Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy, Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has novel globular fold essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of higher eukaryote gene Beclin 1; human BECN1 can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 14 | 16 | 4 | – | 2 | 2 | 1 | 2 | 3 | 2 | 2 | 4 |
Bud | 3 | 0 | 5 | 10 | 7 | 24 | 28 | 37 | – | 40 | 30 | 32 | 6 | 6 | 0 | – | 7 | 1 | 1 | 1 | 2 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 9 | 11 | 5 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | – | 2 | 5 | 4 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 15 | 24 | 42 | 57 | 70 | 94 | 118 | – | 125 | 76 | 126 | 74 | 74 | 102 | – | 116 | 36 | 6 | 11 | 5 | 4 | 2 | 4 |
Endoplasmic Reticulum | 4 | 8 | 3 | 2 | 2 | 13 | 0 | 1 | – | 3 | 0 | 2 | 41 | 47 | 13 | – | 17 | 16 | 0 | 0 | 3 | 0 | 1 | 2 |
Endosome | 141 | 18 | 223 | 200 | 254 | 148 | 107 | 150 | – | 16 | 24 | 40 | 368 | 355 | 395 | – | 91 | 116 | 203 | 117 | 262 | 242 | 322 | 383 |
Golgi | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 3 | 5 | 0 | 1 | 1 | 2 | 4 |
Mitochondria | 29 | 59 | 33 | 66 | 34 | 202 | 204 | 251 | – | 392 | 267 | 290 | 2 | 2 | 0 | – | 38 | 25 | 13 | 13 | 9 | 13 | 19 | 17 |
Nucleus | 2 | 3 | 2 | 3 | 3 | 1 | 1 | 4 | – | 0 | 1 | 2 | 13 | 13 | 35 | – | 4 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | – | 2 | 0 | 4 | 0 | 4 | 5 | 1 | 5 |
Nucleolus | 4 | 5 | 11 | 3 | 3 | 8 | 2 | 8 | – | 5 | 2 | 4 | 4 | 4 | 12 | – | 3 | 0 | 0 | 0 | 0 | 2 | 1 | 1 |
Peroxisomes | 0 | 0 | 20 | 23 | 34 | 16 | 25 | 36 | – | 15 | 6 | 11 | 0 | 0 | 0 | – | 3 | 3 | 1 | 0 | 0 | 0 | 1 | 1 |
SpindlePole | 29 | 5 | 67 | 63 | 47 | 61 | 53 | 80 | – | 44 | 34 | 39 | 177 | 162 | 85 | – | 36 | 14 | 5 | 4 | 12 | 7 | 7 | 7 |
Vac/Vac Membrane | 72 | 61 | 44 | 18 | 59 | 39 | 27 | 30 | – | 21 | 12 | 20 | 288 | 281 | 529 | – | 49 | 67 | 28 | 40 | 20 | 36 | 43 | 64 |
Unique Cell Count | 230 | 150 | 303 | 314 | 358 | 444 | 408 | 532 | 522 | 357 | 435 | 778 | 766 | 913 | 278 | 192 | 277 | 198 | 331 | 321 | 415 | 508 | ||
Labelled Cell Count | 307 | 175 | 434 | 430 | 500 | 588 | 542 | 716 | 668 | 458 | 571 | 997 | 972 | 1181 | 370 | 283 | 277 | 198 | 331 | 321 | 415 | 508 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.1 | 5.0 | 4.8 | 5.1 | 4.1 | 4.1 | 4.2 | – | 3.6 | 3.8 | 3.9 | 6.1 | 6.1 | 6.7 | 4.3 | 4.9 | 6.7 | 6.5 | 6.7 | 6.8 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.3 | 1.0 | 1.9 | 1.1 | 1.5 | 1.1 | – | 1.2 | 1.7 | 1.5 | 0.7 | 0.7 | 0.8 | 0.8 | 1.0 | 1.5 | 1.4 | 1.3 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.03 | -0.28 | -0.31 | -0.26 | – | -0.48 | -0.4 | -0.37 | 0.29 | 0.29 | 0.43 | -0.22 | -0.03 | 0.42 | 0.38 | 0.41 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0.3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0.7 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 2.2 | 3.1 | 3.2 | 5.4 | 5.3 | – | 5.8 | 4.8 | 7.0 | 0.8 | 0.9 | 1.6 | – | 9.5 | 3.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.2 | 3.6 | 0 | – | 3.4 | 4.1 |
Endosome | 0 | -0.8 | 0 | 0 | 0 | – | 0 | 0 | 0 | -7.8 | -8.1 | -9.2 | – | -9.9 | -3.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | -0.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | -8.9 | -8.8 | -10.1 | – | 1.0 | 0.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.8 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | -2.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.6 | – | -2.0 | 0 |
Peroxisomes | 0 | 1.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | -7.2 | -7.2 | 0 | – | -3.4 | -2.6 |
SpindlePole | 0 | -3.0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.2 | -0.3 | -5.8 | – | -2.9 | -4.3 |
Vacuole | -3.6 | 0.7 | -2.5 | -3.5 | -4.3 | – | -5.4 | -5.1 | -4.7 | 7.2 | 7.1 | 13.1 | – | 1.0 | 5.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3894 | 1.8354 | 1.7057 | 2.107 | 1.4019 | 1.8975 | 1.7312 | 2.6751 | 2.41 | 1.4692 | 2.2436 | 2.1525 | 1.4133 | 2.2377 | 1.8019 | 2.225 | 1.8839 | 1.7925 |
Actin | 0.0043 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0255 | 0.0005 | 0.0116 | 0.001 | 0.0123 | 0.0028 | 0.0054 | 0 | 0.0059 | 0.0001 | 0.0001 | 0.0001 |
Bud | 0.0002 | 0.0054 | 0.0083 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0008 | 0.0009 | 0.0008 | 0.0002 | 0.0006 | 0.0007 | 0.0007 | 0.0006 | 0.0012 | 0.0017 | 0.0003 | 0.0004 | 0.0008 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0003 |
Bud Periphery | 0.0002 | 0.0015 | 0.0017 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0023 | 0.0105 | 0.01 | 0.0005 | 0.0006 | 0.0005 | 0.0007 | 0.0017 | 0.0046 | 0.0004 | 0.005 | 0.0006 | 0.0022 | 0.0008 | 0.0008 | 0.0001 | 0.0003 | 0.0001 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0157 | 0.049 | 0.0274 | 0.03 | 0.0253 | 0.0454 | 0.0048 | 0.0348 | 0.0284 | 0.0433 | 0.0093 | 0.0107 | 0.0273 | 0.035 | 0.017 | 0.0022 | 0.0242 | 0.0262 |
Cytoplasmic Foci | 0.1513 | 0.1611 | 0.1928 | 0.14 | 0.1643 | 0.1787 | 0.2266 | 0.2051 | 0.1572 | 0.1049 | 0.1673 | 0.209 | 0.1717 | 0.2039 | 0.1472 | 0.1953 | 0.1378 | 0.1786 |
Eisosomes | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0029 | 0.0016 | 0.0009 | 0.001 | 0.0006 | 0.001 | 0.0005 | 0.0011 | 0.0041 | 0.0016 | 0.0066 | 0.0012 | 0.0008 | 0.002 | 0.0019 | 0.0003 | 0.0013 | 0.0009 |
Endosome | 0.6571 | 0.5774 | 0.6067 | 0.6885 | 0.6852 | 0.6008 | 0.452 | 0.5584 | 0.599 | 0.7067 | 0.6364 | 0.6304 | 0.5907 | 0.5846 | 0.658 | 0.6361 | 0.685 | 0.6487 |
Golgi | 0.0224 | 0.0177 | 0.0265 | 0.0252 | 0.0208 | 0.0219 | 0.0642 | 0.0245 | 0.0444 | 0.0393 | 0.035 | 0.0405 | 0.036 | 0.0231 | 0.0291 | 0.027 | 0.0184 | 0.0317 |
Lipid Particles | 0.0531 | 0.0312 | 0.0283 | 0.0101 | 0.0068 | 0.0503 | 0.1158 | 0.0237 | 0.0503 | 0.0108 | 0.0924 | 0.0475 | 0.0759 | 0.06 | 0.0433 | 0.0279 | 0.0242 | 0.0507 |
Mitochondria | 0.0018 | 0.005 | 0.0159 | 0.0046 | 0.0034 | 0.0045 | 0.0028 | 0.0138 | 0.0067 | 0.0036 | 0.001 | 0.0029 | 0.0039 | 0.0008 | 0.0041 | 0.025 | 0.0038 | 0.0027 |
None | 0.0211 | 0.0259 | 0.012 | 0.0108 | 0.0273 | 0.0428 | 0.0043 | 0.0688 | 0.0158 | 0.0112 | 0.0026 | 0.0084 | 0.0443 | 0.0338 | 0.0175 | 0.0001 | 0.0334 | 0.0136 |
Nuclear Periphery | 0.004 | 0.002 | 0.0011 | 0.0053 | 0.0003 | 0.001 | 0.0006 | 0.0007 | 0.0034 | 0.001 | 0.0035 | 0.0007 | 0.0012 | 0.0013 | 0.0026 | 0.0005 | 0.0018 | 0.0032 |
Nucleolus | 0.0009 | 0.0075 | 0.0011 | 0.001 | 0.0004 | 0.0011 | 0.0004 | 0.0004 | 0.0032 | 0.0006 | 0.0003 | 0.0001 | 0.0006 | 0.0004 | 0.0004 | 0.0001 | 0.0005 | 0.0002 |
Nucleus | 0.0029 | 0.0041 | 0.0014 | 0.0073 | 0.0006 | 0.0015 | 0.0004 | 0.0007 | 0.0026 | 0.0007 | 0.001 | 0.0002 | 0.0005 | 0.0005 | 0.0005 | 0.0001 | 0.0004 | 0.0002 |
Peroxisomes | 0.0138 | 0.0098 | 0.0286 | 0.0236 | 0.0185 | 0.0104 | 0.0893 | 0.015 | 0.0248 | 0.0048 | 0.0124 | 0.0225 | 0.0151 | 0.0158 | 0.0217 | 0.0279 | 0.0058 | 0.0148 |
Punctate Nuclear | 0.0033 | 0.0053 | 0.0023 | 0.0013 | 0.0004 | 0.0022 | 0.0054 | 0.0006 | 0.0012 | 0.0006 | 0.0048 | 0.0007 | 0.0052 | 0.0013 | 0.0013 | 0.0004 | 0.0005 | 0.0013 |
Vacuole | 0.0356 | 0.0718 | 0.0287 | 0.0388 | 0.038 | 0.0306 | 0.0047 | 0.0447 | 0.0322 | 0.0575 | 0.0066 | 0.0169 | 0.0154 | 0.0325 | 0.0418 | 0.0467 | 0.0482 | 0.0207 |
Vacuole Periphery | 0.0062 | 0.0115 | 0.0048 | 0.0111 | 0.0065 | 0.0059 | 0.001 | 0.0037 | 0.0073 | 0.0101 | 0.0024 | 0.0038 | 0.0025 | 0.0034 | 0.0063 | 0.0098 | 0.0139 | 0.0057 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.9436 | 7.66 | 4.3724 | 8.9419 | 9.828 | 9.8817 | 7.1493 | 10.0527 | 7.8711 | 11.319 |
Translational Efficiency | 2.0458 | 1.0807 | 1.8691 | 0.9887 | 0.8451 | 0.9321 | 1.1763 | 0.7962 | 1.1635 | 0.7976 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
105 | 203 | 139 | 1478 | 115 | 1920 | 3259 | 1476 | 220 | 2123 | 3398 | 2954 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 982.27 | 742.12 | 1030.42 | 884.76 | 733.50 | 807.13 | 883.79 | 915.94 | 852.23 | 800.91 | 889.79 | 900.34 |
Standard Deviation | 139.05 | 101.74 | 134.61 | 121.19 | 78.64 | 102.61 | 101.69 | 115.85 | 167.03 | 104.30 | 107.25 | 119.57 |
Intensity Change Log 2 | — | -0.404467 | 0.069041 | -0.150833 | — | 0.138004 | 0.268907 | 0.320456 | — | -0.147286 | 0.157893 | 0.069702 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001258 | 0.000345 | 0.001145 | 0.000505 | 0.000079 | 0.000091 | 0.000014 | 0.000351 | 0.000642 | 0.000116 | 0.000061 | 0.000428 |
Bud Neck | 0.014518 | 0.014195 | 0.015368 | 0.017705 | 0.019557 | 0.007701 | 0.015698 | 0.012681 | 0.017152 | 0.008322 | 0.015685 | 0.015195 |
Bud Site | 0.018182 | 0.016108 | 0.027644 | 0.021456 | 0.003907 | 0.004401 | 0.006042 | 0.025178 | 0.010720 | 0.005520 | 0.006925 | 0.023316 |
Cell Periphery | 0.000252 | 0.000053 | 0.000094 | 0.000060 | 0.000168 | 0.000077 | 0.000025 | 0.000064 | 0.000208 | 0.000075 | 0.000028 | 0.000062 |
Cytoplasm | 0.036856 | 0.050966 | 0.013457 | 0.028861 | 0.026683 | 0.009366 | 0.009176 | 0.030349 | 0.031538 | 0.013343 | 0.009351 | 0.029604 |
Cytoplasmic Foci | 0.080614 | 0.214227* | 0.115685 | 0.095827 | 0.129893 | 0.091662 | 0.030217 | 0.106648 | 0.106374 | 0.103382 | 0.033713 | 0.101234 |
Eisosomes | 0.000056 | 0.000046 | 0.000053 | 0.000033 | 0.000097 | 0.000045 | 0.000011 | 0.000017 | 0.000078 | 0.000045 | 0.000012 | 0.000025 |
Endoplasmic Reticulum | 0.006033 | 0.000413 | 0.002124 | 0.001317 | 0.000520 | 0.000846 | 0.000416 | 0.000559 | 0.003152 | 0.000805 | 0.000485 | 0.000938 |
Endosome | 0.224628* | 0.515036* | 0.408589* | 0.415184* | 0.659065* | 0.678289* | 0.493756* | 0.443808* | 0.451720* | 0.662678* | 0.490272* | 0.429486* |
Golgi | 0.026321 | 0.020778 | 0.029384 | 0.016724 | 0.034881 | 0.036879 | 0.005876 | 0.018433 | 0.030796 | 0.035339 | 0.006838 | 0.017578 |
Lipid Particles | 0.004540 | 0.005099 | 0.009840 | 0.005687 | 0.014653 | 0.013554 | 0.001379 | 0.001724 | 0.009826 | 0.012746 | 0.001726 | 0.003706 |
Mitochondria | 0.008224 | 0.009640 | 0.002350 | 0.005375 | 0.008425 | 0.021281 | 0.006114 | 0.007648 | 0.008329 | 0.020168 | 0.005960 | 0.006511 |
Mitotic Spindle | 0.006823 | 0.006358 | 0.076974 | 0.028072 | 0.000773 | 0.001794 | 0.015187 | 0.036008 | 0.003661 | 0.002230 | 0.017715 | 0.032037 |
None | 0.000057 | 0.000168 | 0.000098 | 0.000360 | 0.000090 | 0.000095 | 0.000501 | 0.000081 | 0.000074 | 0.000102 | 0.000484 | 0.000221 |
Nuclear Periphery | 0.002182 | 0.000096 | 0.001236 | 0.001619 | 0.000169 | 0.000238 | 0.000731 | 0.000668 | 0.001129 | 0.000225 | 0.000752 | 0.001144 |
Nuclear Periphery Foci | 0.003008 | 0.000351 | 0.015786 | 0.003676 | 0.000260 | 0.000708 | 0.002038 | 0.001251 | 0.001572 | 0.000674 | 0.002601 | 0.002464 |
Nucleolus | 0.001209 | 0.000501 | 0.006350 | 0.007780 | 0.001080 | 0.000693 | 0.006288 | 0.001815 | 0.001141 | 0.000675 | 0.006291 | 0.004799 |
Nucleus | 0.025169 | 0.003025 | 0.008769 | 0.013190 | 0.003666 | 0.001152 | 0.005007 | 0.002923 | 0.013929 | 0.001331 | 0.005161 | 0.008060 |
Peroxisomes | 0.006095 | 0.020380 | 0.033011 | 0.017198 | 0.024638 | 0.006464 | 0.003467 | 0.016395 | 0.015788 | 0.007795 | 0.004676 | 0.016797 |
Vacuole | 0.514063* | 0.105544 | 0.210307* | 0.301806* | 0.059114 | 0.099216 | 0.364831* | 0.275343* | 0.276249* | 0.099821 | 0.358510* | 0.288583* |
Vacuole Periphery | 0.019911 | 0.016671 | 0.021734 | 0.017564 | 0.012280 | 0.025448 | 0.033224 | 0.018059 | 0.015922 | 0.024609 | 0.032754 | 0.017811 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.06 | -0.19 | 0.84 | -0.93 | 1.51 | -0.44 | 2.76 | -4.39 | -4.31 | -5.58 | 1.32 | 1.43 | 0.41 | -5.10 | -5.70 |
Bud Neck | 0.05 | -0.32 | -0.88 | -1.77 | -0.92 | 2.32 | 0.54 | 1.02 | -4.81 | 1.96 | 2.51 | 0.12 | 0.18 | -6.54 | 0.20 |
Bud Site | 0.60 | -1.18 | -0.40 | -2.09 | 1.25 | -0.46 | -2.52 | -10.37 | -10.60 | -9.94 | 2.11 | 1.51 | -4.17 | -13.21 | -12.61 |
Cell Periphery | 1.68 | 1.22 | 1.62 | -0.62 | 3.22 | 1.96 | 3.13 | 2.21 | 0.78 | -1.79 | 2.18 | 3.02 | 2.44 | 1.07 | -2.97 |
Cytoplasm | -1.20 | 2.31 | 0.08 | 1.72 | -4.00 | 2.50 | 2.35 | -1.25 | -10.73 | -10.45 | 3.44 | 3.94 | -0.83 | -11.38 | -13.69 |
Cytoplasmic Foci | -7.84 | -2.12 | -1.17 | 9.09 | 1.79 | 2.72 | 7.09 | 1.90 | -2.88 | -23.16 | 0.30 | 7.78 | 0.85 | 1.45 | -27.48 |
Eisosomes | 0.72 | -0.38 | 1.71 | 1.80 | 3.04 | 2.30 | 3.87 | 3.57 | 4.78 | -6.15 | 2.34 | 4.97 | 3.91 | 3.50 | -8.52 |
Endoplasmic Reticulum | 2.75 | 1.56 | 2.27 | -5.27 | 2.44 | -0.95 | 0.21 | -0.26 | 1.37 | -2.08 | 2.29 | 2.59 | 2.14 | -0.97 | -4.89 |
Endosome | -9.64 | -5.39 | -6.84 | 6.01 | 0.10 | -0.82 | 6.36 | 8.88 | 27.36 | 7.82 | -9.08 | -1.47 | 2.01 | 33.00 | 11.04 |
Golgi | 0.32 | -0.27 | 0.84 | 1.53 | 2.67 | -0.30 | 4.07 | 2.48 | 8.95 | -7.74 | -0.85 | 3.62 | 2.14 | 10.35 | -9.57 |
Lipid Particles | -0.29 | -1.80 | -0.94 | -0.75 | 1.49 | 0.25 | 3.40 | 3.30 | 8.46 | -1.38 | -1.20 | 3.74 | 2.78 | 6.92 | -5.20 |
Mitochondria | -0.36 | 1.10 | 0.52 | 1.73 | -2.94 | -3.68 | 0.69 | 0.38 | 7.74 | -0.90 | -3.85 | 0.70 | 0.66 | 9.22 | -0.14 |
Mitotic Spindle | 0.06 | -4.22 | -5.41 | -5.61 | 2.77 | -2.01 | -11.16 | -11.77 | -11.44 | -6.29 | 0.82 | -7.20 | -11.63 | -14.69 | -5.83 |
None | -0.96 | -1.74 | -4.12 | -1.68 | -3.45 | -0.04 | -1.70 | -0.25 | -0.36 | 1.68 | -0.68 | -1.80 | -3.66 | -3.16 | 1.00 |
Nuclear Periphery | 1.87 | 0.69 | 0.33 | -3.73 | -0.75 | -0.71 | -7.94 | -4.95 | -4.30 | 0.10 | 1.67 | 0.41 | -0.34 | -4.51 | -1.73 |
Nuclear Periphery Foci | 2.42 | -3.13 | -0.81 | -4.84 | 2.96 | -2.37 | -4.83 | -5.51 | -2.85 | 1.74 | 1.62 | -2.08 | -1.83 | -4.91 | 0.41 |
Nucleolus | 2.03 | -3.74 | -6.13 | -7.02 | -0.91 | 0.50 | -6.78 | -1.80 | -5.06 | 7.32 | 1.17 | -8.84 | -6.12 | -7.92 | 1.65 |
Nucleus | 2.02 | 1.26 | 0.94 | -6.18 | -1.18 | 1.33 | -1.01 | -0.42 | -4.84 | 1.45 | 2.38 | 1.50 | 0.79 | -9.67 | -3.98 |
Peroxisomes | -4.12 | -4.94 | -5.57 | 1.08 | 2.99 | 3.78 | 4.44 | 1.69 | -6.75 | -9.70 | 2.89 | 4.13 | -0.29 | -7.82 | -12.45 |
Vacuole | 12.91 | 8.42 | 6.41 | -17.09 | -5.02 | -3.24 | -24.28 | -17.31 | -24.97 | 8.42 | 8.06 | -3.46 | -1.15 | -34.06 | 7.50 |
Vacuole Periphery | 0.27 | -0.29 | 0.35 | 0.12 | 1.47 | -3.26 | -5.09 | -1.10 | 5.68 | 11.72 | -2.38 | -4.34 | -0.31 | 6.25 | 13.91 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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