Standard name
Human Ortholog
Description Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy, Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has novel globular fold essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of higher eukaryote gene Beclin 1; human BECN1 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0.07 0.07 – 0.08 0.08 0.07 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.1 0.08 0.13 0.16 0.16 0.23 0.22 – 0.24 0.21 0.29 0.1 0.1 0.11 – 0.42 0.19 0 0.06 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 – 0 0 0 0.05 0.06 0 – 0.06 0.08 0 0 0 0 0 0
Endosome 0.61 0.12 0.74 0.64 0.71 0.33 0.26 0.28 – 0 0.07 0.09 0.47 0.46 0.43 – 0.33 0.6 0.73 0.59 0.79 0.76 0.78 0.76
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.39 0.11 0.21 0.09 0.45 0.5 0.47 – 0.75 0.75 0.67 0 0 0 – 0.14 0.13 0.05 0.07 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.07 0.09 0 0.06 0.07 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0.13 0 0.22 0.2 0.13 0.14 0.13 0.15 – 0.08 0.1 0.09 0.23 0.21 0.09 – 0.13 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.31 0.41 0.15 0.06 0.16 0.09 0.07 0.06 – 0 0 0.05 0.37 0.37 0.58 – 0.18 0.35 0.1 0.2 0.06 0.11 0.11 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 1 0 – 0 0 0 14 16 4 – 2 2 1 2 3 2 2 4
Bud 3 0 5 10 7 24 28 37 – 40 30 32 6 6 0 – 7 1 1 1 2 1 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 1 0 0 9 11 5 – 1 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 3 0 1 – 2 5 4 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 3 0 1 1 1 0 – 0 0 0 0 0 0 0 0
Cytoplasm 20 15 24 42 57 70 94 118 – 125 76 126 74 74 102 – 116 36 6 11 5 4 2 4
Endoplasmic Reticulum 4 8 3 2 2 13 0 1 – 3 0 2 41 47 13 – 17 16 0 0 3 0 1 2
Endosome 141 18 223 200 254 148 107 150 – 16 24 40 368 355 395 – 91 116 203 117 262 242 322 383
Golgi 2 0 0 0 0 1 0 0 – 0 0 0 0 0 1 – 1 3 5 0 1 1 2 4
Mitochondria 29 59 33 66 34 202 204 251 – 392 267 290 2 2 0 – 38 25 13 13 9 13 19 17
Nucleus 2 3 2 3 3 1 1 4 – 0 1 2 13 13 35 – 4 0 1 1 0 1 0 1
Nuclear Periphery 1 1 1 0 0 1 0 0 – 1 1 0 0 0 0 – 2 0 4 0 4 5 1 5
Nucleolus 4 5 11 3 3 8 2 8 – 5 2 4 4 4 12 – 3 0 0 0 0 2 1 1
Peroxisomes 0 0 20 23 34 16 25 36 – 15 6 11 0 0 0 – 3 3 1 0 0 0 1 1
SpindlePole 29 5 67 63 47 61 53 80 – 44 34 39 177 162 85 – 36 14 5 4 12 7 7 7
Vac/Vac Membrane 72 61 44 18 59 39 27 30 – 21 12 20 288 281 529 – 49 67 28 40 20 36 43 64
Unique Cell Count 230 150 303 314 358 444 408 532 522 357 435 778 766 913 278 192 277 198 331 321 415 508
Labelled Cell Count 307 175 434 430 500 588 542 716 668 458 571 997 972 1181 370 283 277 198 331 321 415 508


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.1 5.0 4.8 5.1 4.1 4.1 4.2 – 3.6 3.8 3.9 6.1 6.1 6.7 4.3 4.9 6.7 6.5 6.7 6.8
Std Deviation (1e-4) 0.7 0.9 1.3 1.0 1.9 1.1 1.5 1.1 – 1.2 1.7 1.5 0.7 0.7 0.8 0.8 1.0 1.5 1.4 1.3 1.2
Intensity Change (Log2) – – – -0.07 0.03 -0.28 -0.31 -0.26 – -0.48 -0.4 -0.37 0.29 0.29 0.43 -0.22 -0.03 0.42 0.38 0.41 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud 0 0.3 0 0 0 – 0 0 0 0 0 0 – 0.7 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cytoplasm 2.2 3.1 3.2 5.4 5.3 – 5.8 4.8 7.0 0.8 0.9 1.6 – 9.5 3.6
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 3.2 3.6 0 – 3.4 4.1
Endosome 0 -0.8 0 0 0 – 0 0 0 -7.8 -8.1 -9.2 – -9.9 -3.1
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Mitochondria 0 -0.6 0 0 0 – 0 0 0 -8.9 -8.8 -10.1 – 1.0 0.7
Nucleus 0 0 0 0 0 – 0 0 0 0 0 2.8 – 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Nucleolus 0 -2.5 0 0 0 – 0 0 0 0 0 -2.6 – -2.0 0
Peroxisomes 0 1.4 0 0 0 – 0 0 0 -7.2 -7.2 0 – -3.4 -2.6
SpindlePole 0 -3.0 0 0 0 – 0 0 0 0.2 -0.3 -5.8 – -2.9 -4.3
Vacuole -3.6 0.7 -2.5 -3.5 -4.3 – -5.4 -5.1 -4.7 7.2 7.1 13.1 – 1.0 5.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3894 1.8354 1.7057 2.107 1.4019 1.8975 1.7312 2.6751 2.41 1.4692 2.2436 2.1525 1.4133 2.2377 1.8019 2.225 1.8839 1.7925
Actin 0.0043 0.0002 0.0006 0.0001 0.0001 0.0001 0.0255 0.0005 0.0116 0.001 0.0123 0.0028 0.0054 0 0.0059 0.0001 0.0001 0.0001
Bud 0.0002 0.0054 0.0083 0.0003 0.0002 0.0004 0.0001 0.0007 0.0007 0.0001 0.0004 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0001
Bud Neck 0.0008 0.0009 0.0008 0.0002 0.0006 0.0007 0.0007 0.0006 0.0012 0.0017 0.0003 0.0004 0.0008 0.0003 0.0004 0.0002 0.0003 0.0003
Bud Periphery 0.0002 0.0015 0.0017 0.0003 0.0002 0.0003 0.0001 0.0003 0.0006 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0001
Bud Site 0.0023 0.0105 0.01 0.0005 0.0006 0.0005 0.0007 0.0017 0.0046 0.0004 0.005 0.0006 0.0022 0.0008 0.0008 0.0001 0.0003 0.0001
Cell Periphery 0.0001 0.0003 0.0001 0 0.0001 0.0002 0 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.0157 0.049 0.0274 0.03 0.0253 0.0454 0.0048 0.0348 0.0284 0.0433 0.0093 0.0107 0.0273 0.035 0.017 0.0022 0.0242 0.0262
Cytoplasmic Foci 0.1513 0.1611 0.1928 0.14 0.1643 0.1787 0.2266 0.2051 0.1572 0.1049 0.1673 0.209 0.1717 0.2039 0.1472 0.1953 0.1378 0.1786
Eisosomes 0.0001 0.0001 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0029 0.0016 0.0009 0.001 0.0006 0.001 0.0005 0.0011 0.0041 0.0016 0.0066 0.0012 0.0008 0.002 0.0019 0.0003 0.0013 0.0009
Endosome 0.6571 0.5774 0.6067 0.6885 0.6852 0.6008 0.452 0.5584 0.599 0.7067 0.6364 0.6304 0.5907 0.5846 0.658 0.6361 0.685 0.6487
Golgi 0.0224 0.0177 0.0265 0.0252 0.0208 0.0219 0.0642 0.0245 0.0444 0.0393 0.035 0.0405 0.036 0.0231 0.0291 0.027 0.0184 0.0317
Lipid Particles 0.0531 0.0312 0.0283 0.0101 0.0068 0.0503 0.1158 0.0237 0.0503 0.0108 0.0924 0.0475 0.0759 0.06 0.0433 0.0279 0.0242 0.0507
Mitochondria 0.0018 0.005 0.0159 0.0046 0.0034 0.0045 0.0028 0.0138 0.0067 0.0036 0.001 0.0029 0.0039 0.0008 0.0041 0.025 0.0038 0.0027
None 0.0211 0.0259 0.012 0.0108 0.0273 0.0428 0.0043 0.0688 0.0158 0.0112 0.0026 0.0084 0.0443 0.0338 0.0175 0.0001 0.0334 0.0136
Nuclear Periphery 0.004 0.002 0.0011 0.0053 0.0003 0.001 0.0006 0.0007 0.0034 0.001 0.0035 0.0007 0.0012 0.0013 0.0026 0.0005 0.0018 0.0032
Nucleolus 0.0009 0.0075 0.0011 0.001 0.0004 0.0011 0.0004 0.0004 0.0032 0.0006 0.0003 0.0001 0.0006 0.0004 0.0004 0.0001 0.0005 0.0002
Nucleus 0.0029 0.0041 0.0014 0.0073 0.0006 0.0015 0.0004 0.0007 0.0026 0.0007 0.001 0.0002 0.0005 0.0005 0.0005 0.0001 0.0004 0.0002
Peroxisomes 0.0138 0.0098 0.0286 0.0236 0.0185 0.0104 0.0893 0.015 0.0248 0.0048 0.0124 0.0225 0.0151 0.0158 0.0217 0.0279 0.0058 0.0148
Punctate Nuclear 0.0033 0.0053 0.0023 0.0013 0.0004 0.0022 0.0054 0.0006 0.0012 0.0006 0.0048 0.0007 0.0052 0.0013 0.0013 0.0004 0.0005 0.0013
Vacuole 0.0356 0.0718 0.0287 0.0388 0.038 0.0306 0.0047 0.0447 0.0322 0.0575 0.0066 0.0169 0.0154 0.0325 0.0418 0.0467 0.0482 0.0207
Vacuole Periphery 0.0062 0.0115 0.0048 0.0111 0.0065 0.0059 0.001 0.0037 0.0073 0.0101 0.0024 0.0038 0.0025 0.0034 0.0063 0.0098 0.0139 0.0057

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.9436 7.66 4.3724 8.9419 9.828 9.8817 7.1493 10.0527 7.8711 11.319
Translational Efficiency 2.0458 1.0807 1.8691 0.9887 0.8451 0.9321 1.1763 0.7962 1.1635 0.7976

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy, Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has novel globular fold essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of higher eukaryote gene Beclin 1; human BECN1 can complement yeast null mutant
Localization
Cell Percentages endosome (24%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps30

Vps30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available