Standard name
Human Ortholog
Description One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.05 0.05 0.06 0.06 0.08 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.92 0.89 0.91 0.88 0.8 0.81 0.81 0.8 0.83 0.82 0.97 0.97 0.97 0.95 0.98 0.97 0.91 0.95 0.87 0.91 0.92 0.9
Endoplasmic Reticulum 0.05 0.05 0.09 0.1 0.09 0.12 0.16 0.14 0.16 0.15 0.12 0.15 0 0 0 0 0.06 0 0 0 0.1 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 4 4 8 21 27 22 29 13 15 0 0 0 0 0 1 0 1 0 0 3 2
Bud 0 4 4 5 7 11 25 25 18 25 12 8 3 4 4 2 0 0 1 0 1 2 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 5 20 10 12 37 46 46 54 53 36 34 27 23 13 4 14 2 0 0 3 0 0 0
Cytoplasm 157 341 516 394 545 697 736 736 701 770 378 499 557 715 653 328 393 335 162 329 491 380 559 509
Endoplasmic Reticulum 8 16 49 45 54 91 146 131 142 148 54 92 24 25 23 4 23 6 5 8 56 7 16 23
Endosome 0 0 0 0 0 1 0 0 2 1 0 0 0 0 1 0 0 0 0 0 0 4 5 7
Golgi 0 1 6 10 7 5 5 4 4 5 3 0 1 0 0 5 1 1 0 0 0 0 0 0
Mitochondria 0 0 0 2 0 2 13 13 5 7 10 7 0 0 0 6 0 2 0 0 3 0 0 0
Nucleus 0 1 0 1 0 0 0 0 0 1 0 1 0 2 1 1 2 0 0 1 0 5 2 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 1 0 0 1 3 1
Nucleolus 0 0 0 0 1 0 1 1 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Vac/Vac Membrane 0 0 0 2 4 1 7 6 5 6 3 6 1 1 2 6 5 8 0 1 2 13 6 7
Unique Cell Count 164 350 562 445 597 790 918 910 870 961 457 612 576 735 671 344 402 344 178 346 567 420 610 564
Labelled Cell Count 169 368 596 473 634 853 1000 989 953 1051 509 663 613 770 697 357 438 355 178 346 567 420 610 564


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.1 36.5 34.7 29.6 29.2 29.0 27.1 27.1 27.8 27.7 25.3 25.6 38.1 39.4 36.5 39.7 41.9 39.7 33.3 36.0 37.5
Std Deviation (1e-4) 6.8 6.5 7.7 5.9 5.2 5.0 5.3 5.3 5.4 5.9 6.2 6.1 7.7 7.5 7.3 14.0 12.0 13.7 7.3 8.1 8.6
Intensity Change (Log2) – – – -0.23 -0.25 -0.26 -0.35 -0.35 -0.32 -0.32 -0.46 -0.44 0.14 0.18 0.07 0.19 0.27 0.19 -0.06 0.05 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.3 3.8 3.5 3.8 3.6 3.4 0 0 0 0 0 0
Bud 0 0.8 1.2 2.7 2.7 2.0 2.6 2.4 1.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 -1.6 1.0 1.3 1.3 2.2 1.7 3.0 1.6 1.0 -0.4 -1.8 -2.2 -0.1 -2.8
Cytoplasm -1.7 -0.3 -2.1 -6.0 -5.7 -5.8 -6.1 -4.4 -5.1 3.6 4.4 4.3 2.0 3.9 3.4
Endoplasmic Reticulum 0.8 0.2 1.7 4.0 3.2 4.1 3.7 1.6 3.3 -3.1 -4.1 -3.9 -4.7 -1.7 -4.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.5 0.2 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 36.8377 41.5941 40.5871 38.6696 37.6732 40.7307 34.1084 46.49 43.1468 41.8955 40.9014 42.3718 38.3383 44.2062 42.7874 39.5121 37.2823 41.7879
Actin 0.0023 0.004 0.0064 0.0323 0.0093 0.0027 0.0096 0.0004 0.0058 0.0093 0.0002 0.0042 0.0072 0.002 0.0011 0.0107 0.0234 0.0005
Bud 0.0005 0.0002 0.0012 0.0002 0.0013 0.0002 0.0012 0.0004 0.0004 0.0002 0.0003 0.0003 0.0004 0.0004 0.0008 0.0014 0.0019 0.0001
Bud Neck 0.0006 0.0005 0.0007 0.0017 0.0012 0.0102 0.003 0.0008 0.0019 0.0008 0.001 0.0101 0.0019 0.0019 0.0011 0.0013 0.0041 0.0066
Bud Periphery 0.0003 0.0001 0.0005 0.0002 0.0012 0.0001 0.0014 0.0001 0.0004 0.0001 0.0003 0.0005 0.0004 0.0001 0.0006 0.0008 0.0033 0
Bud Site 0.0008 0.0005 0.0005 0.0002 0.0074 0.0001 0.0046 0.0092 0.0009 0.0003 0 0.0002 0.001 0.0034 0.0003 0.0007 0.0049 0
Cell Periphery 0.0015 0.0019 0.0009 0.0014 0.0002 0.0015 0.0076 0.0035 0.0009 0.0003 0.0002 0.0007 0.0055 0.0032 0.0008 0.0021 0.0038 0.0004
Cytoplasm 0.8483 0.9379 0.9324 0.9011 0.8715 0.9047 0.767 0.9399 0.9128 0.9025 0.9519 0.9067 0.8256 0.925 0.9378 0.8725 0.8381 0.9185
Cytoplasmic Foci 0.0117 0.0088 0.0041 0.0069 0.0095 0.0033 0.0142 0.005 0.0113 0.0074 0.0048 0.003 0.0057 0.0032 0.0031 0.0069 0.0054 0.0035
Eisosomes 0 0.0003 0 0.0001 0.0002 0 0.0003 0 0.0001 0 0 0.0002 0.0008 0.0028 0 0.0001 0.0008 0
Endoplasmic Reticulum 0.069 0.0158 0.0214 0.0197 0.004 0.0543 0.0506 0.0186 0.0153 0.0199 0.0095 0.0428 0.0493 0.0219 0.0261 0.0133 0.0039 0.0606
Endosome 0.0095 0.0025 0.0059 0.0045 0.0249 0.0051 0.021 0.0015 0.0073 0.0046 0.0142 0.0053 0.0089 0.002 0.0056 0.0093 0.0071 0.0027
Golgi 0.0015 0.0008 0.0005 0.0021 0.0046 0.0015 0.0039 0.0002 0.002 0.0008 0.0008 0.0016 0.0016 0.0004 0.0003 0.0017 0.0053 0.0003
Lipid Particles 0.0033 0.0058 0.0008 0.0019 0.0073 0.0018 0.0176 0.0075 0.0044 0.0026 0.0002 0.0011 0.0069 0.0023 0.0006 0.002 0.0155 0.0001
Mitochondria 0.0009 0.0007 0.0008 0.0005 0.0021 0.0004 0.009 0 0.0066 0.0003 0.0009 0.0067 0.0019 0.0002 0.0041 0.0124 0.018 0.0001
None 0.0018 0.0036 0.0007 0.002 0.0019 0.0002 0.0057 0.0002 0.004 0.0013 0.0003 0.003 0.0081 0.0081 0.0005 0.0006 0.0032 0.0002
Nuclear Periphery 0.011 0.0019 0.0047 0.0038 0.0049 0.0027 0.0189 0.002 0.0027 0.0098 0.0015 0.0031 0.0286 0.0098 0.0033 0.0114 0.016 0.0015
Nucleolus 0.0004 0.0006 0.0003 0 0.0021 0 0.0028 0.0004 0.0004 0.0002 0 0.0008 0.0029 0.0025 0.0003 0.0005 0.003 0
Nucleus 0.0073 0.0014 0.0022 0.0017 0.0039 0.0014 0.0083 0.0018 0.0014 0.0026 0.0011 0.003 0.0176 0.0041 0.0026 0.0042 0.0092 0.0009
Peroxisomes 0.0002 0.0003 0.0006 0.0055 0.0051 0.0008 0.001 0 0.0027 0.0004 0.0001 0.0003 0.0027 0.0001 0.0002 0.0002 0.0201 0
Punctate Nuclear 0.0077 0.0008 0.0007 0.0022 0.0239 0.0007 0.016 0.0002 0.0017 0.0144 0 0.0004 0.0032 0.0012 0.0003 0.0004 0.001 0.0001
Vacuole 0.0195 0.0114 0.0123 0.011 0.0108 0.0073 0.0316 0.0081 0.0149 0.0201 0.0107 0.0048 0.0166 0.0052 0.0094 0.0431 0.0094 0.0035
Vacuole Periphery 0.0016 0.0004 0.0024 0.0006 0.0027 0.0009 0.0049 0.0002 0.0022 0.0019 0.002 0.0009 0.0031 0.0003 0.0012 0.0043 0.0025 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 139.9337 122.4467 120.5868 125.756 122.2324 125.9128 145.3953 119.6669 129.3729 143.1724
Translational Efficiency 1.1491 1.3987 1.1496 1.1834 1.2852 1.2661 1.1009 1.3302 1.1992 1.1988

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Kes1

Kes1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kes1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available