Standard name
Human Ortholog
Description Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.95 1.0 0.99 0.98 0.95 0.88 0.84 0.81 0.76 0.75 0.72 0.67 0.99 0.99 0.99 0.96 0.96 0.91 0.95 0.91 0.94 0.96 0.9 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.08 0.1 0.29 0.33 0.27 0.43 0.42 0.49 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 1 1 5 10 14 9 23 16 16 25 1 0 0 0 0 0 0 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 3 9 2 7 4 6 10 9 16 0 0 4 5 2 10 0 0 0 0 0 0
Cytoplasm 59 16 133 341 596 602 900 781 618 636 508 595 279 440 575 109 55 144 73 62 123 88 302 342
Endoplasmic Reticulum 0 0 0 1 2 2 3 1 0 2 1 3 1 4 4 3 0 6 0 0 1 0 1 1
Endosome 0 0 0 0 2 3 3 1 1 1 0 1 0 0 1 0 0 1 1 0 0 0 4 3
Golgi 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 3 0 0 1 0 1 2
Mitochondria 1 1 4 27 65 201 353 265 349 361 347 471 1 1 1 1 0 2 0 0 0 0 4 2
Nucleus 1 0 1 0 3 4 4 12 7 8 6 7 2 2 1 4 1 3 0 0 0 0 0 3
Nuclear Periphery 1 0 0 0 0 0 5 11 4 0 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 5 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 2 0 1 2 1 4 0 0 1 0 0 1 0 0 0 0 1 1
Vac/Vac Membrane 1 0 1 1 9 5 17 32 25 18 15 17 0 2 3 3 1 13 1 2 1 1 13 6
Unique Cell Count 62 16 135 347 629 682 1066 968 816 851 703 889 282 446 583 114 57 159 78 69 131 92 336 371
Labelled Cell Count 64 17 143 375 693 830 1314 1118 1034 1055 906 1144 284 449 590 126 59 184 78 69 131 92 336 371


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.2 5.9 5.4 5.2 4.4 4.3 4.4 3.9 4.0 3.8 3.7 7.0 6.9 6.9 9.7 10.2 10.0 6.7 6.9 6.9
Std Deviation (1e-4) 0.9 0.9 0.9 0.9 1.3 1.2 1.3 1.2 1.1 0.9 1.0 0.7 0.9 1.0 1.0 1.6 2.3 1.7 1.3 1.2 1.2
Intensity Change (Log2) – – – -0.13 -0.17 -0.41 -0.46 -0.4 -0.59 -0.55 -0.63 -0.67 0.26 0.24 0.23 0.73 0.8 0.78 0.19 0.24 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.1
Cytoplasm -0.2 -1.9 -3.6 -4.4 -5.2 -6.0 -6.2 -6.6 -7.5 0.4 0.1 0.1 -1.4 -0.9 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1473 3.237 3.1399 2.8804 2.5421 3.1898 1.4126 2.6648 2.4053 2.4066 2.1718 2.6436 3.0991 4.6691 4.5004 3.8875 4.3686 4.3833
Actin 0.0434 0.001 0.0164 0.014 0.0329 0.0077 0.0384 0.0015 0.0333 0.0024 0.0592 0.0063 0.0019 0.0004 0.0009 0.0003 0.0004 0.0001
Bud 0.0005 0.0001 0.0001 0.0036 0.0003 0.0001 0.0019 0.0001 0.0018 0.0006 0.001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0
Bud Neck 0.0007 0.0002 0.0008 0.0017 0.0008 0.0012 0.0062 0.0001 0.0005 0.0004 0.0009 0.0013 0.0002 0.0001 0.0004 0.0002 0.0004 0.0004
Bud Periphery 0.0008 0.0001 0.0001 0.0027 0.0003 0.0002 0.0017 0 0.0016 0.0011 0.0014 0.0004 0 0 0.0001 0 0.0001 0
Bud Site 0.0036 0.0007 0.0007 0.0211 0.0009 0.0002 0.0191 0.0002 0.0032 0.0009 0.0033 0.0003 0.0002 0.0008 0.0026 0.0001 0.0002 0
Cell Periphery 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0009 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0
Cytoplasm 0.7109 0.886 0.8924 0.8095 0.8304 0.9316 0.732 0.9344 0.8499 0.8112 0.5879 0.9095 0.8725 0.9587 0.9488 0.9788 0.96 0.9693
Cytoplasmic Foci 0.018 0.0043 0.0043 0.0267 0.0162 0.0067 0.0397 0.0065 0.0119 0.0074 0.0581 0.0097 0.0054 0.0033 0.0038 0.0038 0.0066 0.0047
Eisosomes 0.0009 0 0.0003 0.0001 0.0005 0.0001 0.0008 0 0.0001 0 0.0004 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.006 0.0062 0.0028 0.0042 0.0045 0.0017 0.0063 0.0029 0.003 0.0026 0.0024 0.0015 0.0067 0.0015 0.0023 0.0025 0.0022 0.0016
Endosome 0.0141 0.0031 0.0032 0.0057 0.0323 0.0085 0.0226 0.0078 0.0221 0.051 0.083 0.009 0.0029 0.0012 0.0019 0.0038 0.0021 0.0035
Golgi 0.0033 0.0017 0.0006 0.0018 0.0073 0.0034 0.0106 0.001 0.0099 0.0172 0.0286 0.003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Lipid Particles 0.0041 0.0017 0.0004 0.0004 0.0207 0.0037 0.0168 0.0001 0.0026 0.0061 0.0154 0.0025 0.0006 0.0002 0.0001 0 0 0
Mitochondria 0.0037 0.007 0.0008 0.0008 0.0029 0.0022 0.0129 0.0001 0.0212 0.0265 0.0761 0.0043 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
None 0.1647 0.0822 0.0717 0.0916 0.0123 0.0256 0.0434 0.0428 0.0141 0.06 0.0067 0.0307 0.1037 0.0307 0.0326 0.0064 0.0212 0.0137
Nuclear Periphery 0.0043 0.0012 0.0013 0.0015 0.015 0.0018 0.0032 0.0008 0.0078 0.0012 0.0015 0.0066 0.0016 0.0004 0.0008 0.0007 0.0009 0.0008
Nucleolus 0.0009 0.0001 0.0001 0.0002 0.0005 0.0001 0.0064 0 0.0001 0.0002 0.0009 0.0013 0 0 0 0 0 0
Nucleus 0.0028 0.0016 0.0022 0.0025 0.0054 0.0017 0.0029 0.0008 0.0024 0.0017 0.0013 0.0062 0.002 0.0016 0.0038 0.0018 0.0037 0.0045
Peroxisomes 0.0088 0.0013 0.0003 0.0038 0.0074 0.0008 0.0159 0.0002 0.0092 0.0006 0.0578 0.0027 0.0001 0.0001 0.0001 0 0.0002 0
Punctate Nuclear 0.0058 0.0004 0.0003 0.0064 0.0067 0.0016 0.0049 0.0002 0.0011 0.0003 0.0087 0.0028 0.0006 0.0002 0.0005 0.0002 0.0008 0.0004
Vacuole 0.0021 0.0009 0.0009 0.0011 0.0013 0.0006 0.0114 0.0005 0.0022 0.0063 0.003 0.001 0.0007 0.0004 0.0006 0.0007 0.0005 0.0005
Vacuole Periphery 0.0004 0.0002 0.0002 0.0003 0.0011 0.0003 0.0022 0.0001 0.0018 0.0024 0.0023 0.0006 0.0001 0 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5766 11.0333 13.0995 14.8586 14.4021 12.3355 23.9849 26.3528 25.1538 25.9847
Translational Efficiency 1.3493 2.7708 1.4943 1.4198 1.4682 1.9565 1.5626 1.403 1.3537 1.4335

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1786 1039 1012 742 1921 1419 1612 1293 3707 2458 2624 2035

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 759.83 789.04 1037.05 993.01 762.76 837.04 975.62 942.18 761.35 816.75 999.31 960.71
Standard Deviation 277.07 102.46 131.42 129.02 104.85 105.94 106.14 125.66 206.60 107.14 120.31 129.23
Intensity Change Log 2 — 0.054422 0.448737 0.386132 — 0.134067 0.355090 0.304774 — 0.094871 0.402583 0.345948

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000067 0.000192 0.000269 0.001625 0.000092 0.000215 0.000778 0.000689 0.000080 0.000205 0.000582 0.001031
Bud Neck 0.003598 0.005561 0.001111 0.007240 0.006455 0.011400 0.002498 0.011271 0.005078 0.008932 0.001963 0.009801
Bud Site 0.002433 0.007477 0.007426 0.054362 0.004097 0.012739 0.008590 0.023415 0.003295 0.010515 0.008141 0.034699
Cell Periphery 0.000099 0.000060 0.000095 0.000132 0.000073 0.000074 0.000060 0.000089 0.000086 0.000068 0.000073 0.000104
Cytoplasm 0.690335* 0.704587* 0.720646* 0.544639* 0.651199* 0.613189* 0.681800* 0.669835* 0.670054* 0.651823* 0.696782* 0.624186*
Cytoplasmic Foci 0.141544 0.122443 0.018063 0.013386 0.139249 0.152551 0.008102 0.018828 0.140354 0.139824 0.011943 0.016844
Eisosomes 0.000050 0.000029 0.000017 0.000027 0.000058 0.000058 0.000013 0.000020 0.000054 0.000046 0.000014 0.000022
Endoplasmic Reticulum 0.000838 0.000589 0.001394 0.001703 0.000605 0.000540 0.001006 0.000662 0.000717 0.000561 0.001156 0.001042
Endosome 0.007579 0.009251 0.002199 0.006308 0.008738 0.016313 0.001614 0.005876 0.008180 0.013328 0.001840 0.006033
Golgi 0.001195 0.001764 0.000175 0.015800 0.001174 0.001989 0.000688 0.002846 0.001184 0.001894 0.000490 0.007569
Lipid Particles 0.002929 0.001258 0.000727 0.000707 0.002520 0.002362 0.001331 0.000541 0.002717 0.001895 0.001098 0.000602
Mitochondria 0.001169 0.000519 0.000190 0.001560 0.001580 0.001994 0.000415 0.014790 0.001382 0.001371 0.000328 0.009966
Mitotic Spindle 0.001089 0.001762 0.004132 0.047572 0.002194 0.006773 0.008168 0.017606 0.001662 0.004655 0.006611 0.028532
None 0.005234 0.003351 0.005571 0.005299 0.001443 0.001448 0.006027 0.007052 0.003270 0.002252 0.005851 0.006413
Nuclear Periphery 0.000217 0.000180 0.000457 0.000763 0.000264 0.000266 0.000608 0.000549 0.000241 0.000229 0.000550 0.000627
Nuclear Periphery Foci 0.000775 0.001251 0.003858 0.002257 0.000658 0.000522 0.001486 0.001127 0.000714 0.000830 0.002401 0.001539
Nucleolus 0.000590 0.000596 0.000112 0.000223 0.000399 0.000656 0.000101 0.000262 0.000491 0.000631 0.000105 0.000248
Nucleus 0.089693 0.062104 0.168624* 0.141810* 0.087454 0.060731 0.222377* 0.101416 0.088533 0.061312 0.201646* 0.116144
Peroxisomes 0.000940 0.001838 0.000250 0.000746 0.001004 0.004121 0.000417 0.003667 0.000973 0.003156 0.000352 0.002602
Vacuole 0.048701 0.074364 0.064555 0.141464 0.090050 0.110651 0.053823 0.113107 0.070128 0.095312 0.057962 0.123447
Vacuole Periphery 0.000925 0.000825 0.000131 0.012377 0.000696 0.001406 0.000098 0.006354 0.000806 0.001161 0.000110 0.008550

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.00 -12.41 -1.79 -1.48 -1.12 -4.84 -1.89 -2.50 -1.16 0.60 -6.39 -2.81 -2.59 -1.82 -0.45
Bud Neck -2.72 7.08 0.06 2.34 -3.87 -4.15 5.63 1.80 4.95 -1.81 -5.20 7.27 1.37 5.22 -2.84
Bud Site -5.91 -3.76 -5.05 -3.22 -3.09 -6.46 -1.44 -3.46 2.13 -1.83 -8.53 -3.62 -6.27 -0.94 -3.50
Cell Periphery 6.90 -1.56 0.13 -4.54 1.35 0.05 0.58 2.89 2.98 2.84 4.72 -0.15 2.68 -1.46 2.86
Cytoplasm -0.63 4.53 23.68 21.81 17.94 4.40 8.23 10.54 5.56 3.11 3.50 10.02 22.61 17.28 13.05
Cytoplasmic Foci 3.36 31.68 34.00 22.52 5.91 -2.35 36.80 34.33 30.47 -6.61 0.28 48.97 48.11 37.62 -0.99
Eisosomes 8.81 11.79 4.98 -1.85 -4.02 -0.08 11.88 10.31 11.54 -4.54 3.26 16.84 12.23 8.58 -5.74
Endoplasmic Reticulum 0.99 -3.92 -3.56 -5.82 -0.37 0.59 -9.13 -2.65 -2.57 7.63 1.17 -6.93 -3.61 -6.22 3.55
Endosome -1.81 9.25 8.19 8.25 0.80 -6.04 13.48 11.37 12.36 -1.83 -6.23 16.35 14.00 14.79 -0.96
Golgi -0.95 5.98 -1.05 -0.70 -1.70 -2.19 3.33 1.56 3.56 -1.44 -2.19 5.68 -0.48 0.56 -2.04
Lipid Particles 6.40 7.69 6.67 -0.04 -2.52 0.55 3.68 8.20 5.19 1.18 3.56 6.53 10.91 5.12 0.72
Mitochondria 2.79 4.17 1.72 -1.49 -3.93 -0.63 2.90 -1.39 -1.10 -2.40 0.14 4.44 -1.25 -1.30 -2.63
Mitotic Spindle -0.77 -2.73 -6.08 -5.95 -5.28 -2.89 -3.30 -3.80 -0.93 -0.75 -3.05 -4.30 -7.22 -5.34 -4.60
None 2.49 0.50 0.01 -3.04 -0.60 0.01 -14.88 -8.92 -8.95 0.65 2.74 -5.20 -4.48 -8.21 0.10
Nuclear Periphery 0.90 -13.49 -17.28 -18.04 -10.44 -0.22 -19.83 -12.75 -12.04 3.57 0.07 -24.46 -21.16 -20.93 -4.26
Nuclear Periphery Foci -2.96 -11.20 -4.31 -2.61 4.24 0.61 -5.50 -2.26 -6.18 5.24 -1.26 -12.12 -4.92 -4.28 6.28
Nucleolus -0.37 8.61 7.04 3.73 -4.66 -3.41 6.66 2.89 5.20 -2.08 -2.43 10.83 6.28 6.24 -2.96
Nucleus 7.27 -16.56 -14.00 -17.24 -0.37 7.47 -30.00 -12.46 -17.57 15.58 10.48 -33.92 -18.50 -24.43 12.01
Peroxisomes -4.30 5.39 5.28 8.40 -0.32 -6.53 7.00 0.26 4.74 -1.33 -7.49 8.52 1.02 5.89 -1.36
Vacuole -7.64 -11.12 -24.21 -19.90 -16.22 -4.75 -5.07 -19.69 -15.35 -15.14 -8.90 -11.90 -31.39 -24.62 -21.91
Vacuole Periphery 0.65 4.84 -1.07 -1.18 -1.72 -2.81 8.23 -1.16 -0.09 -2.15 -1.93 8.02 -1.52 -0.98 -2.71
Standard name
Human Ortholog
Description Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam)
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Poc4

Poc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Poc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available