Standard name | |
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Human Ortholog | |
Description | Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | – | 0 | 0 | 1 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 6 | – | 4 | 1 | 1 | 2 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 22 | 43 | 110 | 110 | 227 | 355 | 394 | 505 | – | 350 | 190 | 412 | 132 | 197 | – | 56 | 36 | 31 | 2 | 6 | 7 | 16 | 30 | 29 |
Endoplasmic Reticulum | 1 | 8 | 0 | 0 | 14 | 10 | 14 | 9 | – | 3 | 1 | 6 | 6 | 19 | – | 18 | 19 | 12 | 1 | 1 | 8 | 1 | 3 | 7 |
Endosome | 3 | 1 | 7 | 5 | 9 | 10 | 4 | 9 | – | 3 | 0 | 1 | 1 | 5 | – | 10 | 4 | 6 | 0 | 6 | 0 | 3 | 3 | 6 |
Golgi | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | – | 1 | 2 | 1 | 0 | 0 | – | 4 | 4 | 4 | 0 | 1 | 0 | 1 | 2 | 1 |
Mitochondria | 4 | 12 | 8 | 8 | 8 | 8 | 6 | 10 | – | 39 | 7 | 15 | 0 | 0 | – | 5 | 1 | 0 | 3 | 6 | 1 | 0 | 3 | 6 |
Nucleus | 18 | 11 | 16 | 16 | 0 | 11 | 26 | 50 | – | 34 | 10 | 21 | 9 | 0 | – | 142 | 114 | 74 | 2 | 14 | 2 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | – | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | – | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 2 |
Vac/Vac Membrane | 60 | 63 | 116 | 123 | 133 | 272 | 429 | 645 | – | 281 | 140 | 305 | 36 | 28 | – | 283 | 269 | 201 | 94 | 100 | 189 | 150 | 224 | 262 |
Unique Cell Count | 90 | 122 | 212 | 212 | 347 | 564 | 698 | 1029 | 582 | 301 | 645 | 168 | 239 | 409 | 372 | 271 | 109 | 148 | 212 | 182 | 280 | 332 | ||
Labelled Cell Count | 109 | 140 | 258 | 263 | 393 | 668 | 878 | 1241 | 717 | 353 | 766 | 188 | 254 | 521 | 450 | 332 | 109 | 148 | 212 | 182 | 280 | 332 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 6.4 | 6.1 | 6.1 | 8.6 | 8.6 | 8.3 | 8.9 | – | 7.3 | 8.9 | 8.9 | 6.5 | 6.9 | – | 12.7 | 12.9 | 12.7 | 7.1 | 7.1 | 7.0 |
Std Deviation (1e-4) | 1.5 | 1.9 | 2.0 | 2.0 | 2.4 | 2.3 | 2.2 | 2.4 | – | 3.3 | 2.9 | 2.9 | 1.2 | 1.8 | – | 3.8 | 4.0 | 3.9 | 1.7 | 1.8 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.49 | 0.48 | 0.43 | 0.53 | – | 0.26 | 0.54 | 0.54 | 0.09 | 0.17 | – | 1.05 | 1.07 | 1.05 | 0.22 | 0.22 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Cytoplasm | 0 | 3.2 | 2.8 | 1.2 | -0.7 | – | 2.1 | 2.5 | 3.1 | 5.4 | 6.9 | – | -10.2 | -11.3 | -9.7 |
Endoplasmic Reticulum | 0 | 3.0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 4.2 | – | 3.1 | 3.3 | 3.1 |
Endosome | -0.6 | -0.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -0.8 | – | -0.6 | 0 | -0.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Mitochondria | 0 | -1.0 | 0 | 0 | 0 | – | 1.5 | -1.0 | -1.1 | 0 | 0 | – | 0 | 0 | 0 |
Nucleus | 0 | -5.2 | -3.8 | -2.3 | -1.6 | – | -0.9 | -2.1 | -2.7 | -0.9 | -4.3 | – | 7.4 | 6.5 | 5.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Vacuole | 0.7 | -3.8 | -1.6 | 1.8 | 2.2 | – | -1.6 | -1.8 | -1.9 | -6.6 | -9.8 | – | 3.6 | 4.3 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1962 | 1.0703 | 0.6933 | 0.4051 | 0.8886 | 0.7949 | 0.3613 | 1.6794 | 0.9709 | 0.2758 | 0.8925 | 1.2271 | -0.0935 | 0.7171 | -0.2211 | -0.1773 | 0.025 | -0.0474 |
Actin | 0.019 | 0.0001 | 0.002 | 0.0015 | 0.0324 | 0.0002 | 0.0124 | 0.0028 | 0.0011 | 0.0039 | 0.0315 | 0.0012 | 0.0098 | 0 | 0.0003 | 0.0001 | 0.0233 | 0 |
Bud | 0.0022 | 0.0049 | 0.0049 | 0.0006 | 0.0124 | 0.0005 | 0.0007 | 0.0017 | 0.0033 | 0.0012 | 0.001 | 0.0003 | 0.0002 | 0.0009 | 0.0006 | 0.0006 | 0.0002 | 0.0002 |
Bud Neck | 0.0017 | 0.0015 | 0.0005 | 0.0045 | 0.0034 | 0.0014 | 0.0025 | 0.0019 | 0.0012 | 0.0015 | 0.0018 | 0.0015 | 0.0009 | 0.0003 | 0.0009 | 0.0003 | 0.0004 | 0.0017 |
Bud Periphery | 0.0018 | 0.0011 | 0.0049 | 0.0015 | 0.0057 | 0.0007 | 0.0011 | 0.0022 | 0.006 | 0.0026 | 0.0022 | 0.0011 | 0.0004 | 0.0021 | 0.0008 | 0.001 | 0.0003 | 0.0004 |
Bud Site | 0.0156 | 0.0037 | 0.0103 | 0.0032 | 0.0062 | 0.0008 | 0.0064 | 0.0036 | 0.0042 | 0.0023 | 0.0026 | 0.0005 | 0.0013 | 0.0018 | 0.0035 | 0.0006 | 0.0004 | 0.0003 |
Cell Periphery | 0.0014 | 0.0016 | 0.0005 | 0.0006 | 0.0009 | 0.0006 | 0.0023 | 0.0012 | 0.0011 | 0.0012 | 0.0014 | 0.0019 | 0.0014 | 0.001 | 0.0012 | 0.0003 | 0.0004 | 0.0009 |
Cytoplasm | 0.1177 | 0.1764 | 0.1279 | 0.1358 | 0.1124 | 0.1365 | 0.116 | 0.0895 | 0.0969 | 0.0715 | 0.0549 | 0.1145 | 0.1436 | 0.2676 | 0.1893 | 0.1045 | 0.1427 | 0.2421 |
Cytoplasmic Foci | 0.0426 | 0.0317 | 0.0667 | 0.0826 | 0.0639 | 0.046 | 0.0627 | 0.0225 | 0.0353 | 0.0365 | 0.0348 | 0.0365 | 0.0312 | 0.0349 | 0.0524 | 0.0317 | 0.0827 | 0.0368 |
Eisosomes | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0205 | 0.0236 | 0.0162 | 0.0082 | 0.0039 | 0.0142 | 0.035 | 0.0245 | 0.0333 | 0.0081 | 0.0413 | 0.036 | 0.0776 | 0.0359 | 0.0296 | 0.0047 | 0.0192 | 0.0209 |
Endosome | 0.1013 | 0.1305 | 0.2217 | 0.1907 | 0.2099 | 0.2146 | 0.1876 | 0.1675 | 0.2026 | 0.211 | 0.2013 | 0.1925 | 0.1796 | 0.123 | 0.2088 | 0.216 | 0.2763 | 0.1275 |
Golgi | 0.0068 | 0.0024 | 0.013 | 0.018 | 0.0509 | 0.0035 | 0.0074 | 0.0028 | 0.0047 | 0.0085 | 0.0026 | 0.0029 | 0.0035 | 0.0014 | 0.0032 | 0.0024 | 0.0079 | 0.0008 |
Lipid Particles | 0.0343 | 0.0098 | 0.0189 | 0.013 | 0.0534 | 0.012 | 0.0342 | 0.0042 | 0.0167 | 0.0108 | 0.0038 | 0.0122 | 0.0226 | 0.008 | 0.0106 | 0.014 | 0.0255 | 0.0036 |
Mitochondria | 0.0046 | 0.0026 | 0.0263 | 0.0135 | 0.0467 | 0.0104 | 0.0155 | 0.0042 | 0.0119 | 0.0175 | 0.0018 | 0.0103 | 0.0063 | 0.0012 | 0.0073 | 0.0012 | 0.0018 | 0.0006 |
None | 0.3715 | 0.3161 | 0.2724 | 0.3503 | 0.118 | 0.203 | 0.2094 | 0.1835 | 0.1814 | 0.1173 | 0.1132 | 0.0905 | 0.218 | 0.2408 | 0.1589 | 0.293 | 0.0727 | 0.1611 |
Nuclear Periphery | 0.0112 | 0.0132 | 0.0117 | 0.0074 | 0.0092 | 0.0068 | 0.0277 | 0.0227 | 0.0203 | 0.017 | 0.0074 | 0.0268 | 0.0381 | 0.0143 | 0.0147 | 0.0063 | 0.0163 | 0.0084 |
Nucleolus | 0.0014 | 0.002 | 0.0014 | 0.0072 | 0.0053 | 0.0013 | 0.0031 | 0.0019 | 0.0027 | 0.0016 | 0.0022 | 0.0017 | 0.0016 | 0.0022 | 0.0017 | 0.0006 | 0.0006 | 0.0008 |
Nucleus | 0.005 | 0.0067 | 0.0043 | 0.012 | 0.0068 | 0.0061 | 0.011 | 0.0099 | 0.0079 | 0.0092 | 0.0056 | 0.0151 | 0.011 | 0.0053 | 0.0101 | 0.0037 | 0.0027 | 0.0075 |
Peroxisomes | 0.0208 | 0.002 | 0.0068 | 0.0064 | 0.0585 | 0.0025 | 0.0063 | 0.0004 | 0.0062 | 0.0123 | 0.0037 | 0.0031 | 0.0022 | 0.0005 | 0.0011 | 0.0003 | 0.0468 | 0.0002 |
Punctate Nuclear | 0.0102 | 0.0011 | 0.0062 | 0.0035 | 0.0052 | 0.0031 | 0.0115 | 0.0009 | 0.0018 | 0.0011 | 0.0008 | 0.0019 | 0.0075 | 0.0043 | 0.0013 | 0.0007 | 0.0187 | 0.0009 |
Vacuole | 0.1931 | 0.2532 | 0.1579 | 0.1298 | 0.1683 | 0.3094 | 0.2208 | 0.4035 | 0.3221 | 0.3845 | 0.4296 | 0.3977 | 0.2194 | 0.2317 | 0.268 | 0.2767 | 0.2315 | 0.3548 |
Vacuole Periphery | 0.017 | 0.0156 | 0.0251 | 0.0097 | 0.0261 | 0.0265 | 0.0258 | 0.0485 | 0.0393 | 0.0803 | 0.0566 | 0.0517 | 0.0232 | 0.0226 | 0.0356 | 0.041 | 0.0296 | 0.0306 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.0359 | 16.543 | 20.7151 | 28.9392 | 21.7441 | 11.5597 | 32.6692 | 32.4568 | 37.1992 | 32.5903 |
Translational Efficiency | 1.1644 | 0.8115 | 0.8642 | 0.9091 | 0.7558 | 2.448 | 0.7965 | 0.8731 | 1.0132 | 0.7947 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1967 | 1663 | 2253 | 1264 | 1406 | 1666 | 1229 | 702 | 3373 | 3329 | 3482 | 1966 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 783.48 | 731.78 | 1161.89 | 898.22 | 680.55 | 712.75 | 729.31 | 902.18 | 740.57 | 722.26 | 1009.21 | 899.63 |
Standard Deviation | 131.06 | 102.62 | 173.91 | 186.64 | 116.62 | 92.46 | 122.32 | 193.16 | 135.14 | 98.13 | 259.98 | 189.00 |
Intensity Change Log 2 | — | -0.098486 | 0.568505 | 0.197172 | — | 0.066695 | 0.099831 | 0.406714 | — | -0.019345 | 0.369357 | 0.298372 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000014 | 0.000111 | 0.000059 | 0.001672 | 0.000036 | 0.001043 | 0.001367 | 0.000379 | 0.000023 | 0.000577 | 0.000521 | 0.001210 |
Bud Neck | 0.012586 | 0.017513 | 0.007558 | 0.019622 | 0.014997 | 0.033540 | 0.008711 | 0.011231 | 0.013591 | 0.025534 | 0.007965 | 0.016625 |
Bud Site | 0.005506 | 0.010504 | 0.006881 | 0.038680 | 0.005708 | 0.009713 | 0.022235 | 0.026033 | 0.005590 | 0.010108 | 0.012300 | 0.034164 |
Cell Periphery | 0.001121 | 0.000111 | 0.000095 | 0.000894 | 0.000169 | 0.000443 | 0.000169 | 0.000618 | 0.000724 | 0.000277 | 0.000121 | 0.000796 |
Cytoplasm | 0.050974 | 0.329574* | 0.031051 | 0.269033* | 0.194137* | 0.185510* | 0.515260* | 0.290296* | 0.110650* | 0.257477* | 0.201956* | 0.276626* |
Cytoplasmic Foci | 0.017039 | 0.094827 | 0.020245 | 0.035649 | 0.077249 | 0.122173 | 0.020901 | 0.065147 | 0.042137 | 0.108512 | 0.020476 | 0.046182 |
Eisosomes | 0.000090 | 0.000048 | 0.000009 | 0.000033 | 0.000055 | 0.000165 | 0.000037 | 0.000050 | 0.000075 | 0.000106 | 0.000018 | 0.000039 |
Endoplasmic Reticulum | 0.017811 | 0.002586 | 0.009409 | 0.006961 | 0.004168 | 0.004986 | 0.003017 | 0.015122 | 0.012124 | 0.003787 | 0.007153 | 0.009875 |
Endosome | 0.030159 | 0.039697 | 0.092378 | 0.045944 | 0.057838 | 0.075104 | 0.005861 | 0.049483 | 0.041696 | 0.057416 | 0.061841 | 0.047208 |
Golgi | 0.000359 | 0.001358 | 0.006277 | 0.004953 | 0.000786 | 0.005963 | 0.001326 | 0.005526 | 0.000537 | 0.003663 | 0.004529 | 0.005157 |
Lipid Particles | 0.005132 | 0.004016 | 0.003141 | 0.004384 | 0.005404 | 0.014695 | 0.002308 | 0.008601 | 0.005245 | 0.009360 | 0.002847 | 0.005890 |
Mitochondria | 0.000742 | 0.001848 | 0.001359 | 0.006381 | 0.001416 | 0.008390 | 0.001327 | 0.003061 | 0.001023 | 0.005122 | 0.001348 | 0.005196 |
Mitotic Spindle | 0.000064 | 0.000923 | 0.010313 | 0.023331 | 0.000756 | 0.004629 | 0.011220 | 0.010598 | 0.000352 | 0.002778 | 0.010633 | 0.018784 |
None | 0.004571 | 0.001995 | 0.000425 | 0.005326 | 0.001966 | 0.001506 | 0.007742 | 0.003875 | 0.003485 | 0.001750 | 0.003008 | 0.004808 |
Nuclear Periphery | 0.000875 | 0.000657 | 0.016130 | 0.001561 | 0.000736 | 0.001308 | 0.001712 | 0.001230 | 0.000817 | 0.000983 | 0.011041 | 0.001443 |
Nuclear Periphery Foci | 0.000388 | 0.000656 | 0.028622 | 0.004435 | 0.000579 | 0.000351 | 0.003137 | 0.008371 | 0.000468 | 0.000504 | 0.019627 | 0.005841 |
Nucleolus | 0.001756 | 0.001494 | 0.007221 | 0.001167 | 0.001745 | 0.002013 | 0.000877 | 0.000715 | 0.001751 | 0.001754 | 0.004982 | 0.001006 |
Nucleus | 0.027918 | 0.057026 | 0.014889 | 0.052178 | 0.042061 | 0.061387 | 0.280248* | 0.058587 | 0.033813 | 0.059208 | 0.108550 | 0.054466 |
Peroxisomes | 0.000536 | 0.003487 | 0.000614 | 0.002322 | 0.002040 | 0.009110 | 0.001068 | 0.003081 | 0.001163 | 0.006301 | 0.000774 | 0.002593 |
Vacuole | 0.811962* | 0.426096* | 0.640291* | 0.468156* | 0.581310* | 0.450379* | 0.110796 | 0.433129* | 0.715817* | 0.438248* | 0.453402* | 0.455649* |
Vacuole Periphery | 0.010399 | 0.005473 | 0.103032* | 0.007317 | 0.006843 | 0.007592 | 0.000683 | 0.004868 | 0.008917 | 0.006534 | 0.066907 | 0.006443 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.43 | -7.24 | -1.60 | -1.44 | -1.52 | -1.98 | -1.63 | -6.57 | 1.36 | 1.04 | -2.19 | -1.81 | -1.88 | -0.40 | -0.93 |
Bud Neck | -3.00 | 3.24 | -2.61 | 0.12 | -6.06 | -8.18 | 3.68 | 3.05 | 11.37 | -0.71 | -8.54 | 4.97 | -0.80 | 7.58 | -5.68 |
Bud Site | -4.12 | -1.86 | -8.42 | -6.49 | -7.90 | -3.79 | -4.65 | -6.10 | -4.48 | -1.51 | -5.56 | -4.84 | -10.32 | -7.93 | -7.35 |
Cell Periphery | 8.38 | 8.48 | 3.13 | -2.70 | -2.78 | -2.62 | 1.12 | -1.32 | 0.18 | -1.42 | 5.11 | 8.55 | 1.82 | -1.59 | -3.11 |
Cytoplasm | -35.98* | 3.99 | -29.54 | 3.03 | -32.26 | 1.24 | -23.60 | -8.46 | -9.65 | 10.12 | -25.41 | -11.84 | -26.14 | -5.34 | -15.42 |
Cytoplasmic Foci | -22.23 | -1.98 | -6.17 | 17.93 | -4.87 | -8.51 | 16.80 | 4.30 | 12.27 | -10.85 | -21.61 | 12.45 | 1.20 | 22.56 | -11.01 |
Eisosomes | 2.74 | 5.34 | 3.75 | 5.87 | -15.44 | -8.85 | 3.45 | 1.40 | 9.41 | -2.17 | -2.80 | 6.08 | 4.06 | 10.74 | -11.78 |
Endoplasmic Reticulum | 12.86 | 6.39 | 9.55 | -3.32 | 4.55 | -1.13 | 0.75 | -2.79 | -2.35 | -3.04 | 10.73 | 5.72 | 4.93 | -3.30 | 0.68 |
Endosome | -3.65 | -19.61 | 0.63 | 3.67 | 18.13 | -4.76 | 20.65 | 6.07 | 10.30 | -9.80 | -7.14 | -7.95 | 5.08 | 11.12 | 11.65 |
Golgi | -2.91 | -10.96 | -3.45 | -1.80 | 4.06 | -5.50 | 0.09 | -1.92 | 1.68 | -1.85 | -6.23 | -9.51 | -3.71 | 0.95 | 1.80 |
Lipid Particles | 1.83 | 2.76 | 1.80 | 0.26 | -0.87 | -7.01 | 4.96 | -0.73 | 4.76 | -3.21 | -5.44 | 4.86 | 0.77 | 5.52 | -3.00 |
Mitochondria | -4.09 | -4.22 | -2.76 | -1.71 | -2.09 | -7.03 | 0.76 | -0.52 | 5.08 | -0.91 | -8.06 | -1.36 | -2.63 | 2.34 | -2.28 |
Mitotic Spindle | -2.14 | -8.70 | -7.60 | -7.20 | -2.68 | -2.79 | -5.71 | -4.67 | -2.42 | 0.25 | -3.43 | -10.31 | -8.76 | -7.10 | -2.30 |
None | 2.44 | 4.07 | 0.76 | -1.58 | -3.31 | 0.63 | -4.43 | -0.87 | -1.19 | 1.58 | 2.52 | 1.62 | 0.02 | -2.06 | -1.31 |
Nuclear Periphery | 1.47 | -13.70 | -6.83 | -7.82 | 12.36 | -2.34 | -10.25 | -2.84 | 0.00 | 5.88 | -1.18 | -14.36 | -7.43 | -5.44 | 12.75 |
Nuclear Periphery Foci | -2.75 | -14.74 | -7.92 | -7.23 | 12.43 | 2.57 | -5.10 | -6.07 | -6.32 | -2.67 | -0.56 | -15.17 | -9.71 | -9.60 | 10.88 |
Nucleolus | 0.62 | -8.97 | 1.38 | 1.10 | 10.65 | -0.20 | 3.61 | 4.43 | 2.67 | 1.57 | 0.13 | -7.90 | 3.17 | 2.53 | 10.67 |
Nucleus | -9.47 | 4.54 | -11.27 | -3.84 | -15.37 | -4.80 | -33.44 | -5.31 | -1.91 | 26.52 | -10.27 | -21.74 | -11.95 | -4.09 | 10.43 |
Peroxisomes | -7.88 | -0.91 | -3.93 | 4.60 | -3.50 | -6.62 | 2.58 | 0.07 | 7.09 | -3.14 | -9.49 | 2.34 | -1.60 | 8.69 | -4.70 |
Vacuole | 38.94* | 21.91 | 31.65 | -3.88 | 16.21 | 10.50 | 39.76* | 8.69 | 0.02 | -22.56 | 34.95* | 31.83 | 28.09 | -2.78 | 0.32 |
Vacuole Periphery | 7.09 | -36.02* | 7.80 | 1.67 | 38.75* | -1.46 | 17.13 | 5.06 | 5.92 | -3.83 | 5.21 | -31.10 | 9.14 | 5.25 | 33.81* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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