Standard name
Human Ortholog
Description Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0.07 0 – 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.58 0.57 – 0.65 0.44 0.61 0.57 – 0.57 0.48 0.5 0.53 0.48 0.56 0.61 0.63 0.45 0.5 0.46 0.47 0.63 0.61 0.63 0.54
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0 – 0.15 0.11 0.09 0.2 – 0.12 0.11 0.05 0.13 0 0.09 0.16 0 0 0.07 0.07 0 0 0 0.06 0.1
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.09 – 0 0 0.21 0 – 0.26 0.36 0.36 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0.12 – 0 0 0 0 – 0 0.05 0.08 0.07 0.09 0 0 0.14 0.06 0 0.17 0.23 0.17 0.15 0.09 0.08
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0.07 0 0.07 – 0 0.08 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0.42 0.35 – 0.26 0.44 0.13 0.23 – 0.14 0.1 0.08 0.06 0.46 0.51 0.43 0.41 0.58 0.55 0.16 0.18 0.12 0.11 0.09 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 – 0 2 1 0 0 0 0 0 0 0 3 1 0 2 3 2
Bud 0 0 – 0 0 5 0 – 3 4 9 6 0 1 1 0 0 0 1 2 0 1 4 5
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 3
Bud Site 0 0 – 0 0 1 0 – 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 1 0 0 – 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 88 81 – 43 24 46 84 – 78 70 63 79 48 137 157 89 46 52 87 84 20 99 177 111
Endoplasmic Reticulum 0 4 – 0 0 0 1 – 0 1 0 0 1 1 2 6 0 1 0 0 0 1 4 1
Endosome 9 0 – 10 6 7 29 – 16 16 6 19 4 22 41 4 2 7 13 4 0 3 15 20
Golgi 1 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 1 1 2
Mitochondria 8 12 – 2 0 16 6 – 36 53 45 44 4 0 1 0 2 2 4 2 0 2 5 7
Nucleus 13 17 – 2 2 0 2 – 4 7 10 10 9 3 9 20 6 2 32 41 5 23 25 17
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 2 1 0 1 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 – 0 0 1 0 – 1 1 1 2 0 3 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 1 0 1 2 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 1 – 2 4 3 11 – 6 11 4 1 0 5 7 0 0 0 9 5 0 3 7 8
Vac/Vac Membrane 65 49 – 17 24 10 34 – 20 14 10 9 46 125 111 58 60 58 29 31 3 17 26 17
Unique Cell Count 153 141 66 54 75 148 138 146 126 150 99 245 256 141 103 105 189 180 32 162 282 207
Labelled Cell Count 185 164 77 61 90 170 165 180 151 173 113 297 330 177 118 123 189 180 32 162 282 207


Cytoplasm, Nucleus, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.6 – 5.6 6.6 4.8 5.9 – 4.9 5.0 5.0 5.2 5.9 6.8 7.1 8.8 9.4 9.2 5.2 5.5 6.4
Std Deviation (1e-4) 0.7 0.8 – 1.2 1.1 2.2 1.5 – 1.0 1.3 1.8 1.5 1.4 1.4 1.3 1.5 1.6 1.5 1.1 1.5 2.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2911 0.1351 -0.417 -0.6813 -0.9217 -0.2526 1.5208 1.4555 1.1328 0.8555 0.8862 1.209 2.7451 3.649 3.064 3.269 2.6938 3.2206
Actin 0.0257 0.0002 0.004 0.0037 0.0057 0.0051 0.0155 0.0006 0.016 0.0012 0.0034 0.0149 0.0085 0.0004 0.0087 0.0079 0.0029 0.0058
Bud 0.0024 0.0001 0.002 0.0021 0.0003 0.0015 0.0008 0.001 0.0004 0.0003 0.0003 0.0004 0.0004 0.0002 0.0006 0.0001 0.0002 0.0002
Bud Neck 0.0049 0.0006 0.0013 0.0007 0.001 0.0027 0.0031 0.0006 0.001 0.0009 0.001 0.0051 0.0022 0.0005 0.001 0.0011 0.002 0.0036
Bud Periphery 0.005 0 0.0007 0.0006 0.0006 0.0008 0.0008 0.0005 0.0003 0.0002 0.0003 0.0004 0.0006 0.0001 0.0006 0.0001 0.0001 0.0001
Bud Site 0.0047 0.0013 0.0027 0.0004 0.0007 0.0003 0.0067 0.0099 0.0066 0.0012 0.0007 0.0011 0.001 0.0011 0.0017 0.0003 0.0004 0.0002
Cell Periphery 0.0004 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0
Cytoplasm 0.1729 0.3246 0.2041 0.1965 0.2128 0.1995 0.3055 0.442 0.3117 0.2824 0.2305 0.2548 0.256 0.2403 0.2244 0.1984 0.2749 0.2204
Cytoplasmic Foci 0.0218 0.0175 0.0186 0.0389 0.01 0.0201 0.0477 0.0377 0.0141 0.03 0.0259 0.0174 0.01 0.0112 0.0133 0.0124 0.0197 0.0206
Eisosomes 0.0004 0 0.0001 0.0003 0.0002 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0101 0.0059 0.0048 0.0035 0.0032 0.0043 0.0103 0.0069 0.0049 0.0034 0.0022 0.0028 0.0056 0.0029 0.0047 0.0031 0.0068 0.0034
Endosome 0.0362 0.0182 0.015 0.0109 0.029 0.0144 0.0593 0.0123 0.0116 0.0293 0.0409 0.0101 0.0127 0.0029 0.0121 0.0058 0.0432 0.0128
Golgi 0.0059 0.0004 0.0022 0.0055 0.0173 0.0031 0.0115 0.0007 0.004 0.0064 0.0377 0.0047 0.0016 0.0002 0.0028 0.0012 0.001 0.0012
Lipid Particles 0.0057 0.0007 0.0037 0.0086 0.0354 0.0047 0.0193 0.0004 0.0044 0.0018 0.0292 0.0024 0.0049 0.0001 0.0039 0.0016 0.0027 0.001
Mitochondria 0.0037 0.0005 0.0074 0.0035 0.021 0.0052 0.0064 0.0006 0.0013 0.0032 0.0774 0.0087 0.0013 0.0006 0.0027 0.0008 0.0013 0.0007
None 0.0514 0.1452 0.1278 0.2066 0.0629 0.093 0.0836 0.1117 0.1382 0.0417 0.1514 0.0552 0.1179 0.1883 0.1682 0.1308 0.0712 0.0875
Nuclear Periphery 0.0483 0.0249 0.0332 0.0182 0.0397 0.0218 0.0475 0.0159 0.0162 0.0154 0.0118 0.0134 0.0241 0.0155 0.0143 0.0139 0.0198 0.0096
Nucleolus 0.005 0.0027 0.0032 0.0023 0.0043 0.0034 0.0025 0.0011 0.001 0.0011 0.0043 0.0028 0.0019 0.0018 0.0026 0.0015 0.0021 0.0029
Nucleus 0.5339 0.4355 0.532 0.4395 0.4798 0.5643 0.3186 0.342 0.4324 0.5479 0.3279 0.555 0.5237 0.512 0.515 0.5938 0.5054 0.5853
Peroxisomes 0.0087 0.0004 0.0051 0.0204 0.0082 0.0017 0.0245 0.0011 0.0134 0.0056 0.0192 0.0169 0.002 0.001 0.0029 0.0037 0.0022 0.0056
Punctate Nuclear 0.0424 0.0172 0.0284 0.036 0.0619 0.0507 0.0257 0.0115 0.019 0.0254 0.0332 0.0302 0.0199 0.0189 0.017 0.0216 0.0398 0.0369
Vacuole 0.0078 0.0035 0.0027 0.0012 0.0027 0.0023 0.0075 0.003 0.0027 0.0019 0.0019 0.0031 0.0045 0.0017 0.0026 0.0016 0.0034 0.0017
Vacuole Periphery 0.0028 0.0006 0.0009 0.0005 0.0032 0.001 0.0026 0.0004 0.0005 0.0007 0.0008 0.0005 0.001 0.0003 0.0007 0.0003 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
355 519 273 1829 2064 2157 2276 1179 2419 2676 2549 3008

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 739.72 806.13 1046.57 803.87 754.90 810.45 870.75 872.58 752.67 809.61 889.58 830.80
Standard Deviation 112.54 96.66 116.07 104.19 85.15 95.38 96.38 110.37 89.85 95.65 112.66 111.80
Intensity Change Log 2 — 0.124033 0.500618 0.119983 — 0.102438 0.205973 0.209002 — 0.113166 0.359312 0.165631

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000359 0.000393 0.001292 0.000329 0.000080 0.000163 0.000078 0.000469 0.000121 0.000207 0.000208 0.000384
Bud Neck 0.014299 0.011294 0.007603 0.014758 0.007139 0.019766 0.021891 0.025215 0.008190 0.018123 0.020360 0.018857
Bud Site 0.010867 0.005515 0.011768 0.010937 0.001715 0.009771 0.006611 0.012479 0.003058 0.008946 0.007163 0.011541
Cell Periphery 0.000391 0.000168 0.000207 0.000146 0.000084 0.000085 0.000070 0.000208 0.000129 0.000101 0.000085 0.000170
Cytoplasm 0.415787* 0.390952* 0.206694* 0.330135* 0.411750* 0.395616* 0.353111* 0.286196* 0.412342* 0.394711* 0.337430* 0.312913*
Cytoplasmic Foci 0.214329* 0.135727 0.019258 0.048041 0.065837 0.046646 0.006180 0.078160 0.087629 0.063923 0.007581 0.059847
Eisosomes 0.000192 0.000075 0.000067 0.000046 0.000037 0.000028 0.000009 0.000077 0.000060 0.000037 0.000015 0.000058
Endoplasmic Reticulum 0.004286 0.002604 0.012301 0.002934 0.000754 0.000771 0.000681 0.003998 0.001272 0.001126 0.001925 0.003351
Endosome 0.018191 0.011091 0.009303 0.018530 0.004433 0.004353 0.000948 0.036377 0.006452 0.005659 0.001843 0.025525
Golgi 0.001607 0.002099 0.000231 0.000673 0.000758 0.000635 0.000058 0.000770 0.000883 0.000919 0.000076 0.000711
Lipid Particles 0.017004 0.007418 0.002632 0.002570 0.003276 0.002337 0.000425 0.004910 0.005291 0.003323 0.000661 0.003487
Mitochondria 0.004083 0.003740 0.000817 0.001118 0.001432 0.004900 0.001492 0.001446 0.001821 0.004675 0.001420 0.001247
Mitotic Spindle 0.003084 0.002418 0.009268 0.010020 0.005839 0.008101 0.009018 0.008141 0.005434 0.006999 0.009045 0.009283
None 0.007434 0.009396 0.006671 0.008492 0.005986 0.005751 0.005001 0.006255 0.006198 0.006458 0.005180 0.007616
Nuclear Periphery 0.002736 0.001144 0.002234 0.002067 0.001012 0.001327 0.001928 0.002092 0.001265 0.001291 0.001960 0.002077
Nuclear Periphery Foci 0.004241 0.002361 0.007685 0.005037 0.001100 0.000378 0.000163 0.010817 0.001561 0.000763 0.000969 0.007302
Nucleolus 0.002206 0.003613 0.001198 0.006664 0.002708 0.002788 0.001362 0.011411 0.002634 0.002948 0.001344 0.008524
Nucleus 0.216460* 0.368039* 0.615646* 0.485437* 0.472354* 0.475565* 0.580733* 0.439106* 0.434800* 0.454711* 0.584472* 0.467277*
Peroxisomes 0.011905 0.009505 0.003561 0.006043 0.002582 0.004577 0.000523 0.010717 0.003951 0.005533 0.000849 0.007875
Vacuole 0.048952 0.030885 0.081363 0.045432 0.010704 0.015611 0.009592 0.060126 0.016317 0.018573 0.017279 0.051191
Vacuole Periphery 0.001585 0.001564 0.000200 0.000592 0.000420 0.000831 0.000125 0.001030 0.000591 0.000973 0.000133 0.000764

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.57 -5.92 -1.01 -0.29 5.73 -2.38 -1.29 -6.92 -5.42 -6.44 -2.79 -4.91 -9.60 -6.21 -4.24
Bud Neck 0.55 -1.68 -2.58 -4.02 -0.32 -7.21 -11.97 -8.69 -3.11 2.14 -6.45 -11.88 -10.17 -3.28 3.93
Bud Site 1.59 -2.54 -0.29 -4.04 2.91 -6.15 -5.64 -9.27 -2.55 -4.35 -5.01 -5.51 -9.52 -2.83 -2.93
Cell Periphery 2.96 1.90 3.44 2.23 5.80 0.31 2.67 -6.69 -6.52 -7.56 2.36 3.36 -2.12 -6.25 -7.88
Cytoplasm 1.12 13.69 6.80 6.26 -11.22 1.92 9.71 15.16 13.48 7.48 2.24 12.93 16.74 14.64 3.92
Cytoplasmic Foci 5.59 15.28 13.31 9.18 -5.57 4.91 17.28 -3.24 -7.11 -15.68 5.74 22.08 5.25 -0.53 -18.81
Eisosomes 4.36 3.20 5.07 2.83 5.32 4.65 13.06 -10.98 -14.03 -18.63 5.30 8.83 -2.04 -12.14 -19.10
Endoplasmic Reticulum 1.98 -6.19 1.05 -2.41 7.58 0.05 -2.67 -12.28 -12.21 -11.69 1.02 -5.06 -13.11 -15.95 -6.33
Endosome 2.32 2.09 -0.84 -4.44 -3.89 0.14 4.72 -12.13 -12.20 -13.34 1.08 5.49 -13.55 -14.46 -16.98
Golgi -0.88 3.49 2.79 2.63 -1.12 0.49 3.21 -0.10 -0.81 -7.19 -0.18 4.07 1.02 1.33 -6.00
Lipid Particles 3.50 5.35 5.57 4.51 0.81 2.23 7.43 -3.36 -5.22 -9.66 3.46 8.95 2.34 -1.89 -12.33
Mitochondria 0.17 3.42 3.01 3.20 -1.99 -4.26 -1.25 -0.05 4.30 1.40 -4.18 0.60 1.96 5.11 2.31
Mitotic Spindle -0.05 -3.21 -5.39 -5.22 0.44 -1.07 -2.34 -2.81 -1.81 -0.60 -0.96 -3.17 -4.82 -3.89 -1.31
None -0.56 0.97 -0.21 0.58 -2.59 0.54 0.79 -0.33 -0.89 -1.28 -0.11 1.17 -1.28 -1.14 -3.35
Nuclear Periphery 1.66 -0.72 -0.46 -7.58 0.80 -1.82 -9.80 -7.91 -6.99 0.53 -0.19 -7.93 -8.01 -10.70 0.05
Nuclear Periphery Foci 1.34 -4.01 -3.75 -5.82 2.31 3.23 1.09 -8.66 -9.12 -8.84 2.72 -2.22 -11.22 -12.24 -9.72
Nucleolus -2.08 -1.43 -9.10 -7.24 -4.70 0.09 -2.87 -8.32 -8.36 -6.83 -0.33 -3.26 -11.26 -11.13 -8.43
Nucleus -8.54 -14.45 -15.73 -4.46 5.39 -0.51 -10.55 6.90 7.29 15.38 -2.50 -15.38 1.47 4.00 16.99
Peroxisomes 0.65 2.90 2.23 1.49 -1.52 -2.51 3.83 -6.18 -4.47 -7.49 -2.02 5.10 -5.56 -3.02 -9.77
Vacuole 3.90 -6.45 -3.35 -10.92 4.99 -4.64 -5.30 -18.05 -16.51 -16.06 -1.21 -6.36 -22.97 -22.55 -16.77
Vacuole Periphery -0.03 3.06 2.50 2.03 -2.12 -1.58 2.63 -1.44 0.02 -2.72 -1.60 3.90 -0.18 1.47 -4.06
Standard name
Human Ortholog
Description Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (24%), nucleus (10%), mixed (53%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Atg29

Atg29


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg29-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available