Standard name | |
---|---|
Human Ortholog | |
Description | One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 4 | 4 | 8 | 21 | 27 | 22 | 29 | 13 | 15 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 2 |
Bud | 0 | 4 | 4 | 5 | 7 | 11 | 25 | 25 | 18 | 25 | 12 | 8 | 3 | 4 | 4 | 2 | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 5 | 20 | 10 | 12 | 37 | 46 | 46 | 54 | 53 | 36 | 34 | 27 | 23 | 13 | 4 | 14 | 2 | 0 | 0 | 3 | 0 | 0 | 0 |
Cytoplasm | 157 | 341 | 516 | 394 | 545 | 697 | 736 | 736 | 701 | 770 | 378 | 499 | 557 | 715 | 653 | 328 | 393 | 335 | 162 | 329 | 491 | 380 | 559 | 509 |
Endoplasmic Reticulum | 8 | 16 | 49 | 45 | 54 | 91 | 146 | 131 | 142 | 148 | 54 | 92 | 24 | 25 | 23 | 4 | 23 | 6 | 5 | 8 | 56 | 7 | 16 | 23 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 7 |
Golgi | 0 | 1 | 6 | 10 | 7 | 5 | 5 | 4 | 4 | 5 | 3 | 0 | 1 | 0 | 0 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2 | 0 | 2 | 13 | 13 | 5 | 7 | 10 | 7 | 0 | 0 | 0 | 6 | 0 | 2 | 0 | 0 | 3 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 5 | 2 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 4 | 1 | 7 | 6 | 5 | 6 | 3 | 6 | 1 | 1 | 2 | 6 | 5 | 8 | 0 | 1 | 2 | 13 | 6 | 7 |
Unique Cell Count | 164 | 350 | 562 | 445 | 597 | 790 | 918 | 910 | 870 | 961 | 457 | 612 | 576 | 735 | 671 | 344 | 402 | 344 | 178 | 346 | 567 | 420 | 610 | 564 |
Labelled Cell Count | 169 | 368 | 596 | 473 | 634 | 853 | 1000 | 989 | 953 | 1051 | 509 | 663 | 613 | 770 | 697 | 357 | 438 | 355 | 178 | 346 | 567 | 420 | 610 | 564 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 31.1 | 36.5 | 34.7 | 29.6 | 29.2 | 29.0 | 27.1 | 27.1 | 27.8 | 27.7 | 25.3 | 25.6 | 38.1 | 39.4 | 36.5 | 39.7 | 41.9 | 39.7 | 33.3 | 36.0 | 37.5 |
Std Deviation (1e-4) | 6.8 | 6.5 | 7.7 | 5.9 | 5.2 | 5.0 | 5.3 | 5.3 | 5.4 | 5.9 | 6.2 | 6.1 | 7.7 | 7.5 | 7.3 | 14.0 | 12.0 | 13.7 | 7.3 | 8.1 | 8.6 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.25 | -0.26 | -0.35 | -0.35 | -0.32 | -0.32 | -0.46 | -0.44 | 0.14 | 0.18 | 0.07 | 0.19 | 0.27 | 0.19 | -0.06 | 0.05 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.3 | 3.8 | 3.5 | 3.8 | 3.6 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0.8 | 1.2 | 2.7 | 2.7 | 2.0 | 2.6 | 2.4 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.2 | -1.6 | 1.0 | 1.3 | 1.3 | 2.2 | 1.7 | 3.0 | 1.6 | 1.0 | -0.4 | -1.8 | -2.2 | -0.1 | -2.8 |
Cytoplasm | -1.7 | -0.3 | -2.1 | -6.0 | -5.7 | -5.8 | -6.1 | -4.4 | -5.1 | 3.6 | 4.4 | 4.3 | 2.0 | 3.9 | 3.4 |
Endoplasmic Reticulum | 0.8 | 0.2 | 1.7 | 4.0 | 3.2 | 4.1 | 3.7 | 1.6 | 3.3 | -3.1 | -4.1 | -3.9 | -4.7 | -1.7 | -4.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.5 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 36.8377 | 41.5941 | 40.5871 | 38.6696 | 37.6732 | 40.7307 | 34.1084 | 46.49 | 43.1468 | 41.8955 | 40.9014 | 42.3718 | 38.3383 | 44.2062 | 42.7874 | 39.5121 | 37.2823 | 41.7879 |
Actin | 0.0023 | 0.004 | 0.0064 | 0.0323 | 0.0093 | 0.0027 | 0.0096 | 0.0004 | 0.0058 | 0.0093 | 0.0002 | 0.0042 | 0.0072 | 0.002 | 0.0011 | 0.0107 | 0.0234 | 0.0005 |
Bud | 0.0005 | 0.0002 | 0.0012 | 0.0002 | 0.0013 | 0.0002 | 0.0012 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0.0008 | 0.0014 | 0.0019 | 0.0001 |
Bud Neck | 0.0006 | 0.0005 | 0.0007 | 0.0017 | 0.0012 | 0.0102 | 0.003 | 0.0008 | 0.0019 | 0.0008 | 0.001 | 0.0101 | 0.0019 | 0.0019 | 0.0011 | 0.0013 | 0.0041 | 0.0066 |
Bud Periphery | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0012 | 0.0001 | 0.0014 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0006 | 0.0008 | 0.0033 | 0 |
Bud Site | 0.0008 | 0.0005 | 0.0005 | 0.0002 | 0.0074 | 0.0001 | 0.0046 | 0.0092 | 0.0009 | 0.0003 | 0 | 0.0002 | 0.001 | 0.0034 | 0.0003 | 0.0007 | 0.0049 | 0 |
Cell Periphery | 0.0015 | 0.0019 | 0.0009 | 0.0014 | 0.0002 | 0.0015 | 0.0076 | 0.0035 | 0.0009 | 0.0003 | 0.0002 | 0.0007 | 0.0055 | 0.0032 | 0.0008 | 0.0021 | 0.0038 | 0.0004 |
Cytoplasm | 0.8483 | 0.9379 | 0.9324 | 0.9011 | 0.8715 | 0.9047 | 0.767 | 0.9399 | 0.9128 | 0.9025 | 0.9519 | 0.9067 | 0.8256 | 0.925 | 0.9378 | 0.8725 | 0.8381 | 0.9185 |
Cytoplasmic Foci | 0.0117 | 0.0088 | 0.0041 | 0.0069 | 0.0095 | 0.0033 | 0.0142 | 0.005 | 0.0113 | 0.0074 | 0.0048 | 0.003 | 0.0057 | 0.0032 | 0.0031 | 0.0069 | 0.0054 | 0.0035 |
Eisosomes | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0008 | 0.0028 | 0 | 0.0001 | 0.0008 | 0 |
Endoplasmic Reticulum | 0.069 | 0.0158 | 0.0214 | 0.0197 | 0.004 | 0.0543 | 0.0506 | 0.0186 | 0.0153 | 0.0199 | 0.0095 | 0.0428 | 0.0493 | 0.0219 | 0.0261 | 0.0133 | 0.0039 | 0.0606 |
Endosome | 0.0095 | 0.0025 | 0.0059 | 0.0045 | 0.0249 | 0.0051 | 0.021 | 0.0015 | 0.0073 | 0.0046 | 0.0142 | 0.0053 | 0.0089 | 0.002 | 0.0056 | 0.0093 | 0.0071 | 0.0027 |
Golgi | 0.0015 | 0.0008 | 0.0005 | 0.0021 | 0.0046 | 0.0015 | 0.0039 | 0.0002 | 0.002 | 0.0008 | 0.0008 | 0.0016 | 0.0016 | 0.0004 | 0.0003 | 0.0017 | 0.0053 | 0.0003 |
Lipid Particles | 0.0033 | 0.0058 | 0.0008 | 0.0019 | 0.0073 | 0.0018 | 0.0176 | 0.0075 | 0.0044 | 0.0026 | 0.0002 | 0.0011 | 0.0069 | 0.0023 | 0.0006 | 0.002 | 0.0155 | 0.0001 |
Mitochondria | 0.0009 | 0.0007 | 0.0008 | 0.0005 | 0.0021 | 0.0004 | 0.009 | 0 | 0.0066 | 0.0003 | 0.0009 | 0.0067 | 0.0019 | 0.0002 | 0.0041 | 0.0124 | 0.018 | 0.0001 |
None | 0.0018 | 0.0036 | 0.0007 | 0.002 | 0.0019 | 0.0002 | 0.0057 | 0.0002 | 0.004 | 0.0013 | 0.0003 | 0.003 | 0.0081 | 0.0081 | 0.0005 | 0.0006 | 0.0032 | 0.0002 |
Nuclear Periphery | 0.011 | 0.0019 | 0.0047 | 0.0038 | 0.0049 | 0.0027 | 0.0189 | 0.002 | 0.0027 | 0.0098 | 0.0015 | 0.0031 | 0.0286 | 0.0098 | 0.0033 | 0.0114 | 0.016 | 0.0015 |
Nucleolus | 0.0004 | 0.0006 | 0.0003 | 0 | 0.0021 | 0 | 0.0028 | 0.0004 | 0.0004 | 0.0002 | 0 | 0.0008 | 0.0029 | 0.0025 | 0.0003 | 0.0005 | 0.003 | 0 |
Nucleus | 0.0073 | 0.0014 | 0.0022 | 0.0017 | 0.0039 | 0.0014 | 0.0083 | 0.0018 | 0.0014 | 0.0026 | 0.0011 | 0.003 | 0.0176 | 0.0041 | 0.0026 | 0.0042 | 0.0092 | 0.0009 |
Peroxisomes | 0.0002 | 0.0003 | 0.0006 | 0.0055 | 0.0051 | 0.0008 | 0.001 | 0 | 0.0027 | 0.0004 | 0.0001 | 0.0003 | 0.0027 | 0.0001 | 0.0002 | 0.0002 | 0.0201 | 0 |
Punctate Nuclear | 0.0077 | 0.0008 | 0.0007 | 0.0022 | 0.0239 | 0.0007 | 0.016 | 0.0002 | 0.0017 | 0.0144 | 0 | 0.0004 | 0.0032 | 0.0012 | 0.0003 | 0.0004 | 0.001 | 0.0001 |
Vacuole | 0.0195 | 0.0114 | 0.0123 | 0.011 | 0.0108 | 0.0073 | 0.0316 | 0.0081 | 0.0149 | 0.0201 | 0.0107 | 0.0048 | 0.0166 | 0.0052 | 0.0094 | 0.0431 | 0.0094 | 0.0035 |
Vacuole Periphery | 0.0016 | 0.0004 | 0.0024 | 0.0006 | 0.0027 | 0.0009 | 0.0049 | 0.0002 | 0.0022 | 0.0019 | 0.002 | 0.0009 | 0.0031 | 0.0003 | 0.0012 | 0.0043 | 0.0025 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 139.9337 | 122.4467 | 120.5868 | 125.756 | 122.2324 | 125.9128 | 145.3953 | 119.6669 | 129.3729 | 143.1724 |
Translational Efficiency | 1.1491 | 1.3987 | 1.1496 | 1.1834 | 1.2852 | 1.2661 | 1.1009 | 1.3302 | 1.1992 | 1.1988 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1778 | 1134 | 220 | 1178 | 1266 | 1704 | 365 | 1480 | 3044 | 2838 | 585 | 2658 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2164.32 | 2646.80 | 2994.11 | 3592.28 | 2261.00 | 2444.16 | 3500.81 | 4352.16 | 2204.53 | 2525.13 | 3310.26 | 4015.39 |
Standard Deviation | 360.08 | 444.05 | 485.91 | 704.11 | 415.43 | 410.53 | 483.43 | 886.51 | 387.02 | 435.70 | 543.00 | 894.32 |
Intensity Change Log 2 | — | 0.290335 | 0.468213 | 0.730986 | — | 0.112378 | 0.630728 | 0.944771 | — | 0.202156 | 0.553530 | 0.844164 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000704 | 0.001329 | 0.000826 | 0.000712 | 0.001312 | 0.000841 | 0.001672 | 0.000056 | 0.000957 | 0.001036 | 0.001354 | 0.000347 |
Bud Neck | 0.001522 | 0.003494 | 0.012922 | 0.003575 | 0.002179 | 0.001772 | 0.017264 | 0.004739 | 0.001795 | 0.002460 | 0.015631 | 0.004223 |
Bud Site | 0.028454 | 0.032621 | 0.019521 | 0.014308 | 0.023776 | 0.026790 | 0.031550 | 0.015798 | 0.026508 | 0.029120 | 0.027026 | 0.015138 |
Cell Periphery | 0.006531 | 0.010640 | 0.015062 | 0.045923 | 0.010232 | 0.005256 | 0.037403 | 0.000119 | 0.008070 | 0.007407 | 0.029001 | 0.020419 |
Cytoplasm | 0.777763* | 0.637495* | 0.187276* | 0.561513* | 0.684976* | 0.737034* | 0.225664* | 0.687334* | 0.739173* | 0.697260* | 0.211227* | 0.631571* |
Cytoplasmic Foci | 0.029086 | 0.033227 | 0.011697 | 0.005308 | 0.024981 | 0.039796 | 0.011010 | 0.016494 | 0.027379 | 0.037171 | 0.011268 | 0.011537 |
Eisosomes | 0.000008 | 0.000012 | 0.000019 | 0.000017 | 0.000009 | 0.000010 | 0.000030 | 0.000002 | 0.000009 | 0.000011 | 0.000026 | 0.000009 |
Endoplasmic Reticulum | 0.004209 | 0.004220 | 0.002807 | 0.000424 | 0.007244 | 0.003840 | 0.002838 | 0.000104 | 0.005472 | 0.003992 | 0.002826 | 0.000246 |
Endosome | 0.004377 | 0.007056 | 0.011338 | 0.000852 | 0.007039 | 0.006365 | 0.005380 | 0.007011 | 0.005484 | 0.006641 | 0.007621 | 0.004281 |
Golgi | 0.034452 | 0.057924 | 0.017391 | 0.008893 | 0.046576 | 0.044550 | 0.018000 | 0.001944 | 0.039495 | 0.049894 | 0.017771 | 0.005024 |
Lipid Particles | 0.045922 | 0.090614 | 0.074408 | 0.076034 | 0.047634 | 0.058223 | 0.173882* | 0.001086 | 0.046634 | 0.071166 | 0.136473* | 0.034302 |
Mitochondria | 0.000270 | 0.000420 | 0.000022 | 0.000130 | 0.000185 | 0.000398 | 0.000034 | 0.000428 | 0.000235 | 0.000407 | 0.000030 | 0.000296 |
Mitotic Spindle | 0.001063 | 0.005812 | 0.001107 | 0.000506 | 0.001837 | 0.002826 | 0.001252 | 0.001298 | 0.001385 | 0.004019 | 0.001197 | 0.000947 |
None | 0.000548 | 0.000941 | 0.000154 | 0.000722 | 0.000540 | 0.000737 | 0.000208 | 0.000609 | 0.000544 | 0.000818 | 0.000188 | 0.000659 |
Nuclear Periphery | 0.000070 | 0.000211 | 0.000522 | 0.000349 | 0.000147 | 0.000115 | 0.000555 | 0.000038 | 0.000102 | 0.000154 | 0.000542 | 0.000176 |
Nuclear Periphery Foci | 0.007755 | 0.027817 | 0.019835 | 0.009441 | 0.015089 | 0.012006 | 0.023131 | 0.000117 | 0.010805 | 0.018324 | 0.021891 | 0.004249 |
Nucleolus | 0.000107 | 0.000059 | 0.000069 | 0.000091 | 0.000064 | 0.000075 | 0.000079 | 0.000562 | 0.000089 | 0.000069 | 0.000075 | 0.000354 |
Nucleus | 0.001456 | 0.002732 | 0.005340 | 0.010837 | 0.002291 | 0.001923 | 0.005302 | 0.013434 | 0.001803 | 0.002246 | 0.005316 | 0.012283 |
Peroxisomes | 0.000481 | 0.000917 | 0.000288 | 0.000151 | 0.000264 | 0.000450 | 0.000372 | 0.001139 | 0.000391 | 0.000637 | 0.000340 | 0.000702 |
Vacuole | 0.041084 | 0.071411 | 0.609324* | 0.254657* | 0.100979 | 0.045356 | 0.436921* | 0.238948* | 0.065994 | 0.055767 | 0.501756* | 0.245910* |
Vacuole Periphery | 0.014137 | 0.011048 | 0.010072 | 0.005554 | 0.022646 | 0.011637 | 0.007452 | 0.008739 | 0.017676 | 0.011401 | 0.008438 | 0.007328 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.92 | -1.15 | -0.86 | 1.58 | 0.75 | 1.12 | -1.35 | 3.49 | 6.46 | 5.23 | -0.54 | -2.28 | 3.25 | 4.31 | 4.81 |
Bud Neck | -6.74 | -10.35 | -10.20 | -1.71 | 8.22 | 1.88 | -11.51 | -5.45 | -6.57 | 9.23 | -3.98 | -15.14 | -9.31 | -6.86 | 12.05 |
Bud Site | -1.49 | 1.01 | 5.23 | 6.18 | 1.35 | -1.33 | -2.54 | 4.51 | 6.56 | 5.10 | -1.54 | -1.18 | 7.11 | 8.96 | 4.84 |
Cell Periphery | -3.88 | -4.09 | -15.99 | -13.03 | -10.10 | 5.30 | -9.50 | 11.78 | 14.64 | 14.61 | 1.05 | -10.75 | -9.18 | -9.72 | 4.05 |
Cytoplasm | 12.08 | 31.80 | 19.51 | 5.72 | -18.17 | -4.54 | 28.49 | -0.79 | 4.30 | -31.02 | 5.58 | 44.43 | 13.60 | 7.59 | -34.42 |
Cytoplasmic Foci | -1.86 | 5.32 | 14.87 | 13.15 | 3.40 | -6.69 | 6.05 | 3.86 | 10.04 | -2.18 | -6.08 | 8.63 | 11.48 | 15.95 | 0.82 |
Eisosomes | -2.52 | -3.41 | -3.05 | -1.54 | 0.27 | -0.48 | -7.57 | 7.12 | 9.98 | 10.19 | -2.33 | -8.33 | -0.40 | 0.98 | 7.02 |
Endoplasmic Reticulum | 0.01 | 2.22 | 9.03 | 6.58 | 2.78 | 3.65 | 4.02 | 8.35 | 11.47 | 3.02 | 2.76 | 4.22 | 12.13 | 12.39 | 3.94 |
Endosome | -5.52 | -6.36 | 11.92 | 14.77 | 10.09 | 1.12 | 2.93 | 1.23 | 0.25 | -1.45 | -3.19 | -3.58 | 4.26 | 6.83 | 6.31 |
Golgi | -7.12 | 7.93 | 14.66 | 17.02 | 4.22 | 0.63 | 11.85 | 20.31 | 22.36 | 13.09 | -4.96 | 13.98 | 25.47 | 27.49 | 11.84 |
Lipid Particles | -8.16 | -4.12 | -6.65 | 2.75 | 0.32 | -2.57 | -13.45 | 16.27 | 18.99 | 19.85 | -7.51 | -13.43 | 5.09 | 11.95 | 15.64 |
Mitochondria | -0.69 | 7.24 | 2.26 | 1.39 | -1.52 | -2.12 | 4.49 | -2.16 | 0.19 | -4.09 | -1.65 | 8.45 | -0.45 | 1.27 | -4.29 |
Mitotic Spindle | -4.60 | -1.33 | 1.52 | 4.83 | 2.14 | -1.72 | 1.04 | -0.40 | 0.91 | -1.29 | -5.40 | 0.09 | -0.38 | 4.40 | -0.47 |
None | -1.88 | 2.55 | -1.11 | 1.20 | -5.95 | -1.96 | 5.29 | -0.51 | 1.32 | -4.42 | -2.34 | 3.82 | -1.03 | 1.72 | -7.26 |
Nuclear Periphery | -8.55 | -6.75 | -15.13 | -6.06 | 2.75 | 1.74 | -13.21 | 7.05 | 3.29 | 16.38 | -4.46 | -13.78 | -8.92 | -2.91 | 10.79 |
Nuclear Periphery Foci | -11.36 | -6.28 | -2.52 | 10.55 | 5.36 | 2.45 | -4.58 | 16.54 | 13.97 | 17.53 | -7.55 | -8.99 | 10.94 | 15.94 | 15.90 |
Nucleolus | 0.94 | 0.58 | 0.27 | -3.60 | -1.90 | -1.05 | -2.49 | -15.86 | -15.00 | -15.20 | 0.66 | 0.27 | -8.84 | -14.88 | -14.80 |
Nucleus | -5.25 | -3.17 | -12.23 | -10.60 | -2.04 | 1.83 | -3.82 | -16.16 | -16.58 | -6.72 | -3.23 | -5.12 | -20.39 | -19.64 | -6.25 |
Peroxisomes | -0.75 | 1.42 | 5.41 | 1.29 | 1.00 | -3.17 | -0.80 | -6.21 | -4.59 | -4.34 | -1.06 | 0.62 | -3.78 | -0.30 | -3.21 |
Vacuole | -7.21 | -26.98 | -30.49 | -23.77 | 15.23 | 10.68 | -23.21 | -16.34 | -27.38 | 13.67 | 3.01 | -35.55 | -34.01 | -36.55 | 19.57 |
Vacuole Periphery | 2.40 | 3.82 | 7.61 | 5.28 | 3.19 | 6.17 | 9.17 | 9.32 | 4.87 | -0.25 | 5.93 | 9.69 | 11.82 | 7.19 | 1.98 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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