Standard name
Human Ortholog
Description Mitochondrial NADH kinase; phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.07 0.07 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0 0 0 0 0 0 0 0 0 0.12 0.21 0.18 0.15 0.1 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 0 0 0 0 0 0 0.22 0.21 0.2 0.17 0.2 0.31 0.09 0.07 0.07 0.07 0.07 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.13 0.19 0.15 0.1 0.07 0 0 0.06 0.14 0.09
Mitochondria 0.97 0.93 0.87 0.92 0.87 0.93 0.96 0.93 0.95 0.96 0.94 0.96 0.44 0.21 0.27 0.14 0.08 0.09 0.73 0.71 0.74 0.72 0.63 0.64
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 0.06 0.05 0.08 0.05 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.12 0.13 0.11 0 0 0 0 0 0 0 0.16 0.06 0.06 0.14 0.25 0.38 0.06 0 0.11 0.05 0.06 0.08
SpindlePole 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0.1 0.15 0.07 0.18 0.18 0.17 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0.08 0 0 0 0 0 0 0 0.12 0.15 0.11 0.13 0.16 0.08 0 0.05 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 0 1 0 1 0 0 0 2 1 1 1 0 0 1 1 1 1 3 8
Bud 3 1 8 11 13 11 9 8 15 8 6 3 2 2 0 4 5 2 0 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1 2 0 0 0 0
Bud Site 0 2 1 0 2 4 5 4 5 5 3 8 0 0 0 0 3 1 – – – – – –
Cell Periphery 2 0 6 7 3 1 2 2 2 3 1 4 6 10 2 8 3 3 0 0 0 0 0 0
Cytoplasm 3 8 6 4 5 6 8 6 4 5 6 1 22 29 19 21 10 10 0 1 0 1 0 2
Endoplasmic Reticulum 2 5 0 0 1 0 1 4 1 2 0 2 6 9 8 3 4 5 0 0 0 0 0 2
Endosome 0 0 8 5 17 2 2 1 1 0 0 0 39 29 22 24 20 27 16 7 16 12 12 12
Golgi 0 1 0 1 0 0 0 0 0 0 0 1 16 13 14 27 15 9 11 3 8 10 23 21
Mitochondria 172 97 196 155 171 255 275 238 267 278 221 281 78 29 29 20 8 8 128 73 178 127 107 154
Nucleus 1 0 1 0 4 6 7 3 1 3 2 0 2 1 0 3 1 1 0 0 0 0 1 2
Nuclear Periphery 7 0 4 1 1 15 18 13 22 15 12 29 0 1 0 3 1 0 0 0 0 0 0 2
Nucleolus 5 0 5 1 5 11 10 12 9 13 12 20 0 0 0 3 1 1 0 0 0 0 0 0
Peroxisomes 0 0 26 22 21 2 3 8 1 1 3 5 29 9 7 20 26 33 11 3 26 9 10 20
SpindlePole 3 1 12 8 7 7 5 5 6 3 3 5 17 21 8 25 18 15 0 1 2 2 1 1
Vac/Vac Membrane 13 2 7 2 16 8 3 8 9 4 5 6 22 21 12 18 16 7 2 5 0 7 7 6
Unique Cell Count 178 104 226 168 197 273 286 255 282 290 236 294 176 141 108 139 102 88 177 103 241 176 171 240
Labelled Cell Count 212 118 280 217 266 328 350 312 344 340 274 365 242 175 122 180 131 123 177 103 241 176 171 240


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.8 5.1 5.1 5.0 4.3 4.3 4.1 4.0 3.9 3.9 3.9 6.6 6.7 6.2 7.0 7.4 7.4 7.2 7.5 8.3
Std Deviation (1e-4) 0.8 1.0 0.9 1.4 1.0 1.4 1.4 1.2 0.9 1.0 1.3 1.0 1.2 1.6 1.3 2.0 2.4 2.0 1.3 1.4 2.5
Intensity Change (Log2) – – – 0.02 -0.04 -0.23 -0.25 -0.32 -0.35 -0.36 -0.39 -0.37 0.38 0.4 0.29 0.47 0.54 0.53 0.5 0.57 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.6 4.1 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.5589 5.3932 5.4033 4.6884 4.1318 5.6678 4.9833 5.9712 5.7416 5.7055 4.6587 5.4593 4.8783 4.9665 4.2953 5.1231 4.8006 4.8142
Actin 0.0052 0.0026 0.0008 0.0016 0.0025 0.0004 0.0385 0.0146 0.0198 0.0003 0.0075 0.0047 0.0252 0.0066 0.0168 0.0006 0.0356 0.0019
Bud 0 0 0.0001 0.0002 0.0001 0 0.0004 0.0002 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0.0004 0.0001
Bud Neck 0.0001 0 0 0 0.0001 0 0.0008 0.0001 0.0001 0 0.0002 0.0002 0.0017 0.0001 0.0001 0 0.0003 0.0002
Bud Periphery 0.0001 0.0001 0.0002 0.0005 0.0002 0 0.0008 0.0003 0.0002 0.0003 0.0006 0.0002 0.0006 0.0003 0.0001 0.0003 0.0009 0.0002
Bud Site 0.0001 0.0001 0.0001 0.0001 0.0005 0 0.0023 0.001 0.0003 0 0.0005 0.0001 0.0032 0.0006 0.0003 0.0001 0.0006 0.0001
Cell Periphery 0.0001 0.0001 0.0005 0 0.0001 0 0.0003 0.0001 0.0001 0.0002 0.0004 0 0.0003 0.0001 0 0 0.0001 0.0001
Cytoplasm 0 0 0 0.0001 0.0004 0 0.0022 0.0001 0.0001 0 0.0001 0.0005 0.0158 0 0.0002 0 0.0001 0
Cytoplasmic Foci 0.0018 0.0042 0.0016 0.0025 0.0056 0.0005 0.028 0.0036 0.0029 0.0002 0.0014 0.003 0.0153 0.0032 0.0157 0.0003 0.0009 0.0018
Eisosomes 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0011 0.0008 0.0002 0.0001 0.0002 0.0001 0.0005 0.0002 0.0002 0.0001 0.0003 0.0002
Endoplasmic Reticulum 0.0002 0 0 0 0.0009 0 0.0024 0 0.0004 0.0001 0.0005 0.001 0.0017 0.0001 0.0002 0 0.0006 0
Endosome 0.0037 0.0096 0.0019 0.0062 0.0107 0.0016 0.0377 0.0079 0.0045 0.0035 0.0071 0.0113 0.0181 0.0056 0.0068 0.0018 0.006 0.0044
Golgi 0.0119 0.0313 0.0064 0.048 0.0183 0.0057 0.0267 0.0292 0.011 0.0032 0.0107 0.0163 0.0304 0.0107 0.0144 0.0025 0.011 0.0188
Lipid Particles 0.0146 0.0269 0.008 0.0125 0.013 0.0047 0.0455 0.0056 0.0159 0.0007 0.0084 0.0102 0.0281 0.0185 0.0204 0.0005 0.0031 0.0139
Mitochondria 0.9304 0.9078 0.9645 0.9165 0.9258 0.9769 0.7435 0.9158 0.9286 0.9668 0.9472 0.9273 0.8066 0.9267 0.8749 0.9867 0.929 0.9506
None 0.0001 0 0 0 0.0008 0 0.0008 0 0.0001 0 0.0001 0.0001 0.0031 0 0.0003 0 0.0002 0
Nuclear Periphery 0.0003 0 0 0 0.0079 0 0.0053 0 0.0001 0.0002 0.0006 0.0113 0.0026 0 0.0004 0 0.0041 0
Nucleolus 0.0001 0.0001 0 0 0.0004 0 0.0016 0.0001 0.0003 0 0.001 0.0001 0.003 0.0001 0.0001 0 0.0002 0.0001
Nucleus 0.0001 0 0 0 0.0008 0 0.0008 0 0.0001 0.0001 0.0004 0.0039 0.0032 0.0001 0.0001 0 0.0005 0
Peroxisomes 0.0274 0.0139 0.0145 0.0107 0.0029 0.0086 0.0504 0.0184 0.0132 0.0069 0.0046 0.0075 0.0298 0.0251 0.0437 0.0049 0.0037 0.0054
Punctate Nuclear 0 0 0 0 0.0061 0 0.0038 0 0.0001 0 0 0.0004 0.0043 0.0001 0.0036 0 0.0009 0
Vacuole 0.0006 0.001 0.0004 0.0002 0.0007 0.0002 0.0031 0.0007 0.0005 0.0053 0.0036 0.0004 0.0028 0.0008 0.0003 0.0004 0.0003 0.0005
Vacuole Periphery 0.0029 0.002 0.0008 0.0006 0.0021 0.0012 0.0041 0.0012 0.0013 0.0121 0.0046 0.001 0.0033 0.0009 0.0015 0.0015 0.0011 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.1685 11.0918 17.637 24.2055 14.3636 16.8298 13.6181 20.8156 15.6633 19.1301
Translational Efficiency 0.916 1.502 1.0888 0.8193 1.4315 0.9935 1.099 0.8672 1.4022 1.1534

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
150 473 1369 965 1807 1963 743 1178 1957 2436 2112 2143

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1127.02 1295.88 1303.18 1569.39 1110.14 1216.29 1605.84 1605.71 1111.43 1231.74 1409.66 1589.35
Standard Deviation 193.55 246.66 289.88 376.99 215.49 224.57 277.53 320.49 213.93 231.18 320.08 347.54
Intensity Change Log 2 — 0.201419 0.209523 0.477691 — 0.131746 0.532587 0.532470 — 0.167266 0.378865 0.505134

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.012081 0.013109 0.002727 0.010865 0.019491 0.007064 0.007622 0.013030 0.018923 0.008238 0.004449 0.012055
Bud Neck 0.215133* 0.234528* 0.295870* 0.220521* 0.238218* 0.279800* 0.279357* 0.200597* 0.236449* 0.271009* 0.290061* 0.209569*
Bud Site 0.586608* 0.592003* 0.544888* 0.627530* 0.519405* 0.574744* 0.541955* 0.662002* 0.524556* 0.578095* 0.543856* 0.646479*
Cell Periphery 0.004294 0.008757 0.010752 0.007617 0.017098 0.016770 0.005068 0.005881 0.016116 0.015214 0.008752 0.006663
Cytoplasm 0.028603 0.019792 0.025034 0.018804 0.020097 0.021035 0.018254 0.024853 0.020749 0.020793 0.022649 0.022129
Cytoplasmic Foci 0.059344 0.042460 0.043293 0.045617 0.062998 0.036967 0.077725 0.032579 0.062718 0.038034 0.055406 0.038450
Eisosomes 0.002551 0.005723 0.005236 0.005202 0.015797 0.012762 0.007868 0.004977 0.014781 0.011395 0.006162 0.005078
Endoplasmic Reticulum 0.002181 0.000526 0.000545 0.000288 0.000936 0.000326 0.000215 0.000260 0.001031 0.000365 0.000429 0.000273
Endosome 0.004266 0.002252 0.002801 0.002435 0.003493 0.001816 0.001470 0.002978 0.003553 0.001901 0.002333 0.002734
Golgi 0.001244 0.000999 0.000745 0.001473 0.001252 0.001111 0.000875 0.002619 0.001251 0.001089 0.000791 0.002103
Lipid Particles 0.000413 0.000212 0.000188 0.000251 0.000505 0.000481 0.000159 0.000484 0.000498 0.000429 0.000178 0.000379
Mitochondria 0.018391 0.028184 0.010836 0.015466 0.027999 0.013468 0.010783 0.012402 0.027262 0.016325 0.010817 0.013782
Mitotic Spindle 0.003960 0.003343 0.003879 0.002646 0.002944 0.001970 0.002379 0.003666 0.003022 0.002237 0.003351 0.003207
None 0.000563 0.000498 0.000631 0.000425 0.001524 0.000612 0.000322 0.000387 0.001450 0.000590 0.000522 0.000404
Nuclear Periphery 0.000069 0.000069 0.000126 0.000076 0.000074 0.000030 0.000087 0.000077 0.000074 0.000038 0.000113 0.000076
Nuclear Periphery Foci 0.000613 0.000951 0.000866 0.000551 0.001378 0.001022 0.001140 0.000536 0.001319 0.001008 0.000963 0.000543
Nucleolus 0.002448 0.006400 0.005486 0.006608 0.009522 0.001515 0.006697 0.004917 0.008980 0.002463 0.005912 0.005678
Nucleus 0.003886 0.003254 0.006525 0.002996 0.007462 0.002585 0.003191 0.002775 0.007188 0.002715 0.005352 0.002875
Peroxisomes 0.041295 0.024370 0.010864 0.013531 0.033313 0.016766 0.017084 0.009902 0.033925 0.018243 0.013052 0.011536
Vacuole 0.010721 0.011592 0.025104 0.014421 0.015051 0.008733 0.015819 0.013187 0.014719 0.009288 0.021838 0.013743
Vacuole Periphery 0.001337 0.000980 0.003603 0.002680 0.001443 0.000421 0.001931 0.001889 0.001435 0.000530 0.003015 0.002245

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.20 2.07 0.24 0.57 -3.91 5.55 4.55 2.30 -2.66 -2.07 4.97 7.33 2.93 -2.14 -4.83
Bud Neck -0.90 -3.81 -0.20 1.24 7.73 -5.15 -4.09 4.75 9.48 7.88 -4.65 -6.85 4.10 9.13 11.53
Bud Site -0.20 1.70 -1.55 -2.18 -7.30 -5.93 -1.79 -13.59 -8.52 -9.85 -6.11 -1.94 -13.63 -8.17 -12.56
Cell Periphery -2.59 -5.31 -2.42 0.51 1.95 0.21 8.28 8.70 8.41 -0.83 0.64 5.82 7.65 7.30 2.06
Cytoplasm 1.63 0.29 1.59 -0.07 2.56 -0.53 0.78 -2.55 -2.20 -3.35 -0.04 -1.88 -1.40 -1.50 0.53
Cytoplasmic Foci 1.45 1.39 1.18 -0.60 -0.42 6.31 -2.53 7.16 1.27 7.85 6.40 1.57 6.22 -0.16 5.04
Eisosomes -3.25 -3.10 -2.94 0.57 0.03 1.92 5.56 6.87 5.77 2.40 2.40 6.92 7.41 6.39 1.41
Endoplasmic Reticulum 0.89 0.85 1.03 0.98 2.13 5.71 6.86 7.43 0.89 -0.15 3.82 2.96 4.68 1.21 2.13
Endosome 1.18 0.83 0.99 -0.16 0.50 4.29 5.47 0.76 -1.63 -1.97 4.49 3.22 1.43 -1.44 -0.53
Golgi 0.81 2.44 -0.45 -0.90 -1.55 0.62 2.17 -1.35 -1.47 -1.65 0.82 4.36 -1.41 -1.63 -2.16
Lipid Particles 1.08 1.21 0.90 -0.36 -0.68 0.09 4.01 0.12 -0.02 -1.65 0.25 4.06 0.96 0.15 -1.72
Mitochondria -1.23 1.15 0.43 2.43 -1.96 5.37 5.79 5.52 0.44 -0.70 4.15 6.76 5.33 1.27 -1.84
Mitotic Spindle 0.28 -0.04 0.67 0.44 1.76 1.77 0.61 -0.88 -2.05 -1.28 1.41 -0.83 -0.38 -1.72 0.44
None 0.48 -0.51 0.97 1.13 2.33 4.19 5.70 5.42 2.88 -1.30 4.32 4.52 5.42 2.95 2.02
Nuclear Periphery -0.04 -2.11 -0.43 -0.30 2.16 5.70 -1.13 -0.33 -3.91 0.77 4.22 -2.50 -0.41 -4.34 2.23
Nuclear Periphery Foci -1.05 -1.76 0.48 1.28 2.76 1.50 1.06 5.51 2.13 2.92 1.47 2.41 6.05 2.49 3.96
Nucleolus -1.81 -2.83 -2.74 -0.03 -0.72 7.29 1.40 2.83 -2.58 1.10 5.96 2.45 2.52 -3.11 0.36
Nucleus 0.36 -1.71 0.46 0.18 4.37 4.43 3.36 4.58 -0.40 1.22 4.46 1.08 4.56 -0.48 4.64
Peroxisomes 1.71 3.23 2.93 3.48 -1.68 6.63 6.88 10.29 4.09 4.89 6.54 9.57 10.26 4.57 1.36
Vacuole -0.46 -5.19 -1.62 -1.64 5.20 6.72 -0.97 0.93 -4.38 1.46 5.91 -5.25 0.33 -5.47 5.47
Vacuole Periphery 0.70 -2.68 -1.55 -2.21 0.90 3.56 -1.16 -0.87 -3.22 0.26 3.36 -2.93 -1.67 -4.10 1.26
Standard name
Human Ortholog
Description Mitochondrial NADH kinase; phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Pos5

Pos5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pos5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available