Standard name
Human Ortholog
Description Arginase, catabolizes arginine to ornithine and urea; expression responds to both induction by arginine and nitrogen catabolite repression; disruption decreases production of carcinogen ethyl carbamate during wine fermentation and also enhances freeze tolerance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.05 0.07 0.07 0.09 0 0 0 0.17 0.21 0.13 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.99 0.98 0.98 0.9 0.88 0.92 0.87 0.86 0.76 0.99 0.99 0.99 0.9 0.91 0.95 0.97 0.95 0.95 0.94 0.94 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.1 0.05 0.07 0 0.07 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 2 2 3 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 1 1 0 1 2 4 1 1 0 0 1 0 0 0 0 1 0 1 4 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 3 4 8 15 11 15 7 13 0 3 6 56 33 27 0 0 0 0 0 0
Cytoplasm 308 138 99 162 250 177 211 210 206 193 83 115 450 627 628 301 143 201 278 126 88 222 517 558
Endoplasmic Reticulum 1 0 0 1 4 3 17 23 11 15 4 11 2 2 3 20 10 7 1 0 0 1 6 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 2 0 3 1 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0
Nucleus 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1 1 1 1 3 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 0 0 1 1 0 3 3 11 2 2 1 0 1 0 0 0 2 1 3 3
Unique Cell Count 310 139 99 163 255 181 235 240 224 222 96 151 453 632 633 333 157 211 287 133 94 236 549 580
Labelled Cell Count 310 139 99 163 258 185 238 252 232 235 101 160 455 635 639 378 187 235 287 133 94 236 549 580


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 152.3 221.5 147.0 131.9 121.9 90.4 76.7 71.4 57.3 58.2 45.0 48.4 139.2 128.3 125.2 482.4 511.2 475.6 152.3 141.3 134.7
Std Deviation (1e-4) 32.3 55.6 45.9 41.4 31.5 29.0 23.0 21.8 18.2 17.4 18.3 13.9 36.5 34.2 31.2 104.6 105.5 134.5 41.6 40.8 39.7
Intensity Change (Log2) – – – -0.16 -0.27 -0.7 -0.94 -1.04 -1.36 -1.34 -1.71 -1.6 -0.08 -0.2 -0.23 1.71 1.8 1.69 0.05 -0.06 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 3.0 0 0 0 4.4 4.9 3.7
Cytoplasm -0.8 -1.4 -1.5 -3.3 -3.7 -2.9 -3.8 -3.8 -5.3 -0.8 -0.9 -0.9 -3.2 -3.1 -2.2
Endoplasmic Reticulum 0 0 0 2.7 3.2 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 43.3378 55.0847 49.0838 48.7228 39.955 53.3273 55.6135 64.1655 57.7925 56.9654 56.0018 62.8988 53.8234 62.7049 56.9227 48.0254 40.1954 59.7493
Actin 0.0076 0.0001 0.0023 0.002 0.0024 0.0015 0.0055 0.0014 0.0012 0.0003 0.0002 0.0003 0.0023 0.0001 0.0103 0.0084 0.0092 0.0001
Bud 0.0033 0 0.0013 0.0001 0.0004 0.0006 0.0008 0.0001 0.0001 0.0009 0 0.0001 0.0002 0 0.0003 0.0008 0.0013 0
Bud Neck 0.0005 0.0001 0.0002 0.0003 0.0007 0.0005 0.0004 0.0003 0.0001 0.0002 0.0002 0.0007 0.0001 0.0001 0.0009 0.0004 0.0005 0.0003
Bud Periphery 0.0015 0 0.001 0.0001 0.0006 0.0003 0.0006 0 0.0001 0.0006 0 0.0001 0.0001 0 0.0002 0.0006 0.0013 0
Bud Site 0.0015 0 0.0007 0.0002 0.0007 0.0001 0.0013 0.0022 0.0003 0.0002 0 0 0.0008 0 0.002 0.0009 0.0043 0
Cell Periphery 0.0004 0 0.0001 0 0.0003 0.0001 0.0004 0.0001 0.0001 0.0002 0 0 0.0001 0 0.0001 0.0002 0.0004 0
Cytoplasm 0.8529 0.9912 0.9289 0.9277 0.9099 0.9512 0.8866 0.9797 0.942 0.9563 0.9868 0.9454 0.9145 0.9918 0.9511 0.8795 0.6779 0.9789
Cytoplasmic Foci 0.0156 0.0002 0.0061 0.0066 0.0021 0.0013 0.0088 0.0022 0.0076 0.0011 0.0002 0.0006 0.0087 0.0001 0.0027 0.0258 0.0442 0.0002
Eisosomes 0.0002 0 0 0 0.0002 0 0.0002 0 0.0001 0 0 0 0 0 0.0001 0.0001 0.0003 0
Endoplasmic Reticulum 0.0022 0.0001 0.0006 0.0003 0.0012 0.0007 0.0032 0.0002 0.0011 0.0006 0.0002 0.0006 0.0014 0.0001 0.0009 0.0015 0.0015 0.0002
Endosome 0.0067 0 0.0027 0.0187 0.0068 0.0012 0.0092 0.0001 0.0024 0.0003 0 0.0004 0.0062 0 0.0012 0.0137 0.0174 0.0001
Golgi 0.0025 0 0.0006 0.0178 0.0025 0.0004 0.002 0.0002 0.0008 0 0 0.0001 0.0013 0 0.0008 0.0034 0.0498 0
Lipid Particles 0.0048 0 0.0029 0.0075 0.0012 0 0.0047 0.0005 0.0066 0.0002 0 0.0015 0.0052 0 0.0014 0.0124 0.0468 0
Mitochondria 0.0227 0 0.0137 0.0046 0.0416 0.0012 0.0057 0.001 0.005 0.0021 0 0.0102 0.0026 0 0.0067 0.0023 0.0943 0
None 0.0048 0.0002 0.002 0.0002 0.0036 0.0005 0.0076 0.0008 0.0055 0.0023 0.0002 0.0004 0.0019 0.0001 0.0012 0.0053 0.0064 0.0001
Nuclear Periphery 0.0099 0.0011 0.0064 0.0017 0.0026 0.0049 0.0118 0.0008 0.0031 0.0046 0.0014 0.003 0.0081 0.0008 0.003 0.004 0.0018 0.0017
Nucleolus 0.0021 0 0.0006 0 0.0002 0 0.0018 0.0016 0.0003 0.0023 0 0.0005 0.0037 0 0.0005 0.0032 0.0012 0
Nucleus 0.0408 0.0067 0.0186 0.0076 0.0166 0.0337 0.0336 0.0068 0.0199 0.0226 0.0104 0.0247 0.0352 0.0068 0.0109 0.0167 0.0048 0.0181
Peroxisomes 0.0031 0 0.0055 0.0033 0.0002 0 0.0016 0.0011 0.0015 0.0001 0 0.008 0.0006 0 0.0008 0.0088 0.0288 0
Punctate Nuclear 0.0092 0.0001 0.0018 0.0003 0.0015 0.001 0.0031 0.0002 0.001 0.0006 0.0002 0.0003 0.0029 0 0.0006 0.006 0.0015 0.0001
Vacuole 0.0054 0.0001 0.0028 0.0005 0.0026 0.0006 0.0074 0.0004 0.0008 0.0026 0.0002 0.0024 0.0027 0.0001 0.0024 0.0046 0.0029 0.0002
Vacuole Periphery 0.0023 0 0.0011 0.0004 0.0022 0.0003 0.0037 0.0002 0.0004 0.0019 0 0.0005 0.0014 0 0.0018 0.0015 0.0035 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 99.6588 198.8503 205.2628 156.5125 135.6369 99.0533 133.8723 125.9593 138.6881 125.4628
Translational Efficiency 1.7489 1.775 1.5246 2.2926 2.0227 1.4477 1.8432 2.1452 1.5727 1.919

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1125 467 1884 242 1967 1722 270 1258 3092 2189 2154 1500

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8549.36 10144.38 5008.70 2252.19 8996.21 10265.43 4134.15 3579.13 8833.63 10239.61 4899.08 3365.05
Standard Deviation 2111.61 3891.31 1469.73 688.85 2201.90 2620.89 1260.38 1107.24 2180.11 2938.80 1473.88 1158.87
Intensity Change Log 2 — 0.246792 -0.771380 -1.924488 — 0.190405 -1.121727 -1.329708 — 0.218156 -0.940391 -1.589212

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000058 0.000061 0.000067 0.000661 0.000021 0.000050 0.000584 0.000226 0.000035 0.000052 0.000132 0.000296
Bud Neck 0.000462 0.000722 0.000862 0.002734 0.000199 0.000529 0.006746 0.002004 0.000295 0.000570 0.001600 0.002122
Bud Site 0.000558 0.000575 0.001063 0.004501 0.000280 0.000805 0.003751 0.011135 0.000381 0.000756 0.001400 0.010065
Cell Periphery 0.001055 0.001895 0.000375 0.000663 0.000209 0.000509 0.001111 0.000468 0.000517 0.000804 0.000467 0.000499
Cytoplasm 0.658685* 0.671871* 0.599543* 0.507614* 0.715942* 0.663745* 0.412716* 0.700627* 0.695109* 0.665478* 0.576124* 0.669487*
Cytoplasmic Foci 0.006868 0.016762 0.006541 0.008686 0.001766 0.006163 0.020211 0.009772 0.003622 0.008424 0.008255 0.009597
Eisosomes 0.000001 0.000003 0.000001 0.000017 0.000000 0.000001 0.000010 0.000002 0.000001 0.000001 0.000002 0.000005
Endoplasmic Reticulum 0.000016 0.000011 0.000020 0.002889 0.000013 0.000013 0.000346 0.000059 0.000014 0.000012 0.000061 0.000515
Endosome 0.000021 0.000012 0.000035 0.001986 0.000011 0.000025 0.000366 0.002563 0.000015 0.000023 0.000076 0.002470
Golgi 0.000541 0.000456 0.000165 0.006298 0.000162 0.000365 0.000391 0.002138 0.000300 0.000384 0.000193 0.002810
Lipid Particles 0.000545 0.000379 0.000224 0.001544 0.000096 0.000235 0.001519 0.000788 0.000259 0.000266 0.000386 0.000910
Mitochondria 0.000166 0.000125 0.000249 0.000291 0.000143 0.000183 0.000414 0.000678 0.000151 0.000171 0.000270 0.000615
Mitotic Spindle 0.000461 0.000074 0.001701 0.008812 0.000308 0.001016 0.001023 0.023801 0.000364 0.000815 0.001616 0.021383
None 0.015471 0.019957 0.014203 0.008186 0.021041 0.018151 0.022070 0.011999 0.019014 0.018537 0.015190 0.011384
Nuclear Periphery 0.000160 0.000187 0.000257 0.000981 0.000060 0.000151 0.000690 0.000267 0.000097 0.000159 0.000311 0.000382
Nuclear Periphery Foci 0.000674 0.000684 0.000285 0.007239 0.000259 0.000437 0.001506 0.000941 0.000410 0.000490 0.000438 0.001957
Nucleolus 0.000055 0.000096 0.000090 0.000181 0.000045 0.000074 0.000232 0.000130 0.000048 0.000078 0.000108 0.000138
Nucleus 0.310798* 0.277384* 0.370110* 0.261454* 0.258160* 0.304569* 0.474738* 0.213686* 0.277312* 0.298769* 0.383225* 0.221392*
Peroxisomes 0.000004 0.000003 0.000007 0.000060 0.000003 0.000005 0.000043 0.000107 0.000003 0.000005 0.000011 0.000100
Vacuole 0.003202 0.008512 0.004140 0.165681 0.001219 0.002765 0.051397 0.014834 0.001940 0.003991 0.010063 0.039171
Vacuole Periphery 0.000200 0.000231 0.000062 0.009521 0.000062 0.000210 0.000136 0.003775 0.000112 0.000215 0.000071 0.004702

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.79 -4.21 -7.26 -7.09 -6.69 -8.88 -10.16 -9.87 -7.48 7.85 -5.53 -11.88 -11.36 -9.99 -2.56
Bud Neck -2.13 -9.03 -8.83 -6.62 -5.52 -6.35 -8.72 -11.01 -7.31 7.51 -5.20 -12.77 -13.06 -9.23 3.66
Bud Site -1.37 -6.80 -10.71 -10.23 -8.51 -3.53 -8.93 -7.43 -6.52 2.10 -3.32 -11.99 -8.91 -8.05 -5.14
Cell Periphery -2.57 5.11 2.95 4.72 -2.09 -6.24 -10.81 -4.99 1.33 8.97 -3.46 -2.45 0.72 4.22 3.38
Cytoplasm -0.76 11.53 11.89 11.38 6.37 11.32 32.95 4.41 -5.48 -28.83 8.07 27.23 6.39 -0.21 -15.95
Cytoplasmic Foci -4.84 -2.94 -5.62 0.30 -4.19 -11.08 -9.83 -15.68 -6.83 6.48 -8.90 -12.55 -14.22 -4.77 -0.54
Eisosomes -3.38 -3.26 -11.31 -8.64 -10.74 -6.05 -12.12 -14.88 -11.59 10.13 -4.94 -12.91 -16.00 -12.14 -2.63
Endoplasmic Reticulum 0.86 -7.19 -3.77 -3.78 -3.70 -0.69 -5.57 -4.37 -4.14 5.31 0.63 -7.03 -4.03 -4.05 -2.27
Endosome 1.12 -6.79 -5.61 -5.66 -5.17 -2.62 -7.66 -1.96 -1.89 0.25 -1.88 -10.83 -2.53 -2.49 -1.92
Golgi 1.04 4.45 -0.71 -0.84 -1.12 -1.98 -8.52 -1.44 -0.44 0.83 -0.99 0.34 -1.24 -0.80 -1.30
Lipid Particles 0.55 0.59 -5.76 -6.72 -7.62 -3.62 -10.82 -7.09 -5.76 4.19 -0.53 -5.32 -7.32 -7.34 -4.70
Mitochondria 2.28 -8.51 -0.79 -2.28 4.92 -2.36 -8.50 -0.98 -0.21 3.50 -1.50 -12.57 -0.93 -0.45 2.57
Mitotic Spindle 2.64 -4.68 -2.54 -2.67 -1.50 -1.41 -2.01 -3.97 -3.60 -3.57 -1.33 -4.55 -4.60 -4.29 -2.72
None -0.91 1.90 6.32 5.32 6.99 3.52 8.50 11.75 8.69 -0.46 1.99 7.37 12.58 10.00 5.74
Nuclear Periphery -1.47 -11.61 -7.66 -6.14 -3.27 -9.13 -16.66 -12.41 -4.46 12.88 -6.76 -20.65 -12.82 -6.81 7.10
Nuclear Periphery Foci -0.96 2.11 -5.39 -5.17 -5.76 -4.33 -10.07 -11.05 -7.81 4.58 -2.76 -6.28 -10.40 -8.94 -7.46
Nucleolus -1.95 -11.50 -6.94 -1.91 -1.41 -3.47 -10.68 -7.09 -2.18 8.36 -3.73 -16.47 -8.82 -2.57 5.84
Nucleus 3.58 -9.48 7.18 3.90 13.45 -9.64 -12.02 11.23 19.83 16.87 -5.82 -19.88 15.41 19.57 31.79
Peroxisomes -0.05 -2.48 -9.33 -9.34 -5.91 -1.71 -9.90 -3.97 -3.78 2.64 -1.55 -4.44 -5.14 -4.97 -2.34
Vacuole -4.46 -8.33 -15.31 -14.68 -14.49 -9.16 -12.65 -20.36 -18.88 6.11 -7.87 -15.19 -23.26 -21.98 -14.24
Vacuole Periphery -0.64 1.29 -0.93 -0.83 -1.01 -1.51 -9.25 -1.60 -1.19 -1.17 -1.35 -0.10 -1.78 -1.44 -1.78
Standard name
Human Ortholog
Description Arginase, catabolizes arginine to ornithine and urea; expression responds to both induction by arginine and nitrogen catabolite repression; disruption decreases production of carcinogen ethyl carbamate during wine fermentation and also enhances freeze tolerance
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Car1

Car1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Car1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available