ORF
Human Ortholog
Description K48-specific deubiquitinating (DUB) enzyme; MINDY family endo-type deubiquitinase that preferentially cleaves long K48-linked polyubiquitin chains between moieties; diploid deletion strain exhibits high budding index; GFP-fusion protein localizes to the cytoplasm endoplasmic reticulum and cell periphery in high-throughput studies; YPL191C has a paralog, YGL082W, that arose from the whole genome duplication; ortholog of human MINDY2/FAM63B
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.92 0.88 0.77 0.66 0.65 0.56 0.63 0.45 0.6 1.0 0.99 0.99 0.92 0.91 0.96 0.95 0.9 0.91 0.98 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.22 0.31 0.32 0.42 0.31 0.58 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.06 0.07 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.13 0.14 0.14 0.18 0.17 0.12 0.15 0.13 0.18 0 0 0 0.08 0.13 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 2 0
Bud 0 0 2 1 0 0 6 9 4 5 8 3 0 0 0 0 0 0 0 1 0 0 0 4
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 4 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 1 2 4 3 1 2 0 0 1 0 0 1 5 7 9 0 0 0 0 0 0
Cytoplasm 231 297 336 223 306 322 324 300 190 277 151 205 185 274 308 252 217 325 228 281 330 228 316 391
Endoplasmic Reticulum 0 3 1 0 0 0 0 0 1 0 0 0 0 1 0 15 11 10 0 1 4 1 1 3
Endosome 0 0 1 2 1 4 1 1 1 0 0 1 0 0 0 0 0 1 2 4 2 0 0 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 2 0 0 1 0
Mitochondria 4 2 2 7 17 90 153 146 142 137 193 111 0 0 0 1 0 0 2 1 7 0 5 4
Nucleus 0 2 1 1 0 15 20 27 24 26 17 19 0 1 3 2 2 3 0 4 1 0 2 3
Nuclear Periphery 0 1 0 0 0 0 0 0 0 3 0 1 0 0 0 0 0 0 1 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 1 0 2 0 1 2 0 0 1 1 0 0 0 0 0 0 0 3 2 0 1 3
Vac/Vac Membrane 2 6 11 31 47 57 86 80 40 65 44 63 4 4 3 23 31 16 0 2 3 1 2 3
Unique Cell Count 236 307 348 243 348 417 488 462 340 440 333 342 185 277 310 274 238 338 241 312 361 234 340 423
Labelled Cell Count 238 312 357 266 375 492 595 566 405 517 415 405 189 280 315 299 269 366 241 312 361 234 340 423


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.8 6.4 6.0 6.1 5.0 4.8 5.1 4.5 4.7 4.2 4.8 7.0 7.4 7.6 7.8 8.6 8.1 6.7 7.3 7.7
Std Deviation (1e-4) 0.6 0.9 1.0 1.5 1.7 1.2 1.0 1.1 0.9 1.1 0.9 1.0 1.4 0.9 1.1 1.1 1.4 1.2 1.3 1.5 1.6
Intensity Change (Log2) – – – -0.1 -0.08 -0.35 -0.41 -0.32 -0.52 -0.44 -0.6 -0.43 0.13 0.2 0.24 0.29 0.42 0.33 0.06 0.18 0.27

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.5 -4.3 -7.7 -10.5 -10.8 -12.6 -11.3 -14.8 -11.7 2.6 1.9 2.5 -2.5 -2.8 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.1 0 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.5 8.9 0 11.3 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 3.2 3.5 4.3 4.7 4.3 3.9 4.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 4.5 4.9 5.1 6.4 6.3 4.3 5.5 4.8 6.5 -0.7 -1.4 -1.9 2.9 4.5 1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9093 2.9694 2.6845 2.436 2.688 2.5191 1.4975 2.0322 2.0639 1.5682 1.4625 1.9522 1.1646 1.7564 1.3858 1.4153 0.8461 1.4239
Actin 0.0329 0.0015 0.0101 0.0218 0.0064 0.0027 0.0003 0.0005 0.0005 0.0002 0.0008 0.0005 0.0172 0.0009 0.0026 0.0006 0.0001 0.0116
Bud 0.0022 0.0032 0.0034 0.0014 0.0005 0.0004 0.0001 0.0003 0.0009 0.0001 0.0006 0.0002 0.0003 0.0009 0.0011 0.0002 0.0001 0.0003
Bud Neck 0.0097 0.0007 0.0045 0.0044 0.0053 0.0025 0.0004 0.0011 0.0009 0.0012 0.0012 0.0026 0.0062 0.0008 0.0006 0.0006 0.0004 0.0054
Bud Periphery 0.0036 0.0026 0.0055 0.0012 0.0006 0.0003 0.0001 0.0001 0.0004 0.0001 0.0009 0.0001 0.0003 0.0004 0.0011 0.0001 0 0.0002
Bud Site 0.0094 0.0086 0.0041 0.0069 0.0014 0.0006 0.0005 0.0055 0.004 0.0003 0.0006 0.0002 0.001 0.0082 0.001 0.0005 0.0001 0.0005
Cell Periphery 0.0015 0.0002 0.0003 0.0001 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.3863 0.6506 0.537 0.5639 0.3433 0.5671 0.6078 0.738 0.6755 0.6623 0.7401 0.7055 0.6423 0.748 0.7062 0.7035 0.7349 0.7379
Cytoplasmic Foci 0.031 0.013 0.0122 0.0279 0.0732 0.0257 0.0081 0.0073 0.0071 0.0065 0.0094 0.0105 0.0167 0.0092 0.0122 0.0197 0.0065 0.0125
Eisosomes 0.0009 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0 0 0.0001 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0057 0.0036 0.0055 0.0035 0.0031 0.0034 0.0017 0.002 0.0026 0.001 0.0022 0.0018 0.0035 0.0027 0.0037 0.0043 0.0022 0.003
Endosome 0.0267 0.0071 0.0103 0.026 0.0397 0.0125 0.003 0.0036 0.0023 0.0013 0.0152 0.0044 0.0064 0.0037 0.004 0.0101 0.0044 0.0066
Golgi 0.0095 0.0007 0.0018 0.0151 0.0053 0.0021 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0012 0.0003 0.0004 0.0004 0.0001 0.0011
Lipid Particles 0.0158 0.0004 0.001 0.003 0.0392 0.005 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0054 0.0001 0.0002 0.0004 0 0.0007
Mitochondria 0.007 0.0017 0.0016 0.0035 0.0106 0.0055 0.0004 0.0003 0.0004 0.0004 0.0005 0.0003 0.0006 0.0003 0.0005 0.0004 0.0002 0.0005
None 0.3808 0.253 0.3636 0.2355 0.3785 0.3175 0.343 0.1912 0.2631 0.2986 0.1743 0.2301 0.2735 0.1882 0.2396 0.2305 0.2376 0.1852
Nuclear Periphery 0.0084 0.0047 0.0057 0.0058 0.004 0.0051 0.0019 0.0017 0.0027 0.0008 0.0021 0.0019 0.0025 0.003 0.002 0.003 0.0014 0.0019
Nucleolus 0.0037 0.0008 0.0006 0.0007 0.0012 0.0006 0.0006 0.0005 0.0005 0.0003 0.0003 0.0004 0.0004 0.0002 0.0001 0.0002 0 0.0002
Nucleus 0.0175 0.0317 0.019 0.0504 0.0048 0.022 0.0224 0.0334 0.0304 0.0227 0.0439 0.0301 0.0097 0.0278 0.0201 0.0174 0.0095 0.0269
Peroxisomes 0.0221 0.0025 0.0009 0.0069 0.0516 0.0097 0.0002 0.0002 0.0002 0.0001 0.0002 0.0003 0.006 0.0002 0.0007 0.0003 0.0001 0.0006
Punctate Nuclear 0.0206 0.0109 0.0095 0.0188 0.0273 0.0146 0.008 0.0123 0.0067 0.0035 0.0061 0.01 0.0049 0.0035 0.0025 0.0053 0.0011 0.0033
Vacuole 0.0038 0.0023 0.0026 0.0025 0.0026 0.002 0.001 0.0015 0.0011 0.0005 0.0011 0.0008 0.0018 0.0014 0.0012 0.0022 0.0011 0.0013
Vacuole Periphery 0.0009 0.0004 0.0005 0.0006 0.0011 0.0005 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0003 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.0942 1.8216 3.3792 5.9955 6.6049 6.1097 5.5253 6.5262 9.2465 7.5833
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1046 998 2664 1069 2177 2072 31 569 3223 3070 2695 1638

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 643.98 759.91 853.18 868.05 761.01 790.85 1209.55 896.02 723.03 780.79 857.28 877.77
Standard Deviation 102.31 138.78 113.39 128.23 96.92 105.68 215.67 134.93 112.89 118.36 121.20 131.28
Intensity Change Log 2 — 0.238813 0.405834 0.430762 — 0.055489 0.668483 0.235616 — 0.142416 0.553995 0.328348

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000140 0.000287 0.001071 0.001088 0.000087 0.000173 0.001780 0.000621 0.000104 0.000210 0.001079 0.000925
Bud Neck 0.006500 0.005603 0.011960 0.007562 0.003465 0.009811 0.005838 0.004241 0.004450 0.008443 0.011890 0.006409
Bud Site 0.002972 0.008451 0.037260 0.024652 0.001881 0.010123 0.073874 0.040748 0.002235 0.009580 0.037681 0.030244
Cell Periphery 0.000126 0.000131 0.000165 0.000220 0.000068 0.000067 0.000447 0.000137 0.000087 0.000088 0.000169 0.000191
Cytoplasm 0.567092 0.643129 0.546776 0.467147 0.623323 0.599809 0.289554 0.566297 0.605074 0.613892 0.543817 0.501589
Cytoplasmic Foci 0.122631 0.087965 0.007186 0.013126 0.063523 0.060623 0.014261 0.012779 0.082706 0.069512 0.007267 0.013006
Eisosomes 0.000072 0.000038 0.000024 0.000045 0.000033 0.000028 0.000073 0.000034 0.000046 0.000031 0.000024 0.000041
Endoplasmic Reticulum 0.001412 0.001458 0.001364 0.005775 0.000806 0.000824 0.007993 0.001975 0.001002 0.001030 0.001440 0.004455
Endosome 0.008666 0.007470 0.002076 0.006154 0.002975 0.003621 0.009936 0.004314 0.004822 0.004872 0.002166 0.005515
Golgi 0.000583 0.001565 0.000558 0.006094 0.000218 0.000812 0.000135 0.000266 0.000337 0.001057 0.000553 0.004070
Lipid Particles 0.005017 0.002462 0.000688 0.001473 0.001560 0.001249 0.003073 0.000989 0.002682 0.001644 0.000715 0.001305
Mitochondria 0.004302 0.001838 0.001867 0.003094 0.000563 0.003677 0.000412 0.001264 0.001776 0.003079 0.001850 0.002458
Mitotic Spindle 0.000648 0.003650 0.024262 0.018244 0.002263 0.013408 0.000980 0.039476 0.001739 0.010236 0.023994 0.025619
None 0.010826 0.011889 0.005842 0.006667 0.004391 0.003558 0.000755 0.015255 0.006479 0.006267 0.005783 0.009651
Nuclear Periphery 0.000739 0.000511 0.001493 0.001671 0.000626 0.000768 0.001319 0.003415 0.000663 0.000685 0.001491 0.002277
Nuclear Periphery Foci 0.001432 0.001811 0.000628 0.002674 0.000622 0.000393 0.007879 0.001685 0.000885 0.000854 0.000711 0.002331
Nucleolus 0.001982 0.001601 0.000421 0.000348 0.000534 0.000901 0.000247 0.001013 0.001004 0.001129 0.000419 0.000579
Nucleus 0.207843 0.161242 0.309507 0.305880 0.263702 0.238316 0.151737 0.179919 0.245574 0.213261 0.307692 0.262124
Peroxisomes 0.002627 0.002933 0.000928 0.000592 0.000900 0.002245 0.000517 0.000354 0.001460 0.002469 0.000923 0.000509
Vacuole 0.053547 0.055052 0.045467 0.126573 0.028194 0.048779 0.428690 0.119877 0.036422 0.050818 0.049875 0.124247
Vacuole Periphery 0.000844 0.000914 0.000457 0.000920 0.000265 0.000812 0.000499 0.005340 0.000453 0.000845 0.000457 0.002456

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.49 -2.71 -4.38 -3.48 -0.50 -2.02 -4.36 -5.86 -4.96 3.15 -3.37 -2.93 -5.53 -4.68 0.07
Bud Neck 0.70 -2.94 0.04 -0.78 3.41 -6.03 -1.44 -1.71 5.29 0.73 -5.03 -6.42 -1.86 3.24 4.72
Bud Site -4.33 -10.86 -5.97 -2.67 3.91 -6.57 -1.86 -4.45 -1.86 1.36 -7.82 -11.52 -7.67 -3.01 4.04
Cell Periphery -0.17 -0.99 -4.88 -3.33 -3.42 0.40 -4.00 -5.74 -5.29 3.25 0.09 -5.30 -9.63 -8.20 -1.70
Cytoplasm -6.11 9.06 18.05 24.42 12.30 3.91 8.95 19.61 16.78 -3.18 -0.48 19.36 30.73 30.19 12.77
Cytoplasmic Foci 5.52 24.67 24.10 18.65 -3.46 0.82 17.92 22.83 20.96 -0.65 4.33 34.37 33.07 28.53 -3.87
Eisosomes 7.93 11.44 3.06 -6.51 -11.83 2.32 -4.12 -7.74 -9.29 1.59 7.31 8.03 -5.66 -12.97 -13.35
Endoplasmic Reticulum -0.54 -2.99 -12.44 -13.06 -11.96 -0.79 -4.18 -10.12 -9.98 2.60 -0.93 -9.13 -16.79 -16.85 -12.13
Endosome 1.09 7.32 5.05 4.54 -2.81 -1.72 -1.81 1.16 2.55 2.13 -0.11 7.86 3.65 3.93 -3.08
Golgi -3.07 1.55 -1.44 -0.42 -1.68 -3.34 1.83 1.61 3.71 -0.32 -4.53 -0.07 -1.61 -0.47 -1.56
Lipid Particles 4.99 9.03 6.57 2.42 -8.05 1.96 -2.30 1.24 -0.90 2.65 5.20 9.93 5.01 -0.46 -8.43
Mitochondria 1.62 1.94 1.87 0.20 0.00 -4.18 0.35 -1.72 3.77 -1.63 -1.77 0.34 0.79 2.67 0.66
Mitotic Spindle -2.71 -10.01 -6.10 -4.30 1.65 -4.97 -0.01 -5.32 -2.85 -5.28 -5.48 -9.11 -7.64 -3.50 0.08
None -0.27 3.74 3.49 3.19 0.38 1.26 4.79 -4.23 -5.18 -7.09 0.49 1.39 -0.65 -1.10 -2.09
Nuclear Periphery 1.42 -11.37 -12.59 -19.57 -3.62 -1.94 -2.67 -4.11 -3.79 -1.35 -0.83 -17.81 -10.31 -10.34 -2.30
Nuclear Periphery Foci -1.93 4.20 -6.12 -3.85 -10.72 2.94 -2.71 -6.78 -8.08 2.21 -0.24 0.70 -10.85 -10.54 -11.07
Nucleolus 1.11 4.68 4.88 6.27 1.32 -2.51 3.79 -0.69 1.21 -2.63 -0.82 4.55 3.88 5.15 -0.47
Nucleus 5.32 -19.69 -12.72 -18.18 4.42 3.67 1.33 0.68 -1.65 -1.14 5.87 -19.29 -8.69 -13.13 8.18
Peroxisomes -0.43 3.65 4.48 4.44 1.43 -2.91 1.88 2.46 6.42 1.27 -2.48 2.55 4.49 7.48 1.75
Vacuole -1.14 -4.78 -21.80 -20.98 -19.75 -10.42 -7.78 -21.03 -18.61 3.06 -8.61 -13.73 -32.30 -28.82 -24.10
Vacuole Periphery -0.26 3.05 2.56 3.43 -0.66 -3.07 -0.78 -1.24 -0.59 -1.16 -2.82 1.50 -0.84 0.39 -1.29
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
ORF
Human Ortholog
Description K48-specific deubiquitinating (DUB) enzyme; MINDY family endo-type deubiquitinase that preferentially cleaves long K48-linked polyubiquitin chains between moieties; diploid deletion strain exhibits high budding index; GFP-fusion protein localizes to the cytoplasm endoplasmic reticulum and cell periphery in high-throughput studies; YPL191C has a paralog, YGL082W, that arose from the whole genome duplication; ortholog of human MINDY2/FAM63B
Localization
Cell Percentages cytoplasm (81%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YPL191C

YPL191C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl191c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available