ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0
Bud – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 – 0.26 – 0.23 0.33 0.28 0.33 – 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 – 0.05 – 0.05 0 0.07 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 – 0 – 0 0 0 0 – 0 0.06 0.05 0 0 0 0.05 0 0 0.05 0.08 0.07
Golgi – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0.26 0.12 – 0.18 – 0.24 0.24 0.18 0.28 – 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleus – 0.13 0.08 0 – 0.07 – 0 0 0 0 – 0.09 0.06 0 0.16 0.14 0.09 0 0.08 0 0.08 0.07 0.07
Nuclear Periphery – 0 0.15 0.21 – 0.08 – 0.05 0.08 0.06 0 – 0 0.1 0.09 0.12 0.12 0.15 0.1 0 0 0 0 0
Nucleolus – 0 0 0.06 – 0 – 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.87 0.87 0.85 – 0.58 – 0.55 0.49 0.53 0.49 – 0.85 0.89 0.9 0.8 0.8 0.82 0.6 0.86 0.86 0.79 0.75 0.74
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 – 0 – 2 4 2 0 – 0 0 0 0 0 0 3 0 0 0 0 0
Bud – 0 0 0 – 2 – 4 6 6 4 – 0 0 1 0 0 0 0 0 0 1 3 2
Bud Neck – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 1 – 4 – 5 6 7 6 – 0 0 0 2 3 0 0 0 0 0 0 0
Cytoplasm – 7 1 10 – 133 – 132 152 194 189 – 9 5 12 8 6 7 0 1 0 1 1 1
Endoplasmic Reticulum – 0 0 1 – 25 – 31 19 48 26 – 0 0 3 6 2 3 0 0 0 0 3 4
Endosome – 1 1 11 – 4 – 7 2 13 2 – 7 15 13 1 0 2 2 2 7 10 29 20
Golgi – 2 1 0 – 0 – 0 1 2 0 – 0 1 1 3 3 4 0 0 0 1 3 1
Mitochondria – 4 20 39 – 93 – 141 110 123 160 – 5 3 2 3 2 2 2 1 4 2 4 6
Nucleus – 26 6 6 – 33 – 21 7 16 12 – 21 17 10 22 18 15 1 17 10 15 27 21
Nuclear Periphery – 2 12 71 – 39 – 28 35 42 16 – 10 26 24 17 16 26 4 2 12 6 11 8
Nucleolus – 5 1 19 – 2 – 6 3 5 4 – 8 15 10 4 3 6 1 1 2 1 6 5
Peroxisomes – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 1 – 1 – 0 0 1 0 – 1 0 1 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane – 170 68 283 – 291 – 318 226 364 283 – 193 239 251 111 102 137 28 178 262 164 286 222
Unique Cell Count 195 78 333 506 578 464 682 575 226 268 278 138 128 168 47 207 305 208 381 300
Labelled Cell Count 217 110 442 627 695 571 823 702 254 321 328 177 155 202 47 207 305 208 381 300


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 13.8 8.6 8.0 – 6.9 – 6.4 6.5 6.8 6.6 – 11.5 10.4 11.0 15.2 16.8 17.1 13.3 12.4 12.2
Std Deviation (1e-4) – 3.9 2.6 2.2 – 2.0 – 1.7 1.7 1.7 1.6 – 3.3 2.8 3.1 5.9 6.0 5.3 4.3 3.8 3.8
Intensity Change (Log2) – – – -0.09 – -0.32 – -0.41 -0.41 -0.33 -0.39 – 0.42 0.28 0.36 0.83 0.97 0.99 0.64 0.53 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 – 4.9 – 4.4 5.7 5.2 5.8 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Mitochondria -3.2 – -1.5 – -0.2 -0.4 -1.6 0.4 – -6.5 -7.7 0 -5.4 -5.4 -6.3
Nucleus 0 – -0.4 – 0 0 0 0 – 0.4 -0.4 0 1.7 1.4 0.3
Nuclear Periphery 1.2 – -2.2 – 0 -2.3 -3.0 0 – -3.2 -1.4 -1.7 -0.6 -0.6 0
Nucleolus 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Vacuole -0.5 – -5.0 – -5.4 -6.3 -5.7 -6.3 – -0.4 0.5 0.8 -1.3 -1.4 -1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.465 7.6739 6.2347 6.1634 5.0129 6.0766 7.8345 9.8147 8.621 7.4616 7.5909 8.5835 5.6254 7.6276 6.2274 6.2453 5.536 5.9669
Actin 0.0094 0 0.0041 0.0163 0.0134 0.0051 0.0032 0 0 0 0 0 0.0005 0 0 0 0 0
Bud 0.0003 0.0002 0.0002 0.0001 0.001 0.0002 0.0003 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0
Bud Neck 0.0014 0 0.0006 0.0002 0.0004 0.0006 0.0011 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001
Bud Periphery 0.0007 0.0004 0.0005 0.0002 0.0015 0.0006 0.001 0.0001 0.0003 0.0001 0 0.0003 0.0003 0.0002 0.0008 0.0006 0.0003 0.0002
Bud Site 0.0014 0.0002 0.0009 0.0002 0.0022 0.0005 0.0004 0 0.0001 0 0 0 0.0002 0.0001 0.0002 0 0 0
Cell Periphery 0.0015 0.001 0.0017 0.0007 0.0009 0.0008 0.0013 0.0006 0.0012 0.0004 0.0003 0.0005 0.0019 0.0013 0.0022 0.0006 0.0004 0.0007
Cytoplasm 0.0036 0.0015 0.0015 0.0002 0.003 0.0006 0.0005 0.0006 0.0006 0.0001 0 0.0001 0.0003 0.0011 0.0003 0.0001 0.0002 0.0001
Cytoplasmic Foci 0.0112 0.0003 0.0074 0.0025 0.0096 0.0027 0.0014 0.0001 0.0003 0.0001 0 0 0.0012 0.0001 0.0003 0.0001 0.0002 0.0001
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0037 0.0003 0.0012 0.0002 0.0021 0.0021 0.0006 0.0008 0.0006 0.0002 0.0001 0.0004 0.0003 0.0004 0.0005 0.0001 0.0001 0.0003
Endosome 0.0459 0.0116 0.0351 0.0167 0.0496 0.0191 0.0269 0.0122 0.0208 0.0163 0.0051 0.0082 0.0193 0.0088 0.0169 0.0123 0.016 0.0104
Golgi 0.0067 0.0003 0.004 0.0047 0.008 0.0032 0.0013 0.0001 0.0008 0.0006 0 0.0001 0.0007 0.0001 0.0005 0.0002 0.0003 0.0001
Lipid Particles 0.0142 0.0014 0.0087 0.002 0.015 0.0044 0.0041 0.0003 0.0045 0.0021 0.0001 0.0009 0.0075 0.0006 0.0036 0.0006 0.0026 0.0008
Mitochondria 0.0154 0.0016 0.0178 0.0013 0.023 0.01 0.0098 0.0005 0.0265 0.0073 0.0001 0.0059 0.0088 0.0007 0.0063 0.0027 0.0006 0.0008
None 0.0027 0 0.0014 0 0.0008 0.0003 0.0006 0 0.0002 0 0 0 0.0002 0.0003 0 0 0 0
Nuclear Periphery 0.0141 0.0163 0.0078 0.0018 0.006 0.0099 0.0071 0.0202 0.0152 0.0011 0.0022 0.0062 0.0053 0.004 0.008 0.0008 0.0013 0.0094
Nucleolus 0.0065 0.0008 0.0102 0.0016 0.0016 0.0018 0.0028 0.0002 0.0043 0.0003 0.0003 0.0004 0.0046 0.0025 0.0054 0.0004 0.0005 0.0023
Nucleus 0.0062 0.002 0.0049 0.001 0.0047 0.006 0.0047 0.0004 0.0031 0.0003 0.0005 0.0007 0.0069 0.0041 0.0045 0.0006 0.0005 0.0016
Peroxisomes 0.0059 0 0.0035 0.006 0.0065 0.0013 0.0006 0 0.0005 0.0001 0 0 0.0023 0 0.0002 0.0001 0.0001 0
Punctate Nuclear 0.0025 0 0.0006 0.0001 0.0026 0.0027 0.0006 0 0 0 0 0 0.0002 0 0 0 0 0
Vacuole 0.3629 0.3761 0.3505 0.4049 0.3545 0.3109 0.338 0.2868 0.3151 0.3082 0.2399 0.2431 0.4083 0.3645 0.4102 0.4896 0.3554 0.2943
Vacuole Periphery 0.4835 0.586 0.5373 0.5393 0.4937 0.6173 0.5938 0.677 0.6057 0.6628 0.7512 0.7329 0.5309 0.6111 0.5398 0.4909 0.6213 0.6788

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.3829 7.1998 16.9182 14.7732 7.8318 16.0992 16.6827 17.6061 13.251 12.5603
Translational Efficiency 0.8261 1.6465 0.7547 0.8971 1.0267 0.3467 0.5801 0.7435 0.8923 0.7287

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
81 461 1244 19 180 1526 2601 628 261 1987 3845 647

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 868.44 1136.13 948.49 323.49 905.50 1051.43 1027.78 1014.65 894.00 1071.08 1002.13 994.35
Standard Deviation 93.09 265.93 179.36 5.01 166.92 225.61 193.54 202.88 148.99 238.28 192.67 231.45
Intensity Change Log 2 — 0.387630 0.127206 -1.424705 — 0.215566 0.182745 0.164196 — 0.302365 0.155823 -0.406728

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000022 0.000156 0.000055 0.000084 0.000021 0.000026 0.000018 0.000136 0.000021 0.000056 0.000030 0.000134
Bud Neck 0.005612 0.002860 0.011856 0.000008 0.001701 0.000142 0.000915 0.002779 0.002915 0.000773 0.004455 0.002698
Bud Site 0.008330 0.011919 0.004793 0.000032 0.008504 0.002273 0.003135 0.009570 0.008450 0.004511 0.003671 0.009290
Cell Periphery 0.000200 0.000645 0.001646 0.000718 0.000754 0.001721 0.000665 0.000408 0.000582 0.001471 0.000982 0.000417
Cytoplasm 0.031248 0.002928 0.009643 0.000161 0.005036 0.001397 0.000782 0.006144 0.013171 0.001752 0.003649 0.005968
Cytoplasmic Foci 0.025489 0.003369 0.002758 0.000008 0.005405 0.000728 0.000488 0.010044 0.011638 0.001341 0.001223 0.009750
Eisosomes 0.000018 0.000018 0.000066 0.000626 0.000008 0.000003 0.000008 0.000016 0.000011 0.000007 0.000027 0.000034
Endoplasmic Reticulum 0.004935 0.000719 0.003741 0.000002 0.001039 0.000938 0.000885 0.001398 0.002248 0.000887 0.001809 0.001357
Endosome 0.080451 0.023484 0.013666 0.000000 0.022010 0.003298 0.005245 0.045317 0.040147 0.007981 0.007969 0.043986
Golgi 0.000765 0.006413 0.001280 0.000004 0.002462 0.001760 0.001497 0.011962 0.001935 0.002840 0.001427 0.011611
Lipid Particles 0.001139 0.000534 0.001001 0.000020 0.000657 0.000172 0.000252 0.001012 0.000806 0.000256 0.000494 0.000983
Mitochondria 0.000651 0.009846 0.003087 0.000019 0.002042 0.009841 0.003327 0.013482 0.001610 0.009842 0.003249 0.013086
Mitotic Spindle 0.000180 0.000585 0.001153 0.000159 0.000143 0.000359 0.000316 0.000411 0.000154 0.000412 0.000587 0.000404
None 0.000029 0.000462 0.000650 0.997885* 0.000124 0.000224 0.000148 0.000360 0.000095 0.000279 0.000310 0.029654
Nuclear Periphery 0.000481 0.000393 0.001749 0.000000 0.000589 0.000263 0.001363 0.001461 0.000556 0.000293 0.001488 0.001418
Nuclear Periphery Foci 0.000162 0.000404 0.000431 0.000001 0.000261 0.000124 0.000392 0.001405 0.000230 0.000189 0.000404 0.001364
Nucleolus 0.000328 0.003309 0.007133 0.000003 0.000456 0.000187 0.001345 0.003081 0.000417 0.000911 0.003217 0.002991
Nucleus 0.006667 0.003519 0.042948 0.000008 0.003388 0.000171 0.001557 0.004915 0.004406 0.000948 0.014949 0.004771
Peroxisomes 0.000517 0.000764 0.000626 0.000260 0.000322 0.000063 0.000073 0.001465 0.000382 0.000225 0.000252 0.001429
Vacuole 0.597927* 0.433224* 0.390008* 0.000001 0.454672* 0.131524 0.330549* 0.300119* 0.499130* 0.201521* 0.349786* 0.291305*
Vacuole Periphery 0.234849* 0.494452* 0.501714* 0.000000 0.490406* 0.844784* 0.647041* 0.584516* 0.411095* 0.763505* 0.600022* 0.567351*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.26 -2.25 -1.50 0.64 -0.60 -0.49 0.24 -4.04 -3.91 -4.25 -1.34 -1.09 -4.15 -2.15 -3.82
Bud Neck 1.45 -2.59 3.13 4.61 7.31 2.44 1.18 -1.34 -5.28 -3.61 2.93 -1.74 0.25 -3.79 2.46
Bud Site -0.85 1.11 2.58 5.96 7.14 1.49 1.28 -0.25 -4.24 -3.73 1.30 1.58 -0.23 -2.80 -3.39
Cell Periphery -4.09 -3.60 -0.94 -0.13 1.37 -3.35 0.34 1.60 6.22 1.55 -4.16 -1.81 1.07 6.25 3.20
Cytoplasm 2.65 2.00 2.90 3.45 5.59 1.68 1.67 -0.39 -2.91 -2.89 3.07 2.42 1.87 -2.68 -1.03
Cytoplasmic Foci 2.55 2.62 2.94 4.41 3.81 2.78 2.93 -2.01 -6.19 -6.36 3.45 3.48 0.65 -5.72 -5.77
Eisosomes -0.02 -3.79 -1.91 -1.91 -1.76 1.88 0.03 -2.33 -6.74 -3.94 1.67 -3.43 -2.16 -2.73 -0.67
Endoplasmic Reticulum 2.30 0.63 2.70 7.47 9.95 0.61 1.04 -1.76 -2.56 -3.14 2.31 0.72 1.46 -2.81 2.24
Endosome 3.89 4.69 5.68 6.67 10.34 6.22 5.56 -4.28 -9.18 -8.75 6.20 6.26 -0.59 -7.96 -8.06
Golgi -5.32 -2.15 4.02 6.14 8.53 0.71 0.99 -5.08 -6.22 -6.42 -1.23 0.75 -5.70 -5.47 -6.46
Lipid Particles 1.37 0.36 3.27 1.88 6.61 1.23 1.04 -0.67 -3.39 -3.07 1.81 1.07 -0.42 -2.93 -2.02
Mitochondria -3.34 -4.65 3.21 3.58 6.31 -4.78 -1.55 -5.66 -1.50 -5.12 -5.92 -2.75 -6.00 -1.42 -5.18
Mitotic Spindle -1.74 -1.59 0.38 2.64 1.73 -0.71 -1.81 -3.26 -0.13 -0.75 -1.01 -2.11 -2.85 0.07 0.98
None -7.09 -3.17 -1004.31* -1002.22* -985.04* -1.51 -0.36 -2.87 -2.46 -3.86 -3.91 -2.81 -4.46 -4.43 -4.43
Nuclear Periphery 0.39 -2.89 2.45 3.68 4.45 0.76 -1.37 -1.70 -4.19 -0.21 0.87 -2.27 -2.11 -4.05 0.18
Nuclear Periphery Foci -1.99 -2.91 4.12 3.56 5.06 1.26 -0.95 -2.63 -3.03 -2.37 0.54 -1.81 -2.72 -2.86 -2.32
Nucleolus -3.45 -6.39 1.74 3.91 6.79 1.52 -3.62 -3.22 -3.66 -2.15 -2.01 -7.26 -3.29 -2.61 0.28
Nucleus 0.75 -5.84 1.55 4.40 10.26 1.30 0.70 -0.44 -3.91 -2.58 1.61 -3.86 -0.04 -3.21 5.63
Peroxisomes -0.75 -0.60 1.60 1.50 1.89 2.46 2.37 -3.59 -4.68 -4.65 1.43 1.36 -3.47 -4.01 -3.98
Vacuole 5.26 6.80 20.61 40.86* 46.85* 16.77 6.38 7.47 -18.02 3.10 17.47 8.82 11.29 -9.25 6.20
Vacuole Periphery -9.05 -9.46 8.86 42.38* 50.09* -16.96 -7.44 -4.01 20.84 4.93 -18.89 -10.20 -7.30 14.98 2.51
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
Localization
Cell Percentages vacuolar membrane (67%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

YPL162C

YPL162C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl162c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available