Standard name | |
---|---|
Human Ortholog | |
Description | UDP-glucose:dolichyl-phosphate glucosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum; human ortholog ALG5 can partially complement yeast alg5 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 7 | 9 | 15 | 16 | 4 | 9 | – | 2 | 5 | 12 | 3 | 7 | 11 | 0 | 12 | 1 | 0 | 2 | 3 | 1 | 3 | 3 |
Bud | 0 | 2 | 1 | 2 | 9 | 20 | 6 | 12 | – | 19 | 20 | 16 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 7 | 19 | 34 | 21 | 17 | 14 | – | 28 | 16 | 18 | 8 | 37 | 64 | 8 | 30 | 6 | 0 | 0 | 0 | 0 | 0 | 2 |
Cytoplasm | 10 | 23 | 13 | 56 | 94 | 121 | 108 | 139 | – | 171 | 137 | 190 | 25 | 32 | 54 | 4 | 24 | 20 | 1 | 4 | 1 | 2 | 14 | 20 |
Endoplasmic Reticulum | 72 | 144 | 172 | 140 | 368 | 254 | 287 | 187 | – | 205 | 87 | 159 | 122 | 275 | 275 | 0 | 2 | 1 | 99 | 156 | 196 | 129 | 215 | 206 |
Endosome | 0 | 1 | 1 | 4 | 4 | 3 | 2 | 9 | – | 7 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 5 | 1 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 2 | 2 | 25 | 68 | 111 | 192 | 311 | 229 | – | 211 | 226 | 327 | 1 | 0 | 3 | 3 | 10 | 3 | 6 | 2 | 3 | 5 | 10 | 6 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 9 | 5 | 11 | – | 7 | 2 | 6 | 4 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 2 | 4 | 14 | 8 | 14 | 13 | 42 | 27 | – | 28 | 13 | 12 | 0 | 3 | 6 | 2 | 3 | 0 | 14 | 0 | 0 | 1 | 2 | 1 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 5 | – | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 11 | 8 | 5 | 38 | 73 | 73 | 181 | 182 | – | 182 | 101 | 90 | 12 | 2 | 13 | 0 | 6 | 13 | 5 | 3 | 1 | 4 | 12 | 20 |
Unique Cell Count | 97 | 164 | 198 | 280 | 566 | 573 | 708 | 680 | 686 | 491 | 667 | 153 | 317 | 366 | 16 | 87 | 44 | 134 | 174 | 213 | 148 | 266 | 272 | |
Labelled Cell Count | 101 | 187 | 246 | 346 | 722 | 723 | 964 | 824 | 860 | 608 | 835 | 176 | 357 | 429 | 18 | 96 | 49 | 134 | 174 | 213 | 148 | 266 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.8 | 11.6 | 7.3 | 7.3 | 7.1 | 6.4 | 5.9 | 6.3 | – | 6.0 | 5.9 | 5.4 | 9.5 | 9.5 | 9.5 | 13.6 | 14.3 | 14.5 | 10.7 | 10.4 | 10.2 |
Std Deviation (1e-4) | 1.9 | 1.7 | 1.0 | 1.2 | 1.3 | 1.2 | 1.2 | 1.2 | – | 1.2 | 1.2 | 1.1 | 1.2 | 1.2 | 1.2 | 2.3 | 2.4 | 2.8 | 1.4 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.0 | -0.03 | -0.18 | -0.3 | -0.21 | – | -0.28 | -0.31 | -0.42 | 0.39 | 0.39 | 0.39 | 0.91 | 0.98 | 0.99 | 0.56 | 0.51 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | -0.6 | -0.5 | 0 | 0 | – | 0 | 0 | 0 | 0 | -0.9 | -0.3 | 0 | 3.2 | 0 |
Bud | 0 | 0 | 2.2 | 0 | 0 | – | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.5 | 1.3 | 0.1 | -0.9 | 0 | – | 0.3 | -0.2 | -0.6 | 0.8 | 3.2 | 4.8 | 0 | 7.2 | 0 |
Cytoplasm | 4.1 | 3.5 | 4.7 | 3.2 | 4.5 | – | 5.6 | 6.1 | 6.4 | 2.9 | 1.4 | 2.9 | 0 | 4.9 | 6.8 |
Endoplasmic Reticulum | -8.3 | -5.8 | -10.4 | -11.5 | -15.0 | – | -14.3 | -17.0 | -16.0 | -1.8 | 0 | -3.3 | -8.4 | -13.5 | -11.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.2 | 2.2 | 5.6 | 8.1 | 5.7 | – | 5.1 | 8.2 | 9.2 | -4.2 | -6.5 | -6.2 | 0 | -0.3 | -1.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -2.2 | -3.0 | -3.2 | -0.6 | -1.8 | – | -1.7 | -2.7 | -3.8 | -3.4 | -3.8 | -3.3 | 0 | -1.2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 4.2 | 4.1 | 4.1 | 7.1 | 7.3 | – | 7.3 | 5.9 | 4.3 | 2.3 | 0 | 0.7 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.8007 | 5.6543 | 5.4401 | 5.0327 | 5.0952 | 5.1977 | 5.7295 | 6.7968 | 6.2072 | 5.9658 | 5.6483 | 6.4272 | 4.0434 | 4.8179 | 4.5087 | 4.346 | 3.754 | 4.2672 |
Actin | 0.0053 | 0.0007 | 0.004 | 0.0005 | 0.0175 | 0.0007 | 0.0148 | 0.0004 | 0.0145 | 0.01 | 0.0365 | 0.0003 | 0.0023 | 0 | 0.0024 | 0.0002 | 0.0257 | 0.0026 |
Bud | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0022 | 0.0004 | 0.0015 | 0.0006 | 0.0007 | 0.0006 | 0.0024 | 0.0001 | 0.0003 | 0.0006 | 0.0009 | 0.0005 | 0.0002 | 0 | 0.0008 | 0.007 | 0.0006 | 0.0003 |
Bud Periphery | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Bud Site | 0.0005 | 0.0116 | 0.0031 | 0 | 0.0004 | 0 | 0.0017 | 0.0004 | 0.0004 | 0.0008 | 0.001 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0004 | 0 |
Cell Periphery | 0.0006 | 0.0025 | 0.0009 | 0.0004 | 0.0006 | 0.0006 | 0.0011 | 0.0013 | 0.0013 | 0.0037 | 0.004 | 0.0005 | 0.0005 | 0.0003 | 0.0007 | 0.0005 | 0.0009 | 0.0002 |
Cytoplasm | 0.0712 | 0.1 | 0.0374 | 0.0787 | 0.0741 | 0.05 | 0.0265 | 0.0687 | 0.0458 | 0.0517 | 0.0528 | 0.0406 | 0.0176 | 0.0235 | 0.0348 | 0.0409 | 0.014 | 0.0285 |
Cytoplasmic Foci | 0.0119 | 0.0028 | 0.0065 | 0.0062 | 0.0032 | 0.0033 | 0.0062 | 0.0017 | 0.0079 | 0.0134 | 0.0107 | 0.001 | 0.005 | 0.001 | 0.002 | 0.0004 | 0.0099 | 0.0009 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0006 | 0 | 0.0002 | 0.0003 | 0.0008 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.7921 | 0.8101 | 0.8605 | 0.8805 | 0.7846 | 0.9018 | 0.8613 | 0.8912 | 0.8697 | 0.8269 | 0.7646 | 0.9354 | 0.9198 | 0.9434 | 0.926 | 0.9326 | 0.8276 | 0.9376 |
Endosome | 0.0332 | 0.0109 | 0.0182 | 0.0035 | 0.0167 | 0.0116 | 0.0142 | 0.0041 | 0.0164 | 0.0177 | 0.0165 | 0.0049 | 0.012 | 0.0023 | 0.0072 | 0.0025 | 0.0174 | 0.0055 |
Golgi | 0.0036 | 0.001 | 0.005 | 0.0002 | 0.005 | 0.0009 | 0.004 | 0.0008 | 0.0053 | 0.0031 | 0.012 | 0.0004 | 0.0022 | 0.0002 | 0.0011 | 0.0001 | 0.0067 | 0.0016 |
Lipid Particles | 0.0145 | 0.0046 | 0.01 | 0.0008 | 0.0369 | 0.0024 | 0.0069 | 0.008 | 0.0106 | 0.0209 | 0.0255 | 0.0034 | 0.0075 | 0.0169 | 0.0076 | 0.0008 | 0.0219 | 0.001 |
Mitochondria | 0.0016 | 0.0023 | 0.0132 | 0.0002 | 0.0043 | 0.0008 | 0.0026 | 0.0064 | 0.001 | 0.0007 | 0.0066 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0028 | 0.0003 |
None | 0.0116 | 0.0161 | 0.0091 | 0.0112 | 0.0044 | 0.0019 | 0.0226 | 0.0007 | 0.0033 | 0.0177 | 0.039 | 0.0038 | 0.0052 | 0.0002 | 0.0009 | 0.0003 | 0.0072 | 0.0004 |
Nuclear Periphery | 0.0281 | 0.0167 | 0.0114 | 0.0075 | 0.0321 | 0.0145 | 0.016 | 0.0054 | 0.0091 | 0.0076 | 0.0049 | 0.005 | 0.0188 | 0.0085 | 0.0094 | 0.0101 | 0.0304 | 0.0147 |
Nucleolus | 0.0024 | 0.007 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0 |
Nucleus | 0.0052 | 0.0059 | 0.002 | 0.0026 | 0.0027 | 0.0033 | 0.0024 | 0.0007 | 0.003 | 0.0014 | 0.0015 | 0.0007 | 0.001 | 0.0005 | 0.0011 | 0.0008 | 0.0023 | 0.0017 |
Peroxisomes | 0.0031 | 0.0003 | 0.0078 | 0 | 0.0021 | 0.0002 | 0.0074 | 0.0066 | 0.0039 | 0.0165 | 0.0168 | 0 | 0.0016 | 0 | 0.0002 | 0 | 0.0099 | 0 |
Punctate Nuclear | 0.0059 | 0.0014 | 0.0007 | 0.0042 | 0.0042 | 0.004 | 0.0025 | 0.0001 | 0.0024 | 0.0021 | 0.0014 | 0.0001 | 0.001 | 0.0002 | 0.0007 | 0.0001 | 0.0144 | 0.0014 |
Vacuole | 0.0054 | 0.0043 | 0.0068 | 0.0024 | 0.004 | 0.0028 | 0.0045 | 0.003 | 0.0041 | 0.0038 | 0.0034 | 0.0029 | 0.0034 | 0.0026 | 0.0039 | 0.003 | 0.0041 | 0.0027 |
Vacuole Periphery | 0.0012 | 0.001 | 0.0008 | 0.0003 | 0.0045 | 0.0005 | 0.0009 | 0.0002 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.0009 | 0.0002 | 0.0005 | 0.0005 | 0.0018 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.5249 | 46.129 | 23.3054 | 37.0386 | 30.6051 | 34.0165 | 41.4311 | 36.5029 | 32.6893 | 42.4938 |
Translational Efficiency | 1.1836 | 0.9021 | 1.1317 | 0.5649 | 1.0424 | 0.9358 | 0.7924 | 0.6274 | 0.7105 | 0.7248 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2240 | 1879 | 283 | 125 | 1615 | 1519 | 1233 | 1601 | 3855 | 3398 | 1516 | 1726 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 940.89 | 1079.05 | 1358.42 | 1257.36 | 943.48 | 999.14 | 1204.43 | 1191.98 | 941.98 | 1043.33 | 1233.18 | 1196.71 |
Standard Deviation | 124.35 | 162.91 | 144.36 | 165.14 | 141.47 | 135.43 | 142.25 | 175.34 | 131.80 | 156.38 | 154.75 | 175.44 |
Intensity Change Log 2 | — | 0.197664 | 0.529832 | 0.418300 | — | 0.082695 | 0.352287 | 0.337296 | — | 0.141245 | 0.443667 | 0.378311 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000484 | 0.001072 | 0.000317 | 0.003436 | 0.000447 | 0.001524 | 0.000978 | 0.003381 | 0.000468 | 0.001274 | 0.000855 | 0.003385 |
Bud Neck | 0.000260 | 0.000427 | 0.002199 | 0.004992 | 0.000585 | 0.000713 | 0.000361 | 0.003981 | 0.000396 | 0.000555 | 0.000704 | 0.004054 |
Bud Site | 0.002067 | 0.002974 | 0.004891 | 0.011560 | 0.004633 | 0.005648 | 0.002526 | 0.018370 | 0.003142 | 0.004169 | 0.002967 | 0.017877 |
Cell Periphery | 0.001026 | 0.001107 | 0.001318 | 0.002349 | 0.001466 | 0.002356 | 0.000547 | 0.006026 | 0.001210 | 0.001665 | 0.000691 | 0.005759 |
Cytoplasm | 0.041166 | 0.036934 | 0.016723 | 0.036315 | 0.058976 | 0.053176 | 0.034644 | 0.084509 | 0.048627 | 0.044194 | 0.031299 | 0.081018 |
Cytoplasmic Foci | 0.004641 | 0.006572 | 0.001416 | 0.005690 | 0.014167 | 0.015861 | 0.000605 | 0.008545 | 0.008632 | 0.010724 | 0.000756 | 0.008338 |
Eisosomes | 0.000147 | 0.000224 | 0.000034 | 0.000185 | 0.000233 | 0.000656 | 0.000048 | 0.000661 | 0.000183 | 0.000417 | 0.000045 | 0.000627 |
Endoplasmic Reticulum | 0.920911* | 0.917982* | 0.866405* | 0.838588* | 0.870918* | 0.863016* | 0.941172* | 0.808491* | 0.899967* | 0.893411* | 0.927215* | 0.810671* |
Endosome | 0.001993 | 0.003455 | 0.009692 | 0.006700 | 0.003217 | 0.006241 | 0.002303 | 0.006443 | 0.002506 | 0.004700 | 0.003683 | 0.006461 |
Golgi | 0.003718 | 0.005711 | 0.000698 | 0.001082 | 0.007293 | 0.011005 | 0.000411 | 0.005609 | 0.005216 | 0.008078 | 0.000465 | 0.005281 |
Lipid Particles | 0.004832 | 0.005142 | 0.005710 | 0.017047 | 0.009308 | 0.012435 | 0.001084 | 0.019658 | 0.006707 | 0.008402 | 0.001948 | 0.019469 |
Mitochondria | 0.003102 | 0.002990 | 0.000063 | 0.000249 | 0.005467 | 0.003892 | 0.000433 | 0.002129 | 0.004093 | 0.003393 | 0.000364 | 0.001993 |
Mitotic Spindle | 0.000096 | 0.000548 | 0.000586 | 0.006922 | 0.000040 | 0.001344 | 0.000179 | 0.000498 | 0.000073 | 0.000904 | 0.000255 | 0.000963 |
None | 0.004047 | 0.002551 | 0.000020 | 0.004773 | 0.008360 | 0.001613 | 0.001180 | 0.003558 | 0.005854 | 0.002132 | 0.000964 | 0.003646 |
Nuclear Periphery | 0.001105 | 0.001176 | 0.015323 | 0.000732 | 0.001597 | 0.001841 | 0.002491 | 0.000362 | 0.001311 | 0.001473 | 0.004887 | 0.000388 |
Nuclear Periphery Foci | 0.003679 | 0.004196 | 0.053595 | 0.019085 | 0.003796 | 0.007006 | 0.005919 | 0.007168 | 0.003728 | 0.005452 | 0.014819 | 0.008031 |
Nucleolus | 0.000131 | 0.000029 | 0.000006 | 0.000017 | 0.000082 | 0.000273 | 0.000009 | 0.000079 | 0.000111 | 0.000138 | 0.000008 | 0.000074 |
Nucleus | 0.002762 | 0.001570 | 0.001425 | 0.002069 | 0.002714 | 0.003202 | 0.002578 | 0.005925 | 0.002742 | 0.002299 | 0.002363 | 0.005646 |
Peroxisomes | 0.001126 | 0.000678 | 0.000034 | 0.000277 | 0.002715 | 0.001790 | 0.000066 | 0.001060 | 0.001792 | 0.001175 | 0.000060 | 0.001003 |
Vacuole | 0.001924 | 0.003074 | 0.019270 | 0.037816 | 0.002705 | 0.005172 | 0.002347 | 0.012607 | 0.002251 | 0.004012 | 0.005506 | 0.014433 |
Vacuole Periphery | 0.000782 | 0.001589 | 0.000275 | 0.000116 | 0.001281 | 0.001237 | 0.000117 | 0.000941 | 0.000991 | 0.001432 | 0.000146 | 0.000882 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.65 | 0.81 | -1.91 | -1.53 | -2.01 | -2.44 | -1.22 | -5.13 | -2.63 | -3.19 | -2.88 | -1.09 | -5.36 | -3.55 | -3.77 |
Bud Neck | -0.74 | -2.45 | -1.73 | -1.67 | -0.97 | -0.62 | 0.63 | -5.91 | -5.75 | -6.19 | -1.03 | -1.96 | -6.57 | -6.19 | -5.59 |
Bud Site | -1.16 | -1.10 | -2.48 | -2.26 | -1.53 | -0.73 | 1.52 | -6.21 | -5.57 | -7.08 | -1.36 | 0.00 | -7.50 | -6.88 | -7.07 |
Cell Periphery | -0.49 | -0.84 | -2.79 | -2.63 | -1.82 | -1.73 | 4.35 | -8.29 | -5.21 | -10.35 | -1.84 | 3.46 | -9.22 | -7.71 | -10.24 |
Cytoplasm | 1.51 | 6.44 | 0.40 | -0.02 | -1.92 | 1.43 | 7.24 | -5.65 | -6.48 | -11.31 | 1.86 | 7.17 | -8.25 | -9.10 | -12.10 |
Cytoplasmic Foci | -1.84 | 4.72 | -0.58 | 0.21 | -1.96 | -0.83 | 9.44 | 3.06 | 3.64 | -8.68 | -1.90 | 11.03 | -0.08 | 1.64 | -8.81 |
Eisosomes | -1.10 | 3.62 | -0.85 | 0.31 | -2.94 | -1.46 | 4.56 | -4.32 | -0.03 | -6.38 | -1.73 | 4.85 | -4.98 | -1.33 | -6.56 |
Endoplasmic Reticulum | 0.54 | 4.44 | 3.37 | 3.25 | 1.14 | 0.98 | -10.26 | 7.24 | 5.91 | 16.45 | 1.39 | -5.13 | 12.11 | 10.83 | 14.80 |
Endosome | -4.32 | -5.72 | -3.76 | -2.59 | 1.60 | -4.59 | 1.23 | -6.10 | -0.32 | -5.37 | -6.29 | -2.27 | -8.60 | -3.33 | -3.82 |
Golgi | -2.23 | 7.00 | 5.76 | 5.61 | -1.51 | -2.46 | 8.22 | 1.39 | 3.48 | -5.61 | -3.45 | 10.91 | -0.05 | 2.54 | -5.61 |
Lipid Particles | -0.39 | -0.58 | -2.45 | -2.38 | -2.18 | -1.97 | 7.91 | -5.16 | -3.33 | -10.32 | -2.04 | 7.37 | -7.36 | -6.15 | -10.13 |
Mitochondria | 0.14 | 6.00 | 5.39 | 4.14 | -1.19 | 1.77 | 8.35 | 4.67 | 2.28 | -4.13 | 1.16 | 9.45 | 3.93 | 2.36 | -4.30 |
Mitotic Spindle | -1.46 | -1.16 | -1.24 | -1.16 | -1.14 | -2.19 | -1.59 | -3.34 | 1.45 | -1.22 | -2.62 | -1.79 | -2.10 | -0.05 | -1.41 |
None | 1.86 | 6.56 | -0.18 | -0.56 | -1.21 | 5.27 | 5.61 | 3.36 | -2.45 | -2.99 | 5.26 | 7.13 | 2.31 | -1.90 | -3.45 |
Nuclear Periphery | -0.23 | -4.49 | 1.61 | 1.58 | 4.61 | -0.52 | -1.40 | 3.45 | 4.84 | 3.91 | -0.61 | -4.65 | 4.88 | 5.52 | 6.01 |
Nuclear Periphery Foci | -0.97 | -8.62 | -3.71 | -3.59 | 4.78 | -3.49 | -2.47 | -3.66 | -0.28 | -1.18 | -3.42 | -8.24 | -5.12 | -3.00 | 4.33 |
Nucleolus | 1.31 | 1.60 | 1.45 | 1.26 | -1.36 | -1.19 | 3.53 | 0.00 | 1.20 | -7.50 | -0.32 | 2.19 | 0.72 | 0.85 | -7.74 |
Nucleus | 2.53 | 2.03 | 0.73 | -0.75 | -0.74 | -0.84 | 0.08 | -4.86 | -4.05 | -4.64 | 1.14 | 0.58 | -5.01 | -6.04 | -5.07 |
Peroxisomes | 2.32 | 6.92 | 3.98 | 2.17 | -1.83 | 2.00 | 7.10 | 4.27 | 2.68 | -8.47 | 2.72 | 9.48 | 3.78 | 1.09 | -8.70 |
Vacuole | -1.88 | -5.84 | -3.53 | -3.42 | -1.81 | -2.57 | 0.41 | -8.45 | -5.64 | -8.84 | -3.24 | -5.09 | -9.47 | -7.83 | -6.42 |
Vacuole Periphery | -1.83 | 2.51 | 3.48 | 3.67 | 2.00 | 0.15 | 6.52 | 1.28 | 1.02 | -4.25 | -1.62 | 6.23 | 0.46 | 1.82 | -4.09 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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