Standard name
Human Ortholog
Description Hydrophilin essential in desiccation-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; GRE1 has a paralog, SIP18, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0.05 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.91 – 0.99 0.98 0.95 0.95 – 0.85 0.86 0.78 0.81 1.0 1.0 1.0 – 0.97 0.96 0.71 0.87 0.75 0.83 0.83 0.85
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.18 – 0 0 0 0 – 0.24 0.21 0.33 0.27 0 0 0 – 0 0 0.14 0 0.14 0.09 0.06 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 1 0 0 1 6 5
Bud 0 0 – 0 1 1 0 – 6 0 5 8 0 1 0 – 0 1 1 0 0 1 9 14
Bud Neck 1 0 – 5 8 6 14 – 9 9 8 8 0 0 0 – 3 1 1 0 0 1 3 4
Bud Site 0 0 – 0 0 0 0 – 0 0 3 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 – 1 0 1 0 – 0 1 0 2 1 1 2 – 5 2 1 0 0 3 5 2
Cytoplasm 157 20 – 137 290 121 317 – 248 264 262 298 399 606 700 – 163 160 123 47 34 210 432 511
Endoplasmic Reticulum 1 0 – 0 1 0 0 – 0 0 0 0 1 0 0 – 10 11 1 0 0 0 1 1
Endosome 1 0 – 0 1 0 2 – 0 1 0 0 0 0 0 – 1 1 3 0 0 0 1 3
Golgi 1 0 – 0 0 0 0 – 0 0 0 0 0 1 1 – 0 0 3 0 0 1 0 2
Mitochondria 1 4 – 5 0 5 9 – 69 64 110 98 0 0 1 – 3 2 24 2 6 21 29 25
Nucleus 1 0 – 1 4 1 1 – 3 4 2 2 0 0 0 – 0 1 0 0 0 1 0 1
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0
Nucleolus 0 0 – 0 1 0 1 – 0 0 2 1 0 0 1 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 – 0 1 0 0 – 0 1 0 0 0 0 1 – 0 2 0 0 0 0 0 1
SpindlePole 1 0 – 0 2 1 6 – 6 9 9 11 0 0 0 – 1 0 2 0 0 2 11 6
Vac/Vac Membrane 1 0 – 0 1 0 0 – 0 2 0 1 0 0 1 – 1 0 3 2 0 4 5 11
Unique Cell Count 163 22 139 295 128 334 291 307 334 369 400 608 702 168 166 174 55 46 252 519 603
Labelled Cell Count 166 24 149 310 136 350 341 355 401 429 401 609 707 187 182 174 55 46 252 519 603


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.4 – 4.1 5.1 3.1 4.1 – 3.4 3.7 3.3 3.6 4.1 4.3 4.9 4.7 5.1 5.4 3.8 6.2 4.5
Std Deviation (1e-4) 1.2 0.6 – 1.4 2.1 0.9 1.6 – 1.5 1.6 1.1 1.6 0.9 1.0 5.8 0.9 1.0 1.6 1.2 27.0 14.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5944 -1.1128 -1.4095 -0.901 -1.5176 -1.3534 -0.3978 0.7225 -0.2439 -0.3219 -0.0919 0.6827 2.6469 2.7979 2.3743 3.2122 2.1685 2.0142
Actin 0.005 0.0001 0.0015 0.0001 0.002 0.0003 0.0343 0.0005 0.0027 0.0003 0.0011 0.0074 0.0442 0.0001 0.0024 0.0002 0.0713 0.0059
Bud 0.001 0 0.0005 0 0.0026 0.0001 0.0003 0.0009 0.0016 0.0001 0 0.0002 0.0011 0.0087 0.001 0.0002 0.0029 0.0003
Bud Neck 0.0013 0 0.008 0.0001 0.0025 0.0001 0.0022 0.0002 0.0001 0.0005 0.0006 0.0007 0.0009 0.0001 0.0003 0.0004 0.0003 0.0006
Bud Periphery 0.003 0 0.0005 0 0.0026 0.0001 0.0006 0.0006 0.0027 0.0001 0 0.0002 0.001 0.0053 0.0017 0.0001 0.0086 0.0007
Bud Site 0.0033 0 0.0122 0 0.0096 0 0.0016 0.0075 0.0022 0.0003 0.0001 0.0003 0.0021 0.0028 0.0093 0.0021 0.0016 0.0001
Cell Periphery 0.0005 0 0.0002 0 0.0003 0 0.0003 0.0003 0.0002 0.0002 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0008 0.0001
Cytoplasm 0.0248 0.0295 0.0178 0.1185 0.0429 0.0462 0.146 0.2533 0.1558 0.1744 0.2608 0.2469 0.2363 0.2634 0.2983 0.3994 0.3413 0.2919
Cytoplasmic Foci 0.0065 0.001 0.0055 0.0124 0.0076 0.0049 0.0112 0.0105 0.0044 0.009 0.0024 0.0159 0.0281 0.0013 0.0021 0.0068 0.0034 0.0093
Eisosomes 0.001 0.0002 0.0002 0.0001 0.0002 0.0001 0.0008 0.0002 0.0003 0.0002 0.0001 0.0004 0.0004 0.0001 0.0001 0.0001 0.0018 0
Endoplasmic Reticulum 0.0009 0.0004 0.0008 0.0012 0.0014 0.0002 0.002 0.0007 0.0007 0.0019 0.0008 0.0009 0.002 0.0003 0.0015 0.0009 0.0014 0.0009
Endosome 0.005 0.0001 0.0004 0.0077 0.0067 0.0004 0.009 0.0017 0.0005 0.0041 0.0065 0.0021 0.017 0.0001 0.0006 0.0226 0.003 0.0117
Golgi 0.0018 0 0.0001 0.0002 0.0005 0.0001 0.0044 0.0001 0.0001 0.0008 0.0003 0.0007 0.0113 0 0.0001 0.0028 0.009 0.0018
Lipid Particles 0.0068 0.0003 0.0005 0.0013 0.0009 0.0001 0.0087 0.0012 0.0003 0.0222 0.0007 0.005 0.0089 0.0001 0.0001 0.0012 0.0007 0.0003
Mitochondria 0.0016 0.0001 0.0002 0.0006 0.0014 0.0001 0.0056 0.0002 0.0053 0.0394 0.0008 0.0005 0.0063 0.0001 0.0008 0.0003 0.0636 0.0004
None 0.9236 0.9673 0.9435 0.8526 0.8898 0.9448 0.7513 0.7167 0.8189 0.7371 0.625 0.6952 0.612 0.7159 0.6718 0.5526 0.4875 0.6673
Nuclear Periphery 0.0019 0.0002 0.0005 0.002 0.0136 0.0002 0.0056 0.0004 0.0004 0.0013 0.0311 0.0021 0.0074 0.0001 0.0059 0.0004 0.0004 0.0004
Nucleolus 0.0013 0.0001 0.0005 0.0002 0.0013 0.0001 0.0006 0.0002 0.0011 0.0006 0.0082 0.001 0.0007 0.0001 0.0001 0.0002 0 0.0001
Nucleus 0.0013 0.0002 0.0009 0.0008 0.0067 0.0003 0.0044 0.0018 0.0014 0.0012 0.036 0.0118 0.0032 0.0009 0.0017 0.0018 0.0007 0.0026
Peroxisomes 0.0032 0 0.0002 0.0004 0.0005 0.0002 0.0059 0.0001 0.0004 0.001 0.0001 0.0041 0.0115 0 0.0003 0.0003 0.0004 0.0001
Punctate Nuclear 0.0031 0.0003 0.0057 0.001 0.005 0.0015 0.0034 0.0012 0.0005 0.0011 0.0239 0.004 0.0033 0.0002 0.0004 0.0003 0.0002 0.0007
Vacuole 0.0022 0.0001 0.0003 0.0008 0.0012 0.0001 0.0013 0.0017 0.0004 0.0032 0.0006 0.0004 0.0014 0.0002 0.0005 0.0069 0.0007 0.004
Vacuole Periphery 0.0007 0 0 0.0003 0.0007 0 0.0006 0.0001 0.0001 0.0008 0.0007 0.0001 0.0009 0 0.0008 0.0004 0.0005 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1003 353 1901 79 1366 1673 475 1489 2369 2026 2376 1568

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 570.50 616.56 734.43 875.06 645.85 741.95 811.61 752.59 613.95 720.10 749.86 758.76
Standard Deviation 64.02 110.03 92.72 193.20 75.20 93.43 101.50 91.75 79.89 107.61 99.45 102.92
Intensity Change Log 2 — 0.112014 0.364398 0.617155 — 0.200123 0.329588 0.220665 — 0.159467 0.346019 0.420232

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000159 0.000625 0.000126 0.000366 0.000066 0.000246 0.000248 0.000191 0.000106 0.000312 0.000151 0.000200
Bud Neck 0.023426 0.029858 0.005599 0.003549 0.014021 0.033316 0.001801 0.002887 0.018003 0.032713 0.004840 0.002921
Bud Site 0.006455 0.015060 0.006826 0.005561 0.002952 0.016737 0.002985 0.019358 0.004435 0.016445 0.006058 0.018663
Cell Periphery 0.000169 0.000177 0.000090 0.000261 0.000172 0.000168 0.000162 0.000131 0.000170 0.000169 0.000104 0.000138
Cytoplasm 0.391059* 0.404497* 0.384638* 0.342857* 0.488565* 0.492047* 0.386897* 0.538446* 0.447283* 0.476793* 0.385090* 0.528592*
Cytoplasmic Foci 0.308286* 0.249797* 0.006000 0.043617 0.236456* 0.157669 0.020548 0.035684 0.266868* 0.173721 0.008908 0.036084
Eisosomes 0.000272 0.000139 0.000016 0.000121 0.000190 0.000119 0.000062 0.000048 0.000224 0.000123 0.000025 0.000052
Endoplasmic Reticulum 0.001023 0.000954 0.001836 0.004108 0.000970 0.000752 0.003599 0.001073 0.000993 0.000787 0.002188 0.001226
Endosome 0.012626 0.034045 0.000678 0.005842 0.009734 0.011922 0.002924 0.002528 0.010958 0.015777 0.001127 0.002695
Golgi 0.003201 0.003385 0.000058 0.000191 0.002002 0.003173 0.000083 0.000217 0.002510 0.003210 0.000063 0.000216
Lipid Particles 0.022879 0.010075 0.000453 0.003351 0.014079 0.005540 0.002318 0.001280 0.017805 0.006330 0.000826 0.001385
Mitochondria 0.015129 0.006515 0.000666 0.000322 0.006414 0.007691 0.000305 0.000659 0.010104 0.007486 0.000594 0.000642
Mitotic Spindle 0.000246 0.004645 0.002460 0.031249 0.002960 0.008885 0.001357 0.006743 0.001811 0.008147 0.002240 0.007978
None 0.003896 0.008767 0.004923 0.002414 0.002883 0.005591 0.007276 0.003345 0.003312 0.006145 0.005393 0.003298
Nuclear Periphery 0.001221 0.001065 0.001126 0.001537 0.000683 0.000849 0.001265 0.000946 0.000910 0.000887 0.001154 0.000976
Nuclear Periphery Foci 0.000281 0.001559 0.000490 0.002504 0.000366 0.000261 0.002212 0.000503 0.000330 0.000487 0.000834 0.000604
Nucleolus 0.001911 0.002744 0.000312 0.000417 0.001523 0.001538 0.000712 0.000437 0.001687 0.001748 0.000392 0.000436
Nucleus 0.157062* 0.128109 0.565250* 0.355651* 0.179430* 0.190348* 0.478996* 0.298387* 0.169960* 0.179504* 0.548006* 0.301272*
Peroxisomes 0.011966 0.016196 0.000127 0.000571 0.004978 0.006744 0.000338 0.000540 0.007936 0.008391 0.000169 0.000541
Vacuole 0.037209 0.078895 0.018145 0.195283 0.030731 0.054723 0.085748 0.085948 0.033474 0.058934 0.031660 0.091456
Vacuole Periphery 0.001524 0.002892 0.000183 0.000230 0.000825 0.001681 0.000162 0.000646 0.001121 0.001892 0.000179 0.000625

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.94 -0.34 -3.77 1.04 -3.99 -4.42 -7.42 -3.39 1.12 3.40 -4.52 -4.93 -2.58 2.12 0.27
Bud Neck -1.06 7.69 9.07 5.81 2.49 -7.17 7.70 7.40 13.07 -1.58 -6.16 9.03 11.99 14.31 3.23
Bud Site -2.80 -0.24 0.65 3.41 1.04 -7.47 -1.22 -5.04 0.93 -4.07 -7.08 -1.67 -4.26 1.06 -3.26
Cell Periphery -0.27 6.63 -2.08 -1.74 -4.44 0.37 0.27 5.75 4.39 4.02 0.20 7.52 5.89 4.39 -1.32
Cytoplasm -0.42 2.94 4.37 4.12 3.27 -0.35 11.35 1.20 1.55 -10.40 -3.45 11.90 -2.24 0.95 -12.46
Cytoplasmic Foci 4.46 43.22 31.33 17.45 -4.30 10.61 36.76 35.06 25.50 -5.74 14.79 57.41 50.36 30.54 -14.80
Eisosomes 6.42 13.70 6.50 0.70 -9.16 6.02 11.32 15.05 8.04 3.95 8.92 20.49 17.58 9.33 -11.01
Endoplasmic Reticulum -0.09 -5.41 -4.63 -4.64 -3.45 0.83 -9.79 -2.29 -5.43 9.78 0.94 -9.53 -4.05 -6.76 7.16
Endosome -4.76 11.69 6.45 6.65 -7.30 -1.79 7.57 8.89 10.61 3.12 -3.76 15.02 13.00 12.42 -6.73
Golgi 0.04 6.92 6.75 5.10 -2.34 -2.09 7.14 6.77 6.51 -1.20 -1.34 9.92 9.36 7.60 -1.77
Lipid Particles 4.79 11.48 9.83 3.67 -6.34 7.43 11.05 12.44 8.06 4.43 9.99 16.56 16.10 9.16 -5.34
Mitochondria 3.52 6.38 6.50 4.80 1.84 -0.81 4.74 4.55 7.92 -1.66 1.88 7.80 7.85 8.88 0.63
Mitotic Spindle -2.56 -3.10 -1.45 -1.06 -1.25 -3.02 0.15 -1.35 1.82 -1.31 -4.10 -0.79 -2.71 1.29 -2.21
None -1.62 -0.77 0.44 1.74 0.98 -3.22 -3.76 -0.39 2.95 3.45 -3.13 -3.54 0.57 3.48 4.35
Nuclear Periphery 0.28 -0.34 -2.27 -2.79 -4.42 -0.43 -4.63 -3.50 -1.94 2.75 0.08 -2.32 -2.55 -2.29 -0.93
Nuclear Periphery Foci -1.61 -4.66 -6.78 -1.43 -5.56 1.10 -8.62 -2.96 -5.83 7.91 -1.00 -8.34 -5.84 -1.76 4.28
Nucleolus -0.29 3.22 3.23 5.80 0.16 0.38 1.94 2.45 7.38 2.28 0.78 3.88 3.96 9.22 0.60
Nucleus 2.05 -48.86 -6.65 -7.16 8.92 -1.55 -21.75 -17.29 -15.55 11.09 -1.77 -55.33 -20.58 -17.79 26.83
Peroxisomes -1.69 10.38 10.14 7.03 -3.12 -2.47 9.94 9.54 11.47 -0.70 -0.56 13.93 13.30 13.12 -2.08
Vacuole -6.66 2.60 -8.75 -7.03 -8.97 -8.51 -11.42 -20.88 -15.01 -0.40 -10.19 -6.28 -21.99 -15.49 -17.21
Vacuole Periphery -1.74 7.23 7.29 3.81 -0.32 -3.51 6.09 1.33 3.58 -1.03 -3.15 8.84 2.52 4.29 -0.96
Standard name
Human Ortholog
Description Hydrophilin essential in desiccation-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; GRE1 has a paralog, SIP18, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gre1

Gre1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gre1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available