Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.99 0.99 0.92 0.87 – 0.8 0.81 0.71 0.72 0.99 0.98 1.0 0.96 0.98 0.97 0.95 0.92 0.95 0.96 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0 – 0.2 0.16 0.26 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.16 – 0.12 0.12 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 1 2 0
Bud 0 0 0 0 0 6 0 – 2 4 4 2 3 0 0 0 0 0 3 0 1 0 4 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 6 1 3 1 5 – 3 7 4 2 1 1 3 5 2 9 0 0 0 0 0 0
Cytoplasm 316 157 268 285 347 286 394 – 288 268 189 183 260 449 492 204 158 134 308 155 256 183 431 295
Endoplasmic Reticulum 2 1 1 0 0 1 1 – 0 1 1 0 0 1 0 7 5 6 0 0 2 2 3 1
Endosome 0 0 0 0 0 1 2 – 0 4 1 0 0 0 0 1 0 0 1 0 0 0 0 1
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 2 1 0 1 1 2 0 1 0 1 0
Mitochondria 2 1 0 1 2 42 15 – 70 54 70 66 1 0 1 1 1 2 1 0 2 0 3 3
Nucleus 0 2 0 0 1 2 11 – 5 8 9 5 0 1 0 5 1 0 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 – 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 1 1 3 0
Vac/Vac Membrane 0 0 1 4 5 6 71 – 44 41 31 34 2 5 3 6 4 3 0 5 0 0 1 0
Unique Cell Count 321 159 270 288 350 311 455 358 332 265 253 263 457 494 212 161 138 325 169 271 191 459 317
Labelled Cell Count 322 161 276 291 358 346 500 413 387 311 294 267 459 500 229 172 155 325 169 271 191 459 317


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.3 6.9 6.3 7.0 4.9 6.3 – 5.7 6.0 5.6 5.9 6.6 7.2 7.6 8.0 8.1 8.5 5.9 6.4 6.9
Std Deviation (1e-4) 0.8 1.2 1.2 1.4 1.6 1.2 1.5 – 1.2 1.1 1.1 1.1 1.1 1.5 1.8 1.4 1.5 2.8 1.2 1.6 1.9
Intensity Change (Log2) – – – -0.12 0.03 -0.5 -0.12 – -0.26 -0.2 -0.3 -0.22 -0.06 0.06 0.15 0.21 0.22 0.3 -0.22 -0.1 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 -0.1 0 0 0 0 0 0 0 2.2
Cytoplasm -0.4 -0.2 -4.2 -5.9 – -7.3 -7.2 -9.2 -8.9 -0.5 -1.1 0.6 -2.3 -1.1 -1.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.3 3.0 – 7.7 6.9 9.1 9.0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 6.6 – 5.7 5.7 5.5 6.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3471 0.0359 -0.1314 -0.2634 0.2478 0.0611 1.296 1.594 1.265 1.1553 1.2563 1.092 3.4371 4.0643 3.6806 3.5426 3.7923 3.5782
Actin 0.0535 0.0017 0.016 0.0229 0.01 0.0049 0.0494 0.0014 0.0098 0.0048 0.0446 0.0041 0.0689 0.0001 0.0003 0.0004 0.0078 0.0168
Bud 0.0015 0.0032 0.0002 0.0001 0.0001 0.0003 0.0004 0.0047 0.0026 0.0009 0.0019 0.0001 0.0013 0 0.0001 0.0002 0.0003 0.0005
Bud Neck 0.0021 0.0001 0.0003 0.0003 0.0002 0.0005 0.004 0.0003 0.0002 0.0035 0.0008 0.0004 0.0006 0 0.0001 0.074 0.0001 0.0007
Bud Periphery 0.0016 0.0028 0.0002 0.0002 0.0002 0.0005 0.0006 0.003 0.0022 0.002 0.0018 0.0001 0.0014 0 0 0.0001 0.0005 0.0007
Bud Site 0.0345 0.0029 0.0007 0.0003 0.0001 0.0003 0.0014 0.016 0.0022 0.0067 0.0019 0.0001 0.0047 0.0001 0.0009 0.001 0.0001 0.0007
Cell Periphery 0.0004 0.0002 0.0001 0.0001 0 0.0001 0.0002 0.0003 0.0001 0.0006 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.1899 0.4274 0.4602 0.2307 0.5887 0.5856 0.51 0.643 0.6104 0.5481 0.5245 0.7365 0.3723 0.546 0.5122 0.639 0.5283 0.6169
Cytoplasmic Foci 0.0384 0.0069 0.007 0.0198 0.012 0.0092 0.0074 0.0047 0.0107 0.0355 0.0498 0.0043 0.0097 0.0016 0.0055 0.0085 0.0073 0.0094
Eisosomes 0.0005 0.0001 0.0004 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0005 0.0001 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0075 0.002 0.0031 0.0103 0.0026 0.0026 0.0036 0.0014 0.0028 0.0043 0.0019 0.0012 0.0027 0.0015 0.0017 0.002 0.0178 0.0017
Endosome 0.0712 0.0043 0.0058 0.0125 0.0117 0.0096 0.0113 0.0006 0.0075 0.008 0.0055 0.0039 0.0062 0.0003 0.0035 0.0037 0.0353 0.0034
Golgi 0.0178 0.0003 0.005 0.0491 0.0214 0.0076 0.0046 0.0001 0.0016 0.0034 0.0088 0.0043 0.0027 0.0001 0.0016 0.0003 0.007 0.0031
Lipid Particles 0.0332 0.006 0.0012 0.0373 0.0609 0.0022 0.0165 0.0002 0.0003 0.0035 0.0268 0.0012 0.0015 0.0001 0.0033 0 0.001 0.0021
Mitochondria 0.007 0.0002 0.0008 0.0096 0.0037 0.0184 0.0056 0.0002 0.0026 0.0017 0.0045 0.0016 0.0016 0.0001 0.0018 0.0004 0.0093 0.0014
None 0.4766 0.5384 0.4908 0.5613 0.2757 0.3511 0.3411 0.3208 0.3329 0.3616 0.3057 0.2398 0.5045 0.4487 0.4644 0.2656 0.3574 0.3384
Nuclear Periphery 0.0198 0.0007 0.0015 0.0029 0.0025 0.0019 0.0208 0.0005 0.0054 0.0015 0.0011 0.0003 0.0017 0.0003 0.0004 0.0008 0.0188 0.0005
Nucleolus 0.0013 0.0001 0.0006 0.0001 0.0001 0.0002 0.0007 0.0001 0.0004 0.0005 0.0017 0 0.0001 0 0.0001 0.0003 0.0001 0.0001
Nucleus 0.0078 0.0009 0.0019 0.0008 0.001 0.0013 0.0124 0.0011 0.0045 0.0029 0.0018 0.0012 0.0012 0.0005 0.0012 0.002 0.0023 0.0012
Peroxisomes 0.0148 0.0002 0.0006 0.0386 0.0054 0.0004 0.0037 0.0001 0.0003 0.0035 0.0091 0.0001 0.0158 0 0.0017 0.0001 0.0001 0.0011
Punctate Nuclear 0.0083 0.0011 0.0031 0.0014 0.0023 0.0011 0.0032 0.0008 0.0025 0.0015 0.0065 0.0003 0.0016 0.0002 0.0003 0.0007 0.0008 0.0008
Vacuole 0.0089 0.0006 0.0005 0.0011 0.0006 0.001 0.0016 0.0004 0.0006 0.0043 0.0007 0.0003 0.0007 0.0002 0.0005 0.0006 0.002 0.0003
Vacuole Periphery 0.0032 0.0001 0.0001 0.0004 0.0005 0.0013 0.0015 0.0001 0.0003 0.0009 0.0003 0.0001 0.0002 0 0.0001 0.0002 0.0033 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.7101 11.5655 12.2602 14.6953 19.1707 11.0834 20.0467 15.2215 20.0108 24.7621
Translational Efficiency 1.1351 2.2461 1.1638 1.6729 1.7801 2.709 1.2296 2.3255 1.5158 1.4013

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2007 1557 1821 22 1802 2062 771 1692 3809 3619 2592 1714

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 773.61 845.01 955.52 979.85 747.59 872.18 903.95 1037.53 761.30 860.49 940.18 1036.79
Standard Deviation 91.64 119.19 114.56 104.47 94.24 145.04 114.34 173.63 93.78 135.20 116.90 173.03
Intensity Change Log 2 — 0.127362 0.304680 0.340954 — 0.222379 0.273996 0.472834 — 0.174840 0.289682 0.407273

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000081 0.000224 0.000227 0.000226 0.000101 0.000611 0.000545 0.001953 0.000090 0.000445 0.000322 0.001930
Bud Neck 0.007016 0.007647 0.005531 0.001143 0.004487 0.013543 0.002847 0.007652 0.005819 0.011006 0.004733 0.007569
Bud Site 0.002488 0.010085 0.006332 0.002528 0.003321 0.014272 0.009532 0.050371 0.002882 0.012470 0.007284 0.049757
Cell Periphery 0.000057 0.000084 0.000089 0.000089 0.000077 0.000086 0.000103 0.000139 0.000067 0.000085 0.000094 0.000139
Cytoplasm 0.646934* 0.673640* 0.551237* 0.701196* 0.683895* 0.657010* 0.605418* 0.594530* 0.664420* 0.664165* 0.567354* 0.595899*
Cytoplasmic Foci 0.134668 0.124523 0.007206 0.015823 0.128939 0.110439 0.025254 0.019690 0.131958 0.116499 0.012574 0.019640
Eisosomes 0.000052 0.000035 0.000017 0.000029 0.000049 0.000056 0.000036 0.000024 0.000050 0.000047 0.000023 0.000024
Endoplasmic Reticulum 0.000465 0.001034 0.001899 0.003634 0.000574 0.000373 0.001751 0.002300 0.000516 0.000657 0.001855 0.002317
Endosome 0.004764 0.007313 0.001211 0.001564 0.004962 0.007206 0.003501 0.011052 0.004857 0.007252 0.001892 0.010930
Golgi 0.001103 0.001602 0.000078 0.000075 0.001283 0.002126 0.003683 0.005688 0.001188 0.001901 0.001150 0.005616
Lipid Particles 0.001975 0.001367 0.000605 0.001329 0.002305 0.001407 0.001246 0.000821 0.002131 0.001390 0.000796 0.000827
Mitochondria 0.001920 0.001118 0.000551 0.000183 0.000908 0.002412 0.003207 0.004784 0.001441 0.001855 0.001341 0.004725
Mitotic Spindle 0.000922 0.002048 0.004898 0.003999 0.001585 0.008981 0.005993 0.072551 0.001236 0.005998 0.005224 0.071672
None 0.002498 0.004447 0.005107 0.002114 0.004994 0.003275 0.006500 0.009360 0.003679 0.003780 0.005521 0.009267
Nuclear Periphery 0.000240 0.000212 0.001117 0.001321 0.000170 0.000194 0.001004 0.000855 0.000207 0.000202 0.001083 0.000861
Nuclear Periphery Foci 0.000395 0.001001 0.001391 0.002629 0.000588 0.000288 0.001541 0.001423 0.000486 0.000595 0.001436 0.001438
Nucleolus 0.000476 0.000740 0.000147 0.000155 0.000540 0.000490 0.000224 0.000435 0.000506 0.000598 0.000170 0.000432
Nucleus 0.128126 0.096783 0.366269* 0.070131 0.118286 0.098798 0.194873* 0.095649 0.123470 0.097931 0.315287* 0.095322
Peroxisomes 0.001341 0.002558 0.000158 0.000108 0.001888 0.003728 0.000505 0.002069 0.001600 0.003224 0.000261 0.002044
Vacuole 0.064148 0.062459 0.045824 0.191637 0.040374 0.073905 0.131635 0.112941 0.052901 0.068980 0.071349 0.113951
Vacuole Periphery 0.000332 0.001081 0.000105 0.000087 0.000675 0.000798 0.000602 0.005713 0.000494 0.000920 0.000253 0.005641

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.26 -7.78 -2.55 -1.35 -0.85 -1.87 -3.61 -2.73 -0.32 -1.37 -2.30 -7.57 -2.80 -0.99 -1.73
Bud Neck -0.42 1.65 0.89 1.08 0.10 -8.27 2.10 0.02 8.80 -2.50 -6.38 1.68 2.38 8.92 0.05
Bud Site -8.64 -3.72 -1.65 1.46 -0.30 -8.74 -2.11 -8.15 -1.82 -5.07 -11.92 -4.07 -8.49 -2.85 -6.31
Cell Periphery -4.92 -8.02 -1.46 -0.61 -0.09 -1.36 -2.56 -0.33 0.77 1.54 -4.40 -7.81 -2.18 0.74 2.68
Cytoplasm -2.88 20.13 4.00 4.35 1.59 4.17 19.12 20.78 16.45 -3.09 0.77 28.84 20.66 19.47 -3.22
Cytoplasmic Foci 1.95 35.22 21.57 18.66 -1.02 3.88 28.78 31.68 29.79 2.45 4.40 46.76 45.37 39.56 -2.56
Eisosomes 6.04 10.86 -0.67 -1.82 -2.51 -1.87 1.06 9.90 8.94 8.99 1.43 10.66 13.20 9.26 3.07
Endoplasmic Reticulum -3.46 -16.84 -2.18 -1.88 -0.99 2.35 -12.18 -2.23 -2.98 4.53 -1.82 -20.36 -2.67 -2.07 5.21
Endosome -3.78 12.20 5.01 6.79 -0.86 -3.35 5.91 2.83 5.40 -1.52 -5.09 13.04 3.14 6.32 -2.87
Golgi -1.17 3.72 3.61 5.13 -1.35 -1.71 0.04 -0.15 1.29 -0.12 -2.10 2.74 -0.36 1.12 -2.11
Lipid Particles 3.45 7.60 0.33 -0.73 -1.67 2.12 3.16 4.88 1.85 4.47 3.13 7.02 7.67 2.88 1.82
Mitochondria 1.14 1.97 2.27 3.12 1.77 -2.33 -0.44 -1.93 1.31 -0.23 -0.75 1.43 -0.16 0.73 -1.97
Mitotic Spindle -1.60 -3.91 -1.61 -1.43 -0.94 -4.22 -2.71 -9.74 -6.78 -8.30 -4.62 -4.52 -10.02 -8.20 -8.55
None -1.89 -5.77 -0.70 0.47 0.75 1.89 0.01 -4.01 -5.67 -3.75 -0.06 -2.48 -6.55 -5.63 -5.41
Nuclear Periphery 0.79 -28.61 -3.72 -3.74 -1.89 -2.56 -20.78 -20.76 -20.16 2.10 -0.44 -36.05 -20.47 -20.36 1.95
Nuclear Periphery Foci -6.28 -10.72 -1.76 -1.26 -0.67 5.53 -9.62 -4.26 -6.69 4.17 -2.21 -13.43 -5.32 -4.17 4.49
Nucleolus -1.44 7.14 1.85 2.28 -1.54 0.20 2.39 1.40 2.54 -3.98 -0.93 4.95 2.23 3.06 -5.83
Nucleus 7.32 -40.42 -0.97 -2.03 8.36 4.57 -15.96 -10.90 -14.66 9.15 8.40 -42.77 -10.85 -16.35 28.70
Peroxisomes -3.75 10.65 10.66 7.97 0.19 -2.84 4.59 3.48 5.60 -1.34 -4.29 8.18 4.30 7.10 -2.02
Vacuole -0.24 -3.83 -5.33 -5.32 -5.10 -12.28 -20.35 -29.40 -22.04 -0.41 -8.36 -17.47 -27.93 -24.24 -14.91
Vacuole Periphery -3.46 9.97 6.18 4.43 -0.54 -0.41 1.63 -1.54 -1.43 -2.71 -2.55 3.00 -2.13 -1.13 -3.06
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene
Localization
Cell Percentages cytoplasm (88%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Htc1

Htc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Htc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available