Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0.05 0 0.06 0 0.1 0.05 0 0 0 0 0 0 0.05 0.09 0.05 0 0.05 0 0.05 0.05 0.05 0
Golgi 0 0 0.13 0.19 0.22 0.06 0 0 0 0 0 0 0.14 0.21 0.22 0.32 0.05 0.07 0 0 0.06 0.05
Mitochondria 0.92 0.92 0.71 0.83 0.67 0.8 0.69 0.81 0.9 0.87 0.89 0.86 0.84 0.75 0.71 0 0.79 0.76 0.77 0.72 0.71 0.74
Nucleus 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.07 0.08 0.06 0.09 0.11 0.05 0.07 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.08 0.06 0.14 0.1 0.11 0.08 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.05 0
SpindlePole 0 0 0.08 0 0 0 0.07 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.11 0.08 0.08 0.14 0.16 0.13 0.14 0.07 0 0.06 0.06 0.14 0.1 0.13 0.2 0 0.07 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 8 4 0 0 0 2 3 1 0 1 7 9 0 1 0 0 0 0 1 2
Bud 0 0 2 4 2 5 4 4 3 0 4 5 5 4 3 1 1 0 0 1 0 1
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 2 4 1 0 0 1 2 0 0 0 0
Cell Periphery 10 5 5 5 3 1 2 3 0 0 3 3 4 8 2 2 0 0 0 0 0 0
Cytoplasm 1 7 3 3 6 6 9 8 2 3 4 2 2 3 8 5 0 2 0 3 2 1
Endoplasmic Reticulum 1 1 1 2 1 1 3 1 1 2 0 2 4 10 15 0 0 0 0 1 1 1
Endosome 10 0 9 3 8 10 8 9 0 0 2 5 8 19 13 1 10 4 9 10 15 12
Golgi 3 1 20 21 17 11 3 0 0 1 2 2 23 43 62 8 8 10 7 9 17 16
Mitochondria 175 133 109 92 52 152 147 177 111 138 246 241 133 153 197 0 151 110 137 154 213 234
Nucleus 1 0 0 0 2 0 12 8 3 3 3 4 0 0 3 0 0 0 0 3 3 3
Nuclear Periphery 9 6 3 1 2 14 18 14 11 18 15 21 3 3 9 1 1 1 0 2 3 4
Nucleolus 6 0 3 1 1 9 17 12 17 16 29 23 3 0 4 1 0 0 0 2 0 3
Peroxisomes 0 0 18 0 1 3 1 3 0 0 1 4 6 4 6 0 4 1 15 8 14 14
SpindlePole 0 0 12 2 1 7 14 4 5 6 6 8 7 9 5 2 2 0 1 1 3 1
Vac/Vac Membrane 45 16 12 9 11 30 28 31 9 7 16 18 23 20 37 5 7 10 2 10 14 10
Unique Cell Count 191 144 154 111 78 191 213 218 123 158 275 280 159 205 279 25 192 145 179 213 300 315
Labelled Cell Count 263 170 206 147 107 251 270 277 165 195 332 341 228 285 364 27 192 145 179 213 300 315


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 5.1 7.1 6.8 7.7 6.7 6.5 6.6 5.6 6.0 5.8 6.4 6.7 6.7 6.5 6.8 7.3 7.7 7.9
Std Deviation (1e-4) 1.3 1.0 1.4 1.3 1.7 1.3 1.3 1.1 1.1 1.5 1.2 1.3 1.1 1.0 1.1 1.4 1.5 1.4 1.8
Intensity Change (Log2) -0.06 0.12 -0.07 -0.13 -0.1 -0.34 -0.23 -0.29 -0.15 -0.08 -0.07 -0.12 -0.05 0.05 0.12 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.6 0 0 0 0 0 0 0 0 -0.3 -0.4 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.3 0 0
Cytoplasm 0 0 0 1.2 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.5 0
Endosome -1.2 1.2 -0.2 -0.9 -0.8 0 0 0 0 -0.3 1.2 -0.5 0
Golgi 1.3 1.7 -2.3 -4.5 -5.5 -4.1 -4.4 -5.5 -5.6 0.4 2.0 2.3 0
Mitochondria 2.3 -0.6 1.9 -0.4 2.3 4.0 3.6 4.9 3.9 2.7 0.8 0 -6.7
Nucleus 0 0 0 3.0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.3 2.6 2.0 2.6 3.3 1.7 2.4 0 0 0 0
Nucleolus 0 0 1.4 2.5 1.7 3.8 3.0 3.3 2.6 0 0 0 0
Peroxisomes -3.7 -2.7 -3.9 -4.8 -4.2 -3.9 -4.4 -5.5 -4.7 -2.6 -3.8 -4.2 0
SpindlePole -2.2 0 -1.7 -0.4 -2.8 -1.3 -1.5 -2.8 -2.3 -1.3 -1.4 -3.1 0
Vacuole 0.1 1.5 2.2 1.6 1.9 -0.1 -1.2 -0.8 -0.5 1.9 0.6 1.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4894 2.3104 2.3263 1.9666 1.4834 2.4118 6.1375 5.933 6.1395 4.8778 6.1154 6.1393 4.1536 4.1724 4.2302 3.9108 3.4623 4.0178
Actin 0.0055 0.0038 0.0031 0.0022 0.0016 0.0013 0.0266 0.0131 0.0176 0.0028 0.0266 0.0081 0.0166 0.0039 0.0247 0.0076 0.0014 0.0072
Bud 0.0002 0.0015 0.0005 0.0005 0.0001 0.0002 0.0004 0.0007 0.0005 0.0002 0.0005 0.0002 0.0003 0.0006 0.0009 0.0006 0.0001 0.0003
Bud Neck 0.0003 0.0002 0.0001 0.0002 0 0.0025 0.0009 0.0005 0.0003 0 0.0003 0.0012 0.0003 0.0002 0.0009 0.0018 0.0001 0.0011
Bud Periphery 0.0003 0.0026 0.0012 0.0005 0.0002 0.0004 0.0009 0.0008 0.0014 0.0006 0.0011 0.0006 0.0012 0.0016 0.0013 0.0012 0.0002 0.0018
Bud Site 0.0004 0.0088 0.0013 0.0029 0.0001 0.0019 0.0014 0.0126 0.0036 0.0001 0.0009 0.0003 0.001 0.0024 0.0023 0.0027 0.0002 0.0003
Cell Periphery 0.0001 0.0002 0.0011 0.0001 0.0001 0.0002 0.0004 0.0036 0.0008 0 0.0007 0.0002 0.0012 0.0004 0.0003 0.0004 0.0001 0.0007
Cytoplasm 0.0017 0.0003 0.001 0.0024 0 0.0001 0.0021 0.0004 0.0004 0 0.0013 0.0007 0.0005 0.0011 0.0032 0.003 0.0002 0.0013
Cytoplasmic Foci 0.0094 0.0128 0.0046 0.017 0.0028 0.0034 0.0179 0.0269 0.0098 0.0027 0.048 0.0118 0.0102 0.0123 0.0217 0.0277 0.0052 0.0138
Eisosomes 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0014 0.001 0.0004 0.0001 0.0003 0.0003 0.0005 0.0004 0.0004 0.0002 0.0001 0.0005
Endoplasmic Reticulum 0.0006 0.0001 0.0007 0.0002 0.0001 0.0001 0.0016 0.0001 0.0002 0 0.0003 0.0005 0.0008 0.0011 0.0011 0.002 0.0003 0.0006
Endosome 0.019 0.0398 0.0154 0.0206 0.0087 0.0131 0.0374 0.0219 0.0121 0.0014 0.0231 0.0117 0.0215 0.0252 0.0201 0.0443 0.0277 0.0188
Golgi 0.0306 0.0752 0.0284 0.0349 0.0255 0.0302 0.0423 0.0648 0.0225 0.0044 0.0602 0.0234 0.0265 0.0326 0.0364 0.0398 0.0702 0.0444
Lipid Particles 0.0196 0.0102 0.0089 0.0081 0.0085 0.0061 0.0241 0.0077 0.0103 0.0096 0.0165 0.0174 0.0255 0.0156 0.0137 0.0063 0.0134 0.0064
Mitochondria 0.8831 0.796 0.9122 0.8858 0.9353 0.9256 0.7622 0.7515 0.8732 0.8537 0.6897 0.8735 0.8342 0.8602 0.8473 0.831 0.8698 0.8718
None 0.0003 0.0001 0.0018 0.0002 0 0 0.0063 0.0002 0.0005 0.0001 0.0013 0.0035 0.0013 0.0127 0.0009 0.0019 0.0004 0.0024
Nuclear Periphery 0.0015 0.0001 0.0005 0 0 0.0001 0.002 0 0.0004 0 0.0003 0.0004 0.01 0.0003 0.0005 0.0138 0.0004 0.0003
Nucleolus 0.0002 0.0007 0.0002 0 0 0.0001 0.0004 0.0001 0.0002 0 0.0001 0.0001 0.0005 0.0003 0.0003 0.0003 0.0001 0.0003
Nucleus 0.0006 0.0002 0.0002 0.0001 0 0.0001 0.0007 0 0.0002 0 0.0001 0.0001 0.0007 0.0002 0.0003 0.0008 0.0001 0.0002
Peroxisomes 0.0218 0.0418 0.0136 0.0228 0.0152 0.0113 0.065 0.093 0.0406 0.124 0.1258 0.043 0.0379 0.0244 0.0147 0.0045 0.007 0.0213
Punctate Nuclear 0.0018 0.0001 0 0.0001 0 0 0.001 0.0002 0.0006 0 0.0014 0.0007 0.0016 0.0001 0.0005 0.003 0.0002 0.0012
Vacuole 0.0011 0.0029 0.002 0.0007 0.0004 0.0012 0.0023 0.0005 0.0021 0.0001 0.0005 0.0007 0.0045 0.0026 0.003 0.0033 0.001 0.0018
Vacuole Periphery 0.0016 0.0026 0.0028 0.0007 0.001 0.002 0.0028 0.0006 0.0025 0.0003 0.0011 0.0018 0.0034 0.0021 0.0057 0.0037 0.0018 0.0033

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.3856 7.9227 7.8386 9.4885 17.2363 10.3341 9.6795 11.2638 21.8722 11.4655
Translational Efficiency 1.7184 1.3041 1.2981 2.1176 0.6094 1.079 1.0827 1.2014 0.6283 0.8957

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2133 1436 2084 1426 1096 1734 473 1741 3229 3170 2557 3167

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 794.39 883.62 855.75 911.34 776.61 934.87 940.49 918.22 788.36 911.65 871.43 915.12
Standard Deviation 103.10 132.65 107.82 125.93 105.67 116.99 109.57 153.22 104.32 126.92 113.04 141.63
Intensity Change Log 2 0.153579 0.107342 0.198142 0.267575 0.276222 0.241650 0.211058 0.193297 0.219814

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000557 0.004409 0.002459 0.004065 0.001008 0.000629 0.003765 0.004488 0.000710 0.002341 0.002700 0.004297
Bud Neck 0.001242 0.002488 0.008113 0.006266 0.002671 0.001993 0.016583 0.005584 0.001727 0.002217 0.009680 0.005891
Bud Site 0.003722 0.010824 0.034346 0.036951 0.006305 0.003518 0.037817 0.033318 0.004599 0.006828 0.034988 0.034954
Cell Periphery 0.000356 0.000466 0.000345 0.000298 0.000385 0.000401 0.000292 0.000777 0.000366 0.000430 0.000336 0.000562
Cytoplasm 0.000819 0.002402 0.006418 0.013016 0.001852 0.000987 0.015448 0.011247 0.001170 0.001628 0.008089 0.012043
Cytoplasmic Foci 0.017374 0.036810 0.023890 0.085493 0.032475 0.023147 0.116890 0.061963 0.022500 0.029336 0.041093 0.072558
Eisosomes 0.000040 0.000086 0.000061 0.000093 0.000055 0.000045 0.000135 0.000100 0.000045 0.000064 0.000075 0.000097
Endoplasmic Reticulum 0.000321 0.000341 0.000472 0.002046 0.000502 0.000170 0.003779 0.001212 0.000382 0.000247 0.001084 0.001587
Endosome 0.044457 0.059664 0.101860 0.108968 0.056826 0.065580 0.152327 0.114258 0.048655 0.062900 0.111195 0.111876
Golgi 0.047387 0.061832 0.040947 0.132171* 0.061088 0.053611 0.085034 0.129518* 0.052038 0.057335 0.049102 0.130713*
Lipid Particles 0.002596 0.004378 0.000657 0.002755 0.007116 0.007737 0.009440 0.003978 0.004130 0.006215 0.002282 0.003427
Mitochondria 0.836711* 0.743227* 0.636002* 0.393395* 0.766136* 0.797482* 0.218267* 0.498346* 0.812757* 0.772904* 0.558728* 0.451090*
Mitotic Spindle 0.003258 0.006508 0.052282 0.070821 0.009277 0.004296 0.113782* 0.039469 0.005301 0.005298 0.063659 0.053586
None 0.000190 0.000183 0.000314 0.000502 0.000307 0.000186 0.000743 0.000737 0.000230 0.000184 0.000393 0.000631
Nuclear Periphery 0.000057 0.000059 0.000170 0.000427 0.000143 0.000117 0.001738 0.000436 0.000086 0.000091 0.000460 0.000432
Nuclear Periphery Foci 0.000095 0.000187 0.000238 0.000881 0.000564 0.000201 0.002586 0.000998 0.000254 0.000195 0.000672 0.000945
Nucleolus 0.000235 0.000141 0.000458 0.000481 0.000305 0.000560 0.004324 0.000352 0.000259 0.000370 0.001173 0.000410
Nucleus 0.000155 0.000117 0.000823 0.001258 0.000389 0.000398 0.020864 0.000651 0.000234 0.000271 0.004530 0.000924
Peroxisomes 0.025995 0.053626 0.040546 0.033171 0.034746 0.019282 0.095913 0.042524 0.028966 0.034840 0.050788 0.038313
Vacuole 0.005070 0.006559 0.033786 0.070642 0.006698 0.009623 0.071598 0.031064 0.005623 0.008235 0.040780 0.048885
Vacuole Periphery 0.009361 0.005695 0.015811 0.036300 0.011151 0.010036 0.028675 0.018980 0.009969 0.008069 0.018191 0.026779

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.97 -2.96 -5.37 0.31 -1.80 1.94 -3.66 -5.22 -5.85 -0.64 -4.44 -3.69 -7.66 -3.39 -2.26
Bud Neck -2.38 -9.45 -6.98 -4.69 1.89 1.07 -5.84 -3.65 -4.85 4.54 -1.29 -10.70 -8.03 -6.74 4.45
Bud Site -4.38 -13.69 -13.32 -9.22 -0.83 2.42 -7.52 -11.37 -13.49 0.93 -2.42 -15.33 -17.96 -15.93 -0.01
Cell Periphery -1.08 0.18 1.04 1.87 1.33 -0.13 1.11 -2.37 -2.22 -3.30 -0.85 0.61 -2.13 -1.28 -2.70
Cytoplasm -3.29 -9.48 -8.88 -7.54 -4.64 2.20 -6.21 -7.80 -8.82 1.93 -1.47 -10.86 -12.13 -11.54 -3.70
Cytoplasmic Foci -8.41 -4.18 -20.75 -13.10 -18.49 3.78 -13.53 -9.61 -14.51 8.83 -4.26 -9.88 -23.52 -19.28 -12.46
Eisosomes -6.88 -7.22 -11.15 -1.02 -6.63 2.89 -9.70 -7.90 -10.45 4.41 -5.10 -10.51 -14.09 -7.40 -5.07
Endoplasmic Reticulum -0.17 -1.75 -6.97 -6.77 -6.43 4.65 -4.77 -1.91 -2.82 3.40 1.96 -4.86 -5.16 -5.79 -1.73
Endosome -3.96 -13.80 -15.03 -10.38 -1.37 -1.88 -10.74 -11.17 -10.13 4.10 -4.89 -16.60 -19.39 -14.41 -0.23
Golgi -3.62 2.19 -15.22 -11.73 -17.11 1.63 -3.05 -11.86 -14.37 -6.18 -1.82 1.26 -20.16 -18.51 -21.40
Lipid Particles -1.87 3.60 -0.34 1.57 -3.96 -0.29 -1.39 2.01 2.47 3.55 -2.08 2.74 0.88 2.91 -1.86
Mitochondria 8.67 20.62 39.74* 26.93* 19.74* -2.74 34.60* 20.73 26.45 -18.09* 5.22 28.19 43.66* 37.42* 10.66
Mitotic Spindle -4.02 -17.83 -15.94 -15.06 -3.72 3.90 -11.05 -10.43 -12.67 7.56 0.00 -20.22 -19.57 -19.55 2.62
None 0.42 -3.58 -1.75 -1.82 -1.21 3.03 -2.07 -1.31 -1.72 -0.04 2.18 -3.34 -2.11 -2.37 -1.34
Nuclear Periphery -0.12 -4.67 -7.29 -7.44 -4.74 0.44 -3.70 -1.60 -1.79 2.84 -0.20 -4.49 -3.42 -3.40 0.30
Nuclear Periphery Foci -1.92 -3.05 -6.44 -5.61 -4.65 1.78 -4.59 -1.06 -2.06 2.98 0.86 -4.18 -3.00 -3.40 -0.87
Nucleolus 1.01 -1.22 -1.40 -2.89 -0.16 -0.90 -4.63 -0.44 0.76 4.59 -0.46 -4.34 -1.31 -0.44 3.83
Nucleus 0.73 -3.78 -5.50 -5.85 -1.71 0.01 -5.59 -2.20 -1.78 5.49 -0.29 -6.07 -5.94 -5.08 4.96
Peroxisomes -6.20 -4.53 -2.23 4.36 2.09 4.50 -7.54 -1.82 -7.39 6.74 -2.16 -7.23 -3.64 -1.30 4.07
Vacuole -1.66 -16.40 -18.82 -18.14 -9.55 -2.66 -11.59 -12.58 -10.86 7.12 -3.77 -19.67 -22.57 -20.77 -2.92
Vacuole Periphery 4.04 -5.60 -13.08 -15.45 -9.71 0.84 -5.40 -5.35 -6.97 2.90 2.41 -7.44 -13.93 -16.29 -6.33
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Dpc25

Dpc25


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dpc25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available