Standard name | |
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Human Ortholog | |
Description | Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity; may have a role in ensuring that soluble TBP is available to bind TATA-less promoters; forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA; coregulates transcription with Spt16p through assembly of preinitiation complex and organization of nucleosomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 9 | 9 | 8 | 2 | 7 | 0 | 1 | 3 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 3 | 4 | 5 | 13 | 3 | 15 | 14 | 4 | 11 | 1 | 8 | 5 | 18 | 16 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 7 | 14 | 9 | 19 | 50 | 67 | 118 | 69 | 93 | 39 | 80 | 1 | 0 | 5 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 2 |
Nucleus | 236 | 81 | 290 | 133 | 235 | 182 | 283 | 315 | 116 | 260 | 117 | 136 | 99 | 163 | 168 | 15 | 34 | 10 | 235 | 89 | 303 | 127 | 194 | 178 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 1 | 2 | 9 | 2 | 11 | 12 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 16 | 16 | 28 | 45 | 58 | 74 | 118 | 55 | 112 | 47 | 61 | 9 | 6 | 31 | 0 | 11 | 5 | 4 | 4 | 5 | 8 | 24 | 63 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 3 | 2 | 1 | 1 | 3 | 3 | 5 | 11 | 42 | 59 |
Unique Cell Count | 244 | 95 | 309 | 147 | 279 | 236 | 351 | 431 | 175 | 353 | 153 | 207 | 105 | 185 | 204 | 19 | 42 | 16 | 251 | 101 | 320 | 150 | 270 | 315 |
Labelled Cell Count | 247 | 108 | 329 | 175 | 314 | 297 | 451 | 578 | 264 | 501 | 214 | 300 | 114 | 192 | 236 | 20 | 48 | 18 | 251 | 101 | 320 | 150 | 270 | 315 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.3 | 11.5 | 8.5 | 8.0 | 7.6 | 6.6 | 6.2 | 5.9 | 5.6 | 5.5 | 5.2 | 5.3 | 12.8 | 13.3 | 10.4 | 18.2 | 18.5 | 20.2 | 13.5 | 15.2 | 16.0 |
Std Deviation (1e-4) | 4.3 | 4.0 | 2.2 | 2.8 | 2.1 | 1.6 | 1.8 | 1.5 | 1.5 | 1.4 | 1.3 | 1.6 | 3.5 | 5.8 | 4.7 | 8.1 | 4.7 | 7.1 | 3.9 | 4.9 | 4.8 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.15 | -0.36 | -0.46 | -0.51 | -0.6 | -0.61 | -0.7 | -0.68 | 0.6 | 0.65 | 0.3 | 1.11 | 1.13 | 1.25 | 0.67 | 0.84 | 0.92 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.4 | 0 | 2.3 | 1.7 | 0 | 1.6 | 0 | 0 | 0 | 4.4 | 3.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.7 | 1.2 | 6.0 | 5.7 | 8.0 | 9.8 | 7.6 | 6.7 | 9.8 | 0 | -2.9 | -1.2 | 0 | 0 | 0 |
Nucleus | -1.3 | -3.8 | -5.7 | -5.0 | -7.2 | -7.9 | -6.9 | -5.4 | -8.3 | 0.2 | -2.2 | -4.1 | -2.5 | -2.9 | -4.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4.7 | 4.3 | 6.6 | 5.9 | 7.7 | 7.8 | 8.6 | 7.5 | 7.6 | 1.3 | -1.0 | 3.8 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.9844 | 11.825 | 12.1685 | 11.6323 | 11.9142 | 11.0867 | 13.0602 | 15.3026 | 14.2179 | 12.8831 | 13.1053 | 14.5035 | 16.2451 | 15.1082 | 14.9406 | 11.5808 | 12.4325 | 13.525 |
Actin | 0.0057 | 0.0011 | 0 | 0.002 | 0.0351 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0085 | 0 | 0.0002 | 0.0432 | 0 | 0.0094 | 0.0017 | 0 |
Bud | 0.0003 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0017 | 0 | 0.0002 | 0.0004 | 0 |
Bud Neck | 0.0018 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.0001 |
Bud Periphery | 0.0006 | 0 | 0 | 0 | 0.0007 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0016 | 0 | 0.0002 | 0.0012 | 0 |
Bud Site | 0.0028 | 0.0001 | 0 | 0.0001 | 0.0013 | 0 | 0.0003 | 0.0016 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0109 | 0.0001 | 0.0007 | 0.0006 | 0 |
Cell Periphery | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 |
Cytoplasm | 0.0097 | 0.0005 | 0 | 0.0036 | 0.0017 | 0.0018 | 0.0009 | 0.0013 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0787 | 0.0015 | 0.0009 | 0.0003 | 0.0005 |
Cytoplasmic Foci | 0.009 | 0.0003 | 0 | 0.0004 | 0.0108 | 0 | 0.0012 | 0.0004 | 0.0008 | 0 | 0.0025 | 0 | 0 | 0.0074 | 0.0005 | 0.014 | 0.0003 | 0.0001 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0026 | 0.0004 | 0 | 0.0006 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0011 | 0.0011 | 0.0016 | 0.0002 | 0 |
Endosome | 0.0099 | 0.0014 | 0 | 0.0015 | 0.0223 | 0.0001 | 0.0003 | 0.0008 | 0.0002 | 0 | 0.0014 | 0 | 0.0001 | 0.0045 | 0.025 | 0.0521 | 0.0009 | 0 |
Golgi | 0.0039 | 0.0002 | 0 | 0.0001 | 0.0073 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0014 | 0 | 0 | 0.0047 | 0.0007 | 0.0315 | 0.0004 | 0 |
Lipid Particles | 0.0102 | 0.0006 | 0 | 0.0001 | 0.0122 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0021 | 0 | 0 | 0.0018 | 0.0002 | 0.0328 | 0.0015 | 0 |
Mitochondria | 0.0108 | 0.0006 | 0.0004 | 0.0008 | 0.0045 | 0.0006 | 0.0065 | 0.0005 | 0.0008 | 0.0003 | 0.0015 | 0.0005 | 0.0013 | 0.0045 | 0.0004 | 0.0098 | 0.0666 | 0.0002 |
None | 0.0135 | 0.0013 | 0 | 0.0012 | 0.0039 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0007 | 0.0007 | 0.0009 | 0.0004 | 0.0027 |
Nuclear Periphery | 0.0136 | 0.0097 | 0.0004 | 0.0099 | 0.0088 | 0.0174 | 0.0047 | 0.0023 | 0.0021 | 0.0002 | 0.0083 | 0.0001 | 0.0105 | 0.0056 | 0.0037 | 0.0032 | 0.0057 | 0.0004 |
Nucleolus | 0.0204 | 0.0162 | 0.018 | 0.0074 | 0.0427 | 0.0314 | 0.0387 | 0.0097 | 0.0145 | 0.016 | 0.0741 | 0.0225 | 0.0068 | 0.0117 | 0.0068 | 0.006 | 0.0832 | 0.0339 |
Nucleus | 0.8707 | 0.9588 | 0.981 | 0.9645 | 0.8154 | 0.9453 | 0.8834 | 0.9794 | 0.9767 | 0.9828 | 0.8701 | 0.9766 | 0.98 | 0.7378 | 0.9505 | 0.8264 | 0.8293 | 0.9473 |
Peroxisomes | 0.0022 | 0.0001 | 0 | 0.0001 | 0.0125 | 0 | 0.0002 | 0 | 0 | 0 | 0.0025 | 0 | 0 | 0.0039 | 0 | 0.0062 | 0.0004 | 0 |
Punctate Nuclear | 0.0064 | 0.008 | 0.0001 | 0.0072 | 0.0167 | 0.002 | 0.0592 | 0.0017 | 0.0034 | 0 | 0.0259 | 0 | 0.0003 | 0.0778 | 0.0037 | 0.0019 | 0.001 | 0.0148 |
Vacuole | 0.0028 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0007 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0008 | 0.0034 | 0.0007 | 0.0014 | 0 |
Vacuole Periphery | 0.0023 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0.0013 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0007 | 0.0017 | 0.0011 | 0.0037 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.6639 | 28.1621 | 22.726 | 28.6479 | 32.4211 | 24.008 | 31.1441 | 22.4616 | 24.7662 | 45.5147 |
Translational Efficiency | 0.4371 | 0.3622 | 0.4043 | 0.3337 | 0.4002 | 0.3852 | 0.316 | 0.3164 | 0.3285 | 0.2939 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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