Standard name
Human Ortholog
Description Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0.06 0 0 0 0 0 0.05 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.1 0.17 0.08 0.07 0.09 0.09 0.06 0.06 0.09 0.12 0.14 0.3 – 0.11 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 – 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0.05 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0
Mitochondria 0.1 0.35 – 0.08 0.33 0.43 0.22 0.26 0.83 0.58 0.65 0.68 0 0 0 – 0.06 0 0 0 0 0 0 0
Nucleus 0.88 0.78 – 0.74 0.67 0.58 0.77 0.76 0.52 0.68 0.64 0.69 0.8 0.83 0.63 – 0.69 0.64 0.71 0.69 0.65 0.64 0.67 0.62
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0.06 0 0.09 0.1 0.1
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0.06 0 0 0.07 0 0 0 0 0 0 0 – 0.05 0 0.05 0 0 0 0 0.07
Vac/Vac Membrane 0.06 0 – 0.08 0 0 0 0 0 0 0 0 0 0 0 – 0.1 0.13 0.08 0.11 0.17 0.08 0.09 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0
Bud 0 1 – 0 0 1 1 0 2 4 0 1 0 0 0 – 0 0 5 3 0 2 4 1
Bud Neck 0 2 – 0 0 0 0 4 2 2 0 1 0 1 1 – 0 1 1 1 0 0 0 0
Bud Site 0 0 – 0 1 0 1 1 4 4 4 3 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 0
Cytoplasm 9 20 – 4 3 6 7 13 13 8 5 7 21 34 71 – 27 29 5 10 1 4 6 5
Endoplasmic Reticulum 0 3 – 0 1 0 1 0 0 0 0 0 0 0 0 – 12 15 0 0 0 0 0 1
Endosome 3 1 – 0 0 2 0 0 0 0 1 0 4 2 5 – 20 9 5 10 0 0 2 4
Golgi 0 1 – 0 0 1 0 0 0 0 0 0 0 0 0 – 2 3 2 5 2 1 0 0
Mitochondria 27 175 – 3 6 33 22 36 125 84 52 52 5 1 3 – 15 6 3 12 0 2 3 7
Nucleus 241 387 – 29 12 44 76 107 78 98 51 53 138 208 148 – 175 120 217 402 30 88 124 119
Nuclear Periphery 0 0 – 1 0 1 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0
Nucleolus 2 2 – 0 0 0 1 0 0 2 1 0 1 0 0 – 0 1 18 35 1 12 17 19
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 2 0 0 1 0 0 0 0
SpindlePole 6 17 – 0 1 1 3 10 0 5 2 0 5 4 2 – 12 7 15 24 0 6 5 13
Vac/Vac Membrane 17 10 – 3 0 3 1 4 3 2 1 1 6 6 10 – 25 24 24 67 7 11 17 13
Unique Cell Count 275 498 39 18 76 99 141 151 145 80 77 172 250 235 252 188 305 586 47 138 186 191
Labelled Cell Count 305 619 40 24 92 114 175 228 209 117 118 181 256 241 290 215 305 586 47 138 186 191


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.0 – 4.8 3.8 4.1 4.1 4.2 3.3 3.6 3.5 3.6 5.4 5.5 4.7 4.8 6.2 6.5 5.9 6.2 6.5
Std Deviation (1e-4) 0.7 0.6 – 1.1 0.4 0.8 1.1 0.6 0.5 0.8 0.5 0.5 0.8 1.2 0.9 1.2 1.5 1.4 1.4 1.4 1.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8064 2.4551 2.5212 2.2312 2.5894 2.6302 5.6163 5.6144 5.7083 5.9557 5.5708 5.4886 2.6957 2.6553 2.6749 2.7603 2.8604 2.6866
Actin 0.0482 0.0001 0.001 0.0682 0.0484 0.0008 0.0228 0.0002 0.0106 0.0007 0.0095 0.0023 0.0032 0.0001 0.0001 0.0001 0.0001 0
Bud 0.0007 0.0001 0.0001 0.0017 0.0006 0.0002 0.0007 0.0004 0.0003 0.0025 0.0002 0.0001 0.0001 0 0 0 0.0001 0
Bud Neck 0.002 0.0005 0.0003 0.001 0.0027 0.0042 0.005 0.0015 0.0006 0.0014 0.0013 0.0016 0.0005 0.0003 0.0005 0.0002 0.0013 0.002
Bud Periphery 0.0022 0 0.0003 0.0044 0.0008 0.0004 0.0011 0.0003 0.0007 0.005 0.0002 0.0001 0.0001 0 0 0 0 0
Bud Site 0.0019 0.0004 0.001 0.0019 0.003 0.0011 0.0056 0.0029 0.0011 0.0204 0.0007 0.0001 0.0004 0.0002 0.0002 0 0.0001 0.0001
Cell Periphery 0.0005 0 0.0006 0.0003 0.0002 0.0001 0.0006 0.0001 0.0001 0.0005 0 0 0 0 0 0 0 0
Cytoplasm 0.0098 0.0037 0.0056 0.0011 0.028 0.0018 0.0158 0.0141 0.0031 0.0025 0.0091 0.0014 0.0096 0.0061 0.0036 0.0035 0.0039 0.0036
Cytoplasmic Foci 0.0155 0.0001 0.0011 0.0033 0.002 0.0023 0.0041 0.0008 0.0096 0.0027 0.015 0.0013 0.0088 0.0002 0.0002 0.0002 0.0003 0.0001
Eisosomes 0.0005 0.0001 0.0007 0.0003 0.0012 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0109 0.0003 0.0007 0.0012 0.0038 0.0005 0.0052 0.0019 0.003 0.0007 0.0009 0.0013 0.0009 0.0002 0.0002 0.0001 0.0001 0.0001
Endosome 0.0286 0.0002 0.0013 0.0185 0.0085 0.0059 0.0124 0.0013 0.0134 0.0082 0.0219 0.0105 0.0051 0.0002 0.0005 0.0002 0.0003 0.0001
Golgi 0.0119 0 0.0009 0.0105 0.0027 0.0038 0.0074 0.0001 0.0056 0.0008 0.0083 0.0015 0.0009 0 0 0 0.0001 0
Lipid Particles 0.032 0 0.0006 0.0099 0.004 0.0007 0.0038 0.0002 0.006 0.0026 0.0182 0.0011 0.0025 0 0.0001 0 0.0002 0
Mitochondria 0.0107 0.0002 0.0053 0.0414 0.0072 0.0014 0.0053 0.0003 0.0034 0.0007 0.0031 0.0017 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001
None 0.0235 0.0166 0.0355 0.0019 0.0029 0.0021 0.0202 0.0276 0.0166 0.0018 0.0244 0.0007 0.0116 0.0109 0.0089 0.0137 0.0375 0.0056
Nuclear Periphery 0.0278 0.0092 0.0082 0.0082 0.0188 0.002 0.0372 0.0086 0.0133 0.0032 0.0184 0.0101 0.0089 0.0042 0.0026 0.0052 0.0034 0.0025
Nucleolus 0.0192 0.0183 0.0147 0.0056 0.0723 0.0488 0.0311 0.0263 0.0162 0.0133 0.0328 0.0359 0.0156 0.014 0.0135 0.0078 0.0255 0.0389
Nucleus 0.6947 0.9356 0.912 0.806 0.7647 0.9102 0.776 0.8779 0.8527 0.9137 0.7776 0.9054 0.8737 0.9268 0.9435 0.9583 0.8488 0.9336
Peroxisomes 0.0322 0 0.0012 0.005 0.0031 0.0002 0.0031 0.0001 0.0033 0.001 0.0256 0.0004 0.0041 0 0 0 0 0
Punctate Nuclear 0.0159 0.0141 0.0082 0.0028 0.0196 0.0122 0.0383 0.0336 0.038 0.0064 0.0313 0.0236 0.0528 0.0362 0.0257 0.0105 0.0777 0.0131
Vacuole 0.0065 0.0003 0.0006 0.0033 0.0038 0.0009 0.0027 0.0014 0.0011 0.0111 0.0007 0.0004 0.0005 0.0002 0.0002 0 0.0001 0.0001
Vacuole Periphery 0.0048 0.0001 0.0003 0.0035 0.0017 0.0002 0.0013 0.0002 0.0011 0.0008 0.0006 0.0004 0.0002 0 0.0001 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9805 25.7036 15.8943 30.03 14.7566 14.3686 16.7845 27.042 25.3701 29.7453
Translational Efficiency 1.8207 1.0606 2.178 1.1785 2.2587 1.9028 1.8289 1.5722 1.7789 1.4717

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2365 822 204 1698 1430 1717 2427 1264 3795 2539 2631 2962

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 763.00 723.15 973.33 905.31 623.29 875.75 889.52 851.37 710.36 826.35 896.02 882.29
Standard Deviation 97.76 124.94 100.30 124.12 91.59 108.25 119.98 123.04 117.05 134.45 120.67 126.50
Intensity Change Log 2 — -0.077388 0.351246 0.246729 — 0.490616 0.513123 0.449883 — 0.205851 0.426282 0.341622

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000165 0.001290 0.001575 0.000834 0.000188 0.000275 0.000635 0.001159 0.000174 0.000603 0.000708 0.000973
Bud Neck 0.003893 0.010509 0.007707 0.026386 0.002766 0.009366 0.032410 0.016248 0.003468 0.009736 0.030495 0.022059
Bud Site 0.001251 0.005150 0.001638 0.010391 0.000806 0.002566 0.004268 0.016233 0.001083 0.003403 0.004064 0.012884
Cell Periphery 0.000087 0.000292 0.000074 0.000156 0.000195 0.000130 0.000109 0.000186 0.000128 0.000183 0.000106 0.000169
Cytoplasm 0.025165 0.057121 0.001880 0.034945 0.055900 0.037268 0.013122 0.074344 0.036746 0.043696 0.012251 0.051758
Cytoplasmic Foci 0.004089 0.010775 0.000141 0.000832 0.009987 0.008155 0.000204 0.003258 0.006311 0.009003 0.000199 0.001867
Eisosomes 0.000022 0.000056 0.000057 0.000027 0.000034 0.000032 0.000021 0.000038 0.000027 0.000040 0.000024 0.000032
Endoplasmic Reticulum 0.001842 0.006554 0.009030 0.007545 0.001650 0.003454 0.004512 0.008225 0.001770 0.004458 0.004863 0.007835
Endosome 0.000877 0.002685 0.000431 0.001569 0.000597 0.002681 0.000487 0.003830 0.000772 0.002682 0.000483 0.002534
Golgi 0.000336 0.000899 0.000127 0.001382 0.000333 0.001059 0.000252 0.003104 0.000335 0.001007 0.000242 0.002117
Lipid Particles 0.001551 0.002873 0.000433 0.000379 0.002161 0.002249 0.000129 0.001001 0.001780 0.002451 0.000153 0.000644
Mitochondria 0.004130 0.005619 0.001246 0.004247 0.001864 0.006528 0.002770 0.005873 0.003276 0.006234 0.002652 0.004941
Mitotic Spindle 0.004347 0.007972 0.012555 0.026779 0.001001 0.009769 0.007471 0.021839 0.003086 0.009187 0.007865 0.024671
None 0.008714 0.041529 0.001794 0.009189 0.036223 0.011794 0.004135 0.010164 0.019080 0.021421 0.003954 0.009605
Nuclear Periphery 0.002186 0.001321 0.000657 0.003814 0.000961 0.003094 0.001922 0.002437 0.001724 0.002520 0.001823 0.003226
Nuclear Periphery Foci 0.001193 0.002756 0.000288 0.002291 0.001381 0.001011 0.000702 0.001728 0.001264 0.001576 0.000670 0.002051
Nucleolus 0.026119 0.038445 0.015046 0.003538 0.028442 0.026852 0.006411 0.008429 0.026994 0.030605 0.007080 0.005626
Nucleus 0.911552* 0.794393* 0.940135* 0.849888* 0.853451* 0.865862* 0.915015* 0.790076* 0.889659* 0.842724* 0.916963* 0.824364*
Peroxisomes 0.000936 0.003722 0.000469 0.000326 0.000779 0.001879 0.000130 0.000978 0.000877 0.002476 0.000156 0.000604
Vacuole 0.001116 0.004356 0.004572 0.011825 0.001099 0.004923 0.004156 0.024451 0.001109 0.004740 0.004189 0.017213
Vacuole Periphery 0.000429 0.001684 0.000145 0.003660 0.000182 0.001054 0.001138 0.006399 0.000336 0.001258 0.001061 0.004829

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.57 -13.06 -19.44 2.22 6.78 -1.89 -13.74 -12.06 -11.51 -5.11 -8.83 -19.37 -20.82 -8.08 -4.01
Bud Neck -5.15 -7.78 -16.57 -9.04 -8.00 -8.42 -25.13 -15.59 -9.34 8.12 -9.37 -25.30 -22.30 -13.71 6.17
Bud Site -5.10 -3.76 -7.51 -2.50 -4.49 -4.38 -6.75 -7.19 -6.17 -5.19 -5.89 -6.29 -10.12 -7.34 -6.38
Cell Periphery -5.14 -0.90 -3.40 4.15 -2.72 2.35 3.69 0.92 -2.61 -5.58 -2.85 2.35 -1.40 2.21 -5.81
Cytoplasm -6.85 11.51 -1.84 5.82 -13.16 4.32 12.68 -1.42 -5.50 -12.89 -2.66 14.04 -2.80 0.02 -15.54
Cytoplasmic Foci -3.81 7.99 7.15 6.52 -3.41 1.46 9.13 7.03 5.72 -5.23 -2.33 12.02 9.65 9.06 -5.98
Eisosomes -9.45 -8.35 -9.70 5.14 5.22 1.08 4.00 -5.43 -6.85 -11.36 -6.78 -1.80 -10.85 -1.41 -10.06
Endoplasmic Reticulum -10.32 -12.75 -22.62 -4.94 1.76 -6.34 -18.16 -19.10 -14.01 -10.62 -11.28 -21.78 -29.31 -15.68 -12.48
Endosome -2.55 1.01 -1.22 2.22 -3.88 -4.19 1.40 -3.91 0.22 -4.20 -4.59 1.89 -3.84 2.12 -5.61
Golgi -3.61 1.91 -0.72 1.65 -1.43 -1.71 1.34 -1.49 -0.47 -1.70 -2.39 1.81 -1.65 0.25 -2.10
Lipid Particles -2.31 5.65 5.98 4.23 0.07 -0.17 4.19 2.23 3.86 -6.48 -2.08 7.50 4.88 6.44 -8.29
Mitochondria -1.31 2.99 0.56 2.10 -5.09 -4.53 -2.52 -2.85 1.73 -1.56 -3.51 0.40 -1.16 2.84 -2.08
Mitotic Spindle -1.83 -1.20 -7.17 -5.04 -2.09 -4.60 -5.96 -7.96 -4.76 -4.92 -4.03 -4.60 -10.50 -6.75 -6.44
None -7.64 7.50 1.91 8.11 -5.32 8.31 12.12 10.43 3.04 -4.35 -1.00 13.58 9.77 8.36 -5.99
Nuclear Periphery 2.70 3.44 -6.84 -10.14 -10.78 -4.04 -6.85 -9.69 -1.48 -4.71 -2.12 -2.40 -10.12 -4.98 -8.27
Nuclear Periphery Foci -2.57 2.35 -0.13 2.39 -2.38 1.42 2.87 -0.35 -1.79 -3.20 -0.57 3.16 -0.35 0.17 -3.49
Nucleolus -4.01 2.51 16.09 13.89 3.34 0.90 10.03 11.03 10.45 2.35 -1.57 13.16 19.12 17.63 6.19
Nucleus 12.02 -1.10 9.80 -5.25 7.01 -1.56 -7.38 7.63 9.19 14.90 8.14 -3.47 12.73 3.60 15.94
Peroxisomes -4.24 3.01 4.55 5.57 4.15 -2.79 9.19 1.01 2.99 -2.00 -4.59 6.92 3.82 6.25 -2.06
Vacuole -5.12 -2.55 -12.06 -9.70 -4.12 -6.04 -7.13 -13.57 -12.14 -11.88 -7.77 -7.70 -18.05 -15.40 -14.36
Vacuole Periphery -1.54 1.93 -1.53 0.37 -1.94 -3.88 -1.80 -2.99 -1.99 -2.46 -3.01 -1.06 -3.26 -1.28 -2.59
Standard name
Human Ortholog
Description Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cup9

Cup9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cup9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available