122 | 0.0008 | 0.0045 | 0.001 | 0.0014 | 0.0003 | 0.0018 | 0.0027 | 0.0011 | 0.002 | 0.0004 | 0.0025 | 0.0782 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0003 |
Nucleolus | 0.0008 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0019 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.002 | 0.0001 | 0.0003 | 0 | 0 | 0 |
Nucleus | 0.0035 | 0.0012 | 0.0034 | 0.0013 | 0.0011 | 0.0009 | 0.0014 | 0.0017 | 0.0054 | 0.0035 | 0.0005 | 0.0015 | 0.037 | 0.0005 | 0.0007 | 0.0004 | 0.0003 | 0.0004 |
Peroxisomes | 0.008 | 0.0019 | 0.0009 | 0.0008 | 0.0091 | 0.0006 | 0.0102 | 0.0005 | 0.0135 | 0.0004 | 0.0001 | 0.0053 | 0.0181 | 0 | 0.0074 | 0.0001 | 0.0001 | 0.0039 |
Punctate Nuclear | 0.0073 | 0.0011 | 0.0053 | 0.0013 | 0.0097 | 0.0008 | 0.0052 | 0.0026 | 0.001 | 0.001 | 0.0004 | 0.0012 | 0.0078 | 0.0002 | 0.001 | 0.0003 | 0.0002 | 0.0004 |
Vacuole | 0.002 | 0.0017 | 0.0016 | 0.0064 | 0.0013 | 0.0008 | 0.002 | 0.0014 | 0.001 | 0.0065 | 0.0005 | 0.0004 | 0.0157 | 0.0006 | 0.0011 | 0.0003 | 0.0005 | 0.0004 |
Vacuole Periphery | 0.0009 | 0.0002 | 0.0003 | 0.0008 | 0.0005 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.034 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.3444 | 13.0147 | 13.7238 | 23.5436 | 12.9152 | 11.4874 | 15.2369 | 14.0235 | 15.9823 | 22.7725 |
Translational Efficiency | 0.4929 | 0.352 | 0.28 | 0.1754 | 0.3026 | 0.4065 | 0.2588 | 0.3764 | 0.239 | 0.2257 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1270 | 894 | 282 | 1406 | 1439 | 1491 | 1635 | 2043 | 2709 | 2385 | 1917 | 3449 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 629.62 | 782.44 | 886.48 | 873.72 | 735.65 | 730.08 | 873.90 | 877.09 | 685.94 | 749.71 | 875.75 | 875.72 |
Standard Deviation | 77.26 | 112.18 | 98.94 | 126.56 | 86.66 | 98.13 | 133.38 | 122.93 | 97.92 | 106.67 | 128.97 | 124.43 |
Intensity Change Log 2 | — | 0.313499 | 0.493607 | 0.472690 | — | -0.010965 | 0.248449 | 0.253705 | — | 0.147768 | 0.366701 | 0.358836 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000051 | 0.000811 | 0.000552 | 0.001273 | 0.000053 | 0.001297 | 0.000816 | 0.001724 | 0.000052 | 0.001115 | 0.000777 | 0.001540 |
Bud Neck | 0.082498 | 0.038298 | 0.043108 | 0.029574 | 0.072146 | 0.076896 | 0.041692 | 0.041307 | 0.076999 | 0.062428 | 0.041900 | 0.036524 |
Bud Site | 0.019545 | 0.041239 | 0.044920 | 0.073946 | 0.020106 | 0.042584 | 0.063236 | 0.078932 | 0.019843 | 0.042080 | 0.060542 | 0.076900 |
Cell Periphery | 0.000198 | 0.000107 | 0.000212 | 0.000177 | 0.000172 | 0.000543 | 0.000546 | 0.000159 | 0.000184 | 0.000379 | 0.000497 | 0.000166 |
Cytoplasm | 0.594451* | 0.526926* | 0.363627* | 0.621220* | 0.624137* | 0.313313* | 0.564354* | 0.590974* | 0.610220* | 0.393384* | 0.534826* | 0.603304* |
Cytoplasmic Foci | 0.114017 | 0.181836* | 0.023938 | 0.020650 | 0.119043 | 0.192553* | 0.019634 | 0.021507 | 0.116687 | 0.188535* | 0.020268 | 0.021158 |
Eisosomes | 0.000117 | 0.000080 | 0.000093 | 0.000038 | 0.000090 | 0.000148 | 0.000045 | 0.000040 | 0.000103 | 0.000123 | 0.000052 | 0.000039 |
Endoplasmic Reticulum | 0.000486 | 0.000586 | 0.004577 | 0.001448 | 0.000668 | 0.000899 | 0.030269 | 0.000955 | 0.000583 | 0.000782 | 0.026490 | 0.001156 |
Endosome | 0.005631 | 0.024158 | 0.006951 | 0.004299 | 0.007268 | 0.065720 | 0.003713 | 0.005495 | 0.006501 | 0.050141 | 0.004189 | 0.005007 |
Golgi | 0.000687 | 0.004495 | 0.000174 | 0.001730 | 0.000687 | 0.010109 | 0.000798 | 0.003026 | 0.000687 | 0.008005 | 0.000706 | 0.002498 |
Lipid Particles | 0.002505 | 0.002239 | 0.002725 | 0.000590 | 0.002666 | 0.005104 | 0.000871 | 0.000512 | 0.002590 | 0.004030 | 0.001143 | 0.000544 |
Mitochondria | 0.000869 | 0.003289 | 0.000413 | 0.001680 | 0.000526 | 0.014336 | 0.000497 | 0.001699 | 0.000687 | 0.010195 | 0.000484 | 0.001691 |
Mitotic Spindle | 0.000903 | 0.004995 | 0.003131 | 0.029697 | 0.000067 | 0.013724 | 0.007729 | 0.052050 | 0.000459 | 0.010452 | 0.007053 | 0.042937 |
None | 0.005861 | 0.001454 | 0.003308 | 0.004852 | 0.006286 | 0.002329 | 0.002687 | 0.003316 | 0.006087 | 0.002001 | 0.002778 | 0.003942 |
Nuclear Periphery | 0.000201 | 0.000195 | 0.001904 | 0.000414 | 0.000211 | 0.000338 | 0.001048 | 0.000457 | 0.000207 | 0.000284 | 0.001174 | 0.000440 |
Nuclear Periphery Foci | 0.000494 | 0.000708 | 0.002713 | 0.001011 | 0.000525 | 0.000217 | 0.002331 | 0.000697 | 0.000511 | 0.000401 | 0.002387 | 0.000825 |
Nucleolus | 0.000586 | 0.000635 | 0.000891 | 0.000491 | 0.000766 | 0.001430 | 0.000285 | 0.000488 | 0.000682 | 0.001132 | 0.000374 | 0.000489 |
Nucleus | 0.138086* | 0.036347 | 0.330767* | 0.094779 | 0.104259 | 0.056412 | 0.146944* | 0.057711 | 0.120117 | 0.048891 | 0.173985* | 0.072822 |
Peroxisomes | 0.001221 | 0.011183 | 0.001760 | 0.002564 | 0.000952 | 0.012363 | 0.001772 | 0.002625 | 0.001078 | 0.011921 | 0.001770 | 0.002600 |
Vacuole | 0.031175 | 0.118230 | 0.163828 | 0.107996 | 0.038911 | 0.183243 | 0.110011 | 0.134484 | 0.035284 | 0.158873 | 0.117928 | 0.123686 |
Vacuole Periphery | 0.000420 | 0.002189 | 0.000408 | 0.001572 | 0.000462 | 0.006443 | 0.000722 | 0.001843 | 0.000442 | 0.004849 | 0.000675 | 0.001732 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.42 | -7.47 | -5.19 | -0.58 | -2.01 | -2.32 | -6.79 | -3.00 | 0.73 | -0.61 | -3.06 | -7.71 | -4.84 | 0.55 | -1.18 |
Bud Neck | 6.22 | 6.61 | 9.94 | 4.89 | 1.43 | -0.58 | 6.95 | 7.98 | 11.15 | 1.68 | 2.97 | 10.09 | 12.51 | 12.18 | 2.90 |
Bud Site | -5.05 | -0.89 | -5.27 | 0.10 | -2.26 | -6.84 | -5.38 | -5.93 | 0.71 | -0.01 | -8.59 | -5.73 | -7.95 | 0.65 | -0.52 |
Cell Periphery | 7.06 | 0.48 | 4.85 | -2.39 | 3.70 | -1.16 | -3.07 | 6.02 | 1.38 | 4.24 | -0.96 | -2.88 | 7.77 | 1.32 | 4.29 |
Cytoplasm | 5.61 | 16.75 | 6.51 | -0.02 | -12.52 | 30.59 | 12.38 | 10.93 | -20.42 | -1.83 | 26.61 | 16.27 | 12.39 | -15.66 | -5.67 |
Cytoplasmic Foci | -8.65 | 23.39 | 24.19 | 24.64 | -1.51 | -11.76 | 27.15 | 27.24 | 33.68 | -0.13 | -14.79 | 36.26 | 36.50 | 41.69 | -0.95 |
Eisosomes | 5.22 | 2.03 | 11.63 | 7.02 | 7.75 | -5.92 | 7.53 | 10.11 | 12.89 | 4.76 | -2.77 | 10.48 | 15.50 | 14.33 | 7.13 |
Endoplasmic Reticulum | -0.71 | -10.64 | -3.39 | -3.18 | 8.61 | -1.29 | -5.74 | -1.41 | -0.25 | 5.64 | -1.62 | -6.16 | -3.44 | -2.24 | 5.92 |
Endosome | -9.29 | -0.11 | 3.98 | 11.48 | 4.74 | -21.48 | 7.24 | 6.84 | 23.37 | -0.47 | -22.88 | 6.43 | 7.49 | 25.29 | 1.31 |
Golgi | -6.32 | 5.54 | -0.04 | 5.79 | -2.11 | -10.19 | 1.18 | -1.10 | 8.64 | -1.52 | -11.68 | 1.61 | -1.03 | 10.13 | -1.77 |
Lipid Particles | 0.54 | -0.80 | 4.76 | 3.32 | 6.20 | -5.19 | 6.88 | 9.85 | 10.21 | 3.72 | -4.12 | 5.83 | 9.69 | 10.51 | 5.24 |
Mitochondria | -2.17 | 2.65 | -0.25 | 2.10 | -2.50 | -8.84 | 0.30 | -2.13 | 8.53 | -2.36 | -8.95 | 2.13 | -1.63 | 8.67 | -3.23 |
Mitotic Spindle | -2.53 | -2.66 | -6.77 | -4.74 | -3.74 | -6.69 | -6.09 | -10.74 | -4.78 | -6.48 | -7.00 | -6.16 | -12.60 | -6.24 | -6.84 |
None | 4.29 | 1.70 | 1.39 | -5.36 | -0.53 | 3.69 | 4.28 | 3.66 | -1.13 | -1.29 | 5.24 | 4.81 | 3.58 | -3.16 | -2.34 |
Nuclear Periphery | -0.19 | -15.27 | -12.96 | -12.83 | 10.14 | -2.48 | -10.15 | -16.65 | -11.29 | 2.51 | -2.25 | -13.35 | -20.95 | -16.06 | 5.04 |
Nuclear Periphery Foci | -0.98 | -6.27 | -3.22 | -3.40 | 4.92 | 6.99 | -7.23 | -3.54 | -6.01 | 4.58 | 0.67 | -7.83 | -4.35 | -7.04 | 5.39 |
Nucleolus | -0.43 | -1.24 | 1.12 | 1.39 | 1.70 | -2.49 | 2.12 | 1.28 | 4.48 | -1.71 | -2.80 | 2.13 | 1.61 | 4.69 | -0.74 |
Nucleus | 20.20 | -14.12 | -0.80 | -22.00 | 13.87 | 10.40 | -14.96 | 0.28 | -10.67 | 15.66 | 20.13 | -17.24 | 0.22 | -21.09 | 17.76 |
Peroxisomes | -6.99 | 0.86 | -1.47 | 6.51 | -1.85 | -13.48 | -1.69 | -2.02 | 12.67 | -0.41 | -14.54 | -0.91 | -2.35 | 13.65 | -1.23 |
Vacuole | -17.06 | -13.61 | -27.71 | -10.30 | -0.65 | -25.23 | -25.59 | -34.46 | -8.16 | -8.51 | -30.40 | -29.55 | -44.26 | -12.30 | -7.84 |
Vacuole Periphery | -3.74 | 1.78 | -1.05 | 3.01 | -1.70 | -10.57 | 0.44 | -1.15 | 8.89 | -1.24 | -11.10 | 0.48 | -1.50 | 9.38 | -1.54 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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