Standard name
Human Ortholog
Description Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.35 0.52 0.52 0.65 0.63 0.56 0.49 – 0.6 0.63 0.64 0.79 0.82 – 0.14 0.1 0.11 0 0 0 0.09 0.11 0.09
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 – 0 0 0 0 0.08 – 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0 0 0 0 – 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0.2 0.09 0.08 0.08 0 0 0 0.05 – 0.06 0 0 0.05 0 – 0.35 0.31 0.27 0 0.1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.67 0.52 0.55 0.58 0.38 0.48 0.61 0.63 – 0.48 0.47 0.47 0.21 0.12 – 0.69 0.72 0.74 0.87 0.68 0.89 0.83 0.8 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 2 – 0 0 1 0 2 – 0 0 0 0 0 0 0 1 0
Bud 0 1 0 0 1 0 3 6 – 4 1 1 2 0 – 0 0 0 0 1 1 3 4 5
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 1 0 1 1 3 – 0 0 0 0 0 0 0 0 0
Cytoplasm 22 43 110 110 227 355 394 505 – 350 190 412 132 197 – 56 36 31 2 6 7 16 30 29
Endoplasmic Reticulum 1 8 0 0 14 10 14 9 – 3 1 6 6 19 – 18 19 12 1 1 8 1 3 7
Endosome 3 1 7 5 9 10 4 9 – 3 0 1 1 5 – 10 4 6 0 6 0 3 3 6
Golgi 0 0 1 1 0 1 1 1 – 1 2 1 0 0 – 4 4 4 0 1 0 1 2 1
Mitochondria 4 12 8 8 8 8 6 10 – 39 7 15 0 0 – 5 1 0 3 6 1 0 3 6
Nucleus 18 11 16 16 0 11 26 50 – 34 10 21 9 0 – 142 114 74 2 14 2 0 1 1
Nuclear Periphery 0 0 0 0 0 0 1 4 – 1 1 1 0 0 – 0 0 0 0 2 0 0 0 1
Nucleolus 1 1 0 0 0 1 0 0 – 0 1 1 0 0 – 2 2 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 1 0 – 1 0 2 0 1 0 1 0 2
Vac/Vac Membrane 60 63 116 123 133 272 429 645 – 281 140 305 36 28 – 283 269 201 94 100 189 150 224 262
Unique Cell Count 90 122 212 212 347 564 698 1029 582 301 645 168 239 409 372 271 109 148 212 182 280 332
Labelled Cell Count 109 140 258 263 393 668 878 1241 717 353 766 188 254 521 450 332 109 148 212 182 280 332


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 6.4 6.1 6.1 8.6 8.6 8.3 8.9 – 7.3 8.9 8.9 6.5 6.9 – 12.7 12.9 12.7 7.1 7.1 7.0
Std Deviation (1e-4) 1.5 1.9 2.0 2.0 2.4 2.3 2.2 2.4 – 3.3 2.9 2.9 1.2 1.8 – 3.8 4.0 3.9 1.7 1.8 1.6
Intensity Change (Log2) – – – 0.0 0.49 0.48 0.43 0.53 – 0.26 0.54 0.54 0.09 0.17 – 1.05 1.07 1.05 0.22 0.22 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Cytoplasm 0 3.2 2.8 1.2 -0.7 – 2.1 2.5 3.1 5.4 6.9 – -10.2 -11.3 -9.7
Endoplasmic Reticulum 0 3.0 0 0 0 – 0 0 0 0 4.2 – 3.1 3.3 3.1
Endosome -0.6 -0.5 0 0 0 – 0 0 0 0 -0.8 – -0.6 0 -0.7
Golgi 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Mitochondria 0 -1.0 0 0 0 – 1.5 -1.0 -1.1 0 0 – 0 0 0
Nucleus 0 -5.2 -3.8 -2.3 -1.6 – -0.9 -2.1 -2.7 -0.9 -4.3 – 7.4 6.5 5.5
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Vacuole 0.7 -3.8 -1.6 1.8 2.2 – -1.6 -1.8 -1.9 -6.6 -9.8 – 3.6 4.3 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1962 1.0703 0.6933 0.4051 0.8886 0.7949 0.3613 1.6794 0.9709 0.2758 0.8925 1.2271 -0.0935 0.7171 -0.2211 -0.1773 0.025 -0.0474
Actin 0.019 0.0001 0.002 0.0015 0.0324 0.0002 0.0124 0.0028 0.0011 0.0039 0.0315 0.0012 0.0098 0 0.0003 0.0001 0.0233 0
Bud 0.0022 0.0049 0.0049 0.0006 0.0124 0.0005 0.0007 0.0017 0.0033 0.0012 0.001 0.0003 0.0002 0.0009 0.0006 0.0006 0.0002 0.0002
Bud Neck 0.0017 0.0015 0.0005 0.0045 0.0034 0.0014 0.0025 0.0019 0.0012 0.0015 0.0018 0.0015 0.0009 0.0003 0.0009 0.0003 0.0004 0.0017
Bud Periphery 0.0018 0.0011 0.0049 0.0015 0.0057 0.0007 0.0011 0.0022 0.006 0.0026 0.0022 0.0011 0.0004 0.0021 0.0008 0.001 0.0003 0.0004
Bud Site 0.0156 0.0037 0.0103 0.0032 0.0062 0.0008 0.0064 0.0036 0.0042 0.0023 0.0026 0.0005 0.0013 0.0018 0.0035 0.0006 0.0004 0.0003
Cell Periphery 0.0014 0.0016 0.0005 0.0006 0.0009 0.0006 0.0023 0.0012 0.0011 0.0012 0.0014 0.0019 0.0014 0.001 0.0012 0.0003 0.0004 0.0009
Cytoplasm 0.1177 0.1764 0.1279 0.1358 0.1124 0.1365 0.116 0.0895 0.0969 0.0715 0.0549 0.1145 0.1436 0.2676 0.1893 0.1045 0.1427 0.2421
Cytoplasmic Foci 0.0426 0.0317 0.0667 0.0826 0.0639 0.046 0.0627 0.0225 0.0353 0.0365 0.0348 0.0365 0.0312 0.0349 0.0524 0.0317 0.0827 0.0368
Eisosomes 0.0001 0.0002 0.0002 0.0001 0.0004 0 0.0005 0 0.0001 0.0001 0.0001 0.0001 0.0005 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0205 0.0236 0.0162 0.0082 0.0039 0.0142 0.035 0.0245 0.0333 0.0081 0.0413 0.036 0.0776 0.0359 0.0296 0.0047 0.0192 0.0209
Endosome 0.1013 0.1305 0.2217 0.1907 0.2099 0.2146 0.1876 0.1675 0.2026 0.211 0.2013 0.1925 0.1796 0.123 0.2088 0.216 0.2763 0.1275
Golgi 0.0068 0.0024 0.013 0.018 0.0509 0.0035 0.0074 0.0028 0.0047 0.0085 0.0026 0.0029 0.0035 0.0014 0.0032 0.0024 0.0079 0.0008
Lipid Particles 0.0343 0.0098 0.0189 0.013 0.0534 0.012 0.0342 0.0042 0.0167 0.0108 0.0038 0.0122 0.0226 0.008 0.0106 0.014 0.0255 0.0036
Mitochondria 0.0046 0.0026 0.0263 0.0135 0.0467 0.0104 0.0155 0.0042 0.0119 0.0175 0.0018 0.0103 0.0063 0.0012 0.0073 0.0012 0.0018 0.0006
None 0.3715 0.3161 0.2724 0.3503 0.118 0.203 0.2094 0.1835 0.1814 0.1173 0.1132 0.0905 0.218 0.2408 0.1589 0.293 0.0727 0.1611
Nuclear Periphery 0.0112 0.0132 0.0117 0.0074 0.0092 0.0068 0.0277 0.0227 0.0203 0.017 0.0074 0.0268 0.0381 0.0143 0.0147 0.0063 0.0163 0.0084
Nucleolus 0.0014 0.002 0.0014 0.0072 0.0053 0.0013 0.0031 0.0019 0.0027 0.0016 0.0022 0.0017 0.0016 0.0022 0.0017 0.0006 0.0006 0.0008
Nucleus 0.005 0.0067 0.0043 0.012 0.0068 0.0061 0.011 0.0099 0.0079 0.0092 0.0056 0.0151 0.011 0.0053 0.0101 0.0037 0.0027 0.0075
Peroxisomes 0.0208 0.002 0.0068 0.0064 0.0585 0.0025 0.0063 0.0004 0.0062 0.0123 0.0037 0.0031 0.0022 0.0005 0.0011 0.0003 0.0468 0.0002
Punctate Nuclear 0.0102 0.0011 0.0062 0.0035 0.0052 0.0031 0.0115 0.0009 0.0018 0.0011 0.0008 0.0019 0.0075 0.0043 0.0013 0.0007 0.0187 0.0009
Vacuole 0.1931 0.2532 0.1579 0.1298 0.1683 0.3094 0.2208 0.4035 0.3221 0.3845 0.4296 0.3977 0.2194 0.2317 0.268 0.2767 0.2315 0.3548
Vacuole Periphery 0.017 0.0156 0.0251 0.0097 0.0261 0.0265 0.0258 0.0485 0.0393 0.0803 0.0566 0.0517 0.0232 0.0226 0.0356 0.041 0.0296 0.0306

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0359 16.543 20.7151 28.9392 21.7441 11.5597 32.6692 32.4568 37.1992 32.5903
Translational Efficiency 1.1644 0.8115 0.8642 0.9091 0.7558 2.448 0.7965 0.8731 1.0132 0.7947

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (88%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ydc1

Ydc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available