Standard name | |
---|---|
Human Ortholog | |
Description | Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity; may have a role in ensuring that soluble TBP is available to bind TATA-less promoters; forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA; coregulates transcription with Spt16p through assembly of preinitiation complex and organization of nucleosomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 9 | 9 | 8 | 2 | 7 | 0 | 1 | 3 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 3 | 4 | 5 | 13 | 3 | 15 | 14 | 4 | 11 | 1 | 8 | 5 | 18 | 16 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 7 | 14 | 9 | 19 | 50 | 67 | 118 | 69 | 93 | 39 | 80 | 1 | 0 | 5 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 2 |
Nucleus | 236 | 81 | 290 | 133 | 235 | 182 | 283 | 315 | 116 | 260 | 117 | 136 | 99 | 163 | 168 | 15 | 34 | 10 | 235 | 89 | 303 | 127 | 194 | 178 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 1 | 2 | 9 | 2 | 11 | 12 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 16 | 16 | 28 | 45 | 58 | 74 | 118 | 55 | 112 | 47 | 61 | 9 | 6 | 31 | 0 | 11 | 5 | 4 | 4 | 5 | 8 | 24 | 63 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 3 | 2 | 1 | 1 | 3 | 3 | 5 | 11 | 42 | 59 |
Unique Cell Count | 244 | 95 | 309 | 147 | 279 | 236 | 351 | 431 | 175 | 353 | 153 | 207 | 105 | 185 | 204 | 19 | 42 | 16 | 251 | 101 | 320 | 150 | 270 | 315 |
Labelled Cell Count | 247 | 108 | 329 | 175 | 314 | 297 | 451 | 578 | 264 | 501 | 214 | 300 | 114 | 192 | 236 | 20 | 48 | 18 | 251 | 101 | 320 | 150 | 270 | 315 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.3 | 11.5 | 8.5 | 8.0 | 7.6 | 6.6 | 6.2 | 5.9 | 5.6 | 5.5 | 5.2 | 5.3 | 12.8 | 13.3 | 10.4 | 18.2 | 18.5 | 20.2 | 13.5 | 15.2 | 16.0 |
Std Deviation (1e-4) | 4.3 | 4.0 | 2.2 | 2.8 | 2.1 | 1.6 | 1.8 | 1.5 | 1.5 | 1.4 | 1.3 | 1.6 | 3.5 | 5.8 | 4.7 | 8.1 | 4.7 | 7.1 | 3.9 | 4.9 | 4.8 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.15 | -0.36 | -0.46 | -0.51 | -0.6 | -0.61 | -0.7 | -0.68 | 0.6 | 0.65 | 0.3 | 1.11 | 1.13 | 1.25 | 0.67 | 0.84 | 0.92 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.4 | 0 | 2.3 | 1.7 | 0 | 1.6 | 0 | 0 | 0 | 4.4 | 3.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.7 | 1.2 | 6.0 | 5.7 | 8.0 | 9.8 | 7.6 | 6.7 | 9.8 | 0 | -2.9 | -1.2 | 0 | 0 | 0 |
Nucleus | -1.3 | -3.8 | -5.7 | -5.0 | -7.2 | -7.9 | -6.9 | -5.4 | -8.3 | 0.2 | -2.2 | -4.1 | -2.5 | -2.9 | -4.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4.7 | 4.3 | 6.6 | 5.9 | 7.7 | 7.8 | 8.6 | 7.5 | 7.6 | 1.3 | -1.0 | 3.8 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.9844 | 11.825 | 12.1685 | 11.6323 | 11.9142 | 11.0867 | 13.0602 | 15.3026 | 14.2179 | 12.8831 | 13.1053 | 14.5035 | 16.2451 | 15.1082 | 14.9406 | 11.5808 | 12.4325 | 13.525 |
Actin | 0.0057 | 0.0011 | 0 | 0.002 | 0.0351 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0085 | 0 | 0.0002 | 0.0432 | 0 | 0.0094 | 0.0017 | 0 |
Bud | 0.0003 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0017 | 0 | 0.0002 | 0.0004 | 0 |
Bud Neck | 0.0018 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.0001 |
Bud Periphery | 0.0006 | 0 | 0 | 0 | 0.0007 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0016 | 0 | 0.0002 | 0.0012 | 0 |
Bud Site | 0.0028 | 0.0001 | 0 | 0.0001 | 0.0013 | 0 | 0.0003 | 0.0016 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0109 | 0.0001 | 0.0007 | 0.0006 | 0 |
Cell Periphery | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 |
Cytoplasm | 0.0097 | 0.0005 | 0 | 0.0036 | 0.0017 | 0.0018 | 0.0009 | 0.0013 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0787 | 0.0015 | 0.0009 | 0.0003 | 0.0005 |
Cytoplasmic Foci | 0.009 | 0.0003 | 0 | 0.0004 | 0.0108 | 0 | 0.0012 | 0.0004 | 0.0008 | 0 | 0.0025 | 0 | 0 | 0.0074 | 0.0005 | 0.014 | 0.0003 | 0.0001 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0026 | 0.0004 | 0 | 0.0006 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0011 | 0.0011 | 0.0016 | 0.0002 | 0 |
Endosome | 0.0099 | 0.0014 | 0 | 0.0015 | 0.0223 | 0.0001 | 0.0003 | 0.0008 | 0.0002 | 0 | 0.0014 | 0 | 0.0001 | 0.0045 | 0.025 | 0.0521 | 0.0009 | 0 |
Golgi | 0.0039 | 0.0002 | 0 | 0.0001 | 0.0073 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0014 | 0 | 0 | 0.0047 | 0.0007 | 0.0315 | 0.0004 | 0 |
Lipid Particles | 0.0102 | 0.0006 | 0 | 0.0001 | 0.0122 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0021 | 0 | 0 | 0.0018 | 0.0002 | 0.0328 | 0.0015 | 0 |
Mitochondria | 0.0108 | 0.0006 | 0.0004 | 0.0008 | 0.0045 | 0.0006 | 0.0065 | 0.0005 | 0.0008 | 0.0003 | 0.0015 | 0.0005 | 0.0013 | 0.0045 | 0.0004 | 0.0098 | 0.0666 | 0.0002 |
None | 0.0135 | 0.0013 | 0 | 0.0012 | 0.0039 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0007 | 0.0007 | 0.0009 | 0.0004 | 0.0027 |
Nuclear Periphery | 0.0136 | 0.0097 | 0.0004 | 0.0099 | 0.0088 | 0.0174 | 0.0047 | 0.0023 | 0.0021 | 0.0002 | 0.0083 | 0.0001 | 0.0105 | 0.0056 | 0.0037 | 0.0032 | 0.0057 | 0.0004 |
Nucleolus | 0.0204 | 0.0162 | 0.018 | 0.0074 | 0.0427 | 0.0314 | 0.0387 | 0.0097 | 0.0145 | 0.016 | 0.0741 | 0.0225 | 0.0068 | 0.0117 | 0.0068 | 0.006 | 0.0832 | 0.0339 |
Nucleus | 0.8707 | 0.9588 | 0.981 | 0.9645 | 0.8154 | 0.9453 | 0.8834 | 0.9794 | 0.9767 | 0.9828 | 0.8701 | 0.9766 | 0.98 | 0.7378 | 0.9505 | 0.8264 | 0.8293 | 0.9473 |
Peroxisomes | 0.0022 | 0.0001 | 0 | 0.0001 | 0.0125 | 0 | 0.0002 | 0 | 0 | 0 | 0.0025 | 0 | 0 | 0.0039 | 0 | 0.0062 | 0.0004 | 0 |
Punctate Nuclear | 0.0064 | 0.008 | 0.0001 | 0.0072 | 0.0167 | 0.002 | 0.0592 | 0.0017 | 0.0034 | 0 | 0.0259 | 0 | 0.0003 | 0.0778 | 0.0037 | 0.0019 | 0.001 | 0.0148 |
Vacuole | 0.0028 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0007 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0008 | 0.0034 | 0.0007 | 0.0014 | 0 |
Vacuole Periphery | 0.0023 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0.0013 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0007 | 0.0017 | 0.0011 | 0.0037 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.6639 | 28.1621 | 22.726 | 28.6479 | 32.4211 | 24.008 | 31.1441 | 22.4616 | 24.7662 | 45.5147 |
Translational Efficiency | 0.4371 | 0.3622 | 0.4043 | 0.3337 | 0.4002 | 0.3852 | 0.316 | 0.3164 | 0.3285 | 0.2939 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
413 | 199 | 2512 | 1560 | 2152 | 2346 | 2171 | 960 | 2565 | 2545 | 4683 | 2520 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 957.88 | 1393.34 | 1793.95 | 1572.36 | 1118.28 | 1359.76 | 1771.91 | 1566.02 | 1092.45 | 1362.39 | 1783.73 | 1569.94 |
Standard Deviation | 190.35 | 300.52 | 338.80 | 327.75 | 258.39 | 267.57 | 319.41 | 344.02 | 255.58 | 270.44 | 330.14 | 334.05 |
Intensity Change Log 2 | — | 0.540631 | 0.905223 | 0.715015 | — | 0.282071 | 0.664024 | 0.485821 | — | 0.407139 | 0.780332 | 0.596102 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000507 | 0.000315 | 0.000311 | 0.000593 | 0.000394 | 0.000435 | 0.000412 | 0.000603 | 0.000413 | 0.000425 | 0.000358 | 0.000597 |
Bud Neck | 0.004064 | 0.005653 | 0.008345 | 0.016919 | 0.004789 | 0.005830 | 0.008139 | 0.011916 | 0.004672 | 0.005816 | 0.008250 | 0.015013 |
Bud Site | 0.000514 | 0.000461 | 0.000388 | 0.002343 | 0.000539 | 0.000734 | 0.000893 | 0.003139 | 0.000535 | 0.000713 | 0.000622 | 0.002646 |
Cell Periphery | 0.000154 | 0.000040 | 0.000062 | 0.000160 | 0.000347 | 0.000297 | 0.000197 | 0.000227 | 0.000316 | 0.000277 | 0.000124 | 0.000186 |
Cytoplasm | 0.001087 | 0.002434 | 0.000037 | 0.000743 | 0.000129 | 0.000067 | 0.000091 | 0.001408 | 0.000283 | 0.000252 | 0.000062 | 0.000996 |
Cytoplasmic Foci | 0.000150 | 0.000132 | 0.000013 | 0.001733 | 0.000010 | 0.000030 | 0.000147 | 0.001468 | 0.000033 | 0.000038 | 0.000075 | 0.001632 |
Eisosomes | 0.000039 | 0.000045 | 0.000098 | 0.000056 | 0.000145 | 0.000168 | 0.000147 | 0.000073 | 0.000128 | 0.000158 | 0.000121 | 0.000063 |
Endoplasmic Reticulum | 0.002023 | 0.000649 | 0.000519 | 0.000897 | 0.002498 | 0.002380 | 0.001069 | 0.000826 | 0.002421 | 0.002245 | 0.000774 | 0.000870 |
Endosome | 0.000162 | 0.000021 | 0.000006 | 0.002494 | 0.000047 | 0.000138 | 0.000095 | 0.000997 | 0.000066 | 0.000129 | 0.000047 | 0.001924 |
Golgi | 0.000056 | 0.000040 | 0.000010 | 0.003106 | 0.000016 | 0.000027 | 0.000026 | 0.001582 | 0.000023 | 0.000028 | 0.000017 | 0.002525 |
Lipid Particles | 0.000137 | 0.000008 | 0.000001 | 0.000244 | 0.000003 | 0.000060 | 0.000019 | 0.000130 | 0.000025 | 0.000056 | 0.000009 | 0.000201 |
Mitochondria | 0.000677 | 0.000539 | 0.000770 | 0.002114 | 0.000626 | 0.000701 | 0.000950 | 0.001726 | 0.000635 | 0.000688 | 0.000853 | 0.001966 |
Mitotic Spindle | 0.000369 | 0.001343 | 0.000017 | 0.005748 | 0.000277 | 0.001090 | 0.000764 | 0.004364 | 0.000292 | 0.001110 | 0.000363 | 0.005221 |
None | 0.002055 | 0.001067 | 0.000287 | 0.002239 | 0.001802 | 0.000495 | 0.000293 | 0.001610 | 0.001843 | 0.000540 | 0.000290 | 0.001999 |
Nuclear Periphery | 0.000099 | 0.000051 | 0.000036 | 0.000613 | 0.000065 | 0.000143 | 0.000071 | 0.000254 | 0.000071 | 0.000136 | 0.000052 | 0.000476 |
Nuclear Periphery Foci | 0.000578 | 0.000088 | 0.000001 | 0.000527 | 0.000028 | 0.000318 | 0.000014 | 0.000226 | 0.000117 | 0.000300 | 0.000007 | 0.000412 |
Nucleolus | 0.020035 | 0.008047 | 0.005493 | 0.011565 | 0.009343 | 0.007938 | 0.004972 | 0.008525 | 0.011064 | 0.007946 | 0.005251 | 0.010407 |
Nucleus | 0.965984* | 0.978338* | 0.983102* | 0.943932* | 0.977646* | 0.977621* | 0.980131* | 0.958351* | 0.975768* | 0.977677* | 0.981725* | 0.949425* |
Peroxisomes | 0.000597 | 0.000510 | 0.000235 | 0.000369 | 0.000241 | 0.000287 | 0.000352 | 0.000547 | 0.000298 | 0.000304 | 0.000289 | 0.000437 |
Vacuole | 0.000556 | 0.000163 | 0.000164 | 0.002190 | 0.000797 | 0.000939 | 0.000775 | 0.001518 | 0.000758 | 0.000878 | 0.000447 | 0.001934 |
Vacuole Periphery | 0.000155 | 0.000057 | 0.000104 | 0.001415 | 0.000257 | 0.000304 | 0.000442 | 0.000511 | 0.000241 | 0.000284 | 0.000261 | 0.001071 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.83 | 3.58 | -2.75 | -7.47 | -13.48 | -0.81 | -0.94 | -5.53 | -3.64 | -4.65 | -0.31 | 2.05 | -9.22 | -4.80 | -10.76 |
Bud Neck | -0.76 | -10.22 | -12.46 | -4.72 | -8.81 | -2.15 | -6.60 | -7.50 | -6.21 | -3.74 | -2.42 | -10.97 | -14.20 | -11.61 | -9.35 |
Bud Site | -0.15 | 0.87 | -4.94 | -4.04 | -5.74 | -0.85 | -1.65 | -2.77 | -2.55 | -2.17 | -0.90 | -0.90 | -4.83 | -4.40 | -4.56 |
Cell Periphery | 1.09 | 0.95 | 0.03 | -4.45 | -4.19 | 0.53 | 1.49 | 1.22 | 0.69 | -0.31 | 0.46 | 2.94 | 2.14 | 1.37 | -1.23 |
Cytoplasm | -0.61 | 2.72 | 0.94 | 0.79 | -5.56 | 1.49 | -0.00 | -2.51 | -2.61 | -2.51 | 0.07 | 2.98 | -3.25 | -2.57 | -4.44 |
Cytoplasmic Foci | -0.63 | 1.56 | -2.77 | -1.99 | -3.21 | -1.54 | -1.57 | -2.45 | -2.41 | -1.69 | -0.69 | -1.33 | -3.96 | -3.89 | -3.40 |
Eisosomes | -1.01 | -10.94 | -5.06 | -2.07 | 7.53 | -0.86 | -0.04 | 3.04 | 6.32 | 6.35 | -1.31 | 0.35 | 3.33 | 7.10 | 9.55 |
Endoplasmic Reticulum | 7.19 | 8.26 | 5.58 | -4.02 | -8.24 | 0.53 | 5.22 | 5.96 | 5.97 | -0.24 | 0.98 | 9.55 | 8.86 | 8.46 | -2.18 |
Endosome | 1.63 | 1.90 | -2.73 | -3.08 | -3.13 | -1.93 | -0.96 | -2.32 | -2.04 | -2.17 | -1.21 | 0.88 | -3.60 | -3.44 | -3.67 |
Golgi | -0.02 | 3.06 | -2.95 | -2.95 | -3.02 | -1.58 | -1.23 | -1.83 | -1.82 | -1.82 | -0.89 | 0.85 | -3.51 | -3.50 | -3.52 |
Lipid Particles | 1.44 | 1.61 | -0.33 | -1.80 | -1.96 | -1.41 | -1.82 | -2.82 | -1.38 | -2.42 | -0.76 | 1.00 | -2.31 | -1.62 | -2.61 |
Mitochondria | 1.43 | -1.72 | -9.65 | -10.65 | -9.44 | -1.10 | -6.57 | -10.06 | -8.64 | -7.11 | -0.90 | -6.09 | -13.72 | -11.93 | -11.50 |
Mitotic Spindle | -0.82 | 1.23 | -3.59 | -2.17 | -3.96 | -1.61 | -1.00 | -2.69 | -2.09 | -2.27 | -1.75 | -0.30 | -4.53 | -3.53 | -4.45 |
None | 1.01 | 2.97 | -0.20 | -1.34 | -4.07 | 3.96 | 4.79 | 0.26 | -2.97 | -3.59 | 4.32 | 5.55 | -0.42 | -4.24 | -5.23 |
Nuclear Periphery | 1.45 | 2.90 | -4.06 | -4.44 | -4.67 | -2.14 | -1.27 | -5.35 | -2.80 | -4.83 | -1.93 | 1.44 | -5.43 | -4.20 | -5.59 |
Nuclear Periphery Foci | 1.07 | 1.62 | 0.74 | -0.68 | -2.33 | -1.52 | -0.06 | -2.12 | 0.11 | -2.11 | -0.80 | 1.71 | -1.48 | -0.30 | -2.95 |
Nucleolus | 2.06 | 6.28 | 2.36 | -0.86 | -5.93 | 1.43 | 4.00 | -0.46 | -1.28 | -2.81 | 3.02 | 6.44 | -1.38 | -3.85 | -6.51 |
Nucleus | -1.07 | -4.95 | 4.91 | 4.00 | 10.82 | 0.18 | -0.25 | 5.27 | 5.07 | 5.20 | -0.76 | -3.31 | 9.84 | 9.73 | 11.57 |
Peroxisomes | 0.17 | 3.07 | 1.24 | 0.50 | -3.28 | -1.02 | -1.72 | -3.51 | -2.91 | -1.36 | -0.25 | -0.79 | -4.08 | -3.41 | -2.63 |
Vacuole | 1.71 | 1.85 | -2.48 | -3.87 | -3.96 | -0.77 | -0.59 | -2.35 | -2.08 | -1.87 | -0.60 | 1.37 | -3.50 | -3.08 | -3.94 |
Vacuole Periphery | 3.18 | 1.99 | -2.67 | -2.93 | -2.82 | -0.46 | -0.58 | -1.56 | -1.12 | -0.16 | -0.48 | -0.10 | -2.72 | -2.46 | -2.38 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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