Standard name | |
---|---|
Human Ortholog | |
Description | Flavin adenine dinucleotide transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC1 has a paralog, FLC3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 40 | 59 | 112 | 49 | 29 | 24 | 16 | 19 | – | 10 | 16 | 24 | 61 | 67 | 41 | 124 | 60 | 56 | 17 | 29 | 47 | 21 | 17 | 21 |
Bud | 19 | 33 | 52 | 23 | 4 | 7 | 9 | 21 | – | 15 | 14 | 26 | 34 | 34 | 27 | 48 | 23 | 13 | 35 | 40 | 47 | 15 | 6 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 10 | 24 | 30 | 18 | 19 | 11 |
Bud Site | 0 | 15 | 18 | 4 | 4 | 4 | 4 | 1 | – | 1 | 3 | 4 | 14 | 13 | 7 | 14 | 7 | 2 | – | – | – | – | – | – |
Cell Periphery | 41 | 28 | 65 | 30 | 14 | 7 | 6 | 9 | – | 11 | 25 | 28 | 70 | 70 | 51 | 67 | 16 | 50 | 18 | 10 | 52 | 25 | 58 | 43 |
Cytoplasm | 31 | 34 | 77 | 111 | 107 | 203 | 215 | 292 | – | 236 | 295 | 353 | 48 | 48 | 49 | 202 | 113 | 94 | 3 | 5 | 3 | 3 | 8 | 4 |
Endoplasmic Reticulum | 48 | 39 | 44 | 70 | 57 | 28 | 33 | 33 | – | 25 | 17 | 23 | 10 | 12 | 12 | 16 | 13 | 13 | 41 | 30 | 57 | 24 | 46 | 64 |
Endosome | 1 | 1 | 9 | 5 | 15 | 10 | 12 | 16 | – | 5 | 3 | 12 | 0 | 0 | 0 | 6 | 4 | 2 | 6 | 3 | 3 | 1 | 2 | 0 |
Golgi | 6 | 0 | 1 | 0 | 6 | 0 | 0 | 4 | – | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 0 | 3 | 0 | 0 | 0 |
Mitochondria | 4 | 5 | 13 | 2 | 2 | 11 | 21 | 16 | – | 30 | 27 | 25 | 1 | 1 | 1 | 34 | 17 | 15 | 1 | 1 | 10 | 5 | 3 | 6 |
Nucleus | 4 | 1 | 4 | 4 | 26 | 22 | 60 | 41 | – | 35 | 38 | 40 | 4 | 4 | 3 | 68 | 45 | 27 | 0 | 0 | 0 | 2 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 3 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 6 | 2 | 4 | 3 | 2 | 11 | – | 2 | 2 | 10 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 4 | 2 | 2 | 0 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 7 | 0 | 1 | 1 | 1 | 3 | – | 0 | 1 | 3 | 11 | 11 | 6 | 9 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 58 | 26 | 71 | 31 | 217 | 183 | 243 | 343 | – | 226 | 212 | 280 | 5 | 4 | 3 | 184 | 129 | 108 | 111 | 99 | 166 | 50 | 58 | 37 |
Unique Cell Count | 190 | 177 | 347 | 257 | 398 | 426 | 507 | 704 | 512 | 533 | 688 | 174 | 174 | 136 | 594 | 343 | 317 | 257 | 250 | 432 | 171 | 227 | 201 | |
Labelled Cell Count | 254 | 241 | 484 | 333 | 488 | 503 | 624 | 809 | 596 | 656 | 829 | 258 | 264 | 201 | 774 | 433 | 388 | 257 | 250 | 432 | 171 | 227 | 201 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.9 | 11.1 | 10.7 | 10.3 | 10.5 | 9.3 | 9.4 | 9.6 | – | 9.1 | 9.0 | 9.7 | 11.6 | 11.6 | 11.1 | 8.5 | 8.6 | 11.3 | 11.1 | 11.8 | 11.5 |
Std Deviation (1e-4) | 3.6 | 3.0 | 3.1 | 2.6 | 2.2 | 2.0 | 2.1 | 2.0 | – | 2.4 | 2.1 | 2.2 | 4.1 | 4.1 | 3.7 | 1.9 | 2.4 | 3.0 | 2.6 | 2.8 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.02 | -0.19 | -0.19 | -0.14 | – | -0.22 | -0.24 | -0.14 | 0.12 | 0.12 | 0.06 | -0.32 | -0.3 | 0.08 | 0.06 | 0.14 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.6 | -8.7 | -9.7 | -11.7 | -13.7 | – | -12.5 | -12.0 | -12.9 | 0.6 | 1.4 | -0.5 | -3.9 | -4.5 | -4.3 |
Bud | -2.2 | -7.2 | -6.9 | -7.4 | -7.2 | – | -6.5 | -6.8 | -6.4 | 1.3 | 1.3 | 1.3 | -3.3 | -3.5 | -4.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -2.4 | -3.4 | -3.5 | -4.0 | -5.8 | – | -4.9 | -4.4 | -4.8 | 1.3 | 1.0 | 0 | -2.3 | -2.2 | -3.4 |
Cell Periphery | -2.4 | -6.7 | -8.1 | -9.1 | -10.4 | – | -8.4 | -6.7 | -7.8 | 5.3 | 5.3 | 4.3 | -3.2 | -5.7 | -1.0 |
Cytoplasm | 5.5 | 1.5 | 7.3 | 6.1 | 6.2 | – | 7.1 | 9.7 | 9.0 | 1.4 | 1.4 | 3.1 | 3.8 | 3.2 | 2.2 |
Endoplasmic Reticulum | 4.5 | 0.7 | -2.9 | -3.1 | -4.7 | – | -4.1 | -5.4 | -5.8 | -2.4 | -2.0 | -1.2 | -6.0 | -4.2 | -3.9 |
Endosome | -0.5 | 0.9 | -0.2 | -0.2 | -0.3 | – | -1.8 | 0 | -0.9 | 0 | 0 | 0 | -1.9 | -1.4 | -2.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.3 | -3.1 | -0.9 | 0.3 | -1.4 | – | 1.4 | 0.9 | -0.1 | 0 | 0 | 0 | 1.3 | 0.8 | 0.6 |
Nucleus | 0 | 3.7 | 3.1 | 5.8 | 3.5 | – | 3.9 | 4.1 | 3.5 | 0 | 0 | 0 | 5.7 | 6.1 | 4.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | -0.2 | – | 0 | 0 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.5 | 2.5 | 0 | -0.6 | -0.6 | -0.1 |
Vacuole | -2.7 | 9.5 | 6.6 | 8.2 | 8.8 | – | 7.2 | 6.0 | 6.5 | -5.4 | -5.6 | -5.0 | 3.5 | 5.0 | 3.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.8841 | 7.5308 | 8.9133 | 8.3518 | 7.2652 | 7.9898 | 10.3185 | 8.9997 | 9.6708 | 9.7014 | 7.437 | 8.8845 | 10.7396 | 9.5642 | 11.1783 | 9.5115 | 8.9551 | 10.2472 |
Actin | 0.0407 | 0.0084 | 0.0078 | 0.0057 | 0.0428 | 0.0163 | 0.0097 | 0.0018 | 0.0093 | 0.0041 | 0.0188 | 0.0054 | 0.036 | 0.0156 | 0.0584 | 0.0042 | 0.0569 | 0.0088 |
Bud | 0.0282 | 0.0318 | 0.2727 | 0.1193 | 0.0763 | 0.017 | 0.0116 | 0.024 | 0.1706 | 0.05 | 0.0228 | 0.0101 | 0.0164 | 0.0374 | 0.1656 | 0.0405 | 0.0419 | 0.0125 |
Bud Neck | 0.0295 | 0.0163 | 0.005 | 0.0005 | 0.0399 | 0.4428 | 0.0404 | 0.0094 | 0.008 | 0.0385 | 0.0018 | 0.4904 | 0.0361 | 0.0168 | 0.0068 | 0.0007 | 0.0026 | 0.4741 |
Bud Periphery | 0.1284 | 0.0548 | 0.4836 | 0.7233 | 0.4555 | 0.1464 | 0.0778 | 0.0763 | 0.4648 | 0.5383 | 0.4546 | 0.1257 | 0.0971 | 0.0684 | 0.4499 | 0.6358 | 0.5208 | 0.1088 |
Bud Site | 0.0814 | 0.3589 | 0.0673 | 0.0108 | 0.0425 | 0.0155 | 0.1341 | 0.4151 | 0.1225 | 0.0072 | 0.005 | 0.0124 | 0.063 | 0.358 | 0.0897 | 0.0021 | 0.0127 | 0.0076 |
Cell Periphery | 0.3345 | 0.1261 | 0.0539 | 0.0471 | 0.1403 | 0.1509 | 0.5055 | 0.2048 | 0.1096 | 0.1758 | 0.2611 | 0.2257 | 0.4811 | 0.1555 | 0.0702 | 0.1505 | 0.1261 | 0.2053 |
Cytoplasm | 0.0805 | 0.185 | 0.0191 | 0.0061 | 0.0286 | 0.0429 | 0.0452 | 0.1149 | 0.0153 | 0.0068 | 0.0268 | 0.0318 | 0.0452 | 0.1186 | 0.0257 | 0.0032 | 0.0264 | 0.0265 |
Cytoplasmic Foci | 0.0164 | 0.0103 | 0.0037 | 0.0002 | 0.0134 | 0.0025 | 0.018 | 0.0013 | 0.0077 | 0.0005 | 0.0052 | 0.0044 | 0.0087 | 0.0034 | 0.0135 | 0.0113 | 0.0065 | 0.0042 |
Eisosomes | 0.0017 | 0.0003 | 0.0001 | 0.0001 | 0.0012 | 0.0003 | 0.0006 | 0.0007 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0023 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0003 |
Endoplasmic Reticulum | 0.0389 | 0.0146 | 0.0046 | 0.0045 | 0.0119 | 0.0173 | 0.0208 | 0.0074 | 0.0054 | 0.0075 | 0.0497 | 0.006 | 0.026 | 0.0119 | 0.0102 | 0.008 | 0.0204 | 0.0251 |
Endosome | 0.0179 | 0.0162 | 0.0084 | 0.0012 | 0.0305 | 0.0057 | 0.0243 | 0.0014 | 0.0088 | 0.0062 | 0.0161 | 0.0067 | 0.0185 | 0.0062 | 0.0132 | 0.0164 | 0.0077 | 0.0152 |
Golgi | 0.0084 | 0.0029 | 0.0018 | 0.0002 | 0.0088 | 0.0031 | 0.0129 | 0.0005 | 0.0054 | 0.0009 | 0.0034 | 0.0032 | 0.01 | 0.0013 | 0.008 | 0.0206 | 0.0053 | 0.0079 |
Lipid Particles | 0.0229 | 0.0084 | 0.0015 | 0.0002 | 0.0077 | 0.0039 | 0.012 | 0.0041 | 0.0034 | 0.001 | 0.0024 | 0.0058 | 0.0119 | 0.0287 | 0.0047 | 0.0047 | 0.0066 | 0.0106 |
Mitochondria | 0.0132 | 0.0011 | 0.001 | 0.0005 | 0.0075 | 0.0057 | 0.0019 | 0.0002 | 0.0165 | 0.0012 | 0.0028 | 0.002 | 0.0196 | 0.0003 | 0.0049 | 0.0027 | 0.0019 | 0.0042 |
None | 0.0023 | 0.0015 | 0.0004 | 0.0001 | 0.0078 | 0.0003 | 0.0051 | 0.0036 | 0.0016 | 0.0002 | 0.0021 | 0.0001 | 0.0008 | 0.0011 | 0.0011 | 0.0001 | 0.0004 | 0.0002 |
Nuclear Periphery | 0.01 | 0.0021 | 0.0008 | 0.0014 | 0.0149 | 0.0019 | 0.0087 | 0.0011 | 0.0013 | 0.0013 | 0.0304 | 0.0005 | 0.0048 | 0.0023 | 0.0035 | 0.0015 | 0.0037 | 0.002 |
Nucleolus | 0.0026 | 0.0026 | 0.0062 | 0.0005 | 0.0028 | 0.0038 | 0.0006 | 0.0014 | 0.0006 | 0.0005 | 0.0005 | 0.001 | 0.0016 | 0.0024 | 0.0038 | 0.0003 | 0.0003 | 0.0012 |
Nucleus | 0.015 | 0.0048 | 0.0032 | 0.0018 | 0.0065 | 0.0046 | 0.0015 | 0.0031 | 0.0017 | 0.0014 | 0.0039 | 0.002 | 0.0019 | 0.0038 | 0.0054 | 0.0009 | 0.0016 | 0.0024 |
Peroxisomes | 0.0101 | 0.0002 | 0.0002 | 0 | 0.0018 | 0.0004 | 0.0016 | 0.0001 | 0.0013 | 0.0001 | 0.0388 | 0.0001 | 0.004 | 0.0001 | 0.0082 | 0.0013 | 0.0601 | 0.0017 |
Punctate Nuclear | 0.0086 | 0.0011 | 0.0007 | 0 | 0.0038 | 0.0002 | 0.0012 | 0.0002 | 0.0048 | 0 | 0.001 | 0.0001 | 0.0236 | 0.0003 | 0.0026 | 0.0001 | 0.0003 | 0.0008 |
Vacuole | 0.1032 | 0.1506 | 0.0558 | 0.0717 | 0.0512 | 0.1156 | 0.0646 | 0.1273 | 0.0398 | 0.153 | 0.0466 | 0.0652 | 0.0875 | 0.1655 | 0.0525 | 0.0913 | 0.0939 | 0.0779 |
Vacuole Periphery | 0.0056 | 0.0021 | 0.0023 | 0.0047 | 0.0043 | 0.003 | 0.0019 | 0.0014 | 0.0014 | 0.0054 | 0.0057 | 0.0011 | 0.0039 | 0.0022 | 0.0016 | 0.0038 | 0.0034 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 77.822 | 38.5109 | 32.8786 | 56.2867 | 63.0622 | 89.8161 | 50.9757 | 47.4749 | 54.2833 | 88.3385 |
Translational Efficiency | 0.5364 | 0.4441 | 0.4287 | 0.3826 | 0.4256 | 0.557 | 0.4274 | 0.3345 | 0.3931 | 0.4059 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
178 | 1135 | 585 | 1263 | 2088 | 1890 | 172 | 1672 | 2266 | 3025 | 757 | 2935 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1155.59 | 1092.94 | 1365.79 | 1444.30 | 1086.52 | 1118.46 | 1382.67 | 1476.31 | 1091.95 | 1108.88 | 1369.63 | 1462.54 |
Standard Deviation | 154.94 | 218.11 | 205.42 | 276.08 | 189.39 | 190.49 | 201.07 | 272.09 | 187.83 | 201.68 | 204.57 | 274.27 |
Intensity Change Log 2 | — | -0.080415 | 0.241106 | 0.321741 | — | 0.041799 | 0.347742 | 0.442281 | — | -0.019897 | 0.293766 | 0.381413 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.012582 | 0.005344 | 0.026910 | 0.010482 | 0.001775 | 0.004142 | 0.025879 | 0.007667 | 0.002624 | 0.004593 | 0.026676 | 0.008878 |
Bud Neck | 0.055769 | 0.083418 | 0.131622 | 0.043301 | 0.123256 | 0.118672 | 0.094800 | 0.060525 | 0.117955 | 0.105445 | 0.123256 | 0.053113 |
Bud Site | 0.302936* | 0.486578* | 0.429536* | 0.171735* | 0.277910* | 0.376838* | 0.472064* | 0.162043* | 0.279876* | 0.418013* | 0.439198* | 0.166214* |
Cell Periphery | 0.152932* | 0.167342* | 0.188974* | 0.548592* | 0.396605* | 0.363258* | 0.173747* | 0.533239* | 0.377464* | 0.289749* | 0.185515* | 0.539846* |
Cytoplasm | 0.127236* | 0.155989* | 0.046245 | 0.100019* | 0.051819 | 0.049233 | 0.038835 | 0.112078* | 0.057743 | 0.089289 | 0.044561 | 0.106889* |
Cytoplasmic Foci | 0.012144 | 0.007447 | 0.005987 | 0.001823 | 0.000888 | 0.003193 | 0.005224 | 0.004177 | 0.001772 | 0.004789 | 0.005814 | 0.003164 |
Eisosomes | 0.001601 | 0.000527 | 0.002768 | 0.002351 | 0.000574 | 0.000724 | 0.002979 | 0.002202 | 0.000655 | 0.000650 | 0.002816 | 0.002266 |
Endoplasmic Reticulum | 0.016652 | 0.009297 | 0.046177 | 0.018944 | 0.027633 | 0.015036 | 0.058466 | 0.024829 | 0.026771 | 0.012883 | 0.048970 | 0.022297 |
Endosome | 0.010380 | 0.006449 | 0.005714 | 0.002885 | 0.004387 | 0.004965 | 0.005237 | 0.002477 | 0.004858 | 0.005522 | 0.005606 | 0.002653 |
Golgi | 0.010374 | 0.001437 | 0.001542 | 0.002571 | 0.000444 | 0.001309 | 0.000934 | 0.001919 | 0.001224 | 0.001357 | 0.001404 | 0.002200 |
Lipid Particles | 0.007210 | 0.001167 | 0.015174 | 0.019716 | 0.000814 | 0.000564 | 0.013474 | 0.017579 | 0.001316 | 0.000790 | 0.014788 | 0.018498 |
Mitochondria | 0.002156 | 0.000819 | 0.000215 | 0.000851 | 0.000934 | 0.003505 | 0.000118 | 0.000885 | 0.001030 | 0.002497 | 0.000193 | 0.000870 |
Mitotic Spindle | 0.002331 | 0.000160 | 0.000228 | 0.000762 | 0.000164 | 0.000236 | 0.000182 | 0.000544 | 0.000334 | 0.000207 | 0.000217 | 0.000638 |
None | 0.000043 | 0.000123 | 0.000021 | 0.000088 | 0.000048 | 0.000034 | 0.000012 | 0.000090 | 0.000048 | 0.000067 | 0.000019 | 0.000089 |
Nuclear Periphery | 0.000076 | 0.000031 | 0.000127 | 0.000815 | 0.000113 | 0.000060 | 0.000155 | 0.000989 | 0.000110 | 0.000049 | 0.000134 | 0.000914 |
Nuclear Periphery Foci | 0.001515 | 0.000339 | 0.003943 | 0.001384 | 0.000396 | 0.000260 | 0.004643 | 0.001258 | 0.000484 | 0.000290 | 0.004102 | 0.001312 |
Nucleolus | 0.000050 | 0.000683 | 0.000029 | 0.000067 | 0.000152 | 0.000093 | 0.000016 | 0.000092 | 0.000144 | 0.000314 | 0.000026 | 0.000081 |
Nucleus | 0.004813 | 0.001172 | 0.001582 | 0.001366 | 0.000886 | 0.000726 | 0.001941 | 0.001658 | 0.001194 | 0.000893 | 0.001664 | 0.001532 |
Peroxisomes | 0.001338 | 0.000977 | 0.000525 | 0.001420 | 0.000549 | 0.000523 | 0.000336 | 0.002481 | 0.000611 | 0.000693 | 0.000482 | 0.002025 |
Vacuole | 0.274513* | 0.067788 | 0.092095 | 0.067300 | 0.107133 | 0.052290 | 0.100795 | 0.061866 | 0.120281 | 0.058105 | 0.094072 | 0.064205 |
Vacuole Periphery | 0.003349 | 0.002912 | 0.000584 | 0.003528 | 0.003520 | 0.004337 | 0.000163 | 0.001401 | 0.003506 | 0.003803 | 0.000489 | 0.002316 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.09 | -4.15 | 0.76 | -4.26 | 5.69 | -4.93 | -5.94 | -6.36 | -3.42 | 4.35 | -4.92 | -10.68 | -8.48 | -5.45 | 7.52 |
Bud Neck | -3.06 | -6.33 | 1.17 | 7.41 | 9.72 | 0.73 | 2.57 | 11.36 | 10.39 | 2.77 | 2.35 | -0.38 | 13.75 | 13.04 | 9.25 |
Bud Site | -6.75 | -4.35 | 4.60 | 25.64* | 15.93* | -9.36 | -6.50 | 12.41 | 21.70* | 10.66 | -14.89 | -10.70 | 13.73 | 32.48* | 19.78* |
Cell Periphery | -1.06 | -1.95 | -20.02 | -31.06* | -26.85* | 3.04 | 11.86 | -11.95 | -14.58 | -18.60 | 9.24 | 17.48 | -16.80 | -28.62 | -33.88* |
Cytoplasm | -1.70 | 6.24 | 2.25 | 6.75 | -8.38 | 0.69 | 1.37 | -11.25 | -11.74 | -8.06 | -7.78 | 2.76 | -11.42 | -3.89 | -12.53 |
Cytoplasmic Foci | 1.42 | 1.68 | 3.27 | 5.14 | 4.07 | -3.58 | -3.78 | -5.12 | -1.17 | 1.25 | -4.82 | -4.86 | -2.98 | 2.35 | 3.27 |
Eisosomes | 3.86 | -3.56 | -2.62 | -10.25 | 1.47 | -2.44 | -6.08 | -12.84 | -11.15 | 1.78 | 0.02 | -11.06 | -15.18 | -15.04 | 2.45 |
Endoplasmic Reticulum | 1.27 | -4.05 | -0.52 | -4.03 | 4.79 | 5.79 | -2.48 | 0.98 | -3.90 | 2.69 | 7.20 | -4.22 | 1.95 | -5.31 | 5.13 |
Endosome | 1.60 | 1.84 | 3.15 | 3.91 | 3.42 | -0.96 | -0.92 | 4.27 | 3.96 | 2.59 | -1.31 | -1.51 | 4.74 | 5.42 | 4.93 |
Golgi | 1.92 | 1.90 | 1.65 | -1.76 | -1.65 | -3.03 | -2.73 | -2.70 | -1.00 | -1.75 | -0.44 | -0.55 | -1.89 | -1.87 | -1.90 |
Lipid Particles | 2.81 | -2.72 | -4.32 | -8.81 | -1.47 | 2.30 | -4.32 | -10.19 | -10.33 | -1.07 | 2.51 | -7.36 | -13.11 | -13.58 | -1.58 |
Mitochondria | 0.85 | 1.29 | 0.85 | -0.03 | -3.65 | -3.33 | 5.18 | 0.23 | 3.40 | -5.21 | -2.90 | 4.47 | 0.72 | 3.34 | -5.87 |
Mitotic Spindle | 1.42 | 1.35 | 0.94 | -2.22 | -1.82 | -1.96 | -0.84 | -12.06 | -6.56 | -9.69 | 1.01 | 0.59 | -1.95 | -3.43 | -2.94 |
None | -2.20 | 0.83 | -1.47 | 1.37 | -5.06 | 0.90 | 2.22 | -2.07 | -4.14 | -5.73 | -1.22 | 1.85 | -2.45 | -1.47 | -6.89 |
Nuclear Periphery | 4.28 | -2.97 | -7.58 | -8.17 | -6.96 | 5.27 | -1.83 | -14.76 | -15.78 | -13.13 | 6.99 | -2.17 | -14.94 | -16.26 | -14.10 |
Nuclear Periphery Foci | 5.29 | -4.07 | 0.42 | -8.14 | 4.43 | 5.00 | -4.46 | -10.40 | -12.17 | 3.59 | 6.05 | -7.42 | -11.06 | -14.10 | 5.72 |
Nucleolus | -1.35 | 2.03 | -1.35 | 1.32 | -6.93 | 5.72 | 15.75 | 6.51 | 0.14 | -14.20 | -1.06 | 14.49 | 7.74 | 1.33 | -12.87 |
Nucleus | 1.68 | 1.48 | 1.64 | -0.65 | 2.34 | 2.17 | -3.13 | -7.67 | -9.85 | 0.97 | 1.53 | -1.91 | -0.82 | -6.49 | 2.01 |
Peroxisomes | 0.29 | 2.20 | -0.28 | -0.61 | -4.80 | 0.33 | 2.06 | -7.29 | -7.44 | -7.93 | -0.96 | 1.29 | -8.11 | -5.70 | -8.65 |
Vacuole | 8.40 | 7.06 | 8.51 | 0.13 | 3.92 | 10.37 | -0.41 | 9.15 | -2.49 | 3.04 | 11.96 | 2.28 | 11.51 | -1.91 | 5.32 |
Vacuole Periphery | 0.23 | 2.00 | -0.23 | -0.69 | -3.18 | -1.33 | 10.24 | 5.04 | 5.02 | -4.68 | -0.65 | 8.87 | 2.16 | 2.64 | -4.18 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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