ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.18 0.26 0.35 0.35 0.16 0.16 0.11 0.12 0 0.18 0.34 0.07 0.09 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.2 0 0 0 0.22 0.24 0.13 0.65 0.56 0.59 0.6 0 0 0 0.08 0.12 0.08 0 0 0 0 0 0.06
Nucleus 0.89 0.68 0.94 0.91 0.64 0.48 0.4 0.43 0.43 0.45 0.55 0.52 1.0 0.78 0.6 0.85 0.82 0.81 0.75 0.61 0.78 0.77 0.66 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.15 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.07 0 0 0.13 0.13 0.1 0.11 0.07 0.07 0 0.05 0 0 0.09 0 0.1 0.1 0.13 0.21 0.12 0.11 0.17 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud 0 0 0 0 0 0 0 0 7 3 4 4 0 0 0 0 0 0 0 2 3 0 1 3
Bud Neck 0 4 2 1 3 1 8 16 3 2 2 1 0 1 0 0 0 0 0 1 0 0 0 0
Bud Site 0 9 0 0 0 1 2 0 5 6 4 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 0 7 14 16 50 75 145 137 64 66 41 54 0 30 53 5 8 6 0 10 3 1 3 5
Endoplasmic Reticulum 0 1 0 0 0 1 0 0 0 0 0 1 0 0 1 2 1 4 0 4 0 0 0 0
Endosome 0 1 1 0 15 5 5 1 0 3 2 0 0 1 0 1 0 3 0 3 2 0 3 4
Golgi 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Mitochondria 18 46 3 8 6 64 101 52 257 238 229 265 0 0 0 6 11 8 0 7 6 0 1 10
Nucleus 67 156 324 208 173 140 166 171 171 190 214 231 16 129 94 62 77 80 81 164 294 29 94 84
Nuclear Periphery 0 2 0 0 0 0 0 1 1 3 0 1 0 0 0 0 0 2 0 2 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 1 3 5 7 0 5 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 35 11 3 10 9 11 17 4 9 4 7 0 1 3 0 1 0 3 4 5 0 4 7
Vac/Vac Membrane 4 16 5 2 36 39 43 45 27 30 15 20 0 6 14 2 9 10 13 55 46 4 24 46
Unique Cell Count 75 229 346 229 271 291 416 395 396 425 386 444 16 165 156 73 94 99 108 270 380 38 144 176
Labelled Cell Count 89 277 360 238 293 335 481 440 540 551 515 593 16 168 165 79 107 114 108 270 380 38 144 176


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.4 5.8 5.4 5.6 4.4 4.4 4.6 3.6 4.0 3.8 3.8 4.5 5.4 4.7 5.2 6.1 6.2 – 6.1 5.7
Std Deviation (1e-4) 0.6 18.6 0.9 1.4 1.3 0.9 1.4 1.0 0.6 1.2 0.6 0.6 0.5 1.1 0.7 1.1 1.1 1.3 – 1.6 1.3
Intensity Change (Log2) – – – -0.09 -0.04 -0.39 -0.39 -0.33 -0.66 -0.52 -0.59 -0.58 -0.34 -0.1 -0.31 -0.16 0.09 0.11 – 0.09 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 5.8 7.9 10.4 10.3 5.4 5.2 3.4 4.0 0 5.3 9.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -1.3 -9.3 -12.9 -15.4 -14.5 -14.6 -14.3 -11.7 -12.7 1.0 -5.2 -9.3 -2.5 -3.5 -3.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.4 0.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 5.9 5.9 5.0 5.4 3.6 3.7 2.0 2.4 0 0 4.1 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5298 3.8346 3.4759 3.797 3.107 3.511 1.1827 1.5005 1.3172 0.7852 0.6805 1.4664 -0.9541 -0.3821 -0.7288 -0.3635 -0.6312 -0.7307
Actin 0.0211 0 0.0063 0.0005 0.0055 0.0009 0.0024 0.0585 0.0032 0 0.0001 0.0044 0 0.0001 0.0002 0 0.0001 0
Bud 0.0004 0 0.0002 0.0001 0.0007 0.0001 0.0005 0.0008 0.0003 0.0001 0 0.0002 0 0.0002 0.0001 0 0.0001 0.0001
Bud Neck 0.0083 0.0003 0.0006 0.0003 0.0006 0.0013 0.0006 0.0003 0.0002 0.0003 0.0019 0.0137 0.0002 0.0002 0.0005 0.0001 0.001 0.0011
Bud Periphery 0.0016 0 0.0006 0.0004 0.0012 0.0006 0.0007 0.0009 0.001 0.0001 0.0001 0.0003 0 0.0002 0.0001 0 0.0002 0.0001
Bud Site 0.0085 0.0004 0.0011 0.0001 0.0008 0.0001 0.0112 0.0031 0.0007 0.0001 0.0002 0.0015 0.0001 0.0004 0.0028 0 0.0006 0.0001
Cell Periphery 0.0004 0 0.0002 0.0001 0.0002 0.0001 0.0011 0.0001 0.0001 0 0 0.0001 0 0 0 0 0.0001 0
Cytoplasm 0.0058 0.0107 0.0034 0.0061 0.0004 0.0012 0.0311 0.0003 0.0067 0.0008 0.0043 0.0018 0.0071 0.0292 0.0078 0.0003 0.014 0.0014
Cytoplasmic Foci 0.0144 0.0003 0.0074 0.0094 0.0016 0.0001 0.002 0.0012 0.0062 0 0.0001 0.0006 0.0001 0.0006 0.0017 0 0.0002 0
Eisosomes 0.0005 0 0.0001 0 0.0545 0 0.0001 0.0005 0.0001 0 0 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0024 0.0002 0.0006 0.0004 0.0004 0.0005 0.002 0.0002 0.0014 0 0.0001 0.0002 0.0002 0.0034 0.0002 0 0.0014 0.0001
Endosome 0.0199 0.0003 0.0031 0.0024 0.0015 0.0014 0.0032 0.0025 0.0089 0 0.0001 0.0018 0.0002 0.001 0.0007 0 0.0011 0.0001
Golgi 0.0045 0 0.0026 0.0005 0.0046 0.0007 0.0009 0.0084 0.0058 0 0 0.0035 0 0 0.0001 0 0 0
Lipid Particles 0.008 0.0001 0.0021 0.0046 0.0008 0.0006 0.0003 0.0004 0.0006 0 0 0.0006 0 0.0001 0.0002 0 0 0
Mitochondria 0.0036 0.0001 0.016 0.0024 0.0617 0.009 0.0012 0.0326 0.0106 0.0004 0.0003 0.0203 0.0002 0.0004 0.0002 0.0001 0.0006 0.0001
None 0.0209 0.0148 0.0143 0.0495 0.0171 0.001 0.0246 0.0001 0.0383 0.0001 0.0025 0.0006 0.0004 0.0088 0.012 0 0.0052 0.0001
Nuclear Periphery 0.011 0.0036 0.0091 0.0058 0.001 0.0067 0.0036 0.0005 0.0026 0.0028 0.0038 0.0013 0.0057 0.0192 0.0048 0.0005 0.02 0.0024
Nucleolus 0.0084 0.0104 0.0102 0.0075 0.0115 0.019 0.0047 0.0057 0.0089 0.008 0.0249 0.0143 0.0059 0.0081 0.0038 0.002 0.0301 0.0086
Nucleus 0.8293 0.9488 0.9075 0.8284 0.8317 0.9461 0.8998 0.882 0.8935 0.9865 0.9482 0.9174 0.9744 0.9208 0.9549 0.9966 0.9178 0.9842
Peroxisomes 0.004 0 0.0049 0.0298 0.0027 0.0002 0.0001 0.001 0.001 0 0 0.0002 0 0 0.0001 0 0 0
Punctate Nuclear 0.0173 0.0095 0.0072 0.0114 0.0011 0.0085 0.0072 0.0003 0.0094 0.0006 0.013 0.0168 0.0048 0.0038 0.0094 0.0005 0.0059 0.0015
Vacuole 0.0043 0.0004 0.0013 0.0109 0.0002 0.0009 0.0024 0.0003 0.0003 0.0001 0.0002 0.0002 0.0003 0.0026 0.0003 0 0.0012 0.0002
Vacuole Periphery 0.0053 0 0.0013 0.0296 0.0002 0.001 0.0004 0.0002 0.0002 0 0.0001 0.0003 0.0001 0.001 0.0001 0 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.7613 12.3017 20.7464 16.2971 13.7871 8.7523 16.1132 10.6845 9.561 19.2216
Translational Efficiency 1.0252 1.7423 0.7356 0.7988 1.126 1.1982 0.81 1.0951 0.9542 0.8501

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2280 1222 2000 820 1760 1878 1841 1455 4040 3100 3841 2275

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 708.87 688.06 799.93 891.07 655.22 790.65 799.04 878.14 685.50 750.21 799.50 882.80
Standard Deviation 91.17 102.42 120.77 111.90 75.38 98.87 133.42 119.97 88.74 112.12 126.99 117.29
Intensity Change Log 2 — -0.042987 0.174353 0.330018 — 0.271060 0.286288 0.422472 — 0.116400 0.229204 0.375166

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000080 0.000866 0.000499 0.001159 0.000156 0.000169 0.000744 0.001937 0.000113 0.000444 0.000616 0.001657
Bud Neck 0.003998 0.010089 0.015465 0.017311 0.002860 0.007765 0.014256 0.022167 0.003502 0.008681 0.014885 0.020417
Bud Site 0.000545 0.003885 0.004086 0.007760 0.001101 0.001869 0.003047 0.007296 0.000787 0.002663 0.003588 0.007463
Cell Periphery 0.000112 0.000216 0.000135 0.000143 0.000100 0.000125 0.000131 0.000142 0.000107 0.000161 0.000133 0.000142
Cytoplasm 0.068501 0.088536 0.013372 0.015166 0.068601 0.048023 0.012756 0.017705 0.068545 0.063993 0.013077 0.016790
Cytoplasmic Foci 0.007358 0.013395 0.000149 0.001992 0.008844 0.008717 0.000300 0.001560 0.008005 0.010561 0.000221 0.001716
Eisosomes 0.000016 0.000034 0.000018 0.000028 0.000023 0.000025 0.000023 0.000028 0.000019 0.000029 0.000020 0.000028
Endoplasmic Reticulum 0.001421 0.004962 0.003939 0.008869 0.001143 0.002377 0.005378 0.007567 0.001300 0.003396 0.004629 0.008036
Endosome 0.000690 0.002381 0.000393 0.001031 0.000467 0.002291 0.000418 0.001999 0.000593 0.002327 0.000405 0.001650
Golgi 0.000398 0.001403 0.000079 0.003662 0.000163 0.001420 0.000182 0.001449 0.000296 0.001414 0.000128 0.002246
Lipid Particles 0.001484 0.002358 0.000137 0.000310 0.001488 0.002541 0.000179 0.000282 0.001486 0.002469 0.000157 0.000292
Mitochondria 0.003229 0.005608 0.001495 0.003980 0.001615 0.007302 0.001253 0.003133 0.002525 0.006634 0.001379 0.003438
Mitotic Spindle 0.002813 0.005686 0.002408 0.014507 0.001247 0.004737 0.003851 0.013726 0.002130 0.005111 0.003099 0.014007
None 0.007338 0.022205 0.004128 0.004574 0.010016 0.009969 0.007132 0.006251 0.008505 0.014792 0.005568 0.005647
Nuclear Periphery 0.001919 0.001396 0.000768 0.001700 0.001591 0.002442 0.000603 0.002739 0.001776 0.002030 0.000689 0.002365
Nuclear Periphery Foci 0.000295 0.001226 0.000239 0.000629 0.000270 0.000396 0.000440 0.000545 0.000284 0.000723 0.000335 0.000575
Nucleolus 0.004276 0.013885 0.002803 0.004862 0.005523 0.008405 0.007021 0.003518 0.004819 0.010565 0.004824 0.004003
Nucleus 0.892930* 0.812936* 0.946787* 0.902996* 0.892540* 0.884636* 0.940072* 0.899379* 0.892760* 0.856373* 0.943568* 0.900683*
Peroxisomes 0.000796 0.003148 0.000091 0.000355 0.000762 0.001928 0.000217 0.000240 0.000781 0.002409 0.000151 0.000282
Vacuole 0.001525 0.004807 0.002303 0.007968 0.001336 0.003799 0.001841 0.006538 0.001443 0.004196 0.002082 0.007053
Vacuole Periphery 0.000279 0.000978 0.000706 0.000997 0.000152 0.001061 0.000157 0.001799 0.000224 0.001028 0.000443 0.001510

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.78 -9.35 -13.98 -2.38 -8.33 -0.38 -9.21 -2.39 -2.36 -1.32 -3.87 -13.28 -3.63 -2.73 -2.19
Bud Neck -6.17 -10.85 -9.61 -5.41 -1.60 -5.01 -13.44 -13.64 -9.47 -3.38 -7.32 -16.81 -16.42 -10.86 -3.79
Bud Site -5.58 -4.38 -4.11 -1.37 -1.57 -1.55 -2.71 -5.36 -4.71 -3.20 -5.48 -5.20 -7.07 -4.29 -3.27
Cell Periphery -4.64 -0.51 -1.99 3.33 -1.34 -1.72 -1.41 -1.99 -0.27 -0.67 -4.39 -1.27 -2.60 2.30 -1.30
Cytoplasm -3.63 17.76 15.54 14.26 -1.60 4.74 16.27 14.29 10.65 -2.66 1.21 24.11 21.08 17.53 -3.00
Cytoplasmic Foci -3.54 8.55 6.56 7.89 -1.57 0.23 8.36 7.25 7.47 -2.01 -2.35 11.94 9.86 10.86 -2.65
Eisosomes -9.18 -2.92 -11.91 -1.96 -10.53 -0.70 -0.75 -3.18 -3.79 -4.52 -6.26 -2.32 -10.10 -3.92 -10.87
Endoplasmic Reticulum -12.78 -13.12 -20.20 -11.56 -13.48 -6.86 -21.60 -22.17 -17.03 -7.67 -13.10 -23.71 -29.29 -20.06 -14.95
Endosome -3.92 2.42 -1.58 3.38 -4.56 -3.98 0.78 -2.55 1.25 -2.72 -5.36 2.46 -2.66 2.56 -3.46
Golgi -1.60 2.27 -1.11 -0.09 -1.45 -2.73 -0.02 -1.39 0.73 -1.37 -2.95 1.98 -1.64 0.46 -2.03
Lipid Particles -2.87 7.36 5.61 7.64 -7.22 -2.75 8.28 6.93 6.11 -4.19 -3.73 10.69 8.60 8.77 -7.47
Mitochondria -2.29 2.60 0.11 2.07 -1.79 -4.82 0.67 -2.41 3.68 -3.67 -4.84 2.69 -0.80 4.26 -3.60
Mitotic Spindle -2.47 -0.53 -4.17 -3.05 -3.96 -2.90 -2.22 -5.33 -3.55 -3.96 -3.45 -1.78 -6.56 -4.66 -5.66
None -7.38 4.43 3.48 9.42 -0.89 -0.02 3.40 4.27 4.05 0.80 -5.47 5.24 4.99 9.36 -0.02
Nuclear Periphery 1.92 5.37 -2.91 -4.41 -6.72 -1.60 1.95 -1.76 -0.61 -3.31 -1.03 4.36 -2.40 -1.68 -4.74
Nuclear Periphery Foci -5.90 0.64 -2.77 3.16 -3.11 -1.22 -1.80 -2.57 -1.96 -1.51 -5.38 -1.10 -3.37 0.06 -2.77
Nucleolus -7.66 2.06 0.90 7.52 -0.38 -3.24 -2.37 4.09 6.14 6.01 -7.90 -0.74 3.12 9.77 3.85
Nucleus 9.74 -10.24 0.99 -7.67 8.26 1.28 -8.48 -0.53 -1.73 7.30 7.30 -13.24 0.14 -6.47 11.02
Peroxisomes -4.03 4.18 3.16 5.30 -2.70 -3.37 4.52 4.28 5.40 -0.42 -5.14 5.79 4.89 7.53 -2.40
Vacuole -4.92 -3.08 -8.37 -6.15 -7.77 -4.82 -3.24 -8.08 -5.89 -7.34 -6.77 -4.30 -11.55 -8.42 -10.56
Vacuole Periphery -3.73 -0.77 -1.15 1.50 0.03 -3.64 0.06 -2.29 -0.19 -2.29 -4.69 -0.73 -2.42 0.26 -1.66
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YPL068C

YPL068C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl068c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available