Standard name
Human Ortholog
Description Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.07 0.13 0.25 0.27 0.36 0.46 0.47 0.39 0.39 0.38 0.4 0.09 0 0.12 0.09 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0 0.27 0.2 0.16 0.13 0.12 0.13 0.07 0 0 0.06 0.16 0.31 0.24 0.2 0.16 0.14 0.28 0.13 0.27 0.26 0.37 0.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.49 0.09 0.2 0.13 0.31 0.35 0.22 0.43 0.54 0.62 0.49 0.17 0.09 0.08 0 0.05 0.05 0 0.06 0.07 0 0 0
Nucleus 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0.16 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0.07 0 0.07 0.07
Nucleolus 0.16 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0.06 0.06 0 0 0 0 0 0.05 0.06
Vac/Vac Membrane 0.59 0.62 0.62 0.72 0.64 0.46 0.33 0.4 0.35 0.3 0.28 0.29 0.71 0.7 0.64 0.73 0.81 0.84 0.49 0.72 0.48 0.61 0.45 0.39
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 2 5 2 1 0 8 5 6 12 13 8 1 2 3 1 0 0 1 1 1 1 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 1 1 2 0 0 1 0 0 0 2 0 1 0 0 0 0 0 0
Cytoplasm 6 19 31 54 91 93 204 203 125 139 106 109 18 17 43 42 38 21 0 2 9 2 0 2
Endoplasmic Reticulum 3 3 0 0 1 3 6 1 1 1 2 1 0 2 3 18 9 12 0 0 1 0 2 1
Endosome 11 3 61 44 55 34 53 57 24 11 12 15 34 125 83 98 53 40 38 41 68 54 131 148
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 7 9 4 1 0 0 1 1 3
Mitochondria 43 144 20 42 43 82 154 98 139 193 172 134 36 35 29 22 17 15 5 18 17 3 9 7
Nucleus 4 22 7 3 7 5 5 9 6 9 3 5 6 5 3 24 6 3 0 4 6 4 3 2
Nuclear Periphery 20 9 3 2 0 0 0 0 0 0 1 0 4 7 8 10 3 6 16 14 16 7 23 24
Nucleolus 19 3 5 3 2 0 0 0 0 0 1 0 11 33 29 26 8 8 1 0 0 0 2 2
Peroxisomes 0 0 1 0 0 0 0 1 0 0 0 0 1 9 3 1 1 2 0 0 1 0 0 0
SpindlePole 2 3 24 2 5 1 2 2 1 1 2 1 8 42 22 28 19 11 0 1 2 6 17 22
Vac/Vac Membrane 72 180 143 155 214 119 147 174 111 107 78 78 147 276 227 360 266 234 67 232 121 129 158 141
Unique Cell Count 122 291 230 215 337 261 443 436 321 359 277 271 207 397 352 493 328 278 136 323 253 212 357 367
Labelled Cell Count 180 388 300 307 419 338 581 553 415 473 391 352 266 553 453 639 429 357 136 323 253 212 357 367


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 4.9 6.4 6.0 6.5 5.3 5.3 6.0 5.2 4.8 4.6 5.0 5.9 6.1 6.1 8.0 8.5 8.1 5.7 5.6 6.5
Std Deviation (1e-4) 1.0 0.9 1.3 1.4 1.5 1.4 1.3 1.5 1.4 1.3 1.3 0.9 1.1 1.4 1.5 1.6 1.5 1.5 1.4 1.0 1.8
Intensity Change (Log2) – – – -0.09 0.02 -0.26 -0.27 -0.1 -0.31 -0.42 -0.48 -0.35 -0.12 -0.07 -0.06 0.32 0.41 0.34 -0.17 -0.18 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.0 5.0 5.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2184 3.6851 3.6902 3.409 2.9701 3.6795 1.4756 1.8458 1.4656 1.1772 1.358 1.4339 1.1703 1.454 1.055 1.1753 1.1967 0.8706
Actin 0.0065 0.0001 0.0042 0.0412 0 0.0003 0.0077 0.0001 0.005 0.0005 0.0017 0.0006 0.0234 0 0.0112 0.0583 0.0552 0
Bud 0.0003 0.0004 0.001 0.0007 0.0005 0.0005 0.0006 0.0007 0.0005 0.0001 0.0007 0.0002 0.0003 0.0003 0.0005 0.0012 0.0011 0.0003
Bud Neck 0.0005 0.0003 0.0012 0.0008 0.0002 0.0006 0.001 0.0005 0.0006 0.0004 0.0008 0.0002 0.0008 0.0003 0.0007 0.0026 0.0015 0.0004
Bud Periphery 0.0006 0.0013 0.0044 0.0017 0.0013 0.0029 0.0013 0.0008 0.0009 0.0003 0.0009 0.0004 0.0005 0.0004 0.0008 0.0014 0.001 0.0012
Bud Site 0.0007 0.0004 0.0023 0.0014 0.0004 0.0003 0.0035 0.0025 0.0012 0.0002 0.0013 0.0003 0.0016 0.0028 0.0019 0.0137 0.0119 0.0003
Cell Periphery 0.0006 0.0005 0.0007 0.0006 0.0003 0.0004 0.0005 0.0003 0.0004 0.0002 0.0002 0.0002 0.0006 0.0003 0.0004 0.0005 0.0001 0.0002
Cytoplasm 0.008 0.0098 0.0078 0.0018 0.0031 0.0029 0.0056 0.0082 0.0046 0.0009 0.0109 0.0018 0.0058 0.0066 0.0051 0.0052 0.0046 0.0012
Cytoplasmic Foci 0.0377 0.017 0.0367 0.0203 0.0178 0.0135 0.0253 0.0294 0.0253 0.0069 0.0398 0.0113 0.0514 0.0401 0.0442 0.0561 0.0713 0.034
Eisosomes 0.0002 0.0001 0.0001 0.0003 0 0 0.0001 0 0.0001 0 0 0 0.0002 0 0.0002 0.0005 0.0002 0
Endoplasmic Reticulum 0.0043 0.0044 0.0033 0.0019 0.0022 0.0044 0.0036 0.003 0.0031 0.0008 0.0027 0.0017 0.0046 0.002 0.0023 0.0015 0.0006 0.0025
Endosome 0.3958 0.3958 0.4214 0.4354 0.4608 0.3249 0.4023 0.3334 0.3984 0.3152 0.296 0.3166 0.3472 0.3291 0.3745 0.2838 0.3281 0.2611
Golgi 0.0176 0.0146 0.0259 0.0195 0.0164 0.0157 0.0316 0.0135 0.0247 0.0249 0.0181 0.0157 0.02 0.0123 0.0257 0.0138 0.0184 0.0117
Lipid Particles 0.0764 0.0205 0.0601 0.0604 0.0166 0.0429 0.0363 0.0112 0.0161 0.0089 0.0111 0.0115 0.0764 0.0376 0.0518 0.0824 0.0651 0.0714
Mitochondria 0.0283 0.0569 0.042 0.0228 0.0134 0.0221 0.0354 0.0118 0.0467 0.0193 0.0205 0.0181 0.0261 0.0181 0.0479 0.01 0.0027 0.009
None 0.0036 0.0222 0.0035 0.001 0.0005 0.0092 0.0082 0.0014 0.0007 0.0001 0.0007 0.0004 0.0101 0.0007 0.0014 0.0034 0.0011 0.0002
Nuclear Periphery 0.019 0.0134 0.0073 0.0111 0.0064 0.0216 0.0226 0.0139 0.0121 0.0023 0.0068 0.0119 0.0222 0.0178 0.0074 0.0044 0.002 0.0228
Nucleolus 0.0053 0.0008 0.0047 0.0016 0.0009 0.0016 0.003 0.0008 0.001 0.0003 0.0003 0.0008 0.0056 0.0074 0.0009 0.0065 0.0021 0.0078
Nucleus 0.0023 0.0017 0.0056 0.0012 0.001 0.0038 0.0063 0.0018 0.0021 0.0004 0.0007 0.001 0.0059 0.0033 0.001 0.0021 0.0014 0.0055
Peroxisomes 0.0091 0.0015 0.0127 0.0041 0.0024 0.0075 0.0082 0.0059 0.0059 0.001 0.0103 0.0014 0.0116 0.0018 0.0106 0.0375 0.0295 0.0031
Punctate Nuclear 0.0058 0.0003 0.0022 0.0005 0.0002 0.001 0.0019 0.0003 0.0007 0 0.0006 0.0002 0.0047 0.015 0.0009 0.0054 0.0283 0.0015
Vacuole 0.2148 0.2109 0.1926 0.2397 0.2254 0.2382 0.2154 0.2818 0.2171 0.2936 0.2691 0.2273 0.2069 0.263 0.2223 0.2634 0.1931 0.2021
Vacuole Periphery 0.1625 0.2272 0.1605 0.132 0.2301 0.2857 0.1794 0.2787 0.2327 0.3237 0.3068 0.3786 0.1741 0.2412 0.1884 0.1463 0.1805 0.3637

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8158 8.2198 10.674 14.2708 9.8355 8.271 12.3627 11.7797 15.0063 16.0589
Translational Efficiency 2.0075 1.8659 1.2449 1.0542 1.6339 2.0392 1.2053 1.3997 1.1134 1.2213

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1760 1166 161 124 2058 1429 235 1816 3818 2595 396 1940

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 676.39 835.20 1096.68 1073.40 773.61 845.37 1035.84 978.59 728.79 840.80 1060.58 984.65
Standard Deviation 73.23 118.73 110.84 148.71 87.73 118.21 102.58 127.29 94.71 118.55 110.15 130.84
Intensity Change Log 2 — 0.304266 0.697215 0.666261 — 0.127976 0.421123 0.339098 — 0.212898 0.556506 0.500971

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000049 0.000766 0.000218 0.000039 0.000193 0.000254 0.000039 0.000059 0.000129 0.000462 0.000050
Bud Neck 0.012978 0.003263 0.019006 0.010695 0.001863 0.003021 0.013614 0.004136 0.006987 0.003130 0.015806 0.004556
Bud Site 0.010854 0.007496 0.022114 0.020522 0.003798 0.008757 0.011236 0.014143 0.007051 0.008191 0.015658 0.014551
Cell Periphery 0.000621 0.000112 0.000106 0.000064 0.000349 0.000204 0.000061 0.000074 0.000474 0.000163 0.000079 0.000073
Cytoplasm 0.032330 0.012384 0.005706 0.015557 0.004358 0.004959 0.004950 0.015568 0.017252 0.008295 0.005257 0.015567
Cytoplasmic Foci 0.089375 0.056086 0.014966 0.033427 0.023186 0.039862 0.011587 0.018766 0.053697 0.047152 0.012961 0.019704
Eisosomes 0.000093 0.000018 0.000047 0.000020 0.000018 0.000020 0.000038 0.000007 0.000053 0.000019 0.000041 0.000008
Endoplasmic Reticulum 0.015170 0.001750 0.004697 0.001521 0.007428 0.002251 0.003854 0.000680 0.010997 0.002026 0.004196 0.000734
Endosome 0.181414 0.213856* 0.069107 0.159395 0.085345 0.196926 0.106521 0.095559 0.129631 0.204533* 0.091310 0.099639
Golgi 0.021336 0.022277 0.008525 0.015256 0.011035 0.027368 0.003761 0.018516 0.015784 0.025080 0.005698 0.018307
Lipid Particles 0.011657 0.002403 0.001479 0.001815 0.001973 0.002131 0.002062 0.000284 0.006437 0.002253 0.001825 0.000382
Mitochondria 0.103835* 0.021267 0.020700 0.001391 0.043120 0.040285 0.003492 0.007626 0.071108 0.031740 0.010488 0.007227
Mitotic Spindle 0.000593 0.004186 0.088229 0.025278 0.000135 0.004222 0.011182 0.009962 0.000346 0.004205 0.042506 0.010941
None 0.000775 0.000502 0.000230 0.000071 0.000125 0.000169 0.000180 0.000098 0.000425 0.000318 0.000200 0.000096
Nuclear Periphery 0.000623 0.000701 0.016793 0.003868 0.001182 0.000637 0.008717 0.000799 0.000924 0.000666 0.012001 0.000995
Nuclear Periphery Foci 0.000301 0.002530 0.009162 0.011089 0.000443 0.001927 0.004510 0.001219 0.000378 0.002198 0.006401 0.001850
Nucleolus 0.000674 0.000773 0.016621 0.008308 0.000453 0.000511 0.023811 0.001583 0.000555 0.000629 0.020888 0.002013
Nucleus 0.003715 0.001721 0.048084 0.016933 0.001224 0.000809 0.034981 0.002707 0.002372 0.001219 0.040308 0.003617
Peroxisomes 0.002447 0.003275 0.009361 0.007183 0.000424 0.003225 0.005933 0.001660 0.001356 0.003247 0.007327 0.002013
Vacuole 0.273629* 0.331603* 0.377982* 0.543908* 0.397526* 0.335318* 0.583109* 0.596577* 0.340413* 0.333649* 0.499711* 0.593211*
Vacuole Periphery 0.237499* 0.313748* 0.266319* 0.123479* 0.415976* 0.327207* 0.166149* 0.209996* 0.333702* 0.321159* 0.206875* 0.204466*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.42 -3.28 -1.55 -1.93 2.56 -1.90 -4.43 -0.14 1.92 4.67 -1.55 -4.38 0.41 1.71 4.45
Bud Neck 7.32 -2.44 0.88 -3.32 2.71 -1.88 -7.83 -6.08 -2.31 5.97 5.48 -6.24 2.81 -3.35 7.78
Bud Site 3.22 -3.01 -2.00 -2.69 0.32 -4.26 -3.40 -8.39 -3.62 -0.96 -1.44 -4.30 -6.37 -5.03 0.55
Cell Periphery 10.08 9.90 10.92 2.67 2.05 2.69 5.84 5.54 5.34 -1.09 8.33 11.06 11.20 5.61 0.68
Cytoplasm 7.82 10.55 3.15 -0.87 -2.19 -0.77 -0.73 -9.18 -8.42 -5.75 7.04 7.41 -0.76 -6.27 -6.86
Cytoplasmic Foci 8.41 21.65 7.86 3.28 -2.61 -6.90 6.14 2.86 8.97 -4.04 2.76 21.00 18.48 13.84 -4.34
Eisosomes 13.70 5.12 11.30 -0.36 3.93 -0.73 -4.41 5.88 5.27 7.20 10.81 1.91 17.11 6.56 9.45
Endoplasmic Reticulum 12.77 6.36 12.59 0.40 3.25 7.93 4.38 11.44 5.30 5.84 14.88 8.03 17.72 6.78 6.66
Endosome -3.94 14.82 1.46 3.12 -4.69 -16.69 -2.20 -2.02 15.13 1.22 -14.16 6.37 7.73 19.22 -1.25
Golgi -0.48 6.48 0.91 1.03 -0.83 -8.62 6.69 -4.47 4.40 -9.85 -6.80 9.54 -1.34 4.22 -8.17
Lipid Particles 8.86 10.05 8.37 0.54 -0.35 -0.46 -0.28 6.50 5.19 4.41 7.67 8.45 13.52 6.00 4.96
Mitochondria 18.79* 13.81 25.29 11.31 4.17 0.74 13.27 12.58 11.03 -2.34 13.31 19.30 24.85 12.48 1.41
Mitotic Spindle -3.60 -5.76 -2.69 -2.28 3.48 -4.32 -3.42 -6.64 -3.54 0.43 -5.61 -6.32 -7.03 -4.26 4.49
None 0.69 1.85 2.42 1.62 5.13 -1.65 -1.03 1.40 2.62 1.40 0.58 1.57 2.42 1.82 2.90
Nuclear Periphery -0.51 -3.76 -2.24 -2.18 2.89 1.25 -2.29 0.72 -1.74 2.41 1.04 -4.27 -0.52 -2.58 4.23
Nuclear Periphery Foci -2.56 -4.09 -2.28 -1.81 -0.47 -2.99 -3.04 -2.11 1.08 2.34 -3.83 -5.07 -3.30 0.36 3.49
Nucleolus -0.73 -5.20 -1.91 -1.88 1.86 -0.33 -5.52 -3.87 -4.31 5.22 -0.63 -7.27 -4.39 -4.23 6.68
Nucleus 3.36 -4.78 -2.35 -2.69 3.06 1.23 -5.17 -3.44 -4.39 4.85 3.52 -6.98 -2.66 -4.55 6.64
Peroxisomes -1.21 -1.83 -1.92 -1.50 0.57 -4.25 -3.43 -2.41 1.82 2.49 -3.97 -3.27 -1.29 1.76 2.80
Vacuole -6.79 -5.78 -11.04 -8.69 -5.82 7.88 -11.48 -26.84 -32.92 -0.82 1.18 -12.15 -38.75 -38.48 -6.95
Vacuole Periphery -8.46 -1.03 9.02 13.71 6.34 10.62 20.83 30.82* 15.82 -3.71 2.05 12.29 23.46 19.16 0.07
Standard name
Human Ortholog
Description Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin
Localization
Cell Percentages vacuolar membrane (57%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Tco89

Tco89


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tco89-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available