Standard name
Human Ortholog
Description Heme A:farnesyltransferase; catalyzes first step in conversion of protoheme to heme A prosthetic group required for cytochrome c oxidase activity; human ortholog COX10 can complement yeast cox10 null mutant; human ortholog COX10 is associated with mitochondrial disorders
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0.06 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.88 0.66 0.76 0.82 0.62 0.5 0.44 0.38 – 0.31 0.3 0.19 0.97 0.97 0.98 – – 0.55 0.44 0.46 0.32 0.46 0.43 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.08 0.42 0.18 0.23 0.29 0.55 0.61 0.6 – 0.75 0.79 0.85 0 0 0 – – 0.16 0.36 0.35 0.45 0.37 0.37 0.25
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0.05 0.06 0.09 – 0.06 0.05 0.08 0 0 0 – – 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0 0 – 0 0 0 0 0 0 – – 0.07 0 0.07 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 2 1 9 4 6 7
Bud 0 1 0 5 9 4 8 16 – 12 18 27 0 0 0 – – 3 0 0 0 1 3 10
Bud Neck 1 0 0 0 0 0 3 3 – 2 4 2 0 1 0 – – 2 0 0 4 0 5 12
Bud Site 0 0 0 2 2 0 7 5 – 9 5 9 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 3 0 1 3 1 0 – 2 2 1 5 2 2 – – 2 1 0 3 0 2 2
Cytoplasm 107 83 168 211 171 169 205 211 – 151 98 83 172 170 215 – – 41 63 62 88 86 132 190
Endoplasmic Reticulum 2 1 3 0 0 0 0 0 – 0 0 0 4 2 0 – – 7 1 0 4 0 3 4
Endosome 4 0 3 5 25 9 11 12 – 1 0 0 1 0 1 – – 2 5 5 14 5 9 4
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 1 0 0 0 0 1
Mitochondria 10 52 40 60 80 188 285 332 – 364 263 382 1 0 0 – – 12 51 48 123 70 113 87
Nucleus 0 0 0 0 2 4 4 13 – 7 5 2 0 1 1 – – 1 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 1 0 1 0 – 2 1 2 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 – 2 4 0 0 0 0 – – 1 0 0 0 0 0 0
Peroxisomes 0 0 4 4 4 4 9 5 – 3 0 7 1 0 0 – – 3 0 0 2 0 2 0
SpindlePole 0 0 10 5 9 17 26 48 – 31 16 34 1 0 0 – – 8 4 1 6 2 9 9
Vac/Vac Membrane 1 2 8 4 19 12 15 10 – 4 4 9 0 2 5 – – 5 4 9 6 9 10 10
Unique Cell Count 122 125 222 258 277 341 464 550 485 332 447 178 175 219 75 144 137 277 190 306 351
Labelled Cell Count 127 140 239 296 323 410 576 656 590 420 558 185 178 224 87 144 137 277 190 306 351


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.5 3.4 3.7 3.7 3.2 3.0 3.3 – 2.6 2.7 2.9 4.8 5.1 5.2 3.8 4.1 5.0 4.9 5.1 5.4
Std Deviation (1e-4) 0.8 0.7 0.9 1.7 1.8 1.7 1.4 1.4 – 0.9 1.1 0.8 0.8 1.0 0.9 1.1 1.3 2.1 1.1 1.6 1.9
Intensity Change (Log2) – – – 0.12 0.14 -0.07 -0.18 -0.03 – -0.4 -0.31 -0.23 0.49 0.6 0.61 0.15 0.29 0.57 0.54 0.59 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3865 0.3548 1.0066 0.4029 -0.012 0.5452 0.9738 1.2954 1.2874 1.1678 1.1362 1.1092 0.7209 2.1742 1.3599 1.4562 0.7895 1.2426
Actin 0.0369 0.0093 0.0339 0.001 0.0093 0.0197 0.0118 0.0173 0.0223 0.0354 0.0328 0.0092 0.0063 0.0112 0.0204 0.0084 0.0051 0.0068
Bud 0.0014 0.0019 0.0006 0.0008 0.0005 0.001 0.0018 0.0083 0.0012 0.0011 0.0013 0.0009 0.0013 0.001 0.0021 0.0049 0.0016 0.0017
Bud Neck 0.0043 0.0012 0.0056 0.001 0.0005 0.0009 0.0022 0.0018 0.003 0.001 0.0015 0.0094 0.0008 0.0016 0.0135 0.001 0.0007 0.0079
Bud Periphery 0.0032 0.0037 0.0015 0.0023 0.0024 0.004 0.0039 0.0186 0.0031 0.0084 0.0045 0.0028 0.0035 0.0025 0.0047 0.0255 0.0043 0.0092
Bud Site 0.0071 0.0173 0.0032 0.0014 0.0008 0.0007 0.016 0.0115 0.005 0.0043 0.0029 0.001 0.0038 0.0193 0.0072 0.0018 0.0026 0.001
Cell Periphery 0.0062 0.0016 0.0011 0.0054 0.0007 0.0007 0.0014 0.0013 0.0017 0.0012 0.0024 0.0013 0.0015 0.0027 0.0019 0.0017 0.0008 0.0016
Cytoplasm 0.0485 0.101 0.0857 0.1093 0.0999 0.0781 0.0704 0.1276 0.1022 0.0584 0.0779 0.1311 0.128 0.17 0.1435 0.1436 0.1079 0.1836
Cytoplasmic Foci 0.0747 0.0367 0.0367 0.0759 0.0533 0.0547 0.1107 0.1068 0.0929 0.1015 0.099 0.0962 0.1065 0.115 0.0679 0.0631 0.1465 0.0816
Eisosomes 0.0014 0.0007 0.0014 0.0005 0.0008 0.0004 0.0008 0.0004 0.001 0.0007 0.0006 0.0004 0.0008 0.0009 0.0012 0.0006 0.0003 0.0004
Endoplasmic Reticulum 0.0058 0.0026 0.0143 0.0059 0.0125 0.0061 0.0044 0.0023 0.0051 0.003 0.0023 0.0025 0.0121 0.0055 0.0067 0.0103 0.0022 0.0054
Endosome 0.0491 0.0191 0.0313 0.0917 0.1046 0.0272 0.071 0.0599 0.0602 0.0671 0.059 0.0567 0.1009 0.0488 0.0814 0.0855 0.1406 0.0714
Golgi 0.0181 0.0076 0.009 0.0307 0.0363 0.013 0.0242 0.0221 0.0255 0.0111 0.0472 0.0141 0.0456 0.0276 0.0243 0.0243 0.0405 0.0304
Lipid Particles 0.0113 0.0111 0.0133 0.0281 0.0307 0.0098 0.0287 0.011 0.0148 0.0205 0.019 0.0102 0.0298 0.0122 0.0084 0.0046 0.0176 0.0117
Mitochondria 0.06 0.0284 0.0417 0.0444 0.155 0.0722 0.1001 0.0782 0.1162 0.185 0.1904 0.0762 0.1161 0.0969 0.1356 0.1918 0.1232 0.1675
None 0.5511 0.7425 0.6889 0.5055 0.4696 0.6776 0.4927 0.4893 0.5013 0.4383 0.408 0.5578 0.4021 0.4448 0.4526 0.405 0.3448 0.3986
Nuclear Periphery 0.0269 0.0006 0.0076 0.0059 0.0013 0.0011 0.003 0.0009 0.0014 0.0017 0.0096 0.0005 0.0024 0.001 0.0023 0.0014 0.0005 0.0006
Nucleolus 0.0026 0.001 0.0015 0.0027 0.0003 0.0009 0.0013 0.0007 0.0006 0.0008 0.0006 0.0003 0.0007 0.0016 0.0006 0.0005 0.0003 0.0003
Nucleus 0.038 0.0014 0.0061 0.0523 0.0009 0.0014 0.003 0.0012 0.001 0.0012 0.0012 0.0006 0.0011 0.0012 0.001 0.0013 0.0007 0.0007
Peroxisomes 0.0101 0.0057 0.0046 0.0103 0.0113 0.0179 0.0352 0.0307 0.0303 0.0451 0.0241 0.023 0.0237 0.027 0.013 0.0067 0.047 0.012
Punctate Nuclear 0.0218 0.001 0.0033 0.0021 0.0008 0.0058 0.0094 0.0016 0.0022 0.004 0.0099 0.0014 0.0022 0.0012 0.0007 0.0005 0.0011 0.0006
Vacuole 0.0165 0.0047 0.0071 0.0193 0.0052 0.005 0.006 0.007 0.0067 0.007 0.0035 0.0034 0.008 0.0066 0.0082 0.0141 0.0078 0.0052
Vacuole Periphery 0.0051 0.0007 0.0016 0.0036 0.0031 0.0017 0.0021 0.0013 0.0023 0.0031 0.0024 0.0012 0.0027 0.0013 0.0031 0.0033 0.0039 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5046 7.8115 7.9043 12.6323 8.7546 7.1455 12.7448 15.9922 12.7747 16.9779
Translational Efficiency 0.4775 0.4787 0.6254 0.5793 0.7051 1.2979 0.6334 0.5871 0.7721 0.4638

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
144 985 301 1622 177 1446 1718 1505 321 2431 2019 3127

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 850.68 689.93 854.96 825.30 612.50 713.62 750.47 805.72 719.35 704.02 766.05 815.88
Standard Deviation 114.99 103.59 84.36 116.79 70.13 84.83 89.46 95.09 150.59 93.61 96.21 107.34
Intensity Change Log 2 — -0.302167 0.007240 -0.043698 — 0.220446 0.293085 0.395569 — -0.060027 0.133853 0.156667

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003322 0.000455 0.000691 0.003319 0.000224 0.000557 0.000290 0.002955 0.001614 0.000516 0.000350 0.003143
Bud Neck 0.018885 0.040963 0.004490 0.017093 0.058996 0.055024 0.006362 0.015784 0.041002 0.049327 0.006083 0.016463
Bud Site 0.031146 0.014448 0.005451 0.050714 0.007643 0.012614 0.008284 0.042920 0.018186 0.013357 0.007861 0.046963
Cell Periphery 0.004373 0.000173 0.000223 0.000252 0.000313 0.000368 0.000114 0.000247 0.002134 0.000289 0.000130 0.000250
Cytoplasm 0.206409* 0.353270* 0.259522* 0.379006* 0.247464* 0.323026* 0.416909* 0.381960* 0.229047* 0.335280* 0.393446* 0.380428*
Cytoplasmic Foci 0.108084 0.262901* 0.026905 0.057024 0.387573* 0.311825* 0.020290 0.077879 0.262195* 0.292002* 0.021276 0.067061
Eisosomes 0.001081 0.000169 0.000081 0.000121 0.000574 0.000279 0.000036 0.000129 0.000801 0.000235 0.000043 0.000125
Endoplasmic Reticulum 0.011535 0.000935 0.008289 0.004186 0.000491 0.000588 0.002397 0.001796 0.005445 0.000728 0.003275 0.003036
Endosome 0.031336 0.035386 0.005546 0.012148 0.031749 0.034754 0.002501 0.008086 0.031564 0.035010 0.002955 0.010193
Golgi 0.013811 0.006941 0.000159 0.008107 0.010987 0.012615 0.000328 0.004975 0.012254 0.010316 0.000303 0.006600
Lipid Particles 0.004443 0.014193 0.003900 0.002840 0.033394 0.012865 0.001522 0.002858 0.020407 0.013403 0.001876 0.002848
Mitochondria 0.007182 0.025133 0.001601 0.007906 0.046805 0.034024 0.000850 0.006678 0.029030 0.030422 0.000962 0.007315
Mitotic Spindle 0.016987 0.003805 0.000960 0.036642 0.000153 0.003362 0.004621 0.016463 0.007704 0.003541 0.004075 0.026930
None 0.000664 0.004033 0.003344 0.002139 0.000786 0.001495 0.004526 0.002198 0.000731 0.002523 0.004350 0.002167
Nuclear Periphery 0.000965 0.000692 0.002189 0.001525 0.000438 0.000281 0.001404 0.001041 0.000674 0.000448 0.001521 0.001292
Nuclear Periphery Foci 0.001223 0.000564 0.002523 0.001303 0.000215 0.000159 0.001001 0.000571 0.000667 0.000323 0.001228 0.000951
Nucleolus 0.001068 0.003418 0.001079 0.000883 0.002310 0.001704 0.000479 0.000588 0.001753 0.002398 0.000568 0.000741
Nucleus 0.211085* 0.137181* 0.598917* 0.242083* 0.088935 0.097023 0.484780* 0.295683* 0.143731* 0.113294 0.501796* 0.267880*
Peroxisomes 0.024024 0.025831 0.000952 0.021000 0.038481 0.033507 0.001567 0.034909 0.031995 0.030397 0.001475 0.027694
Vacuole 0.293766* 0.065796 0.072688 0.148795 0.039758 0.061280 0.041526 0.101466 0.153706 0.063110 0.046172 0.126016
Vacuole Periphery 0.008611 0.003712 0.000489 0.002917 0.002710 0.002650 0.000215 0.000816 0.005358 0.003080 0.000256 0.001906

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.10 1.76 0.60 -4.61 -3.50 -1.97 -1.67 -5.13 -3.93 -4.90 1.66 1.94 -1.38 -5.87 -6.78
Bud Neck -5.03 3.15 0.82 7.80 -4.18 0.41 5.61 4.88 12.44 -4.97 -1.51 6.13 4.79 14.64 -6.98
Bud Site 2.27 3.79 -0.86 -5.97 -8.66 -1.59 0.13 -6.31 -6.79 -8.61 0.98 2.97 -4.58 -9.10 -11.74
Cell Periphery 1.26 1.23 1.25 -1.16 1.67 -0.45 4.66 2.38 1.51 -3.48 1.22 1.36 1.31 1.42 -4.27
Cytoplasm -7.65 -1.89 -7.25 1.30 -6.54 -4.29 -6.81 -6.06 -3.13 1.32 -8.23 -9.06 -9.22 -1.44 0.36
Cytoplasmic Foci -14.27 6.77 5.15 29.99 -4.70 4.24 21.83 18.91 36.06 -13.48 -2.62 17.93 15.32 47.72 -15.16
Eisosomes 1.00 1.12 1.11 6.06 -0.67 4.63 8.60 7.35 8.11 -7.16 1.56 2.20 2.00 10.00 -9.39
Endoplasmic Reticulum 2.22 -0.05 1.43 -5.20 5.54 -0.56 -14.63 -11.86 -9.65 4.74 2.18 0.15 0.78 -7.78 3.07
Endosome -1.80 3.92 3.60 9.63 -1.64 -0.51 5.53 4.78 14.73 -6.49 -1.53 7.09 5.96 17.08 -7.92
Golgi 0.57 1.81 1.07 2.44 -4.60 -0.40 2.92 2.08 6.57 -3.57 0.08 3.26 2.15 6.85 -5.72
Lipid Particles -6.32 0.24 2.74 8.03 3.50 3.34 5.24 5.05 9.17 -4.93 1.97 5.33 5.12 12.22 -3.92
Mitochondria -4.97 1.44 0.31 6.87 -2.78 1.14 4.17 3.82 9.11 -4.10 -0.28 4.33 3.75 11.37 -5.44
Mitotic Spindle 1.63 1.95 -0.73 -7.22 -8.71 -4.35 -5.16 -6.92 -4.85 -3.69 1.15 0.85 -2.63 -8.78 -7.69
None -5.90 -5.40 -7.90 3.22 1.54 -2.42 -10.46 -4.83 -2.33 6.33 -6.23 -13.06 -8.22 1.28 7.18
Nuclear Periphery 2.54 -6.08 -6.15 -12.00 1.20 1.37 -11.16 -8.82 -19.41 3.45 3.02 -10.55 -10.52 -21.03 0.12
Nuclear Periphery Foci 2.70 -3.80 0.28 -5.32 5.08 0.81 -4.42 -6.02 -9.79 2.08 2.91 -3.17 -2.02 -10.03 2.20
Nucleolus -3.87 0.09 0.58 6.15 0.92 0.41 1.26 1.23 4.48 -0.61 -0.85 1.41 1.32 7.41 -0.85
Nucleus 3.15 -12.76 -2.47 -16.30 17.42 -0.87 -38.27 -20.45 -27.31 19.37 2.53 -27.43 -11.52 -31.71 28.24
Peroxisomes -1.28 3.35 1.23 5.03 -6.18 0.68 5.23 1.91 2.79 -8.31 -0.17 6.15 2.45 5.81 -10.21
Vacuole 11.06 9.26 4.46 -22.64 -12.32 -3.69 -3.75 -15.15 -15.76 -15.41 7.72 7.38 -1.18 -27.63 -24.99
Vacuole Periphery 0.54 1.22 0.99 3.37 -3.28 0.09 3.14 2.63 6.73 -4.16 0.57 1.88 1.48 6.03 -4.90
Standard name
Human Ortholog
Description Heme A:farnesyltransferase; catalyzes first step in conversion of protoheme to heme A prosthetic group required for cytochrome c oxidase activity; human ortholog COX10 can complement yeast cox10 null mutant; human ortholog COX10 is associated with mitochondrial disorders
Localization
Cell Percentages mitochondrion (17%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Cox10

Cox10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cox10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available