Standard name
Human Ortholog
Description Small subunit of the heterodimeric cap binding complex with Sto1p; interacts with Npl3p, possibly to package mRNA for export from the nucleus; may have a role in telomere maintenance; contains an RNA-binding motif
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.12 0 0.05 0.05 0.07 – 0.06 0.06 0 0 0.05 0.07 0.09 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.87 0.88 0.85 0.85 0.87 0.76 0.79 – 0.75 0.71 0.8 0.73 0.8 0.87 0.78 0.82 0.81 0.83 0.77 0.82 0.67 0.66 0.6 0.56
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.42 0.28 0.17 0.33 0.17 0.46 0.33 – 0.49 0.47 0.44 0.51 0.39 0.28 0.34 0.23 0.23 0.19 0.19 0.14 0.28 0.32 0.31 0.31
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3
Bud Neck 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 1 1 4 6 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cytoplasm 1 6 5 3 7 5 14 – 7 10 6 6 8 26 39 13 9 14 0 0 1 0 2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0
Endosome 0 2 0 1 0 1 0 – 0 0 0 0 0 5 5 1 1 0 0 1 0 0 1 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 3 0 0 0 0 1
Mitochondria 3 4 0 1 1 1 0 – 5 7 5 10 1 1 2 4 6 2 0 0 0 0 1 2
Nucleus 179 144 34 85 127 72 167 – 89 115 131 156 122 306 323 282 196 188 168 135 46 142 234 160
Nuclear Periphery 0 0 0 0 0 1 2 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 86 46 7 33 25 44 71 – 58 75 72 108 59 98 142 80 55 44 41 22 19 69 120 90
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 2 7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 1 0 5 6 0 2 0 0 0 0 0 4 3
Vac/Vac Membrane 1 2 0 0 3 0 1 – 2 1 0 0 1 0 1 8 5 5 2 4 0 2 12 21
Unique Cell Count 206 163 40 100 146 95 212 118 161 164 213 153 351 416 342 242 226 220 166 69 217 394 288
Labelled Cell Count 270 204 46 123 163 124 255 163 211 218 287 191 443 527 392 275 256 220 166 69 217 394 288


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.0 21.0 18.0 15.9 15.9 10.2 14.3 – 12.3 12.2 12.2 11.7 21.0 17.9 21.7 20.1 21.2 22.1 20.0 21.6 21.4
Std Deviation (1e-4) 5.0 7.2 6.3 6.1 4.2 3.3 5.0 – 3.9 4.1 5.0 3.8 7.1 7.2 9.5 8.1 8.1 7.6 6.7 7.1 7.4
Intensity Change (Log2) – – – -0.18 -0.18 -0.82 -0.33 – -0.56 -0.56 -0.56 -0.62 0.22 -0.01 0.27 0.16 0.23 0.29 0.15 0.26 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.3 -1.2 -0.9 – -1.3 -1.8 -0.7 -1.6 -0.8 0.4 -1.1 -0.4 -0.6 -0.3
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.8 -0.1 3.2 2.0 – 3.5 3.3 3.1 3.9 2.5 1.4 2.1 0.8 0.7 0.3
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.2613 13.6549 12.1403 11.8711 12.3695 12.125 17.0886 17.4024 16.8407 13.9573 13.5022 16.2509 18.6563 20.3956 18.6559 18.7905 15.6459 17.4641
Actin 0.0022 0.001 0.0072 0.0022 0.0001 0.0002 0.002 0.0095 0.0015 0.0331 0.0222 0.0013 0.0122 0 0.0004 0 0.0156 0.0011
Bud 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0008 0.0012 0.0001 0.0002 0 0.0001 0 0.0031 0.0001
Bud Neck 0.0006 0.0001 0.0001 0.0001 0.0003 0.0003 0.0013 0.0001 0.0005 0.0039 0.0003 0.0004 0.0002 0 0 0 0.0005 0.0002
Bud Periphery 0.0002 0 0.0001 0.0001 0.0002 0.0003 0.0002 0 0.0004 0.0014 0.004 0.0002 0.0003 0 0.0001 0 0.009 0.0001
Bud Site 0.001 0.0001 0.0003 0.0004 0.0001 0.0002 0.0052 0.0012 0.0008 0.0118 0.0012 0.0002 0.0008 0 0.0001 0 0.0116 0.0001
Cell Periphery 0.0002 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0003 0.0007 0.0005 0.0001 0.0002 0 0 0 0.0005 0
Cytoplasm 0.0012 0 0.0003 0.0011 0 0.0003 0.0035 0 0.0044 0.0006 0.0002 0 0.0058 0 0.0012 0 0.0006 0.0001
Cytoplasmic Foci 0.0215 0.0021 0.0045 0.0064 0.0018 0.0012 0.0047 0.0004 0.0065 0.0044 0.0029 0.0009 0.0173 0 0.0011 0 0.0077 0.0001
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0005 0.0007 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0009 0 0.0001 0.0009 0 0.0001 0.0009 0 0.0017 0.0018 0.0035 0 0.0064 0 0.0003 0 0.0008 0.0006
Endosome 0.01 0.0011 0.0052 0.0046 0.0003 0.0024 0.0051 0.0001 0.0032 0.0289 0.0037 0.0009 0.0083 0 0.004 0 0.0032 0.0029
Golgi 0.0035 0.0013 0.001 0.0021 0.0004 0.0016 0.0012 0.0003 0.0008 0.0065 0.0031 0.0011 0.0019 0 0.0003 0 0.0022 0.0009
Lipid Particles 0.0116 0.0034 0.0036 0.0051 0.0035 0.0012 0.0132 0.0001 0.0036 0.0092 0.0036 0.0009 0.0085 0 0.0003 0 0.017 0.0001
Mitochondria 0.0039 0.0006 0.0023 0.0005 0.045 0.0021 0.0016 0.0005 0.0054 0.0132 0.0526 0.0124 0.0017 0.0001 0.0003 0.0001 0.008 0.003
None 0.0044 0 0.0004 0.0013 0.0001 0.0008 0.0132 0 0.0085 0.0009 0.0008 0 0.0071 0 0.0005 0 0.0006 0.0001
Nuclear Periphery 0.0077 0.0006 0.0013 0.0106 0.0004 0.0005 0.0087 0.0002 0.0052 0.0052 0.0045 0.0002 0.0081 0.0001 0.0006 0 0.002 0.001
Nucleolus 0.1355 0.0555 0.0563 0.0188 0.5177 0.2456 0.0594 0.0303 0.0313 0.0402 0.3827 0.2076 0.0841 0.0298 0.0313 0.0258 0.2559 0.1413
Nucleus 0.7758 0.9297 0.9078 0.9297 0.4235 0.741 0.8688 0.9565 0.9228 0.8205 0.5039 0.7721 0.8076 0.9699 0.9583 0.974 0.6328 0.8479
Peroxisomes 0.0029 0.0034 0.0014 0.0075 0.0009 0.0002 0.0068 0.0006 0.0005 0.0057 0.0033 0.0008 0.008 0 0.0001 0 0.0222 0
Punctate Nuclear 0.0134 0.0008 0.0039 0.0078 0.0044 0.0013 0.0019 0.0001 0.0009 0.0052 0.0007 0.0003 0.019 0 0.0007 0 0.0013 0.0001
Vacuole 0.0014 0.0001 0.0018 0.0003 0.0004 0.0004 0.0011 0 0.0008 0.003 0.0015 0.0002 0.001 0 0.0002 0 0.0029 0.0001
Vacuole Periphery 0.0017 0.0001 0.0024 0.0004 0.0006 0.0003 0.0006 0 0.0006 0.0026 0.003 0.0002 0.001 0 0.0001 0 0.0023 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.5543 83.3779 42.0249 71.9373 74.1545 52.7652 69.59 61.1933 58.2809 68.4863
Translational Efficiency 2.1006 1.464 2.4559 1.2834 1.5353 2.1736 1.4831 1.5135 1.7661 1.5956

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
957 876 1917 1281 1482 2476 178 1296 2439 3352 2095 2577

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1195.24 1280.64 1931.61 2029.62 1281.89 1588.73 2041.37 1947.66 1247.89 1508.21 1940.94 1988.40
Standard Deviation 288.28 378.47 369.93 432.30 301.09 335.04 365.06 500.79 299.14 372.39 370.78 469.79
Intensity Change Log 2 — 0.099565 0.692504 0.763909 — 0.309601 0.671265 0.603469 — 0.212064 0.681552 0.683113

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000546 0.000564 0.000627 0.000652 0.000444 0.000489 0.000990 0.000583 0.000484 0.000509 0.000658 0.000617
Bud Neck 0.002173 0.002842 0.009706 0.007804 0.001862 0.004973 0.007050 0.006734 0.001984 0.004416 0.009480 0.007266
Bud Site 0.001535 0.000464 0.000736 0.001391 0.000223 0.000561 0.001071 0.000725 0.000738 0.000536 0.000765 0.001056
Cell Periphery 0.000173 0.000101 0.000029 0.000073 0.000032 0.000131 0.000058 0.000085 0.000087 0.000123 0.000032 0.000079
Cytoplasm 0.001509 0.001894 0.000042 0.000175 0.000109 0.000232 0.000046 0.000289 0.000659 0.000666 0.000043 0.000232
Cytoplasmic Foci 0.000599 0.000395 0.000116 0.000615 0.000167 0.000231 0.000466 0.000504 0.000337 0.000274 0.000146 0.000559
Eisosomes 0.000153 0.000110 0.000142 0.000087 0.000137 0.000204 0.000176 0.000071 0.000143 0.000179 0.000145 0.000079
Endoplasmic Reticulum 0.000549 0.000454 0.000262 0.000239 0.000375 0.000471 0.000334 0.000239 0.000443 0.000467 0.000268 0.000239
Endosome 0.000473 0.000026 0.000009 0.000231 0.000023 0.000119 0.000022 0.000210 0.000200 0.000094 0.000010 0.000221
Golgi 0.000198 0.000053 0.000040 0.000071 0.000047 0.000064 0.000070 0.000164 0.000106 0.000061 0.000043 0.000118
Lipid Particles 0.000173 0.000009 0.000004 0.000122 0.000015 0.000326 0.000014 0.000036 0.000077 0.000243 0.000005 0.000079
Mitochondria 0.000866 0.001324 0.001416 0.001815 0.000713 0.001044 0.002261 0.001755 0.000773 0.001117 0.001488 0.001785
Mitotic Spindle 0.000224 0.000110 0.000227 0.001716 0.000036 0.000821 0.005406 0.000666 0.000110 0.000635 0.000667 0.001188
None 0.001473 0.004028 0.000454 0.000791 0.000750 0.000922 0.000603 0.001191 0.001034 0.001733 0.000467 0.000992
Nuclear Periphery 0.000030 0.000036 0.000036 0.000128 0.000017 0.000074 0.000054 0.000151 0.000022 0.000064 0.000038 0.000140
Nuclear Periphery Foci 0.000339 0.000048 0.000013 0.000122 0.000249 0.000108 0.000011 0.000085 0.000284 0.000092 0.000013 0.000103
Nucleolus 0.087528 0.083760 0.059784 0.039693 0.073439 0.072310 0.086761 0.033240 0.078967 0.075302 0.062076 0.036448
Nucleus 0.896231* 0.898514* 0.922006* 0.941514* 0.917724* 0.914140* 0.879977* 0.951134* 0.909290* 0.910057* 0.918435* 0.946352*
Peroxisomes 0.004289 0.003120 0.004017 0.002195 0.003060 0.001613 0.014086 0.001436 0.003542 0.002007 0.004872 0.001813
Vacuole 0.000652 0.001054 0.000138 0.000361 0.000261 0.000653 0.000331 0.000314 0.000414 0.000758 0.000154 0.000337
Vacuole Periphery 0.000289 0.001094 0.000194 0.000204 0.000317 0.000511 0.000213 0.000389 0.000306 0.000664 0.000196 0.000297

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.68 -4.95 -3.98 -3.07 -0.12 -2.12 -7.25 -4.30 -2.99 5.07 -1.52 -10.74 -6.31 -5.07 2.46
Bud Neck -2.31 -27.49 -8.38 -6.73 2.30 -13.40 -7.90 -10.03 -3.86 0.57 -12.67 -30.76 -12.74 -6.84 4.28
Bud Site 1.00 0.70 -0.04 -2.13 -1.56 -4.43 -2.07 -3.23 -1.23 0.96 0.44 -0.35 -0.97 -2.41 -1.21
Cell Periphery 0.82 1.69 1.10 0.91 -2.56 -3.14 -0.81 -2.97 1.23 -0.07 -0.91 1.54 0.13 1.54 -3.34
Cytoplasm -0.28 2.34 2.09 1.47 -2.79 -1.06 0.66 -1.68 0.20 -2.40 -0.22 2.45 1.60 1.45 -3.67
Cytoplasmic Foci 0.46 1.88 -0.27 -0.76 -2.21 -0.47 -1.55 -0.98 -0.73 0.15 0.25 1.37 -1.10 -1.30 -2.06
Eisosomes 2.82 0.46 4.58 2.88 8.33 -2.50 -2.80 5.51 5.26 8.40 -1.80 -0.58 7.05 5.37 10.79
Endoplasmic Reticulum 1.17 3.89 4.33 4.62 1.95 -2.14 0.56 4.54 5.16 3.31 -0.58 4.96 6.15 6.60 2.54
Endosome 1.26 1.30 0.60 -1.70 -1.83 -2.27 -0.28 -1.15 -0.51 -1.10 0.73 1.35 -0.14 -1.21 -2.10
Golgi 1.04 1.09 0.85 -1.28 -1.71 -0.46 -1.05 -0.92 -0.79 -0.69 0.76 1.03 -0.16 -0.91 -1.17
Lipid Particles 1.15 1.17 0.41 -1.61 -1.66 -1.03 0.01 -0.74 0.96 -0.79 -0.65 1.25 0.11 0.70 -1.94
Mitochondria -3.17 -6.16 -7.39 -3.81 -3.37 -2.18 -3.25 -5.34 -3.06 1.20 -2.83 -10.59 -8.81 -4.56 -1.92
Mitotic Spindle 0.45 -0.14 -1.78 -1.95 -1.76 -2.24 -1.09 -2.13 0.03 0.93 -1.95 -1.31 -2.55 -1.22 -0.91
None -3.53 4.04 2.16 4.56 -1.64 -0.99 1.05 -2.29 -1.19 -2.94 -2.92 4.75 0.12 2.85 -3.74
Nuclear Periphery -0.94 -1.62 -3.70 -3.41 -3.34 -3.51 -1.55 -3.46 -1.92 -1.73 -3.48 -3.81 -5.03 -3.02 -4.15
Nuclear Periphery Foci 1.48 1.68 1.03 -1.22 -1.86 0.66 1.13 0.80 0.34 -1.26 1.22 1.81 1.17 -0.14 -2.20
Nucleolus 0.62 5.36 9.91 8.62 5.91 0.28 -1.84 9.97 11.19 6.28 1.11 4.08 13.70 13.66 8.98
Nucleus -0.29 -4.08 -7.48 -6.93 -4.71 0.90 3.68 -6.92 -8.65 -6.40 -0.13 -1.28 -9.74 -10.34 -8.28
Peroxisomes 2.89 -0.44 5.34 2.77 6.09 5.59 -6.33 5.62 0.48 7.25 7.02 -4.64 7.32 0.69 9.77
Vacuole -1.11 2.63 1.17 2.02 -2.38 -2.48 -1.14 -0.63 2.06 0.53 -2.16 3.09 0.60 2.67 -2.97
Vacuole Periphery -3.22 1.77 1.40 3.59 -0.69 -2.15 1.31 -0.49 1.03 -1.38 -3.97 2.60 0.17 3.83 -1.83
Standard name
Human Ortholog
Description Small subunit of the heterodimeric cap binding complex with Sto1p; interacts with Npl3p, possibly to package mRNA for export from the nucleus; may have a role in telomere maintenance; contains an RNA-binding motif
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Cbc2

Cbc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available