Standard name
Human Ortholog
Description Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.98 0.99 0.98 0.97 0.96 – 0.83 0.8 0.8 0.85 0.98 1.0 0.99 0.96 0.96 0.96 0.75 0.84 0.8 0.61 0.76 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.05 0.05 0.05 0 0 0.05 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.09 – 0.24 0.24 0.31 0.15 0 0 0 0 0 0 0 0 0.06 0.08 0.06 0.08
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 1 0 2 0 – 2 2 0 0 0 0 0 1 1 1 12 10 6 4 5 14
Bud 3 3 1 2 1 2 1 – 4 5 7 4 7 0 0 0 0 0 16 8 5 7 13 15
Bud Neck 0 0 4 6 10 13 7 – 6 8 11 13 0 0 0 1 1 1 12 7 4 6 6 11
Bud Site 0 0 0 0 0 0 0 – 0 1 1 1 1 0 0 0 0 0 – – – – – –
Cell Periphery 10 18 7 5 9 15 10 – 10 7 5 3 7 5 3 23 20 19 7 5 8 7 8 6
Cytoplasm 470 435 315 230 331 403 453 – 216 224 314 301 359 411 497 355 267 241 386 398 251 112 272 237
Endoplasmic Reticulum 16 2 0 0 0 1 0 – 0 3 0 0 1 0 2 26 15 12 26 3 3 9 8 2
Endosome 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 2 1 13 4 0 4 3 2
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 2 0 1 2 0 0 0 2 2
Mitochondria 9 6 0 0 7 12 41 – 61 66 123 54 4 2 2 5 1 1 10 13 17 15 21 26
Nucleus 1 0 0 0 3 1 1 – 1 3 3 3 2 1 0 6 3 1 0 0 0 0 0 0
Nuclear Periphery 2 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 1 0 0 1 0 0 1 1 0
SpindlePole 1 1 0 0 0 1 3 – 1 6 7 17 1 0 0 1 0 0 4 1 1 0 2 1
Vac/Vac Membrane 2 4 3 0 1 1 2 – 0 0 0 1 0 0 0 4 3 1 13 12 5 10 8 16
Unique Cell Count 494 448 321 232 337 415 471 259 280 394 355 368 413 501 369 279 251 518 473 314 184 361 342
Labelled Cell Count 516 469 332 244 362 451 518 301 326 472 397 385 420 505 427 313 279 518 473 314 184 361 342


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.3 5.4 4.5 5.0 4.0 4.0 – 3.5 3.2 3.1 3.6 4.4 4.4 4.3 6.5 6.9 6.4 4.9 4.8 4.8
Std Deviation (1e-4) 0.9 0.9 1.2 1.1 1.1 0.8 1.2 – 0.8 0.7 1.1 0.9 1.2 1.0 1.2 1.0 1.3 1.2 1.7 1.9 1.6
Intensity Change (Log2) – – – -0.27 -0.11 -0.44 -0.45 – -0.63 -0.77 -0.81 -0.6 -0.3 -0.29 -0.33 0.27 0.35 0.25 -0.14 -0.17 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8568 2.3593 2.0537 1.7802 3.0293 2.5882 -0.6333 -0.133 -0.3663 0.1439 -0.2503 0.1662 -0.5653 0.053 -0.5002 -0.2536 1.0135 0.2569
Actin 0.0308 0.0034 0.0072 0.0085 0.0295 0.0089 0.0151 0.0044 0.0146 0.0203 0.0124 0.0069 0.0427 0.0018 0.0322 0.0011 0.0509 0.0125
Bud 0.0008 0.0003 0.0001 0.0002 0.0012 0.0004 0.0024 0.0038 0.0014 0.0004 0.0003 0.0005 0.0016 0.0033 0.006 0.0152 0.0037 0.0043
Bud Neck 0.0109 0.0004 0.0002 0.0002 0.0007 0.0063 0.0112 0.0013 0.0011 0.0037 0.0012 0.0038 0.0023 0.0004 0.0018 0.0013 0.0061 0.0066
Bud Periphery 0.0023 0.0006 0.0001 0.0005 0.0026 0.0013 0.0037 0.0056 0.0021 0.0022 0.0005 0.0013 0.0053 0.0021 0.0065 0.0112 0.0061 0.0069
Bud Site 0.0077 0.007 0.0015 0.0016 0.0014 0.0004 0.0205 0.039 0.0043 0.0008 0.0007 0.0005 0.0042 0.0222 0.004 0.0223 0.0248 0.0012
Cell Periphery 0.0027 0.0022 0.002 0.0014 0.0005 0.001 0.0025 0.0017 0.0009 0.0037 0.0017 0.0016 0.0023 0.0013 0.0009 0.0034 0.0012 0.0013
Cytoplasm 0.1345 0.2758 0.1393 0.2668 0.1257 0.4464 0.2787 0.4351 0.3853 0.324 0.5473 0.6365 0.2196 0.4487 0.254 0.2991 0.5132 0.5732
Cytoplasmic Foci 0.0157 0.0062 0.003 0.0065 0.037 0.0028 0.0321 0.0054 0.0177 0.0908 0.0119 0.0115 0.0163 0.0043 0.0168 0.0122 0.0138 0.0085
Eisosomes 0.0008 0.0004 0.0004 0.0008 0.0005 0.0005 0.0007 0.0003 0.0003 0.0012 0.0004 0.0002 0.0007 0.0001 0.0005 0.0002 0.0004 0.0001
Endoplasmic Reticulum 0.0054 0.0016 0.0011 0.0021 0.0032 0.0053 0.0044 0.0024 0.0027 0.0051 0.0044 0.0051 0.0041 0.002 0.0021 0.0034 0.0036 0.0034
Endosome 0.0132 0.0017 0.0006 0.0021 0.029 0.0052 0.0225 0.0005 0.0084 0.0612 0.0125 0.019 0.0101 0.0011 0.0047 0.0038 0.0423 0.002
Golgi 0.0053 0.0005 0.0008 0.0015 0.0152 0.0008 0.0096 0.0002 0.0087 0.0035 0.0055 0.0042 0.0039 0.0002 0.0033 0.0004 0.0107 0.0007
Lipid Particles 0.0063 0.0004 0.0022 0.0041 0.0544 0.001 0.022 0.0007 0.0044 0.0241 0.0088 0.0058 0.007 0.0003 0.0027 0.001 0.0177 0.0005
Mitochondria 0.0039 0.0011 0.0005 0.0081 0.0186 0.0006 0.0244 0.0005 0.0254 0.001 0.0042 0.0028 0.0053 0.0002 0.0061 0.0005 0.0065 0.0006
None 0.728 0.6868 0.8334 0.672 0.6027 0.5101 0.4963 0.4965 0.5062 0.4165 0.3694 0.2865 0.6511 0.509 0.6434 0.6142 0.2546 0.3704
Nuclear Periphery 0.015 0.0002 0.0001 0.0003 0.0063 0.0046 0.0039 0.0002 0.0006 0.0011 0.0031 0.0011 0.0048 0.0003 0.0008 0.0006 0.0065 0.001
Nucleolus 0.0008 0.0001 0.0001 0.0002 0.0005 0.0003 0.0013 0.0002 0.0006 0.0006 0.0003 0.0008 0.0008 0.0001 0.0011 0.001 0.0045 0.0002
Nucleus 0.0022 0.0003 0.0002 0.0004 0.0017 0.0013 0.0026 0.0006 0.0015 0.0007 0.001 0.0025 0.0041 0.0007 0.004 0.0023 0.0077 0.0017
Peroxisomes 0.0048 0.0005 0.0062 0.0201 0.0558 0.0003 0.0269 0.0003 0.0075 0.0124 0.0019 0.0021 0.0062 0.0001 0.0032 0.0003 0.0072 0.0003
Punctate Nuclear 0.0061 0.0002 0.0003 0.0004 0.0109 0.0013 0.0139 0.0004 0.0024 0.0183 0.0103 0.0008 0.0037 0.0003 0.0038 0.0016 0.0139 0.0016
Vacuole 0.0017 0.0095 0.0005 0.002 0.0013 0.0008 0.0047 0.001 0.0034 0.0074 0.0016 0.006 0.0029 0.0013 0.0022 0.0045 0.0037 0.0028
Vacuole Periphery 0.0012 0.0007 0 0.0001 0.0011 0.0003 0.0009 0.0001 0.0005 0.0009 0.0006 0.0008 0.0009 0.0001 0.0002 0.0003 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.4595 32.6514 21.6932 26.9959 9.0859 6.3672 36.2768 40.6778 22.4507 12.577
Translational Efficiency 0.7063 0.6071 0.8778 0.3752 0.5301 0.4602 0.5582 0.4873 0.5807 0.4809

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2237 2186 1249 1199 1258 1505 112 1761 3495 3691 1361 2960

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 680.51 750.59 966.99 979.69 716.08 677.18 1000.30 899.55 693.31 720.66 969.73 932.01
Standard Deviation 74.03 113.69 126.95 120.21 78.43 100.96 101.90 117.89 77.55 114.51 125.41 125.18
Intensity Change Log 2 — 0.141409 0.506885 0.525709 — -0.080581 0.482240 0.329083 — 0.031855 0.494301 0.428241

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000419 0.000606 0.000841 0.001187 0.000268 0.000941 0.000565 0.001576 0.000364 0.000743 0.000818 0.001418
Bud Neck 0.043190 0.039288 0.006573 0.005690 0.013909 0.042974 0.002665 0.005074 0.032651 0.040791 0.006252 0.005323
Bud Site 0.011917 0.021764 0.027875 0.061172 0.004913 0.024998 0.011567 0.051904 0.009396 0.023083 0.026533 0.055658
Cell Periphery 0.000602 0.000184 0.000318 0.000295 0.000324 0.000256 0.000714 0.000399 0.000502 0.000214 0.000350 0.000357
Cytoplasm 0.511610* 0.499268* 0.377091* 0.501042* 0.565375* 0.427428* 0.321583* 0.645634* 0.530962* 0.469976* 0.372523* 0.587064*
Cytoplasmic Foci 0.169614 0.182978* 0.019759 0.061948 0.206711* 0.261763* 0.085407 0.043808 0.182967* 0.215103* 0.025161 0.051156
Eisosomes 0.001081 0.000282 0.000061 0.000115 0.000505 0.000464 0.000241 0.000184 0.000874 0.000356 0.000076 0.000156
Endoplasmic Reticulum 0.001964 0.000904 0.006355 0.001669 0.000736 0.001091 0.005197 0.000645 0.001522 0.000980 0.006260 0.001060
Endosome 0.018450 0.027045 0.004650 0.008444 0.010942 0.041834 0.011432 0.002674 0.015747 0.033075 0.005208 0.005011
Golgi 0.005242 0.005435 0.000317 0.006166 0.002864 0.010001 0.000187 0.000246 0.004386 0.007297 0.000307 0.002644
Lipid Particles 0.012446 0.005464 0.002352 0.001104 0.005888 0.012754 0.005321 0.000720 0.010085 0.008436 0.002596 0.000875
Mitochondria 0.004114 0.004599 0.000757 0.000902 0.001688 0.008636 0.000109 0.000394 0.003241 0.006245 0.000704 0.000600
Mitotic Spindle 0.000716 0.003287 0.009039 0.026612 0.000857 0.003201 0.000220 0.002553 0.000767 0.003252 0.008313 0.012298
None 0.003501 0.001968 0.003269 0.000395 0.001689 0.002703 0.000557 0.002132 0.002849 0.002267 0.003046 0.001428
Nuclear Periphery 0.000201 0.000208 0.002375 0.000156 0.000189 0.000402 0.000849 0.000142 0.000197 0.000287 0.002250 0.000148
Nuclear Periphery Foci 0.000440 0.000326 0.004126 0.001957 0.000451 0.000451 0.010022 0.001281 0.000444 0.000377 0.004611 0.001555
Nucleolus 0.000999 0.001198 0.000338 0.000100 0.000653 0.001720 0.000324 0.000100 0.000874 0.001411 0.000337 0.000100
Nucleus 0.092779 0.066673 0.407177* 0.020628 0.115271 0.051193 0.133874* 0.037567 0.100875 0.060361 0.384686* 0.030706
Peroxisomes 0.004112 0.006534 0.000375 0.002230 0.003167 0.013866 0.000585 0.000543 0.003772 0.009524 0.000393 0.001227
Vacuole 0.115438 0.130339 0.125331 0.296355* 0.062645 0.090891 0.408392* 0.202080* 0.096436 0.114254 0.148625 0.240268*
Vacuole Periphery 0.001166 0.001648 0.001021 0.001832 0.000955 0.002433 0.000188 0.000345 0.001090 0.001968 0.000952 0.000948

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.54 -2.25 -4.22 -2.55 -0.03 -3.35 -3.20 -2.17 -0.49 -1.35 -3.44 -2.63 -2.83 -1.27 -0.50
Bud Neck 1.58 18.93 20.59 17.81 4.70 -10.60 9.57 8.32 15.39 -2.98 -3.91 19.36 21.82 23.54 4.80
Bud Site -6.30 -2.90 -8.78 -5.31 -4.89 -10.78 -3.58 -12.09 -3.71 -8.15 -11.31 -3.99 -13.90 -6.52 -6.04
Cell Periphery 16.09 8.49 14.30 -2.41 3.35 3.38 -4.30 0.69 -1.40 4.36 15.64 5.66 9.43 -2.74 2.34
Cytoplasm 1.80 22.96 6.11 4.39 -12.76 14.09 13.42 -1.16 -14.79 -13.84 10.27 27.92 0.75 -7.95 -24.60
Cytoplasmic Foci -2.61 43.31 31.36 31.32 -12.79 -7.55 16.02 34.28 38.37 4.33 -7.53 53.53 47.08 49.34 -11.72
Eisosomes 13.98 19.12 18.79 7.75 -2.64 0.83 5.28 5.81 5.84 2.31 12.44 20.82 16.38 7.06 -1.04
Endoplasmic Reticulum 3.21 -14.07 1.94 -1.02 16.31 -1.24 -6.78 -0.47 1.08 6.75 2.32 -17.27 2.53 0.06 20.20
Endosome -5.46 17.26 14.00 14.91 -0.48 -12.97 0.28 10.44 17.71 8.44 -12.81 16.90 19.86 23.87 3.95
Golgi -0.14 13.36 2.51 2.41 -2.56 -7.72 4.94 4.93 13.22 -0.22 -5.17 13.24 6.46 10.11 -2.48
Lipid Particles 8.46 12.56 15.21 12.18 10.11 -5.29 0.49 10.95 9.91 7.55 2.22 13.16 18.03 13.95 13.10
Mitochondria -0.51 5.95 6.27 6.86 1.57 -6.34 3.76 3.28 8.22 -2.43 -4.42 6.46 7.20 10.79 2.84
Mitotic Spindle -2.93 -4.56 -5.03 -3.96 -1.85 -2.14 1.37 -2.83 0.27 -4.77 -3.62 -4.54 -5.75 -3.29 0.14
None 3.24 1.83 7.14 5.10 11.42 -1.34 2.63 0.18 1.47 -2.64 1.26 0.97 4.92 2.87 5.62
Nuclear Periphery -0.29 -17.93 -2.77 -2.04 17.50 -2.02 -6.28 -4.21 1.20 5.60 -1.91 -18.40 -4.18 0.27 17.85
Nuclear Periphery Foci 3.82 -11.62 -11.21 -12.13 7.50 -0.35 -7.83 -8.96 -6.76 7.07 1.37 -13.25 -14.19 -13.74 9.98
Nucleolus -0.88 5.05 7.32 6.72 8.47 -3.14 1.99 3.72 4.99 3.42 -2.91 5.21 8.14 7.91 8.92
Nucleus 8.51 -36.28 21.19 11.79 43.24 14.34 -1.45 17.17 1.68 5.39 15.98 -34.25 26.33 9.42 41.83
Peroxisomes -5.25 14.00 7.16 11.13 -3.69 -10.98 6.75 7.05 15.00 1.32 -11.89 15.28 11.94 19.54 -3.73
Vacuole -4.05 -10.68 -31.32 -28.81 -18.82 -7.24 -16.52 -32.52 -26.89 7.06 -6.32 -16.86 -41.63 -37.19 -13.60
Vacuole Periphery -3.20 2.14 1.07 2.41 -0.59 -3.79 3.29 2.85 6.81 -0.43 -4.94 2.09 3.64 7.36 0.28
Standard name
Human Ortholog
Description Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (66%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Frk1

Frk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Frk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available