Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0.15 0.08 0.06 0.14 0.09 0.06 0 0 0 0 0 0 0.5 0.62 0.6 0.28 0.2 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.05 0.08 0.05 0.07 0 0 0 0 0 0
Endosome 0.07 0 0.17 0 0.11 0 0 0.05 0 0 0 0 0.11 0.14 0.12 0.14 0.18 0.19 0.12 0.09 0.07 0.05 0.07 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.75 0.91 0.71 0.78 0.83 0.9 0.94 0.88 0.93 0.97 0.96 0.97 0.16 0 0.06 0.2 0.1 0.14 0.71 0.68 0.71 0.74 0.68 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.28 0.14 0.09 0 0.05 0 0 0 0 0 0.05 0 0.07 0.06 0.2 0.16 0 0 0.08 0 0 0
SpindlePole 0.06 0 0.09 0.06 0.05 0 0.06 0.08 0 0 0 0 0.05 0.06 0 0.14 0.13 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.07 0.07 0 0.06 0.07 0 0.06 0.08 0.06 0.07 0.05 0.13 0.1 0.08 0.2 0.2 0.13 0 0.09 0 0.07 0.08 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 9 2 3 3 1 2 1 3 16 1 5 15
Bud 5 3 16 14 10 24 41 29 18 20 6 20 1 3 0 4 9 11 0 0 2 1 1 2
Bud Neck 1 0 1 0 0 0 0 0 0 1 0 0 2 2 1 0 0 2 1 0 1 2 1 7
Bud Site 2 0 0 0 0 8 20 9 7 7 0 3 0 0 0 0 1 1 – – – – – –
Cell Periphery 2 0 20 9 4 4 5 2 3 6 1 1 5 5 5 23 21 16 0 0 3 0 0 1
Cytoplasm 24 9 26 32 25 30 19 25 11 15 15 11 117 130 134 80 51 57 1 1 2 12 10 6
Endoplasmic Reticulum 0 7 2 1 2 1 0 2 2 9 8 21 12 19 12 24 12 16 3 0 2 3 1 2
Endosome 11 0 69 7 31 4 3 27 3 1 0 0 25 29 28 40 45 42 25 9 33 12 18 22
Golgi 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 3 2 4 2 0 14 1 6 6
Mitochondria 117 97 290 185 241 435 602 523 440 629 433 553 37 3 14 58 26 30 157 77 322 205 188 230
Nucleus 3 0 1 3 5 13 11 16 9 8 3 6 2 1 3 5 5 1 1 0 0 1 1 2
Nuclear Periphery 2 0 2 0 0 4 1 4 10 10 18 21 0 0 0 0 0 0 2 0 2 0 0 3
Nucleolus 4 0 0 0 1 5 12 6 3 7 5 2 0 0 0 0 1 1 0 0 1 2 1 2
Peroxisomes 4 0 114 33 27 17 29 18 8 1 1 1 12 0 16 18 50 34 6 3 35 3 3 2
SpindlePole 9 1 38 14 14 15 40 48 18 17 5 9 11 13 9 41 33 36 3 1 5 4 6 5
Vac/Vac Membrane 15 8 28 3 18 33 22 33 40 37 31 27 31 21 18 57 49 29 8 10 1 20 22 20
Unique Cell Count 155 107 410 237 292 482 640 597 471 650 452 572 235 210 224 287 250 219 222 113 452 279 278 338
Labelled Cell Count 199 126 608 301 378 593 805 742 573 768 526 675 264 228 243 356 306 282 222 113 452 279 278 338


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.2 4.6 4.3 4.3 3.9 3.8 4.1 3.7 3.6 3.5 3.5 5.7 5.7 5.8 6.5 6.1 5.8 5.7 6.1 6.4
Std Deviation (1e-4) 0.4 0.5 1.5 1.6 1.6 1.6 1.4 1.8 1.0 1.0 0.9 0.8 0.9 0.9 1.0 1.7 2.0 1.6 1.3 1.7 1.8
Intensity Change (Log2) – – – -0.09 -0.07 -0.23 -0.28 -0.17 -0.28 -0.34 -0.38 -0.38 0.33 0.32 0.34 0.52 0.43 0.34 0.34 0.43 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.2 5.0 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7661 1.6056 1.3731 1.6123 0.8173 1.5129 0.8168 0.1129 0.0935 -0.0027 0.9228 0.4855 -0.1433 -0.1312 -0.1453 -0.1715 -0.4283 -0.4041
Actin 0.0051 0.031 0.0182 0.0066 0.0211 0.0124 0.0783 0.023 0.0169 0.0349 0.0624 0.0258 0.0261 0.0103 0.0298 0.0043 0.0361 0.0274
Bud 0.0006 0.0028 0.0024 0.0115 0.0105 0.0012 0.0072 0.0045 0.0035 0.0034 0.0012 0.0013 0.0015 0.0047 0.0092 0.0082 0.004 0.0019
Bud Neck 0.0024 0.0021 0.0059 0.0036 0.0016 0.0116 0.0035 0.0022 0.004 0.0066 0.003 0.0066 0.0032 0.0015 0.0039 0.0022 0.0022 0.0079
Bud Periphery 0.0016 0.005 0.0074 0.049 0.0254 0.0054 0.0058 0.008 0.0064 0.0097 0.0016 0.0032 0.0032 0.0088 0.0115 0.0113 0.0146 0.0057
Bud Site 0.0091 0.0444 0.0207 0.0223 0.0115 0.0019 0.0332 0.0363 0.0338 0.0116 0.0413 0.0018 0.0086 0.0295 0.0295 0.0082 0.0037 0.0021
Cell Periphery 0.005 0.0021 0.0075 0.0037 0.0024 0.0034 0.0016 0.0034 0.005 0.0038 0.0009 0.0009 0.0016 0.0015 0.0019 0.0011 0.0008 0.0011
Cytoplasm 0.0371 0.0555 0.0555 0.1103 0.0354 0.0572 0.0425 0.1272 0.128 0.0979 0.067 0.0887 0.0408 0.0753 0.0641 0.0594 0.0854 0.0466
Cytoplasmic Foci 0.0914 0.1289 0.1002 0.1415 0.083 0.1235 0.1232 0.1285 0.1474 0.0966 0.0794 0.1552 0.156 0.1785 0.1198 0.1169 0.1657 0.1281
Eisosomes 0.0022 0.0016 0.0028 0.0005 0.0011 0.0007 0.0021 0.0017 0.0009 0.0006 0.0003 0.0003 0.0009 0.0005 0.0013 0.0004 0.0009 0.0005
Endoplasmic Reticulum 0.0025 0.0093 0.0068 0.0029 0.0038 0.0063 0.0082 0.0065 0.0064 0.0042 0.0219 0.0134 0.0062 0.0066 0.0035 0.0059 0.0018 0.0028
Endosome 0.1287 0.1081 0.1472 0.1541 0.1212 0.101 0.167 0.1465 0.1795 0.1026 0.2619 0.1592 0.2255 0.1684 0.1252 0.1701 0.1915 0.134
Golgi 0.0611 0.0799 0.0519 0.0765 0.043 0.0494 0.069 0.0769 0.0633 0.0519 0.1948 0.0788 0.0846 0.0966 0.0605 0.0444 0.1022 0.077
Lipid Particles 0.0478 0.0212 0.0191 0.0146 0.0193 0.0314 0.0629 0.0182 0.022 0.0544 0.0315 0.0323 0.0317 0.0113 0.0152 0.0302 0.0228 0.0157
Mitochondria 0.2548 0.344 0.3617 0.3088 0.3575 0.373 0.1837 0.1996 0.2264 0.2614 0.1888 0.2929 0.2666 0.2632 0.3246 0.35 0.2636 0.3845
None 0.2677 0.1221 0.1097 0.0536 0.1503 0.1645 0.1068 0.1562 0.0962 0.1258 0.0146 0.0775 0.0588 0.0832 0.1268 0.0956 0.0299 0.0709
Nuclear Periphery 0.0006 0.0012 0.0028 0.0006 0.0009 0.0006 0.0094 0.0018 0.0028 0.0021 0.0015 0.0041 0.009 0.002 0.0012 0.003 0.0005 0.0023
Nucleolus 0.0014 0.0005 0.002 0.0016 0.0009 0.0007 0.0026 0.0011 0.0011 0.0046 0.0002 0.0005 0.0008 0.0008 0.0042 0.002 0.0002 0.0006
Nucleus 0.0009 0.0008 0.0019 0.0019 0.001 0.0007 0.0031 0.0019 0.0019 0.0026 0.0007 0.001 0.0033 0.0012 0.0021 0.0052 0.0006 0.0016
Peroxisomes 0.0557 0.0305 0.0503 0.0198 0.0969 0.0405 0.0471 0.0374 0.0281 0.0791 0.0126 0.0423 0.054 0.0437 0.0535 0.0551 0.0632 0.0768
Punctate Nuclear 0.0005 0.0009 0.0009 0.0023 0.0017 0.0009 0.0192 0.001 0.0026 0.0043 0.0005 0.0011 0.0019 0.0009 0.0017 0.0103 0.001 0.0037
Vacuole 0.0188 0.0056 0.0196 0.0108 0.0074 0.0093 0.0194 0.0139 0.0189 0.035 0.0087 0.0077 0.0107 0.0079 0.007 0.0106 0.0058 0.0054
Vacuole Periphery 0.0048 0.0027 0.0056 0.0035 0.0042 0.0043 0.0041 0.004 0.0049 0.0069 0.005 0.005 0.0049 0.0037 0.0035 0.0056 0.0035 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4003 4.5772 2.8304 8.3951 9.4046 5.9703 9.4487 7.8089 8.9676 10.4349
Translational Efficiency 0.9728 1.0877 1.6569 0.5812 0.5877 1.0852 0.617 0.6738 0.6755 0.646

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2183 856 238 1634 2289 1953 150 222 4472 2809 388 1856

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 690.23 773.56 884.82 860.18 684.78 749.54 909.64 946.28 687.44 756.86 894.42 870.48
Standard Deviation 82.60 103.60 91.86 117.11 85.12 89.44 103.61 110.03 83.94 94.63 97.32 119.60
Intensity Change Log 2 — 0.164436 0.358307 0.317561 — 0.130365 0.409655 0.466627 — 0.147569 0.384108 0.393723

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000860 0.002758 0.005161 0.014929 0.001051 0.000787 0.003985 0.010733 0.000958 0.001388 0.004706 0.014427
Bud Neck 0.020760 0.023177 0.019139 0.030025 0.022952 0.018446 0.013859 0.037326 0.021882 0.019887 0.017097 0.030898
Bud Site 0.015030 0.034487 0.029609 0.127728* 0.015863 0.020761 0.033028 0.114265* 0.015456 0.024944 0.030931 0.126118*
Cell Periphery 0.000289 0.000325 0.000422 0.000252 0.000435 0.000212 0.000386 0.000201 0.000364 0.000246 0.000408 0.000246
Cytoplasm 0.031169 0.027401 0.174424* 0.144912* 0.043183 0.026167 0.160594* 0.158552* 0.037318 0.026543 0.169077* 0.146543*
Cytoplasmic Foci 0.142625 0.152861 0.136783 0.146763 0.211068* 0.145741 0.204347* 0.171359 0.177658 0.147911 0.162903 0.149705
Eisosomes 0.000233 0.000180 0.000242 0.000196 0.000236 0.000131 0.000268 0.000191 0.000235 0.000146 0.000252 0.000195
Endoplasmic Reticulum 0.001764 0.000587 0.016579 0.006271 0.001996 0.000592 0.007334 0.002901 0.001883 0.000590 0.013005 0.005868
Endosome 0.070747 0.106610 0.031293 0.037041 0.066851 0.094000 0.036573 0.030033 0.068753 0.097843 0.033334 0.036203
Golgi 0.115549* 0.143280* 0.004648 0.026001 0.101325 0.126421* 0.003771 0.008547 0.108268 0.131559* 0.004309 0.023913
Lipid Particles 0.010379 0.004309 0.019289 0.003248 0.015897 0.006039 0.015568 0.003521 0.013203 0.005512 0.017851 0.003281
Mitochondria 0.511378* 0.315125* 0.005115 0.057932 0.419767* 0.422703* 0.007464 0.005428 0.464487* 0.389920* 0.006023 0.051652
Mitotic Spindle 0.006193 0.033260 0.036260 0.088767 0.006904 0.022814 0.029443 0.049503 0.006557 0.025997 0.033624 0.084070
None 0.000728 0.001048 0.001924 0.000535 0.000700 0.000608 0.001725 0.000721 0.000714 0.000742 0.001847 0.000557
Nuclear Periphery 0.000183 0.000139 0.003331 0.000874 0.000432 0.000180 0.001630 0.000497 0.000310 0.000168 0.002673 0.000829
Nuclear Periphery Foci 0.000200 0.000192 0.007702 0.000896 0.000336 0.000266 0.003967 0.001282 0.000270 0.000244 0.006258 0.000942
Nucleolus 0.000780 0.001568 0.008190 0.001162 0.000697 0.001078 0.004543 0.000862 0.000737 0.001227 0.006780 0.001126
Nucleus 0.004672 0.005263 0.314663* 0.036133 0.006391 0.004479 0.205129* 0.047620 0.005552 0.004718 0.272317* 0.037507
Peroxisomes 0.030226 0.105654* 0.038731 0.096912 0.035181 0.053873 0.098802 0.090583 0.032763 0.069652 0.061954 0.096155
Vacuole 0.020514 0.027314 0.143779 0.168626 0.033533 0.038356 0.164975 0.264800* 0.027178 0.034991 0.151974 0.180129
Vacuole Periphery 0.015720 0.014462 0.002716 0.010797 0.015201 0.016349 0.002610 0.001074 0.015455 0.015774 0.002675 0.009634

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.75 -5.63 -11.89 -9.43 -5.75 1.16 -5.21 -4.11 -4.25 -2.62 -2.41 -7.13 -12.44 -11.95 -7.15
Bud Neck -0.87 0.27 -4.82 -2.70 -3.22 2.10 3.55 -1.63 -2.58 -3.33 1.19 1.80 -5.14 -5.98 -5.39
Bud Site -6.38 -3.84 -23.00 -15.50 -15.06 -2.83 -2.05 -7.46 -6.95 -4.96 -6.26 -4.00 -24.39 -21.01 -15.21
Cell Periphery -0.77 -3.56 2.65 2.38 5.76 1.14 0.25 1.29 0.82 3.26 1.15 -0.52 1.45 1.15 6.07
Cytoplasm 1.04 -10.96 -23.70 -22.65 1.06 6.28 -7.78 -7.97 -9.24 -0.05 5.22 -13.28 -24.46 -26.54 0.77
Cytoplasmic Foci -1.66 1.21 2.55 3.62 0.05 12.16 2.06 6.92 1.23 2.82 7.44 3.24 10.61 3.92 2.26
Eisosomes 2.68 -1.32 3.25 -0.01 4.57 5.71 -1.70 2.18 -2.70 3.60 6.97 -2.06 4.04 -3.76 6.05
Endoplasmic Reticulum 3.41 -8.99 -5.97 -8.20 6.07 4.88 -7.00 -4.27 -9.20 4.96 5.79 -10.63 -6.43 -8.86 6.35
Endosome -6.47 9.50 13.43 14.46 -0.44 -7.24 7.29 12.50 17.18 1.90 -9.43 12.01 17.76 22.72 0.07
Golgi -3.82 29.53 24.34 19.15 -7.87 -5.02 30.91 27.27 29.24 -1.19 -5.70 41.85 32.31 31.62 -8.91
Lipid Particles 5.22 -4.00 8.11 0.96 7.51 7.76 0.31 10.15 2.68 5.95 8.96 -2.83 13.48 3.67 9.55
Mitochondria 15.19 71.02* 60.24* 23.78 -13.63 -0.22 57.61* 59.05* 54.61* 0.81 9.45 90.69* 74.81* 51.24* -12.86
Mitotic Spindle -7.33 -3.50 -17.20 -9.38 -6.45 -7.79 -2.90 -4.69 -2.92 -2.10 -10.71 -4.47 -17.87 -12.52 -8.00
None -0.84 -2.80 1.02 1.44 3.27 0.42 -2.44 0.41 -0.03 2.64 -0.09 -3.67 1.03 0.93 4.01
Nuclear Periphery 0.91 -7.39 -9.98 -9.92 5.15 1.96 -5.55 -2.53 -6.27 4.24 2.03 -8.55 -7.79 -10.74 5.78
Nuclear Periphery Foci 0.24 -7.35 -10.80 -11.43 6.54 0.54 -6.49 -5.87 -6.04 4.10 0.33 -9.08 -10.78 -9.70 7.82
Nucleolus -1.45 -3.50 -2.90 -0.01 3.16 -1.86 -3.56 -0.99 1.15 3.41 -2.22 -4.33 -3.47 -0.78 3.81
Nucleus -0.57 -16.82 -16.40 -15.41 14.24 2.07 -10.17 -6.62 -6.85 7.25 1.09 -19.33 -17.59 -17.66 15.88
Peroxisomes -11.81 -1.36 -12.64 3.34 -6.92 -5.92 -3.32 -3.51 -1.39 0.82 -12.61 -3.39 -12.89 -2.29 -3.67
Vacuole -2.81 -13.35 -33.45 -30.51 -4.78 -1.87 -10.09 -16.60 -16.33 -5.67 -4.27 -16.22 -35.75 -33.71 -5.03
Vacuole Periphery 0.82 16.27 5.90 3.41 -5.79 -1.07 15.82 19.95 19.48 2.66 -0.35 22.49 7.77 7.27 -5.87
Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrx4

Mrx4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available