Standard name
Human Ortholog
Description Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.22 0.31 0.19 0.15 0.11 0.2 0.26 0.2 – 0.16 0.25 0.12 0.08 0.07 0.18 0.13 0.11 0 0 0 0.05 0 0.05
Bud 0 0 0.06 0 0 0 0.06 0.08 0.09 – 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.1 0.17 0 0.06 0.06 0.05 0.09 0.08 – 0.09 0.1 0.07 0.08 0.18 0.31 0.32 0.31 0.05 0 0 0 0 0
Endoplasmic Reticulum 0 0.16 0.17 0 0 0 0.06 0 0.08 – 0.06 0.1 0.1 0.09 0.12 0.06 0 0.06 0 0 0 0 0 0
Endosome 0.75 0.51 0.36 0.63 0.61 0.69 0.56 0.49 0.41 – 0.42 0.37 0.65 0.63 0.59 0.2 0.26 0.24 0.67 0.76 0.73 0.78 0.81 0.78
Golgi 0.07 0.13 0 0.09 0.07 0 0.06 0.08 0 – 0 0.06 0.09 0.15 0.1 0.16 0.21 0.15 0.07 0.06 0 0 0.07 0.1
Mitochondria 0.15 0.06 0 0.09 0 0.1 0.07 0 0.27 – 0.35 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.07 0.07 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.16 0.22 0.12 0.19 0.23 0.07 – 0.11 0.16 0 0 0 0.11 0.07 0.05 0.07 0 0 0 0 0
Vac/Vac Membrane 0.07 0.14 0.1 0.06 0 0.06 0 0.06 0 – 0 0.06 0.11 0.06 0 0.18 0.2 0.26 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 30 83 39 46 35 100 105 68 – 63 84 42 34 29 50 21 15 1 5 6 8 15 20
Bud 2 2 15 5 9 12 32 32 31 – 28 32 8 9 14 4 3 2 0 1 1 0 0 1
Bud Neck 0 0 0 0 0 0 2 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 2 – 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 2 0 1 – 0 1 0 0 0 0 1 2 0 0 0 0 0 0
Cytoplasm 4 14 47 7 20 20 26 37 28 – 36 32 25 34 79 86 53 42 4 5 15 2 0 0
Endoplasmic Reticulum 1 22 46 8 8 8 31 17 25 – 23 34 34 38 52 16 4 8 0 0 6 4 2 4
Endosome 53 71 97 127 192 214 277 197 137 – 167 123 221 277 259 55 43 32 71 123 258 129 356 359
Golgi 5 18 10 19 21 12 31 34 8 – 17 20 31 67 43 45 35 20 7 9 12 6 33 44
Mitochondria 11 9 1 19 9 31 36 12 91 – 137 50 7 2 3 8 6 0 2 0 13 1 2 0
Nucleus 0 0 0 0 0 0 0 0 3 – 0 0 0 0 0 2 1 1 0 0 0 0 0 0
Nuclear Periphery 1 2 0 0 3 2 1 3 3 – 4 0 2 1 0 0 0 0 1 0 0 0 1 1
Nucleolus 5 4 1 8 23 21 16 8 11 – 12 8 5 6 10 2 1 1 0 0 0 0 0 1
Peroxisomes 0 0 0 3 6 4 14 7 3 – 7 3 12 7 5 0 0 1 2 2 3 1 10 13
SpindlePole 2 1 28 32 71 38 94 95 23 – 43 54 9 18 11 30 12 7 6 5 7 1 10 4
Vac/Vac Membrane 5 20 28 12 14 17 19 25 13 – 17 21 36 25 18 51 33 35 1 4 23 5 3 3
Unique Cell Count 71 139 269 202 316 309 493 406 333 397 333 341 437 442 280 167 134 107 162 356 166 441 459
Labelled Cell Count 94 193 356 279 422 414 682 572 447 556 462 432 518 523 349 213 166 107 162 356 166 441 459


Cytoplasm, Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 11.1 9.2 8.7 9.8 8.2 8.3 8.7 7.3 – 7.4 7.8 10.8 11.4 11.5 15.6 15.8 15.6 9.2 9.9 10.2
Std Deviation (1e-4) 1.5 1.7 1.3 1.2 1.4 1.2 1.3 1.4 1.4 – 1.4 1.4 1.5 1.5 1.7 3.8 3.2 2.9 1.2 1.6 1.8
Intensity Change (Log2) – – – -0.08 0.09 -0.16 -0.14 -0.08 -0.33 – -0.32 -0.24 0.23 0.31 0.33 0.77 0.78 0.77 0.0 0.1 0.15

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.8 -4.7 -5.8 -3.3 -1.4 -2.9 – -4.6 -1.5 -5.6 -8.0 -8.6 -3.6 -4.4 -4.3
Bud -1.7 -1.7 -1.0 0.5 1.2 1.7 – 0.8 1.8 -2.1 -2.5 -1.6 -2.7 -1.9 -1.9
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -4.7 -4.2 -4.1 -5.5 -3.2 -3.3 – -3.2 -2.8 -3.9 -3.9 0.1 3.6 3.4 3.2
Endoplasmic Reticulum -4.4 -6.1 -6.0 -4.7 -5.6 -3.6 – -4.7 -2.5 -2.6 -3.3 -2.0 -4.2 -4.7 -3.1
Endosome 5.8 6.0 8.0 5.3 3.2 1.3 – 1.6 0.2 7.1 7.1 5.8 -4.3 -2.2 -2.5
Golgi 2.5 1.6 0.1 1.5 2.4 -0.9 – 0.4 1.3 2.6 4.8 3.0 4.8 5.8 4.0
Mitochondria 4.8 0 5.1 4.3 2.4 9.1 – 10.7 6.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 4.2 4.0 2.6 0 2.6 – 2.4 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 3.1 0 0 0 0 0
SpindlePole 1.8 3.9 0.7 3.1 4.3 -1.5 – 0.2 2.1 -4.0 -3.3 -4.5 0.1 -1.1 -1.7
Vacuole -1.7 -2.8 -2.2 -3.6 -2.0 -3.1 – -3.1 -1.8 0.1 -2.3 -3.3 2.6 2.7 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.0439 8.9433 8.1927 7.1829 5.8977 8.4551 5.0178 7.5302 6.7829 5.1567 5.6819 6.4394 5.1888 7.6345 7.1246 6.3037 5.1264 7.0305
Actin 0.0026 0.003 0.0488 0.0416 0.1706 0.0006 0.0242 0.0032 0.0046 0.0006 0.0004 0.004 0.0618 0.0032 0.0083 0.044 0.1224 0.0003
Bud 0.0001 0.0001 0.0005 0.0001 0.0003 0.0001 0.0003 0.0001 0.0004 0.0005 0 0.0002 0.0008 0.0005 0.0004 0.0006 0.0015 0.0001
Bud Neck 0.0003 0.0002 0.0006 0.0012 0.0004 0.0005 0.0027 0.0002 0.0002 0.0004 0.0001 0.0005 0.0015 0.0003 0.0004 0.0002 0.0006 0.0003
Bud Periphery 0.0001 0 0.0003 0.0001 0.0005 0.0001 0.0003 0 0.0001 0.0008 0 0.0001 0.0009 0.0002 0.0004 0.0007 0.0023 0
Bud Site 0.0005 0.0005 0.0014 0.0005 0.0006 0.0002 0.0037 0.0003 0.0007 0.0007 0.0001 0.0002 0.0033 0.002 0.0013 0.0013 0.0015 0.0001
Cell Periphery 0.0001 0 0.0002 0.0001 0.0001 0.0001 0.0002 0 0 0.0002 0 0 0.0003 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0242 0.0456 0.0526 0.0365 0.0092 0.0404 0.0362 0.0349 0.0363 0.0432 0.0292 0.0199 0.0289 0.0713 0.0627 0.0366 0.0076 0.0108
Cytoplasmic Foci 0.2692 0.3723 0.2446 0.2679 0.2291 0.2807 0.2338 0.3495 0.3066 0.274 0.2815 0.2934 0.1664 0.2721 0.2502 0.1794 0.158 0.2881
Eisosomes 0 0 0.0011 0.0004 0.0012 0 0.0001 0 0 0 0 0 0.0006 0 0.0001 0.0007 0.0004 0
Endoplasmic Reticulum 0.0159 0.0013 0.0033 0.0027 0.0015 0.0023 0.0029 0.0006 0.0012 0.0008 0.0005 0.0007 0.0085 0.0012 0.0011 0.0023 0.0039 0.0007
Endosome 0.4672 0.4588 0.4836 0.5496 0.4618 0.4347 0.5079 0.5122 0.5362 0.5066 0.5838 0.5053 0.4933 0.4956 0.5094 0.5428 0.4604 0.5267
Golgi 0.0785 0.0808 0.1071 0.0755 0.0434 0.1616 0.1064 0.0723 0.0814 0.053 0.0928 0.1466 0.1024 0.0674 0.0904 0.0495 0.1208 0.1168
Lipid Particles 0.068 0.0157 0.0171 0.0052 0.0268 0.0497 0.0171 0.0075 0.004 0.0105 0.0013 0.0077 0.0208 0.0105 0.0145 0.0127 0.0193 0.017
Mitochondria 0.0017 0.0006 0.0098 0.0009 0.0062 0.0038 0.0087 0.0004 0.0098 0.0753 0.0002 0.0041 0.0218 0.0006 0.006 0.0325 0.0429 0.0005
None 0.001 0.0002 0.0005 0.0009 0.0026 0.0001 0.0085 0.0001 0.0001 0.0002 0.0001 0.0001 0.0027 0.0022 0.0003 0.0011 0.0015 0.0001
Nuclear Periphery 0.0146 0.0002 0.0005 0.0012 0.0026 0.0002 0.0101 0.0002 0.0006 0.002 0.0001 0.0002 0.0065 0.0005 0.0006 0.0029 0.0297 0.0004
Nucleolus 0.0001 0 0.0001 0.0001 0.0002 0 0.0004 0 0 0.0005 0 0 0.0025 0.0037 0.0004 0.0003 0.0003 0.0002
Nucleus 0.0009 0.0001 0.0001 0.0003 0.0002 0.0001 0.0025 0.0001 0.0001 0.0003 0.0001 0.0001 0.0548 0.0476 0.0282 0.067 0.0045 0.0249
Peroxisomes 0.0405 0.013 0.0127 0.0062 0.0318 0.0209 0.0175 0.0131 0.0105 0.0078 0.006 0.0116 0.0065 0.0104 0.0074 0.0104 0.0112 0.007
Punctate Nuclear 0.0036 0.0005 0.0005 0.0007 0.0084 0.0004 0.0043 0.0004 0.0004 0.0002 0.0002 0.0003 0.0057 0.0007 0.0007 0.001 0.0028 0.0005
Vacuole 0.0085 0.0054 0.0123 0.0056 0.0013 0.0022 0.0089 0.0035 0.0043 0.012 0.0021 0.0029 0.0063 0.0077 0.0136 0.0086 0.0035 0.0036
Vacuole Periphery 0.0024 0.0015 0.0024 0.0026 0.0011 0.0013 0.0031 0.0015 0.0024 0.0102 0.0015 0.0021 0.0035 0.0022 0.0037 0.0052 0.0048 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.7165 21.0116 15.8802 27.178 16.0838 14.7909 20.6546 26.8188 25.2263 24.8766
Translational Efficiency 1.0088 0.9836 1.3637 0.8446 1.19 1.4799 0.9963 0.7459 0.8785 0.7718

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1619 1181 606 1695 184 109 228 107 1803 1290 834 1802

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 788.69 995.73 1417.95 1383.43 1052.83 1205.68 1310.98 1517.02 815.65 1013.47 1388.71 1391.36
Standard Deviation 102.38 150.17 170.77 203.42 129.79 190.76 218.14 298.68 132.38 164.71 190.97 212.64
Intensity Change Log 2 — 0.336296 0.846276 0.810719 — 0.195575 0.316373 0.526968 — 0.257531 0.567438 0.655380

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001730 0.001556 0.004976 0.009737 0.003745 0.001341 0.001452 0.005554 0.001936 0.001538 0.004013 0.009489
Bud Neck 0.001345 0.000825 0.003655 0.002617 0.002081 0.000684 0.004522 0.001858 0.001420 0.000813 0.003892 0.002572
Bud Site 0.003418 0.011210 0.037394 0.035156 0.009640 0.008410 0.038309 0.016916 0.004053 0.010973 0.037644 0.034073
Cell Periphery 0.000031 0.000034 0.000161 0.000285 0.000139 0.000057 0.000132 0.000365 0.000042 0.000036 0.000153 0.000290
Cytoplasm 0.004513 0.030570 0.030412 0.038242 0.007191 0.005984 0.034597 0.010769 0.004786 0.028492 0.031556 0.036610
Cytoplasmic Foci 0.239293 0.317674 0.284003 0.226096 0.241259 0.286403 0.336873 0.126314 0.239494 0.315032 0.298457 0.220171
Eisosomes 0.000027 0.000012 0.000015 0.000037 0.000019 0.000018 0.000012 0.000045 0.000026 0.000012 0.000014 0.000038
Endoplasmic Reticulum 0.000071 0.000212 0.000336 0.000273 0.000280 0.000507 0.000100 0.002210 0.000092 0.000237 0.000271 0.000388
Endosome 0.523599 0.443015 0.280684 0.337391 0.573913 0.456342 0.344906 0.309195 0.528734 0.444141 0.298241 0.335717
Golgi 0.129157 0.148950 0.129619 0.233418 0.108680 0.182959 0.049756 0.343667 0.127067 0.151823 0.107786 0.239965
Lipid Particles 0.012963 0.008523 0.019886 0.019752 0.005745 0.003051 0.009655 0.028792 0.012226 0.008060 0.017089 0.020289
Mitochondria 0.004368 0.000642 0.000066 0.001896 0.000709 0.001127 0.000038 0.004285 0.003995 0.000683 0.000059 0.002038
Mitotic Spindle 0.001797 0.007146 0.060271 0.015496 0.002737 0.008884 0.045243 0.001596 0.001893 0.007293 0.056162 0.014670
None 0.000049 0.000044 0.000012 0.000021 0.000093 0.000011 0.000010 0.000023 0.000054 0.000041 0.000011 0.000021
Nuclear Periphery 0.000016 0.000024 0.000354 0.000167 0.000071 0.000431 0.000238 0.000389 0.000022 0.000059 0.000322 0.000180
Nuclear Periphery Foci 0.000357 0.001157 0.007447 0.001156 0.001620 0.005841 0.005557 0.002270 0.000486 0.001553 0.006931 0.001222
Nucleolus 0.000110 0.000618 0.000032 0.000033 0.000100 0.000312 0.000118 0.000018 0.000109 0.000592 0.000056 0.000032
Nucleus 0.000232 0.001179 0.000085 0.000056 0.004152 0.000441 0.000098 0.000022 0.000632 0.001117 0.000089 0.000054
Peroxisomes 0.072809 0.011287 0.009182 0.019184 0.012025 0.010170 0.026673 0.008710 0.066606 0.011193 0.013964 0.018562
Vacuole 0.003006 0.011986 0.120008 0.049664 0.017940 0.019426 0.097988 0.115942 0.004530 0.012615 0.113988 0.053600
Vacuole Periphery 0.001108 0.003336 0.011399 0.009323 0.007861 0.007602 0.003725 0.021060 0.001797 0.003697 0.009301 0.010020

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.32 -2.15 -6.25 -6.43 -2.60 0.94 0.82 -0.43 -1.26 -1.17 0.71 -1.83 -6.10 -6.56 -3.51
Bud Neck 2.06 -6.60 -4.73 -8.04 3.11 1.55 -2.88 0.14 -1.99 3.53 2.46 -7.49 -4.45 -8.31 4.38
Bud Site -5.96 -11.26 -15.57 -10.58 0.63 0.24 -5.78 -1.35 -1.57 3.56 -5.60 -13.29 -15.36 -10.77 1.43
Cell Periphery -0.57 -10.37 -8.34 -8.29 -3.79 1.20 -0.10 -2.23 -3.90 -2.21 0.73 -5.78 -8.34 -8.78 -3.87
Cytoplasm -7.29 -7.24 -12.23 -2.25 -0.99 0.26 -4.18 -0.89 -1.08 3.19 -7.24 -8.50 -12.22 -2.49 -0.63
Cytoplasmic Foci -10.45 -4.49 2.09 12.24 5.96 -2.14 -4.27 5.99 7.23 9.93 -10.65 -6.44 3.17 13.28 8.75
Eisosomes 2.96 2.19 -1.59 -5.29 -4.43 0.17 1.07 -1.70 -1.69 -2.26 2.96 2.43 -1.89 -5.47 -4.93
Endoplasmic Reticulum -2.38 -3.74 -3.61 -0.66 0.75 -0.52 1.44 -1.17 -1.02 -1.27 -2.25 -3.36 -2.60 -1.15 -0.94
Endosome 7.99 23.86 21.62 11.77 -6.21 3.90 10.21 9.48 4.58 0.63 8.85 25.04 23.49 12.54 -4.99
Golgi -3.27 0.60 -14.88 -10.80 -11.58 -3.27 5.54 -8.09 -4.80 -10.84 -4.23 3.82 -16.59 -11.51 -17.02
Lipid Particles 2.46 -2.78 -3.47 -6.23 0.11 1.33 -1.70 -2.81 -3.17 -2.22 2.53 -2.49 -4.25 -6.96 -1.26
Mitochondria 4.22 4.97 2.72 -3.27 -5.46 -0.41 1.36 -0.93 -0.81 -1.11 4.15 5.05 2.31 -3.14 -5.09
Mitotic Spindle -3.90 -9.18 -8.68 -4.31 6.71 -1.13 -5.09 0.79 1.34 5.24 -4.03 -10.54 -8.59 -3.97 7.73
None 0.29 2.60 1.99 1.42 -3.62 1.01 0.96 0.87 -1.09 -0.89 0.63 2.68 2.15 1.36 -3.26
Nuclear Periphery -0.99 -6.74 -5.47 -5.03 3.38 -0.91 -3.06 -1.98 0.10 -0.78 -1.11 -7.96 -5.68 -2.82 3.30
Nuclear Periphery Foci -3.16 -7.47 -6.04 -0.04 6.59 -0.73 -2.44 -0.50 0.62 2.33 -2.03 -8.22 -4.36 0.58 7.30
Nucleolus -1.06 1.19 1.21 1.24 0.26 -0.68 -0.53 1.10 0.99 3.81 -1.10 0.76 1.34 1.29 3.20
Nucleus -1.42 1.05 1.33 1.67 1.95 1.12 1.18 1.24 0.99 2.60 -0.73 1.39 1.59 1.72 2.78
Peroxisomes 16.27 16.93 13.80 -4.23 -5.57 0.30 -3.12 0.54 0.34 2.45 16.07 14.76 13.50 -4.05 -2.21
Vacuole -8.29 -20.40 -22.23 -16.54 11.42 -0.23 -7.96 -5.89 -5.84 -0.33 -7.02 -22.13 -21.74 -17.21 11.37
Vacuole Periphery -6.00 -8.36 -13.62 -8.92 1.35 0.08 1.12 -2.33 -2.79 -4.08 -3.44 -7.45 -11.26 -8.95 -0.89
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
Localization
Cell Percentages endosome (81%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bro1

Bro1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bro1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available