Standard name | |
---|---|
Human Ortholog | |
Description | Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 46 | 39 | 18 | 25 | 21 | 20 | 13 | 13 | 7 | 3 | 1 | 9 | 10 | 6 | 25 | 13 | 21 | 15 | 30 | 38 | 15 | 26 | 23 |
Bud | 1 | 7 | 11 | 4 | 27 | 12 | 21 | 32 | 20 | 23 | 11 | 24 | 4 | 4 | 13 | 1 | 3 | 0 | 12 | 20 | 20 | 20 | 16 | 11 |
Bud Neck | 0 | 0 | 5 | 1 | 3 | 1 | 3 | 2 | 2 | 2 | 3 | 2 | 0 | 0 | 0 | 2 | 3 | 2 | 19 | 48 | 50 | 13 | 9 | 6 |
Bud Site | 5 | 7 | 15 | 2 | 11 | 5 | 10 | 11 | 6 | 8 | 2 | 7 | 0 | 0 | 0 | 4 | 2 | 6 | – | – | – | – | – | – |
Cell Periphery | 1 | 10 | 17 | 11 | 2 | 11 | 10 | 8 | 23 | 21 | 12 | 18 | 13 | 18 | 44 | 25 | 17 | 18 | 2 | 3 | 6 | 1 | 3 | 3 |
Cytoplasm | 52 | 185 | 266 | 211 | 269 | 288 | 273 | 388 | 277 | 250 | 188 | 216 | 175 | 245 | 268 | 118 | 76 | 100 | 58 | 156 | 196 | 69 | 151 | 167 |
Endoplasmic Reticulum | 0 | 18 | 9 | 6 | 4 | 14 | 7 | 5 | 9 | 8 | 1 | 2 | 5 | 2 | 5 | 8 | 4 | 12 | 1 | 4 | 13 | 2 | 18 | 13 |
Endosome | 1 | 3 | 1 | 0 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 6 | 4 | 3 | 4 | 12 | 12 | 1 | 3 | 7 |
Golgi | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 3 | 0 | 2 | 1 |
Mitochondria | 2 | 5 | 11 | 53 | 55 | 114 | 145 | 225 | 198 | 209 | 159 | 242 | 0 | 0 | 3 | 7 | 3 | 7 | 3 | 8 | 53 | 1 | 10 | 7 |
Nucleus | 0 | 1 | 1 | 0 | 0 | 3 | 3 | 4 | 3 | 0 | 4 | 0 | 2 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 1 | 0 | 0 | 5 | 2 | 2 | 0 | 1 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 7 | 8 | 5 | 0 | 11 | 1 | 4 | 3 | 2 | 0 | 1 | 4 | 0 | 1 | 0 | 4 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5 | 4 | 16 | 1 | 10 | 3 | 9 | 5 | 6 | 8 | 0 | 7 | 1 | 0 | 0 | 11 | 9 | 10 | 1 | 4 | 5 | 0 | 0 | 3 |
Vac/Vac Membrane | 2 | 3 | 0 | 0 | 7 | 0 | 12 | 27 | 0 | 4 | 1 | 8 | 0 | 0 | 0 | 21 | 16 | 6 | 2 | 14 | 11 | 4 | 12 | 6 |
Unique Cell Count | 73 | 254 | 342 | 261 | 362 | 396 | 412 | 584 | 436 | 412 | 305 | 409 | 191 | 257 | 286 | 182 | 119 | 146 | 125 | 310 | 419 | 132 | 259 | 258 |
Labelled Cell Count | 90 | 298 | 398 | 308 | 433 | 478 | 521 | 724 | 560 | 540 | 387 | 537 | 211 | 283 | 340 | 236 | 156 | 187 | 125 | 310 | 419 | 132 | 259 | 258 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 6.4 | 4.9 | 4.4 | 4.9 | 4.4 | 4.3 | 4.2 | 3.7 | 3.7 | 3.8 | 3.6 | 6.9 | 6.8 | 5.9 | 8.2 | 9.2 | 9.2 | 7.3 | 7.6 | 7.5 |
Std Deviation (1e-4) | 1.1 | 0.7 | 0.6 | 0.5 | 1.0 | 0.8 | 0.9 | 1.0 | 0.7 | 0.7 | 1.1 | 1.1 | 1.1 | 1.1 | 1.0 | 1.3 | 1.9 | 2.2 | 1.1 | 1.5 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.0 | -0.16 | -0.19 | -0.22 | -0.39 | -0.4 | -0.36 | -0.42 | 0.51 | 0.48 | 0.28 | 0.75 | 0.92 | 0.92 | 0.58 | 0.63 | 0.62 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.6 | -3.3 | -4.5 | 0.8 | -0.1 | 0.9 |
Bud | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.8 | -1.3 | 0.9 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.9 | -3.4 | -3.6 | -1.3 | 0 | -0.1 |
Cell Periphery | 0 | -3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.0 | 4.4 | 3.5 | 3.3 | 2.9 |
Cytoplasm | 0.9 | -1.1 | -1.6 | -3.5 | -3.7 | -4.3 | -5.0 | -4.5 | -7.1 | 4.1 | 6.0 | 5.6 | -3.2 | -3.0 | -2.2 |
Endoplasmic Reticulum | 0 | -1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | 1.1 | 0 | 2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 5.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | 0.4 | 0 | 0.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.6 | -3.5 | -3.7 | 0.7 | 1.2 | 1.0 |
Vacuole | 0 | 0 | 0 | 3.2 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2719 | 3.0495 | 2.7076 | 3.1808 | 2.0267 | 2.8846 | 5.7426 | 5.6257 | 5.6413 | 4.8344 | 6.3073 | 5.8822 | 5.6185 | 5.842 | 5.2749 | 6.079 | 5.4756 | 5.1232 |
Actin | 0.1961 | 0.0204 | 0.0653 | 0.029 | 0.0333 | 0.0353 | 0.0537 | 0.0204 | 0.0512 | 0.0546 | 0.017 | 0.0413 | 0.0391 | 0.004 | 0.0335 | 0.0408 | 0.0242 | 0.0288 |
Bud | 0.002 | 0.0033 | 0.0342 | 0.0106 | 0.0024 | 0.0014 | 0.0071 | 0.0252 | 0.0275 | 0.0381 | 0.0075 | 0.0071 | 0.0063 | 0.0134 | 0.0128 | 0.0089 | 0.0018 | 0.0021 |
Bud Neck | 0.072 | 0.0026 | 0.0087 | 0.0897 | 0.0015 | 0.5346 | 0.0407 | 0.0079 | 0.009 | 0.0009 | 0.0041 | 0.426 | 0.0595 | 0.0065 | 0.0031 | 0.0023 | 0.0015 | 0.4238 |
Bud Periphery | 0.0031 | 0.003 | 0.0515 | 0.0122 | 0.0018 | 0.0023 | 0.0126 | 0.0121 | 0.0453 | 0.0947 | 0.0149 | 0.0052 | 0.0105 | 0.0157 | 0.0111 | 0.0112 | 0.0024 | 0.0027 |
Bud Site | 0.0805 | 0.192 | 0.0417 | 0.0541 | 0.0015 | 0.0027 | 0.0828 | 0.1165 | 0.0452 | 0.0113 | 0.01 | 0.0112 | 0.1113 | 0.2883 | 0.0482 | 0.0168 | 0.0016 | 0.0168 |
Cell Periphery | 0.0049 | 0.005 | 0.0064 | 0.0024 | 0.0007 | 0.0022 | 0.0054 | 0.0037 | 0.0041 | 0.0009 | 0.0041 | 0.0014 | 0.0052 | 0.003 | 0.0018 | 0.0021 | 0.0006 | 0.0018 |
Cytoplasm | 0.3941 | 0.7021 | 0.6857 | 0.6078 | 0.8723 | 0.3668 | 0.4958 | 0.6566 | 0.6445 | 0.5767 | 0.7243 | 0.3801 | 0.5262 | 0.5157 | 0.7362 | 0.7837 | 0.8728 | 0.4602 |
Cytoplasmic Foci | 0.0539 | 0.0115 | 0.0141 | 0.0261 | 0.0447 | 0.0233 | 0.0485 | 0.0135 | 0.0305 | 0.0222 | 0.0888 | 0.0332 | 0.0325 | 0.0145 | 0.047 | 0.0263 | 0.0352 | 0.0273 |
Eisosomes | 0.0026 | 0.0002 | 0.0004 | 0.0011 | 0 | 0.0001 | 0.0009 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0126 | 0.0041 | 0.0089 | 0.0078 | 0.0043 | 0.0022 | 0.0213 | 0.0058 | 0.0089 | 0.0071 | 0.004 | 0.009 | 0.012 | 0.0034 | 0.0039 | 0.0055 | 0.0038 | 0.002 |
Endosome | 0.0422 | 0.009 | 0.0168 | 0.0612 | 0.0079 | 0.0064 | 0.0384 | 0.0078 | 0.0261 | 0.0551 | 0.0231 | 0.0186 | 0.0305 | 0.01 | 0.0199 | 0.0282 | 0.0091 | 0.0087 |
Golgi | 0.0201 | 0.0044 | 0.0083 | 0.005 | 0.0026 | 0.0028 | 0.0125 | 0.0027 | 0.0118 | 0.034 | 0.0054 | 0.0181 | 0.0095 | 0.0012 | 0.0112 | 0.007 | 0.0017 | 0.004 |
Lipid Particles | 0.0118 | 0.0005 | 0.0049 | 0.005 | 0.0007 | 0.0002 | 0.0144 | 0.0011 | 0.0019 | 0.0015 | 0.0009 | 0.0021 | 0.0041 | 0.0003 | 0.0038 | 0.0007 | 0.0006 | 0.0001 |
Mitochondria | 0.0053 | 0.0008 | 0.0058 | 0.04 | 0.0006 | 0.0005 | 0.0041 | 0.0018 | 0.0092 | 0.0175 | 0.0009 | 0.0038 | 0.011 | 0.0006 | 0.0091 | 0.0012 | 0.0005 | 0.0007 |
None | 0.0301 | 0.0367 | 0.0287 | 0.0152 | 0.0178 | 0.0163 | 0.0961 | 0.115 | 0.0596 | 0.0607 | 0.089 | 0.0266 | 0.1124 | 0.1148 | 0.0453 | 0.0302 | 0.0339 | 0.018 |
Nuclear Periphery | 0.0139 | 0.0004 | 0.0028 | 0.0045 | 0.0005 | 0.0001 | 0.0271 | 0.001 | 0.0053 | 0.0017 | 0.0003 | 0.005 | 0.0084 | 0.0008 | 0.0005 | 0.001 | 0.0008 | 0.0001 |
Nucleolus | 0.001 | 0.0001 | 0.0003 | 0.0015 | 0 | 0.0001 | 0.0012 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0011 | 0.0004 | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0001 |
Nucleus | 0.0063 | 0.0009 | 0.0019 | 0.0036 | 0.0006 | 0.0005 | 0.0197 | 0.003 | 0.003 | 0.0018 | 0.0005 | 0.0018 | 0.0019 | 0.002 | 0.0009 | 0.0023 | 0.0008 | 0.0004 |
Peroxisomes | 0.0193 | 0.0003 | 0.0049 | 0.0023 | 0.0017 | 0.0008 | 0.0047 | 0.0007 | 0.0053 | 0.0042 | 0.002 | 0.0037 | 0.0049 | 0.0005 | 0.0062 | 0.0009 | 0.0033 | 0.0008 |
Punctate Nuclear | 0.0241 | 0.0004 | 0.0016 | 0.0014 | 0.0029 | 0.0003 | 0.0068 | 0.0015 | 0.0015 | 0.0009 | 0.0003 | 0.0018 | 0.0082 | 0.0012 | 0.0009 | 0.0006 | 0.0015 | 0.0002 |
Vacuole | 0.0028 | 0.002 | 0.0061 | 0.0157 | 0.0017 | 0.0009 | 0.004 | 0.0027 | 0.007 | 0.0126 | 0.0024 | 0.0016 | 0.0043 | 0.0027 | 0.0031 | 0.028 | 0.0033 | 0.0011 |
Vacuole Periphery | 0.0013 | 0.0002 | 0.0011 | 0.0038 | 0.0002 | 0.0001 | 0.002 | 0.0003 | 0.002 | 0.0031 | 0.0004 | 0.0007 | 0.0014 | 0.0003 | 0.0008 | 0.0016 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.8085 | 11.0665 | 14.4788 | 23.6925 | 24.287 | 15.9542 | 19.8758 | 20.3707 | 20.5923 | 31.0373 |
Translational Efficiency | 0.7533 | 1.1013 | 0.9036 | 0.5674 | 0.6955 | 1.3065 | 0.6419 | 0.6336 | 0.7012 | 0.6482 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
111 | 425 | 1747 | 1193 | 1760 | 909 | 581 | 222 | 1871 | 1334 | 2328 | 1415 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 749.54 | 901.89 | 1123.21 | 912.26 | 813.26 | 758.58 | 999.61 | 912.93 | 809.48 | 804.24 | 1092.36 | 912.37 |
Standard Deviation | 85.94 | 1123.11 | 132.01 | 119.82 | 101.91 | 115.99 | 119.15 | 127.87 | 102.14 | 644.58 | 139.58 | 121.12 |
Intensity Change Log 2 | — | 0.266946 | 0.583550 | 0.283440 | — | -0.100415 | 0.297649 | 0.166788 | — | 0.087458 | 0.441849 | 0.223913 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005885 | 0.010877 | 0.045395 | 0.010819 | 0.019155 | 0.009908 | 0.013920 | 0.002711 | 0.018368 | 0.010217 | 0.037539 | 0.009547 |
Bud Neck | 0.101900 | 0.076128 | 0.025291 | 0.039984 | 0.135749 | 0.090051 | 0.085358 | 0.027742 | 0.133741 | 0.085615 | 0.040282 | 0.038064 |
Bud Site | 0.248376* | 0.235500* | 0.391663* | 0.208424* | 0.285884* | 0.205656* | 0.267076* | 0.079173 | 0.283659* | 0.215164* | 0.360570* | 0.188145* |
Cell Periphery | 0.006435 | 0.000688 | 0.000670 | 0.000370 | 0.003411 | 0.000753 | 0.000509 | 0.000487 | 0.003591 | 0.000732 | 0.000630 | 0.000388 |
Cytoplasm | 0.422189* | 0.354427* | 0.447650* | 0.442019* | 0.362172* | 0.360147* | 0.328022* | 0.295998* | 0.365733* | 0.358324* | 0.417794* | 0.419110* |
Cytoplasmic Foci | 0.116509 | 0.169443 | 0.042015 | 0.038517 | 0.103047 | 0.139466 | 0.061977 | 0.098076 | 0.103846 | 0.149017 | 0.046997 | 0.047862 |
Eisosomes | 0.003320 | 0.000765 | 0.000165 | 0.000139 | 0.002461 | 0.000573 | 0.000214 | 0.000372 | 0.002512 | 0.000634 | 0.000178 | 0.000176 |
Endoplasmic Reticulum | 0.001901 | 0.000466 | 0.000598 | 0.001379 | 0.002501 | 0.000906 | 0.001138 | 0.001146 | 0.002465 | 0.000766 | 0.000733 | 0.001342 |
Endosome | 0.018361 | 0.033755 | 0.001758 | 0.009390 | 0.015100 | 0.039309 | 0.010989 | 0.015321 | 0.015293 | 0.037539 | 0.004062 | 0.010320 |
Golgi | 0.011132 | 0.009733 | 0.001266 | 0.006964 | 0.013850 | 0.016771 | 0.001116 | 0.006848 | 0.013689 | 0.014529 | 0.001228 | 0.006946 |
Lipid Particles | 0.003462 | 0.002586 | 0.000914 | 0.000787 | 0.003016 | 0.002897 | 0.002066 | 0.004527 | 0.003042 | 0.002798 | 0.001202 | 0.001374 |
Mitochondria | 0.006416 | 0.002364 | 0.000193 | 0.006006 | 0.006778 | 0.011713 | 0.000172 | 0.003374 | 0.006756 | 0.008734 | 0.000188 | 0.005594 |
Mitotic Spindle | 0.000419 | 0.001388 | 0.000832 | 0.028767 | 0.000390 | 0.008967 | 0.003401 | 0.022255 | 0.000392 | 0.006553 | 0.001473 | 0.027746 |
None | 0.000089 | 0.000076 | 0.000440 | 0.000840 | 0.000401 | 0.000527 | 0.000546 | 0.000983 | 0.000383 | 0.000383 | 0.000466 | 0.000862 |
Nuclear Periphery | 0.000037 | 0.000029 | 0.000016 | 0.000215 | 0.000024 | 0.000064 | 0.000224 | 0.000930 | 0.000025 | 0.000053 | 0.000068 | 0.000327 |
Nuclear Periphery Foci | 0.000188 | 0.000353 | 0.001022 | 0.001124 | 0.000176 | 0.000275 | 0.002665 | 0.001562 | 0.000177 | 0.000300 | 0.001432 | 0.001193 |
Nucleolus | 0.000056 | 0.000158 | 0.000040 | 0.000312 | 0.000100 | 0.000880 | 0.000155 | 0.001656 | 0.000097 | 0.000650 | 0.000068 | 0.000523 |
Nucleus | 0.003980 | 0.004193 | 0.009921 | 0.032203 | 0.005931 | 0.007911 | 0.015705 | 0.134108* | 0.005815 | 0.006727 | 0.011364 | 0.048191 |
Peroxisomes | 0.007610 | 0.020629 | 0.011606 | 0.008503 | 0.010662 | 0.019127 | 0.017730 | 0.008246 | 0.010481 | 0.019605 | 0.013135 | 0.008462 |
Vacuole | 0.040313 | 0.074661 | 0.018408 | 0.160182 | 0.027340 | 0.080689 | 0.186722 | 0.288232* | 0.028110 | 0.078768 | 0.060414 | 0.180272 |
Vacuole Periphery | 0.001422 | 0.001784 | 0.000138 | 0.003056 | 0.001851 | 0.003407 | 0.000295 | 0.006253 | 0.001825 | 0.002890 | 0.000177 | 0.003558 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.36 | -10.61 | 0.01 | 1.64 | 12.64 | 4.19 | 2.80 | 9.34 | 5.19 | 4.71 | 3.92 | -6.34 | 7.17 | 3.18 | 12.98 |
Bud Neck | 1.46 | 4.61 | 4.83 | 7.69 | 1.62 | 5.87 | 8.35 | 17.35 | 10.49 | 6.75 | 7.08 | 17.64 | 20.54 | 13.65 | 5.69 |
Bud Site | 0.51 | -4.49 | 5.32 | 9.17 | 27.87* | 7.62 | 5.33 | 20.70* | 12.46 | 11.34 | 7.30 | -5.45 | 22.57 | 13.62 | 28.51* |
Cell Periphery | 2.23 | 2.24 | 2.41 | 2.44 | 9.94 | 5.08 | 5.75 | 6.04 | 4.26 | 2.56 | 5.55 | 5.87 | 6.57 | 5.35 | 10.35 |
Cytoplasm | 2.18 | -1.29 | 0.32 | -3.65 | 3.96 | 0.15 | 1.89 | 4.15 | 3.83 | 2.36 | 0.71 | -6.52 | -2.60 | -3.11 | 3.42 |
Cytoplasmic Foci | -2.93 | 4.56 | 5.30 | 15.43 | 4.48 | -5.52 | 7.34 | 5.14 | 8.71 | -0.16 | -7.72 | 14.09 | 17.06 | 22.08 | 4.55 |
Eisosomes | 1.94 | 2.46 | 2.50 | 2.02 | 3.18 | 7.76 | 9.75 | 9.64 | 4.33 | -0.58 | 7.31 | 10.14 | 10.36 | 4.38 | 3.24 |
Endoplasmic Reticulum | 1.20 | 1.07 | 0.47 | -3.61 | -2.90 | 3.14 | 2.41 | 1.67 | -2.88 | -1.36 | 3.57 | 3.53 | 2.15 | -2.84 | -2.82 |
Endosome | -3.66 | 6.18 | 4.99 | 8.88 | -3.63 | -8.36 | 3.62 | 2.71 | 8.99 | 0.37 | -9.58 | 11.56 | 8.57 | 14.54 | -1.88 |
Golgi | 0.76 | 5.77 | 4.34 | 5.14 | -1.69 | -1.53 | 16.74 | 4.48 | 4.81 | -0.87 | -0.52 | 17.38 | 9.89 | 8.04 | -1.93 |
Lipid Particles | 0.52 | 1.94 | 1.80 | 1.37 | -1.43 | 0.19 | 2.04 | -0.36 | -0.43 | -1.81 | 0.41 | 6.05 | 5.41 | 2.54 | -1.47 |
Mitochondria | 1.26 | 1.95 | 1.18 | -0.13 | -2.40 | -2.52 | 8.60 | 3.65 | 4.71 | -1.11 | -1.34 | 8.79 | 3.95 | 4.29 | -2.60 |
Mitotic Spindle | -2.23 | -2.37 | -6.36 | -5.90 | -6.20 | -3.52 | -2.31 | -3.28 | -1.75 | -2.77 | -3.69 | -3.24 | -7.17 | -3.56 | -6.66 |
None | 0.59 | -5.92 | -10.08 | -10.23 | -5.95 | -0.64 | -0.54 | -2.06 | -1.18 | -1.51 | -0.11 | -0.85 | -6.31 | -3.99 | -5.87 |
Nuclear Periphery | 0.30 | 0.73 | -13.19 | -17.08 | -17.63 | -2.39 | -4.58 | -5.62 | -5.41 | -4.36 | -2.37 | -3.98 | -14.71 | -13.59 | -12.82 |
Nuclear Periphery Foci | -1.80 | -6.24 | -10.01 | -6.68 | -0.04 | -1.48 | -7.75 | -5.83 | -5.45 | 1.26 | -2.31 | -9.76 | -12.33 | -9.77 | 1.39 |
Nucleolus | -1.27 | 2.03 | -1.99 | -0.48 | -2.17 | -2.02 | -3.25 | -1.68 | 0.08 | -1.57 | -2.09 | 4.43 | -2.24 | 1.22 | -2.52 |
Nucleus | -0.37 | -8.52 | -20.42 | -20.75 | -17.18 | -1.74 | -10.91 | -7.83 | -7.57 | -6.41 | -1.18 | -11.92 | -20.68 | -19.48 | -17.27 |
Peroxisomes | -3.96 | -1.73 | 1.75 | 5.97 | 6.10 | -4.42 | -2.06 | 3.88 | 6.44 | 4.10 | -5.59 | -1.62 | 6.74 | 9.52 | 7.37 |
Vacuole | -3.43 | 1.84 | -22.85 | -24.06 | -35.06 | -11.92 | -19.85 | -19.47 | -16.59 | -8.08 | -13.80 | -13.20 | -38.82 | -30.27 | -30.41 |
Vacuole Periphery | -0.98 | 5.84 | 0.37 | 0.98 | -2.26 | -3.88 | 9.86 | -0.61 | 0.35 | -1.63 | -3.48 | 11.63 | 0.70 | 2.56 | -2.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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