Standard name
Human Ortholog
Description Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.09 0.11 0.1 0.08 0.12 0.1 0.08 0.07 0.08 0.1 0.15 0.19 0.12 0.12 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.47 0.29 0.1 0.21 0.29 0.63 0.69 0.59 0.59 0.73 0.8 0.75 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Nucleus 0.8 0.81 0.85 0.77 0.69 0.56 0.54 0.52 0.54 0.53 0.56 0.46 0.89 0.83 0.74 0.79 0.74 0.74 0.79 0.76 0.78 0.79 0.71 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0.05 0 0.11 0.12 0.08 0.07 0.08 0.06 0.07 0.1 0 0 0.05 0.07 0.13 0.15 0.13 0.11 0.12 0.1 0.14 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2 0 2 3 5 15 10 11 6 3 7 0 0 0 0 0 0 1 0 4 4 7 3
Bud Neck 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 3 5 15 12 6 9 23 13 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 2 2 16 28 43 41 57 67 49 43 34 45 42 85 118 21 24 19 1 1 5 6 24 12
Endoplasmic Reticulum 0 0 1 0 0 0 0 2 0 0 1 0 0 1 1 5 10 3 0 0 0 0 0 1
Endosome 0 0 3 2 3 1 1 8 4 1 1 1 1 7 22 4 4 2 1 4 1 1 3 3
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 1 0 1 0
Mitochondria 45 24 30 63 117 265 480 330 292 407 390 450 11 2 8 11 4 8 0 2 4 2 12 7
Nucleus 76 68 252 236 274 236 376 292 268 293 276 274 376 475 452 140 144 100 78 65 260 178 393 296
Nuclear Periphery 2 0 0 1 1 4 2 0 3 4 3 3 0 1 1 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 2 2 1 1 7 1 6 7 3 1 0 0 0 2 1 0 0 1 0 5 14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 1 2 4 3 2 1 2 6 3 0 0 1 0 2 1 5 5 13 12
Vac/Vac Membrane 6 6 15 13 43 52 58 38 38 34 35 61 3 8 29 12 26 21 13 9 40 22 79 67
Unique Cell Count 95 84 295 305 398 422 697 562 498 558 490 599 423 573 613 177 194 136 100 86 332 226 552 428
Labelled Cell Count 131 102 318 351 490 611 1009 770 676 805 774 859 441 582 632 194 216 155 100 86 332 226 552 428


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.6 4.7 5.9 4.6 3.5 3.4 3.7 3.8 3.4 3.1 3.1 5.9 5.8 5.6 7.0 7.4 7.0 5.8 6.1 6.4
Std Deviation (1e-4) 0.6 0.9 1.2 5.3 2.3 1.0 0.9 2.0 2.6 2.2 0.9 0.9 1.1 1.2 1.2 1.3 1.8 1.5 1.2 1.6 1.9
Intensity Change (Log2) – – – 0.35 -0.02 -0.41 -0.45 -0.32 -0.29 -0.47 -0.58 -0.57 0.34 0.31 0.27 0.59 0.66 0.6 0.31 0.38 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0.8 3.2 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1016 1.4588 1.324 0.9348 1.61 1.3606 -0.1913 0.1208 0.0082 -0.3676 -0.6796 -0.2696 -0.5829 -0.4292 -0.7052 -1.0143 -0.7792 -0.7209
Actin 0.0241 0 0.0012 0.0002 0.002 0.0012 0.0165 0 0.0204 0.0317 0.0022 0.0004 0.0458 0 0.0083 0 0.0805 0.0047
Bud 0.0007 0 0.0001 0.0001 0.0003 0.0001 0.0011 0 0.0004 0.0003 0.0001 0 0.0005 0 0.0001 0 0.0007 0.0001
Bud Neck 0.0027 0.0003 0.0009 0.0001 0.0006 0.0015 0.0094 0.0001 0.0045 0.0018 0.0003 0.0014 0.0017 0.0008 0.0001 0.0001 0.0008 0.0012
Bud Periphery 0.0018 0 0.0001 0.0001 0.0004 0.0004 0.0021 0 0.0008 0.0003 0.0001 0.0001 0.0008 0 0.0002 0 0.0013 0.0003
Bud Site 0.0018 0.0001 0.003 0.0001 0.001 0.0001 0.0101 0.0001 0.0041 0.0008 0.0002 0.0001 0.0012 0.0003 0.0004 0 0.0053 0.0004
Cell Periphery 0.0002 0 0.0001 0 0.0001 0.0001 0.0007 0 0.0003 0.0002 0 0 0.0002 0 0 0 0.0002 0
Cytoplasm 0.0259 0.0056 0.0116 0.0028 0.0019 0.0019 0.0086 0.0061 0.0119 0.0221 0.0005 0.0016 0.0187 0.0055 0.0018 0.0107 0.0027 0.0026
Cytoplasmic Foci 0.013 0.0001 0.0012 0.0033 0.0029 0.002 0.0083 0.0001 0.0149 0.0088 0.0054 0.0004 0.0149 0.0001 0.0035 0 0.0336 0.0036
Eisosomes 0.0003 0 0 0 0.0001 0 0.0004 0 0.0004 0.0004 0 0 0.0004 0 0.0001 0 0.0009 0.0001
Endoplasmic Reticulum 0.0067 0.0003 0.0006 0.0001 0.003 0.0004 0.0041 0.0005 0.0024 0.0025 0.0002 0.0003 0.0049 0.0003 0.0001 0.0014 0.0016 0.0005
Endosome 0.0422 0.0005 0.0018 0.0081 0.033 0.0032 0.0258 0.0003 0.0156 0.0049 0.0073 0.0023 0.0253 0.0003 0.0011 0.0003 0.0182 0.0066
Golgi 0.0085 0 0.0008 0.0057 0.0052 0.0016 0.005 0 0.0063 0.0019 0.0045 0.0003 0.006 0 0.001 0 0.0131 0.0042
Lipid Particles 0.013 0.0001 0.0008 0.0378 0.0041 0.0048 0.0136 0 0.0089 0.0108 0.0304 0.0009 0.0054 0 0.0016 0 0.0231 0.0088
Mitochondria 0.0078 0.0002 0.0004 0.0388 0.0734 0.0034 0.0129 0.0001 0.0151 0.0081 0.0012 0.0003 0.0083 0.0002 0.0018 0.0003 0.0413 0.0038
None 0.0285 0.0072 0.0122 0.0002 0.0004 0.0031 0.0053 0.0066 0.0051 0.0021 0.0003 0.0001 0.013 0.0004 0.0076 0.0027 0.0035 0.0007
Nuclear Periphery 0.0731 0.0131 0.0112 0.0021 0.0082 0.0055 0.0278 0.0101 0.0178 0.0139 0.0038 0.0081 0.0238 0.0066 0.0071 0.018 0.0092 0.0076
Nucleolus 0.0115 0.0069 0.0078 0.0019 0.0058 0.0116 0.0242 0.004 0.0032 0.005 0.0059 0.0156 0.0039 0.0057 0.0038 0.0017 0.0079 0.0117
Nucleus 0.6837 0.9516 0.9341 0.8766 0.8254 0.9238 0.7978 0.9693 0.8458 0.8736 0.9285 0.9556 0.8013 0.9756 0.9463 0.9636 0.6964 0.9086
Peroxisomes 0.0055 0 0.0004 0.0156 0.0008 0.0061 0.0083 0 0.0052 0.0023 0.0039 0.0002 0.0028 0 0.0085 0 0.0222 0.0167
Punctate Nuclear 0.0384 0.013 0.0106 0.0054 0.0275 0.0277 0.011 0.0021 0.0143 0.0045 0.0046 0.0119 0.017 0.0036 0.0061 0.0006 0.0351 0.016
Vacuole 0.0062 0.0007 0.0008 0.0004 0.0026 0.0007 0.0052 0.0004 0.0014 0.0025 0.0002 0.0003 0.0026 0.0005 0.0002 0.0003 0.0008 0.001
Vacuole Periphery 0.0045 0.0002 0.0002 0.0006 0.0015 0.0007 0.0019 0.0001 0.0011 0.0016 0.0003 0.0002 0.0017 0.0001 0.0002 0.0003 0.0016 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.331 12.9107 13.4124 14.3161 15.6044 10.5853 13.2715 14.3906 11.6509 14.896
Translational Efficiency 1.0165 1.2853 0.9459 0.7155 0.8158 1.465 0.9132 0.7283 0.7061 0.9067

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1648 136 1331 655 1210 513 116 955 2858 649 1447 1610

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.54 813.06 888.83 873.37 699.86 775.60 975.30 892.66 701.41 783.45 895.76 884.81
Standard Deviation 86.74 95.83 106.96 116.80 83.75 110.31 122.23 122.34 85.50 108.52 110.78 120.49
Intensity Change Log 2 — 0.210781 0.339327 0.314013 — 0.148246 0.478780 0.351044 — 0.179912 0.410605 0.332612

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000058 0.000941 0.000552 0.000899 0.000089 0.000170 0.000570 0.000825 0.000071 0.000332 0.000553 0.000855
Bud Neck 0.001420 0.011419 0.010450 0.012324 0.001268 0.003561 0.002623 0.011802 0.001355 0.005208 0.009822 0.012015
Bud Site 0.000540 0.008290 0.002988 0.005355 0.001041 0.002976 0.000633 0.007669 0.000752 0.004089 0.002799 0.006727
Cell Periphery 0.000102 0.000257 0.000096 0.000132 0.000154 0.000182 0.000083 0.000134 0.000124 0.000197 0.000095 0.000133
Cytoplasm 0.061011 0.046707 0.012732 0.014578 0.099742 0.070533 0.003722 0.033825 0.077408 0.065540 0.012010 0.025995
Cytoplasmic Foci 0.008021 0.009047 0.000336 0.000985 0.014495 0.014053 0.000258 0.002054 0.010762 0.013004 0.000329 0.001619
Eisosomes 0.000018 0.000059 0.000025 0.000026 0.000029 0.000040 0.000041 0.000030 0.000023 0.000044 0.000026 0.000028
Endoplasmic Reticulum 0.001527 0.009669 0.006481 0.012530 0.001435 0.002986 0.009414 0.007402 0.001488 0.004386 0.006716 0.009488
Endosome 0.000511 0.001423 0.000521 0.002123 0.001284 0.002508 0.000444 0.005512 0.000839 0.002281 0.000515 0.004133
Golgi 0.000997 0.000336 0.000052 0.003019 0.000430 0.000384 0.000042 0.003242 0.000757 0.000374 0.000051 0.003151
Lipid Particles 0.001697 0.002036 0.000260 0.000283 0.002679 0.004452 0.000523 0.001773 0.002113 0.003946 0.000281 0.001167
Mitochondria 0.001925 0.003244 0.001515 0.004877 0.001376 0.004444 0.000506 0.007039 0.001693 0.004193 0.001434 0.006159
Mitotic Spindle 0.000957 0.004205 0.001635 0.009709 0.001537 0.002853 0.000093 0.016189 0.001202 0.003136 0.001511 0.013553
None 0.006514 0.005006 0.004167 0.005939 0.011042 0.010116 0.001887 0.009576 0.008431 0.009045 0.003985 0.008096
Nuclear Periphery 0.001056 0.001296 0.000994 0.003234 0.000845 0.004322 0.000766 0.001824 0.000967 0.003688 0.000976 0.002398
Nuclear Periphery Foci 0.000231 0.000994 0.000186 0.001768 0.000388 0.001004 0.000324 0.000774 0.000297 0.001002 0.000197 0.001179
Nucleolus 0.002417 0.003676 0.002411 0.001539 0.003120 0.004252 0.001567 0.002123 0.002715 0.004131 0.002343 0.001885
Nucleus 0.908052* 0.875367* 0.949406* 0.907384* 0.854027* 0.855982* 0.969092* 0.868957* 0.885179* 0.860044* 0.950984* 0.884590*
Peroxisomes 0.000949 0.003948 0.000148 0.000784 0.001208 0.001685 0.000135 0.000549 0.001058 0.002159 0.000147 0.000645
Vacuole 0.001801 0.011734 0.004847 0.008802 0.003584 0.011380 0.007197 0.012878 0.002556 0.011455 0.005035 0.011220
Vacuole Periphery 0.000199 0.000345 0.000198 0.003711 0.000227 0.002118 0.000079 0.005822 0.000211 0.001746 0.000188 0.004963

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.69 -15.21 -10.38 -0.43 -4.16 -2.17 -6.92 -10.28 -7.94 -0.09 -4.42 -16.02 -14.72 -7.05 -4.46
Bud Neck -2.78 -9.53 -10.23 -1.06 -2.05 -3.11 -4.56 -11.28 -7.55 -7.44 -4.08 -9.82 -15.21 -7.10 -2.55
Bud Site -2.49 -4.86 -3.91 1.13 -1.22 -0.95 -0.57 -4.42 -1.49 -4.38 -2.05 -4.46 -6.06 -0.81 -2.53
Cell Periphery -2.05 0.37 -1.37 1.74 -1.82 -0.45 2.41 2.25 1.07 -1.00 -1.59 2.62 0.10 1.62 -2.63
Cytoplasm 1.34 14.11 12.18 3.16 -1.33 3.73 18.58 12.47 6.05 -7.94 1.98 21.43 16.06 7.45 -5.50
Cytoplasmic Foci -0.43 8.24 6.94 2.73 -1.24 0.16 10.54 9.79 5.38 -2.99 -1.04 13.20 11.89 6.00 -2.73
Eisosomes -3.91 -6.88 -6.44 2.47 -2.12 -1.91 -3.68 -4.44 -0.31 1.90 -4.65 -5.09 -8.85 1.29 -3.90
Endoplasmic Reticulum -5.02 -18.91 -14.75 -1.68 -6.74 -4.73 -7.34 -18.77 -12.00 1.61 -6.68 -20.65 -23.25 -10.69 -6.56
Endosome -2.54 -1.05 -2.08 0.09 -1.88 -1.57 1.35 -1.61 -0.31 -2.52 -2.63 1.09 -2.49 0.03 -3.05
Golgi 1.14 1.78 -0.42 -0.93 -1.16 0.23 1.94 -1.26 -1.34 -1.81 1.11 2.23 -0.95 -1.56 -2.05
Lipid Particles -0.83 4.00 3.80 3.59 -1.25 -1.45 4.19 2.24 2.70 -0.79 -2.01 6.38 3.07 3.36 -1.50
Mitochondria -0.68 0.37 -1.48 -0.16 -1.96 -2.40 1.25 -2.28 0.10 -2.99 -2.31 0.17 -2.58 0.17 -2.89
Mitotic Spindle -1.34 -1.54 -3.15 -1.69 -2.71 -1.18 1.06 -4.75 -3.93 -5.28 -1.90 -1.25 -5.78 -4.24 -5.27
None 0.89 3.05 1.74 0.05 -1.12 0.40 5.89 2.30 1.33 -3.90 -0.27 5.03 2.38 1.57 -2.45
Nuclear Periphery -1.29 -1.80 -4.85 -3.77 -4.47 -1.89 -1.98 -5.12 0.50 -4.39 -1.89 -3.14 -6.85 0.21 -6.05
Nuclear Periphery Foci -2.10 0.00 -1.60 -0.23 -1.60 -1.54 -0.37 -2.67 -0.13 -2.03 -2.38 1.21 -2.80 -0.29 -3.05
Nucleolus -1.34 -0.42 1.24 1.76 1.61 -1.42 2.62 1.74 3.38 -1.70 -2.52 0.63 2.05 3.90 1.32
Nucleus 2.05 -6.47 0.78 -1.66 5.47 -0.02 -9.66 -1.51 -1.14 7.92 2.83 -12.00 0.50 -2.38 10.45
Peroxisomes -1.86 2.58 0.47 1.97 -1.27 -0.90 3.11 2.58 3.75 -1.84 -2.15 3.90 1.98 3.43 -1.66
Vacuole -3.04 -6.29 -7.15 0.01 -3.84 -3.99 -3.02 -8.63 -4.49 -1.91 -5.35 -6.26 -10.97 -3.74 -7.26
Vacuole Periphery -1.24 -0.10 -1.80 -1.64 -1.80 -2.05 2.12 -1.99 -0.32 -2.09 -2.11 0.25 -2.63 -0.56 -2.65
Standard name
Human Ortholog
Description Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
Localization
Cell Percentages nucleus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rsa1

Rsa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available