Standard name
Human Ortholog
Description Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0.12 0.11 0.05 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.1 0 0 0 – 0 0 0 0 0.05 0.05 0.08 0.06 0.1 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.99 0.99 0.97 0.95 – 0.82 0.81 0.75 0.8 0.79 0.74 0.88 0.95 0.95 0.86 0.89 0.93 0.84 0.82 0.79 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.12 – 0.25 0.29 0.42 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 0 0 0 – 2 1 2 2 35 23 19 1 3 0 1 1 5 2 6 9
Bud 0 3 1 1 0 1 0 – 7 9 8 7 29 7 7 0 2 0 0 0 2 0 3 7
Bud Neck 0 0 4 0 4 3 2 – 5 0 3 6 0 2 0 1 1 0 1 1 4 5 7 8
Bud Site 0 0 1 0 0 0 0 – 1 0 1 0 6 0 1 0 0 0 – – – – – –
Cell Periphery 0 6 9 18 11 8 14 – 5 4 10 12 16 11 29 11 18 8 0 2 10 6 8 3
Cytoplasm 46 194 260 180 253 229 346 – 207 207 228 268 234 161 330 184 164 90 65 191 227 155 261 269
Endoplasmic Reticulum 1 1 2 0 0 0 3 – 2 2 3 1 2 0 0 17 7 5 0 0 1 2 7 4
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 2 3 1 2 0 3 0 2 0 1 3 1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3 0 0 0 0 1 1
Mitochondria 1 0 0 2 2 14 43 – 62 73 129 90 3 3 0 4 0 2 0 2 7 6 12 6
Nucleus 0 0 2 0 1 0 2 – 1 4 1 1 0 0 0 1 2 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 1 – 0 1 1 0 0 0 0 0 1 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 11 23 19 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 3 0 1 1 1 – 4 1 6 5 9 21 10 0 1 0 0 0 1 0 1 1
Vac/Vac Membrane 0 0 1 0 0 0 1 – 0 1 1 0 1 0 0 3 2 4 2 0 1 3 8 5
Unique Cell Count 47 197 266 181 255 237 366 252 254 306 337 296 217 373 193 172 105 74 207 270 188 330 325
Labelled Cell Count 48 204 285 202 272 256 413 296 303 393 393 348 254 416 224 201 117 74 207 270 188 330 325


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.1 5.6 5.6 5.7 4.6 4.4 – 3.8 3.6 3.4 4.0 5.0 5.0 5.3 6.4 7.1 7.5 4.9 4.9 5.4
Std Deviation (1e-4) 0.5 0.8 0.8 2.0 1.5 1.8 1.3 – 1.3 1.2 0.5 1.0 0.9 0.7 1.2 1.0 1.2 1.6 0.8 1.1 1.6
Intensity Change (Log2) – – – 0.01 0.03 -0.26 -0.35 – -0.55 -0.63 -0.72 -0.47 -0.15 -0.15 -0.08 0.2 0.35 0.42 -0.19 -0.18 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 5.3 4.9 3.0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 5.0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.9 0.6 0 0 – 0 0 0 0 1.2 0.9 2.3 1.2 3.0 0
Cytoplasm 1.4 1.4 -0.8 -2.0 – -6.0 -6.1 -7.8 -6.7 -6.8 -7.7 -4.3 -1.4 -1.4 -4.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 4.3 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 3.2 5.4 3.7 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 1.6 4.3 1.4 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4709 0.9364 0.8649 0.9218 0.7454 1.0118 0.7062 1.239 1.0884 1.0614 0.5501 0.8868 1.0675 1.4217 1.1577 1.6305 1.5725 1.3997
Actin 0.0732 0.0056 0.0232 0.0033 0.0659 0.0152 0.0241 0.0034 0.0302 0.0088 0.0123 0.0164 0.0548 0.0091 0.0139 0.0149 0.0957 0.0076
Bud 0.0013 0.0022 0.0023 0.0114 0.0014 0.0002 0.0021 0.0004 0.0027 0.0055 0.0002 0.0008 0.0014 0.0008 0.0025 0.0052 0.0003 0.0003
Bud Neck 0.0141 0.0016 0.009 0.0106 0.0019 0.0046 0.0056 0.0005 0.002 0.0097 0.0082 0.0067 0.0012 0.0004 0.0004 0.0025 0.0016 0.0018
Bud Periphery 0.0038 0.0048 0.004 0.0159 0.0031 0.0003 0.0036 0.0005 0.0045 0.009 0.0002 0.003 0.0026 0.0007 0.0027 0.0099 0.0002 0.0007
Bud Site 0.0086 0.0257 0.0251 0.0181 0.0029 0.0009 0.0045 0.0046 0.0187 0.0026 0.001 0.0004 0.0063 0.0215 0.0068 0.0567 0.0007 0
Cell Periphery 0.0023 0.0026 0.0037 0.0015 0.0007 0.0005 0.0018 0.0017 0.0019 0.0026 0.0002 0.0005 0.0011 0.0019 0.0015 0.0027 0.0002 0.0002
Cytoplasm 0.3422 0.6014 0.5725 0.4228 0.4135 0.6825 0.5922 0.8336 0.7067 0.6814 0.7422 0.8245 0.5579 0.7266 0.6839 0.5246 0.6698 0.7546
Cytoplasmic Foci 0.0339 0.0174 0.0351 0.1251 0.0046 0.011 0.0264 0.0054 0.0201 0.0393 0.0566 0.007 0.051 0.006 0.014 0.0232 0.0187 0.0036
Eisosomes 0.0011 0.0004 0.0014 0.0001 0.0003 0.0002 0.0004 0.0002 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001 0.0002 0.0004 0.0001 0
Endoplasmic Reticulum 0.0099 0.0041 0.004 0.0032 0.0051 0.0028 0.008 0.0042 0.0038 0.0038 0.0011 0.0017 0.0047 0.0027 0.0042 0.0101 0.0036 0.0027
Endosome 0.03 0.0014 0.0122 0.0132 0.0373 0.008 0.0468 0.0032 0.013 0.0328 0.0059 0.0061 0.0342 0.003 0.0129 0.0773 0.0761 0.0018
Golgi 0.0103 0.0005 0.0044 0.0035 0.0151 0.0189 0.0142 0.0035 0.0075 0.0138 0.0118 0.0038 0.016 0.0037 0.008 0.0284 0.0097 0.0005
Lipid Particles 0.0222 0.0009 0.0067 0.0086 0.0019 0.003 0.0325 0.0015 0.002 0.003 0.0095 0.0022 0.0223 0.0003 0.0017 0.0205 0.0017 0.0001
Mitochondria 0.0204 0.0002 0.0012 0.0014 0.0525 0.0171 0.015 0.001 0.022 0.021 0.0399 0.0067 0.0109 0.0006 0.0138 0.0162 0.0013 0.0001
None 0.3128 0.3266 0.277 0.3369 0.3159 0.231 0.1856 0.1325 0.156 0.1204 0.104 0.1147 0.1984 0.22 0.2251 0.1851 0.1094 0.2076
Nuclear Periphery 0.0324 0.0009 0.0029 0.0012 0.0238 0.0008 0.0092 0.0004 0.0008 0.002 0.0002 0.0007 0.0032 0.0003 0.0007 0.0039 0.0006 0.0003
Nucleolus 0.0053 0.0002 0.0003 0.0027 0.0003 0.0001 0.001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0.0001 0.0001 0.0007 0 0
Nucleus 0.0261 0.0009 0.0015 0.0027 0.0411 0.0004 0.003 0.0006 0.001 0.0013 0.0006 0.0007 0.0008 0.0005 0.0007 0.0022 0.0004 0.0003
Peroxisomes 0.0114 0.0006 0.007 0.0048 0.0007 0.0007 0.0134 0.0015 0.003 0.0116 0.0042 0.0025 0.0205 0.0003 0.004 0.0031 0.0059 0.0001
Punctate Nuclear 0.0303 0.0006 0.0049 0.0093 0.0005 0.0006 0.002 0.0002 0.0011 0.0005 0.001 0.0006 0.0078 0.0003 0.0007 0.0015 0.0003 0.0001
Vacuole 0.0048 0.0013 0.0014 0.0032 0.0078 0.0005 0.0059 0.001 0.0019 0.0219 0.0005 0.0006 0.0026 0.0011 0.0017 0.0079 0.0024 0.0163
Vacuole Periphery 0.0039 0.0001 0.0003 0.0004 0.0038 0.0007 0.0027 0.0001 0.0006 0.0087 0.0003 0.0002 0.0012 0.0001 0.0004 0.0028 0.001 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.0637 11.3226 14.2186 10.7884 7.8952 10.7115 11.5584 13.3734 13.4186 11.0591
Translational Efficiency 0.9535 0.8305 0.6319 0.9041 0.7842 0.6286 0.7039 0.756 0.6979 0.6982

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2030 179 349 1408 1771 1133 2409 176 3801 1312 2758 1584

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 715.69 845.50 994.43 909.38 740.34 704.49 913.90 979.01 727.18 723.73 924.09 917.12
Standard Deviation 77.44 107.58 103.61 103.34 80.01 197.54 101.76 129.44 79.60 193.96 105.45 108.78
Intensity Change Log 2 — 0.240470 0.474535 0.345548 — -0.071609 0.303848 0.403136 — 0.090219 0.390271 0.375117

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004949 0.001249 0.002545 0.014467 0.001352 0.001916 0.000912 0.041630 0.003273 0.001825 0.001118 0.017485
Bud Neck 0.030468 0.025864 0.005481 0.005582 0.020036 0.030201 0.004173 0.003870 0.025607 0.029609 0.004338 0.005392
Bud Site 0.019198 0.024331 0.025167 0.066086 0.010708 0.019842 0.015663 0.103033* 0.015242 0.020455 0.016865 0.070191
Cell Periphery 0.000701 0.000234 0.000497 0.000365 0.000358 0.000196 0.000174 0.001177 0.000541 0.000201 0.000215 0.000455
Cytoplasm 0.568734* 0.375527* 0.440459* 0.609708* 0.554374* 0.433190* 0.701427* 0.366901* 0.562043* 0.425323* 0.668404* 0.582729*
Cytoplasmic Foci 0.185508* 0.317336* 0.047374 0.036748 0.217838* 0.275266* 0.029478 0.068402 0.200571* 0.281006* 0.031742 0.040265
Eisosomes 0.002142 0.000334 0.000124 0.000244 0.000759 0.000469 0.000078 0.000766 0.001498 0.000450 0.000084 0.000302
Endoplasmic Reticulum 0.000974 0.001352 0.008767 0.000910 0.001229 0.000631 0.000803 0.002229 0.001093 0.000729 0.001811 0.001056
Endosome 0.009659 0.035057 0.005293 0.002201 0.012009 0.042100 0.001723 0.003882 0.010754 0.041139 0.002175 0.002388
Golgi 0.006264 0.007815 0.000198 0.000254 0.005141 0.007162 0.000256 0.000251 0.005741 0.007251 0.000249 0.000253
Lipid Particles 0.005049 0.010945 0.003333 0.000825 0.006178 0.009237 0.000665 0.001691 0.005575 0.009470 0.001003 0.000921
Mitochondria 0.004323 0.005796 0.000252 0.000824 0.004905 0.005005 0.000281 0.000445 0.004594 0.005113 0.000278 0.000782
Mitotic Spindle 0.000648 0.002247 0.002265 0.007540 0.001629 0.002019 0.007930 0.009576 0.001105 0.002050 0.007213 0.007766
None 0.005118 0.000320 0.002525 0.001433 0.001779 0.001586 0.001130 0.001601 0.003562 0.001414 0.001306 0.001452
Nuclear Periphery 0.000073 0.000164 0.001064 0.000192 0.000143 0.000243 0.000268 0.000234 0.000105 0.000232 0.000369 0.000196
Nuclear Periphery Foci 0.000481 0.000855 0.007630 0.001487 0.000410 0.000538 0.000990 0.002628 0.000448 0.000581 0.001830 0.001614
Nucleolus 0.000497 0.000429 0.000160 0.000068 0.000556 0.001580 0.000081 0.000083 0.000524 0.001423 0.000091 0.000070
Nucleus 0.073299 0.030448 0.244028* 0.063591 0.077087 0.032316 0.078262 0.034565 0.075064 0.032061 0.099238 0.060366
Peroxisomes 0.009961 0.018970 0.001343 0.002385 0.004476 0.020547 0.001465 0.008880 0.007405 0.020332 0.001449 0.003106
Vacuole 0.071354 0.139347 0.201328* 0.184232 0.078074 0.114142 0.153201 0.347715* 0.074485 0.117580 0.159291 0.202397*
Vacuole Periphery 0.000601 0.001384 0.000166 0.000860 0.000958 0.001814 0.001039 0.000441 0.000767 0.001755 0.000928 0.000813

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.99 1.99 -1.24 -3.75 -2.60 -0.82 1.17 -2.46 -2.38 -2.57 2.20 4.12 -3.06 -4.04 -4.68
Bud Neck 0.81 14.28 17.34 3.56 3.82 -4.25 13.78 13.91 13.13 1.17 -1.70 21.62 21.38 13.09 0.62
Bud Site -0.95 -1.94 -4.92 -1.75 -1.43 -5.12 -0.84 -3.66 -2.80 -3.55 -3.09 1.08 -7.13 -5.00 -7.40
Cell Periphery 3.59 1.26 3.38 -1.02 5.88 5.63 7.51 -1.19 -1.78 -1.96 4.91 5.00 2.91 -3.18 -3.35
Cytoplasm 9.30 13.47 6.23 -6.39 -8.97 12.17 -7.66 11.28 5.16 14.23 16.09 -3.35 8.70 -6.30 10.27
Cytoplasmic Foci -7.63 26.71 37.99 16.82 3.41 -7.56 43.57 25.22 27.68 -3.95 -12.26 56.82 52.55 40.38 -2.49
Eisosomes 5.55 6.18 6.10 2.64 -0.95 3.20 9.16 2.38 0.19 -3.32 5.77 8.03 7.31 4.66 -5.09
Endoplasmic Reticulum -0.86 -10.04 -0.56 0.65 10.39 3.20 1.54 -3.62 -5.55 -4.68 1.68 -4.48 -1.09 -4.04 4.91
Endosome -4.30 6.04 14.79 5.73 9.21 -11.97 16.63 11.45 15.78 -3.15 -13.29 21.02 20.69 17.54 2.00
Golgi -0.65 15.00 15.14 3.81 2.14 -2.07 12.22 12.34 8.21 0.31 -1.70 19.22 19.49 9.03 0.54
Lipid Particles -1.09 2.81 13.16 1.84 9.15 -2.67 14.46 7.79 6.13 -3.88 -3.24 18.50 17.83 6.91 -1.20
Mitochondria -0.29 5.05 4.76 1.23 -0.55 -0.04 4.25 4.29 5.23 0.56 -0.39 6.52 6.14 4.75 -0.61
Mitotic Spindle -1.33 -1.78 -3.51 -1.56 -1.12 -0.41 -3.48 -1.41 -1.29 -0.04 -1.34 -4.40 -3.40 -2.32 0.63
None 7.60 4.61 5.87 -9.32 2.46 0.35 1.73 1.69 1.20 0.64 4.12 6.03 5.78 0.25 -0.47
Nuclear Periphery -3.01 -16.30 -18.95 -5.69 12.16 -2.18 -15.79 -6.25 -3.66 0.03 -3.33 -24.77 -16.97 -3.89 8.83
Nuclear Periphery Foci -2.71 -12.50 -7.43 -3.65 10.41 -1.79 -7.18 -7.76 -7.38 -6.07 -2.32 -13.11 -9.58 -8.20 0.79
Nucleolus 0.67 12.82 22.45* 3.50 6.09 -2.43 2.69 2.73 3.92 0.46 -2.64 5.15 5.43 4.07 3.26
Nucleus 11.22 -13.82 -4.51 -13.19 12.34 16.42 -5.99 6.13 -2.83 9.69 20.70 -13.00 -2.92 -17.14 8.18
Peroxisomes -2.69 7.50 9.55 5.49 0.78 -7.58 6.66 0.51 6.56 -1.75 -6.75 11.15 9.84 10.09 -0.89
Vacuole -5.62 -14.90 -31.02 -10.90 -2.26 -7.79 -27.85 -18.15 -16.29 -11.24 -10.38 -32.95 -34.77 -25.37 -10.84
Vacuole Periphery -1.86 12.16 1.17 2.21 -0.66 -3.11 1.01 3.96 6.82 1.06 -4.68 0.79 2.08 5.09 0.56
Standard name
Human Ortholog
Description Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Yta6

Yta6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yta6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available