Standard name
Human Ortholog
Description Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 – 0 0 0 0 0.07 0.12 0.2 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0 0 – 0.07 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.52 0.68 0.73 0.75 0.7 0.65 0.61 – 0.53 0.51 0.52 0.54 0.78 0.61 0.47 0.59 0.55 0.6 0.4 0.5 0.45 0.5 0.54 0.54
Nuclear Periphery 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleolus 0.48 0.35 0.23 0.21 0.28 0.36 0.4 – 0.53 0.56 0.56 0.55 0.2 0.33 0.29 0.33 0.4 0.36 0.42 0.36 0.43 0.25 0.31 0.33
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0.1 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 1 0 0 0 0 0 2 – 2 1 1 0 0 0 0 0 1 1 2 0 3 1 1 0
Bud Neck 0 0 0 2 0 0 1 – 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3 1 – 2 0 8 3 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 4 6 10 8 5 – 5 3 6 5 8 14 35 9 7 7 0 2 0 0 3 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 2 2 3 0 0 0 0 0 0
Endosome 2 3 2 0 3 0 3 – 0 0 0 0 1 2 3 4 0 4 7 2 3 1 1 2
Golgi 0 0 0 0 1 0 1 – 0 0 0 0 0 1 0 0 2 2 0 0 0 0 0 0
Mitochondria 14 3 0 1 2 5 8 – 15 12 24 8 0 0 0 6 8 3 3 1 1 0 0 3
Nucleus 104 81 92 141 143 177 194 – 121 130 160 182 90 73 83 117 105 134 86 71 77 20 61 65
Nuclear Periphery 10 0 1 6 5 9 9 – 8 8 7 6 2 5 5 7 15 3 7 0 0 0 0 0
Nucleolus 96 42 29 39 58 100 126 – 121 142 174 183 23 39 51 66 76 81 92 52 73 10 34 39
Peroxisomes 0 0 0 0 1 0 0 – 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0
SpindlePole 1 2 7 0 5 2 0 – 0 3 0 3 1 3 7 2 2 6 7 2 5 0 0 0
Vac/Vac Membrane 5 2 1 3 1 7 2 – 2 2 4 2 4 0 2 9 7 5 6 7 4 3 8 4
Unique Cell Count 200 120 126 188 205 274 317 229 254 310 335 116 119 176 198 192 225 218 143 172 41 113 120
Labelled Cell Count 236 136 136 198 229 311 353 278 301 387 394 129 137 188 222 225 251 218 143 172 41 113 120


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 8.7 8.5 9.1 9.2 8.5 8.4 – 7.8 8.0 7.4 8.1 9.3 10.2 9.1 12.4 12.1 10.9 – 8.8 9.9
Std Deviation (1e-4) 1.6 2.1 1.5 2.2 2.0 2.0 2.1 – 2.5 1.9 2.1 2.3 2.5 2.3 2.4 6.4 3.6 2.9 – 1.5 2.3
Intensity Change (Log2) – – – 0.09 0.11 -0.0 -0.02 – -0.13 -0.08 -0.2 -0.08 0.13 0.27 0.09 0.54 0.51 0.36 – 0.05 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.7 0 0 – 0 0 0 0 1.3 2.6 4.3 0.6 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 2.9 0 3.2 0 0 0 0 0 0 0
Nucleus 0.4 -0.6 -1.7 -2.3 – -3.7 -4.1 -4.1 -3.6 0.8 -1.9 -4.5 -2.6 -3.3 -2.5
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 2.8 0
Nucleolus -0.5 1.1 2.7 3.3 – 5.4 6.1 6.3 6.1 -0.6 1.7 1.2 2.0 3.1 2.5
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 -0.6 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5773 5.2249 4.8172 4.1614 4.2987 4.6617 5.5428 4.5564 4.8557 4.0821 4.3119 4.2926 5.1136 4.3857 4.2345 4.3968 3.7691 4.0725
Actin 0.0088 0 0.0067 0.0001 0.0012 0.0002 0.0269 0.0002 0.0036 0.0001 0.0006 0.0163 0.0295 0.0009 0.009 0 0.0034 0.0031
Bud 0.0002 0 0.0003 0.0002 0.0019 0.0001 0.0004 0.0001 0.0001 0 0.0006 0.0001 0.0004 0.0001 0.0001 0 0.0001 0.0001
Bud Neck 0.0007 0.0002 0.0007 0.0002 0.0008 0.0012 0.0007 0.0002 0.0004 0.0002 0.0014 0.0033 0.001 0.0002 0.0003 0.0001 0.0004 0.0011
Bud Periphery 0.0005 0 0.0007 0.0004 0.0078 0.0002 0.0006 0.0002 0.0003 0 0.0018 0.0004 0.0008 0.0002 0.0002 0.0001 0.0003 0.0004
Bud Site 0.0005 0.0001 0.0032 0.0003 0.0009 0.0002 0.0025 0.0001 0.0005 0 0.0005 0.0003 0.0039 0.0003 0.0003 0 0.0001 0.0005
Cell Periphery 0.0003 0 0.0001 0.0002 0.0003 0.0001 0.0002 0 0.0001 0 0.0005 0.0001 0.0004 0 0 0 0 0.0002
Cytoplasm 0.0012 0 0.0004 0.0003 0.0002 0 0.01 0 0.0006 0 0.0013 0.0002 0.0033 0.0001 0.0001 0 0.0001 0.0004
Cytoplasmic Foci 0.002 0 0.0034 0.0003 0.0003 0.0004 0.0255 0 0.0082 0 0.0009 0.005 0.0211 0.0027 0 0 0.0006 0.0028
Eisosomes 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0008 0 0.0004 0.0001 0.0002 0.0001 0.0012 0 0.0004 0 0.0008 0.0003 0.0029 0 0 0 0.0008 0.0003
Endosome 0.0032 0 0.0027 0.0003 0.0035 0.0007 0.0245 0 0.0048 0 0.0039 0.0034 0.0189 0.0021 0 0 0.0113 0.0038
Golgi 0.001 0 0.0014 0 0.001 0.0003 0.0085 0 0.0064 0 0.0003 0.0048 0.0087 0.0005 0.0001 0 0.0061 0.001
Lipid Particles 0.0042 0 0.0065 0.0005 0.002 0.0026 0.0299 0.0001 0.0136 0 0.0031 0.0171 0.0398 0.0015 0 0 0.0037 0.0045
Mitochondria 0.0048 0.0001 0.005 0.0014 0.0176 0.0011 0.0057 0.0008 0.0067 0.0002 0.0036 0.0087 0.012 0.0007 0.0008 0.0002 0.0112 0.0072
None 0.002 0 0.0019 0.0003 0.0004 0.0001 0.0107 0.0001 0.0017 0 0.0006 0.0004 0.0025 0.0001 0 0 0.0003 0.0003
Nuclear Periphery 0.0241 0.0075 0.0623 0.0095 0.0955 0.0035 0.0323 0.0266 0.0772 0.0538 0.0803 0.0123 0.0149 0.0425 0.0425 0.0035 0.0076 0.0111
Nucleolus 0.3098 0.135 0.1675 0.2075 0.4085 0.3955 0.1575 0.1673 0.16 0.1486 0.4259 0.3166 0.1386 0.1308 0.134 0.1099 0.4167 0.3397
Nucleus 0.6247 0.8268 0.7012 0.7421 0.4184 0.5859 0.635 0.7856 0.67 0.7871 0.4286 0.5835 0.6636 0.8034 0.801 0.8826 0.4845 0.6148
Peroxisomes 0.0004 0 0.0025 0.0001 0.0004 0.0009 0.0129 0 0.014 0 0.0003 0.0034 0.01 0.0017 0.0001 0 0.0004 0.0005
Punctate Nuclear 0.0082 0.0301 0.0316 0.034 0.032 0.0063 0.009 0.018 0.0292 0.0094 0.0215 0.0212 0.0216 0.0113 0.0106 0.0034 0.05 0.003
Vacuole 0.001 0 0.0004 0.0012 0.0036 0.0003 0.0036 0.0001 0.0005 0.0001 0.0159 0.0012 0.004 0.0002 0.0001 0 0.0005 0.0042
Vacuole Periphery 0.0013 0.0001 0.0009 0.001 0.0035 0.0003 0.0024 0.0004 0.0017 0.0003 0.0074 0.0015 0.0017 0.0007 0.0005 0.0001 0.0015 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.4983 20.44 20.3676 16.9327 22.8695 26.77 25.2431 17.137 14.5623 24.4167
Translational Efficiency 1.1388 1.4555 0.8294 0.9364 1.0361 0.8535 1.0048 1.0659 1.0818 0.8748

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1145 977 1330 127 1676 1918 194 92 2821 2895 1524 219

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 658.55 866.59 1174.21 1113.57 869.70 927.13 1215.69 1098.08 784.00 906.70 1179.49 1107.06
Standard Deviation 97.44 186.30 194.27 154.53 141.75 149.31 153.75 158.91 162.92 165.23 190.10 156.57
Intensity Change Log 2 — 0.396057 0.834326 0.757827 — 0.092254 0.483186 0.336393 — 0.231074 0.645070 0.533243

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000520 0.001197 0.001150 0.001442 0.000485 0.000554 0.002149 0.002026 0.000499 0.000771 0.001277 0.001687
Bud Neck 0.002178 0.002682 0.009535 0.005684 0.001835 0.004260 0.006117 0.004430 0.001974 0.003727 0.009100 0.005157
Bud Site 0.000758 0.001663 0.001711 0.001735 0.000741 0.000608 0.001962 0.001366 0.000748 0.000964 0.001743 0.001580
Cell Periphery 0.000072 0.000099 0.000052 0.000042 0.000047 0.000032 0.000131 0.000064 0.000057 0.000055 0.000062 0.000051
Cytoplasm 0.002594 0.003668 0.000387 0.000120 0.000701 0.000728 0.000518 0.000267 0.001470 0.001720 0.000404 0.000182
Cytoplasmic Foci 0.000911 0.001700 0.000109 0.000168 0.000336 0.000772 0.000337 0.000240 0.000570 0.001085 0.000138 0.000198
Eisosomes 0.000036 0.000043 0.000048 0.000056 0.000030 0.000039 0.000074 0.000062 0.000033 0.000040 0.000051 0.000059
Endoplasmic Reticulum 0.001964 0.003005 0.001347 0.003519 0.001463 0.001583 0.004324 0.004528 0.001666 0.002063 0.001726 0.003943
Endosome 0.000801 0.000820 0.000151 0.000213 0.000250 0.001070 0.000704 0.000272 0.000474 0.000986 0.000222 0.000238
Golgi 0.001189 0.001807 0.000460 0.000624 0.000607 0.001010 0.003271 0.000750 0.000843 0.001279 0.000818 0.000677
Lipid Particles 0.000577 0.000559 0.000082 0.000223 0.000789 0.000520 0.000283 0.000331 0.000703 0.000533 0.000107 0.000268
Mitochondria 0.003018 0.001990 0.002275 0.001511 0.001036 0.003035 0.002052 0.001403 0.001840 0.002682 0.002247 0.001465
Mitotic Spindle 0.028497 0.015787 0.040625 0.090362 0.016480 0.018320 0.090088 0.053040 0.021357 0.017465 0.046921 0.074684
None 0.017241 0.008511 0.001875 0.001173 0.004371 0.001289 0.005088 0.002289 0.009595 0.003726 0.002284 0.001642
Nuclear Periphery 0.002489 0.000633 0.000612 0.001209 0.000520 0.000912 0.000837 0.000784 0.001319 0.000818 0.000641 0.001030
Nuclear Periphery Foci 0.049358 0.012873 0.001438 0.008783 0.009434 0.004298 0.006486 0.018696 0.025638 0.007192 0.002080 0.012948
Nucleolus 0.419721* 0.381184* 0.444341* 0.407364* 0.434343* 0.352380* 0.345223* 0.358271* 0.428408* 0.362101* 0.431724* 0.386740*
Nucleus 0.465518* 0.556565* 0.486137* 0.467121* 0.524656* 0.604777* 0.515631* 0.540329* 0.500653* 0.588506* 0.489892* 0.497875*
Peroxisomes 0.001194 0.003803 0.006703 0.006847 0.001522 0.001817 0.008552 0.009183 0.001389 0.002487 0.006939 0.007828
Vacuole 0.000846 0.001054 0.000625 0.001422 0.000194 0.000789 0.004342 0.001320 0.000459 0.000878 0.001098 0.001379
Vacuole Periphery 0.000516 0.000356 0.000337 0.000382 0.000158 0.001208 0.001832 0.000348 0.000304 0.000920 0.000528 0.000368

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.86 -11.38 -6.44 -1.70 -0.88 -1.22 -5.48 -7.68 -7.41 -0.72 -4.49 -14.11 -9.84 -7.77 -2.98
Bud Neck -1.78 -15.87 -2.84 -2.40 4.14 -5.85 -7.84 -5.19 -2.57 1.05 -5.99 -17.98 -4.94 -2.89 5.14
Bud Site -1.72 -3.91 -1.51 -0.11 1.12 0.67 -4.02 -3.05 -3.69 -0.39 -0.74 -4.83 -2.95 -2.62 0.75
Cell Periphery -1.56 1.28 2.53 3.39 1.38 1.68 -1.42 -2.22 -4.75 0.64 0.60 -0.66 -0.28 -1.02 0.47
Cytoplasm -0.94 3.01 3.46 3.35 3.03 -0.17 1.34 1.43 0.90 -0.07 -0.61 3.30 3.93 3.40 1.78
Cytoplasmic Foci -1.16 3.47 3.35 2.87 -0.65 -1.36 0.88 1.05 2.12 0.11 -1.57 3.86 3.44 3.66 -1.31
Eisosomes -1.88 -6.14 -3.24 -2.42 -0.86 -3.78 -9.45 -5.67 -4.67 0.11 -3.81 -12.59 -6.58 -5.25 -1.95
Endoplasmic Reticulum -4.19 1.07 -3.44 -1.21 -3.83 -0.60 -5.63 -5.82 -5.71 -1.44 -3.08 -1.96 -6.72 -5.74 -5.49
Endosome -0.26 3.08 2.63 2.57 -1.06 -2.21 -1.10 -1.44 1.75 0.52 -1.98 2.37 1.43 2.63 -1.31
Golgi -0.78 1.72 1.34 1.81 -2.19 -1.43 -1.02 -1.62 0.51 0.86 -1.28 0.41 0.56 1.91 -0.03
Lipid Particles 0.39 5.91 3.45 2.16 -2.45 0.61 1.18 0.80 0.11 -1.26 0.67 2.41 1.52 0.85 -3.70
Mitochondria 2.36 1.29 3.41 1.62 3.45 -2.90 -2.08 -2.64 1.67 0.73 -1.73 -2.86 0.93 2.18 3.60
Mitotic Spindle 3.15 -4.59 -3.47 -4.15 -2.27 -0.25 -4.58 -2.58 -2.54 1.00 1.94 -8.15 -4.48 -4.84 -1.83
None 4.01 8.24 8.67 6.37 1.53 3.78 0.47 2.35 -2.37 0.96 5.97 7.88 8.66 4.72 0.98
Nuclear Periphery 7.73 7.60 3.04 -2.05 -1.68 -2.19 -2.53 -2.76 -0.39 -0.62 3.34 4.79 0.62 -1.62 -1.96
Nuclear Periphery Foci 8.78 12.56 8.63 0.78 -3.42 4.02 1.28 -0.78 -1.62 -1.19 9.95 13.27 3.94 -1.61 -3.21
Nucleolus 4.79 -1.78 1.02 -1.13 1.83 11.99 3.82 4.84 1.04 1.38 12.48 -0.13 3.85 -0.72 3.74
Nucleus -10.00 -1.53 -0.10 4.54 0.61 -11.30 1.28 -1.09 2.13 -1.69 -15.59 2.80 -0.05 5.53 -1.31
Peroxisomes -7.74 -15.59 -6.67 -3.45 0.61 -0.60 -7.75 -5.73 -5.64 -0.38 -4.56 -16.32 -8.55 -7.20 -0.33
Vacuole -0.72 -0.52 -1.10 -0.55 -0.88 -2.99 -1.76 -1.79 -1.56 -0.39 -1.92 -2.57 -2.18 -1.76 -1.35
Vacuole Periphery 0.73 0.66 0.53 -0.25 -0.11 -3.64 -1.22 -3.28 2.50 0.89 -3.09 -1.46 -1.31 2.50 0.56
Standard name
Human Ortholog
Description Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
Localization
Cell Percentages nucleus (50%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tbf1

Tbf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tbf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available