Standard name
Human Ortholog
Description Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.95 1.0 0.99 0.98 0.95 0.88 0.84 0.81 0.76 0.75 0.72 0.67 0.99 0.99 0.99 0.96 0.96 0.91 0.95 0.91 0.94 0.96 0.9 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.08 0.1 0.29 0.33 0.27 0.43 0.42 0.49 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 1 1 5 10 14 9 23 16 16 25 1 0 0 0 0 0 0 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 3 9 2 7 4 6 10 9 16 0 0 4 5 2 10 0 0 0 0 0 0
Cytoplasm 59 16 133 341 596 602 900 781 618 636 508 595 279 440 575 109 55 144 73 62 123 88 302 342
Endoplasmic Reticulum 0 0 0 1 2 2 3 1 0 2 1 3 1 4 4 3 0 6 0 0 1 0 1 1
Endosome 0 0 0 0 2 3 3 1 1 1 0 1 0 0 1 0 0 1 1 0 0 0 4 3
Golgi 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 3 0 0 1 0 1 2
Mitochondria 1 1 4 27 65 201 353 265 349 361 347 471 1 1 1 1 0 2 0 0 0 0 4 2
Nucleus 1 0 1 0 3 4 4 12 7 8 6 7 2 2 1 4 1 3 0 0 0 0 0 3
Nuclear Periphery 1 0 0 0 0 0 5 11 4 0 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 5 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 2 0 1 2 1 4 0 0 1 0 0 1 0 0 0 0 1 1
Vac/Vac Membrane 1 0 1 1 9 5 17 32 25 18 15 17 0 2 3 3 1 13 1 2 1 1 13 6
Unique Cell Count 62 16 135 347 629 682 1066 968 816 851 703 889 282 446 583 114 57 159 78 69 131 92 336 371
Labelled Cell Count 64 17 143 375 693 830 1314 1118 1034 1055 906 1144 284 449 590 126 59 184 78 69 131 92 336 371


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.2 5.9 5.4 5.2 4.4 4.3 4.4 3.9 4.0 3.8 3.7 7.0 6.9 6.9 9.7 10.2 10.0 6.7 6.9 6.9
Std Deviation (1e-4) 0.9 0.9 0.9 0.9 1.3 1.2 1.3 1.2 1.1 0.9 1.0 0.7 0.9 1.0 1.0 1.6 2.3 1.7 1.3 1.2 1.2
Intensity Change (Log2) – – – -0.13 -0.17 -0.41 -0.46 -0.4 -0.59 -0.55 -0.63 -0.67 0.26 0.24 0.23 0.73 0.8 0.78 0.19 0.24 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.1
Cytoplasm -0.2 -1.9 -3.6 -4.4 -5.2 -6.0 -6.2 -6.6 -7.5 0.4 0.1 0.1 -1.4 -0.9 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1473 3.237 3.1399 2.8804 2.5421 3.1898 1.4126 2.6648 2.4053 2.4066 2.1718 2.6436 3.0991 4.6691 4.5004 3.8875 4.3686 4.3833
Actin 0.0434 0.001 0.0164 0.014 0.0329 0.0077 0.0384 0.0015 0.0333 0.0024 0.0592 0.0063 0.0019 0.0004 0.0009 0.0003 0.0004 0.0001
Bud 0.0005 0.0001 0.0001 0.0036 0.0003 0.0001 0.0019 0.0001 0.0018 0.0006 0.001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0
Bud Neck 0.0007 0.0002 0.0008 0.0017 0.0008 0.0012 0.0062 0.0001 0.0005 0.0004 0.0009 0.0013 0.0002 0.0001 0.0004 0.0002 0.0004 0.0004
Bud Periphery 0.0008 0.0001 0.0001 0.0027 0.0003 0.0002 0.0017 0 0.0016 0.0011 0.0014 0.0004 0 0 0.0001 0 0.0001 0
Bud Site 0.0036 0.0007 0.0007 0.0211 0.0009 0.0002 0.0191 0.0002 0.0032 0.0009 0.0033 0.0003 0.0002 0.0008 0.0026 0.0001 0.0002 0
Cell Periphery 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0009 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Cytoplasm 0.7109 0.886 0.8924 0.8095 0.8304 0.9316 0.732 0.9344 0.8499 0.8112 0.5879 0.9095 0.8725 0.9587 0.9488 0.9788 0.96 0.9693
Cytoplasmic Foci 0.018 0.0043 0.0043 0.0267 0.0162 0.0067 0.0397 0.0065 0.0119 0.0074 0.0581 0.0097 0.0054 0.0033 0.0038 0.0038 0.0066 0.0047
Eisosomes 0.0009 0 0.0003 0.0001 0.0005 0.0001 0.0008 0 0.0001 0 0.0004 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.006 0.0062 0.0028 0.0042 0.0045 0.0017 0.0063 0.0029 0.003 0.0026 0.0024 0.0015 0.0067 0.0015 0.0023 0.0025 0.0022 0.0016
Endosome 0.0141 0.0031 0.0032 0.0057 0.0323 0.0085 0.0226 0.0078 0.0221 0.051 0.083 0.009 0.0029 0.0012 0.0019 0.0038 0.0021 0.0035
Golgi 0.0033 0.0017 0.0006 0.0018 0.0073 0.0034 0.0106 0.001 0.0099 0.0172 0.0286 0.003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Lipid Particles 0.0041 0.0017 0.0004 0.0004 0.0207 0.0037 0.0168 0.0001 0.0026 0.0061 0.0154 0.0025 0.0006 0.0002 0.0001 0 0 0
Mitochondria 0.0037 0.007 0.0008 0.0008 0.0029 0.0022 0.0129 0.0001 0.0212 0.0265 0.0761 0.0043 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
None 0.1647 0.0822 0.0717 0.0916 0.0123 0.0256 0.0434 0.0428 0.0141 0.06 0.0067 0.0307 0.1037 0.0307 0.0326 0.0064 0.0212 0.0137
Nuclear Periphery 0.0043 0.0012 0.0013 0.0015 0.015 0.0018 0.0032 0.0008 0.0078 0.0012 0.0015 0.0066 0.0016 0.0004 0.0008 0.0007 0.0009 0.0008
Nucleolus 0.0009 0.0001 0.0001 0.0002 0.0005 0.0001 0.0064 0 0.0001 0.0002 0.0009 0.0013 0 0 0 0 0 0
Nucleus 0.0028 0.0016 0.0022 0.0025 0.0054 0.0017 0.0029 0.0008 0.0024 0.0017 0.0013 0.0062 0.002 0.0016 0.0038 0.0018 0.0037 0.0045
Peroxisomes 0.0088 0.0013 0.0003 0.0038 0.0074 0.0008 0.0159 0.0002 0.0092 0.0006 0.0578 0.0027 0.0001 0.0001 0.0001 0 0.0002 0
Punctate Nuclear 0.0058 0.0004 0.0003 0.0064 0.0067 0.0016 0.0049 0.0002 0.0011 0.0003 0.0087 0.0028 0.0006 0.0002 0.0005 0.0002 0.0008 0.0004
Vacuole 0.0021 0.0009 0.0009 0.0011 0.0013 0.0006 0.0114 0.0005 0.0022 0.0063 0.003 0.001 0.0007 0.0004 0.0006 0.0007 0.0005 0.0005
Vacuole Periphery 0.0004 0.0002 0.0002 0.0003 0.0011 0.0003 0.0022 0.0001 0.0018 0.0024 0.0023 0.0006 0.0001 0 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5766 11.0333 13.0995 14.8586 14.4021 12.3355 23.9849 26.3528 25.1538 25.9847
Translational Efficiency 1.3493 2.7708 1.4943 1.4198 1.4682 1.9565 1.5626 1.403 1.3537 1.4335

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1786 1039 1012 742 1921 1419 1612 1293 3707 2458 2624 2035

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 759.83 789.04 1037.05 993.01 762.76 837.04 975.62 942.18 761.35 816.75 999.31 960.71
Standard Deviation 277.07 102.46 131.42 129.02 104.85 105.94 106.14 125.66 206.60 107.14 120.31 129.23
Intensity Change Log 2 — 0.054422 0.448737 0.386132 — 0.134067 0.355090 0.304774 — 0.094871 0.402583 0.345948

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000067 0.000192 0.000269 0.001625 0.000092 0.000215 0.000778 0.000689 0.000080 0.000205 0.000582 0.001031
Bud Neck 0.003598 0.005561 0.001111 0.007240 0.006455 0.011400 0.002498 0.011271 0.005078 0.008932 0.001963 0.009801
Bud Site 0.002433 0.007477 0.007426 0.054362 0.004097 0.012739 0.008590 0.023415 0.003295 0.010515 0.008141 0.034699
Cell Periphery 0.000099 0.000060 0.000095 0.000132 0.000073 0.000074 0.000060 0.000089 0.000086 0.000068 0.000073 0.000104
Cytoplasm 0.690335* 0.704587* 0.720646* 0.544639* 0.651199* 0.613189* 0.681800* 0.669835* 0.670054* 0.651823* 0.696782* 0.624186*
Cytoplasmic Foci 0.141544 0.122443 0.018063 0.013386 0.139249 0.152551 0.008102 0.018828 0.140354 0.139824 0.011943 0.016844
Eisosomes 0.000050 0.000029 0.000017 0.000027 0.000058 0.000058 0.000013 0.000020 0.000054 0.000046 0.000014 0.000022
Endoplasmic Reticulum 0.000838 0.000589 0.001394 0.001703 0.000605 0.000540 0.001006 0.000662 0.000717 0.000561 0.001156 0.001042
Endosome 0.007579 0.009251 0.002199 0.006308 0.008738 0.016313 0.001614 0.005876 0.008180 0.013328 0.001840 0.006033
Golgi 0.001195 0.001764 0.000175 0.015800 0.001174 0.001989 0.000688 0.002846 0.001184 0.001894 0.000490 0.007569
Lipid Particles 0.002929 0.001258 0.000727 0.000707 0.002520 0.002362 0.001331 0.000541 0.002717 0.001895 0.001098 0.000602
Mitochondria 0.001169 0.000519 0.000190 0.001560 0.001580 0.001994 0.000415 0.014790 0.001382 0.001371 0.000328 0.009966
Mitotic Spindle 0.001089 0.001762 0.004132 0.047572 0.002194 0.006773 0.008168 0.017606 0.001662 0.004655 0.006611 0.028532
None 0.005234 0.003351 0.005571 0.005299 0.001443 0.001448 0.006027 0.007052 0.003270 0.002252 0.005851 0.006413
Nuclear Periphery 0.000217 0.000180 0.000457 0.000763 0.000264 0.000266 0.000608 0.000549 0.000241 0.000229 0.000550 0.000627
Nuclear Periphery Foci 0.000775 0.001251 0.003858 0.002257 0.000658 0.000522 0.001486 0.001127 0.000714 0.000830 0.002401 0.001539
Nucleolus 0.000590 0.000596 0.000112 0.000223 0.000399 0.000656 0.000101 0.000262 0.000491 0.000631 0.000105 0.000248
Nucleus 0.089693 0.062104 0.168624* 0.141810* 0.087454 0.060731 0.222377* 0.101416 0.088533 0.061312 0.201646* 0.116144
Peroxisomes 0.000940 0.001838 0.000250 0.000746 0.001004 0.004121 0.000417 0.003667 0.000973 0.003156 0.000352 0.002602
Vacuole 0.048701 0.074364 0.064555 0.141464 0.090050 0.110651 0.053823 0.113107 0.070128 0.095312 0.057962 0.123447
Vacuole Periphery 0.000925 0.000825 0.000131 0.012377 0.000696 0.001406 0.000098 0.006354 0.000806 0.001161 0.000110 0.008550

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.00 -12.41 -1.79 -1.48 -1.12 -4.84 -1.89 -2.50 -1.16 0.60 -6.39 -2.81 -2.59 -1.82 -0.45
Bud Neck -2.72 7.08 0.06 2.34 -3.87 -4.15 5.63 1.80 4.95 -1.81 -5.20 7.27 1.37 5.22 -2.84
Bud Site -5.91 -3.76 -5.05 -3.22 -3.09 -6.46 -1.44 -3.46 2.13 -1.83 -8.53 -3.62 -6.27 -0.94 -3.50
Cell Periphery 6.90 -1.56 0.13 -4.54 1.35 0.05 0.58 2.89 2.98 2.84 4.72 -0.15 2.68 -1.46 2.86
Cytoplasm -0.63 4.53 23.68 21.81 17.94 4.40 8.23 10.54 5.56 3.11 3.50 10.02 22.61 17.28 13.05
Cytoplasmic Foci 3.36 31.68 34.00 22.52 5.91 -2.35 36.80 34.33 30.47 -6.61 0.28 48.97 48.11 37.62 -0.99
Eisosomes 8.81 11.79 4.98 -1.85 -4.02 -0.08 11.88 10.31 11.54 -4.54 3.26 16.84 12.23 8.58 -5.74
Endoplasmic Reticulum 0.99 -3.92 -3.56 -5.82 -0.37 0.59 -9.13 -2.65 -2.57 7.63 1.17 -6.93 -3.61 -6.22 3.55
Endosome -1.81 9.25 8.19 8.25 0.80 -6.04 13.48 11.37 12.36 -1.83 -6.23 16.35 14.00 14.79 -0.96
Golgi -0.95 5.98 -1.05 -0.70 -1.70 -2.19 3.33 1.56 3.56 -1.44 -2.19 5.68 -0.48 0.56 -2.04
Lipid Particles 6.40 7.69 6.67 -0.04 -2.52 0.55 3.68 8.20 5.19 1.18 3.56 6.53 10.91 5.12 0.72
Mitochondria 2.79 4.17 1.72 -1.49 -3.93 -0.63 2.90 -1.39 -1.10 -2.40 0.14 4.44 -1.25 -1.30 -2.63
Mitotic Spindle -0.77 -2.73 -6.08 -5.95 -5.28 -2.89 -3.30 -3.80 -0.93 -0.75 -3.05 -4.30 -7.22 -5.34 -4.60
None 2.49 0.50 0.01 -3.04 -0.60 0.01 -14.88 -8.92 -8.95 0.65 2.74 -5.20 -4.48 -8.21 0.10
Nuclear Periphery 0.90 -13.49 -17.28 -18.04 -10.44 -0.22 -19.83 -12.75 -12.04 3.57 0.07 -24.46 -21.16 -20.93 -4.26
Nuclear Periphery Foci -2.96 -11.20 -4.31 -2.61 4.24 0.61 -5.50 -2.26 -6.18 5.24 -1.26 -12.12 -4.92 -4.28 6.28
Nucleolus -0.37 8.61 7.04 3.73 -4.66 -3.41 6.66 2.89 5.20 -2.08 -2.43 10.83 6.28 6.24 -2.96
Nucleus 7.27 -16.56 -14.00 -17.24 -0.37 7.47 -30.00 -12.46 -17.57 15.58 10.48 -33.92 -18.50 -24.43 12.01
Peroxisomes -4.30 5.39 5.28 8.40 -0.32 -6.53 7.00 0.26 4.74 -1.33 -7.49 8.52 1.02 5.89 -1.36
Vacuole -7.64 -11.12 -24.21 -19.90 -16.22 -4.75 -5.07 -19.69 -15.35 -15.14 -8.90 -11.90 -31.39 -24.62 -21.91
Vacuole Periphery 0.65 4.84 -1.07 -1.18 -1.72 -2.81 8.23 -1.16 -0.09 -2.15 -1.93 8.02 -1.52 -0.98 -2.71
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam)
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Poc4

Poc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Poc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available