Standard name
Human Ortholog
Description Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p; SSO1 has a paralog, SSO2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 0.63 0.53 0.28 0.37 0.66 0.65 0.76 – 0.74 0.71 0.78 0.56 0.46 0.64 0.23 0.23 0.19 0 0.11 0.08 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0.12 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.12 0.19 0.15 0.57 0.28 0.05 0 0 – 0 0 0 0.16 0.34 0.25 0.41 0.45 0.42 0.4 0.31 0.37 0.35 0.32 0.31
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.05 0.2 0.09 0.24 0.29 0.38 0.33 – 0.32 0.46 0.39 0 0 0 0.06 0 0 0.15 0.1 0.15 0.13 0.15 0.12
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.22 0.1 0.05 0 0 – 0 0 0 0.1 0.13 0.06 0.07 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.26 0.11 0.1 0.26 0.15 0.11 0 – 0.06 0.08 0 0.11 0.1 0.07 0.53 0.57 0.62 0.33 0.35 0.23 0.35 0.36 0.39
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 1 0 0 – 0 0 1 1 2 1 0 1 1 2 8 3 1 2 4
Bud 5 0 0 3 8 3 5 9 – 13 7 6 0 2 1 1 1 1 1 4 2 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 – 1 0 0 3 2 3 0 0 0 0 4 1 0 1 0
Bud Site 0 0 0 0 1 0 0 1 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 – 1 0 0 1 2 1 0 0 0 0 0 0 0 0 0
Cytoplasm 81 176 65 36 71 158 172 223 – 265 165 225 75 90 172 47 61 40 5 41 15 8 8 9
Endoplasmic Reticulum 1 6 4 0 1 3 2 0 – 0 0 1 16 8 4 11 11 7 1 3 4 3 4 4
Endosome 16 53 18 73 54 11 7 3 – 6 3 1 21 66 67 83 117 86 70 112 73 62 78 72
Golgi 0 2 0 0 1 0 0 0 – 0 0 0 0 0 1 1 2 1 1 4 1 3 7 3
Mitochondria 25 14 25 11 47 69 101 98 – 114 106 113 0 1 0 12 8 4 26 36 30 23 35 27
Nucleus 1 2 0 0 1 1 1 3 – 1 2 1 1 1 1 4 7 3 0 1 0 0 0 0
Nuclear Periphery 1 0 2 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 1 1 1 1 0 1
Nucleolus 1 0 1 3 3 1 0 1 – 0 0 0 0 1 0 2 8 1 0 0 0 0 0 0
Peroxisomes 0 0 3 6 3 0 0 1 – 0 0 0 0 4 1 1 0 0 1 1 1 3 3 2
SpindlePole 3 3 12 28 19 11 2 3 – 5 1 1 13 26 17 15 10 13 3 7 8 2 3 4
Vac/Vac Membrane 18 74 14 13 50 36 29 11 – 23 19 8 14 20 18 108 149 128 58 125 45 61 87 90
Unique Cell Count 135 281 123 127 193 240 266 294 359 232 288 133 194 269 204 260 207 177 365 197 177 241 232
Labelled Cell Count 154 331 144 173 259 294 320 353 429 303 357 145 225 287 285 375 285 177 365 197 177 241 232


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.8 4.2 4.0 4.1 3.7 3.3 3.4 – 3.2 3.1 3.0 5.8 5.4 5.5 6.8 6.6 6.7 6.3 6.5 6.8
Std Deviation (1e-4) 1.3 1.0 1.9 1.4 1.5 1.2 1.2 1.3 – 1.2 1.1 0.9 1.1 1.2 1.3 1.3 1.2 1.4 1.9 1.5 2.2
Intensity Change (Log2) – – – -0.08 -0.05 -0.16 -0.35 -0.31 – -0.41 -0.45 -0.47 0.47 0.37 0.39 0.71 0.66 0.69 0.6 0.63 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.5 8.5 9.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6307 2.3083 1.2815 0.6172 0.2769 1.3935 2.4381 3.3149 2.9424 2.2325 2.7231 3.1085 -0.2615 1.2243 0.5712 0.459 0.0104 0.6989
Actin 0.0125 0.0003 0.0191 0.0001 0.0204 0.0002 0.0023 0.0001 0.0094 0.0244 0.0015 0.0023 0.0005 0.0001 0.0023 0 0 0.0001
Bud 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Bud Neck 0.0011 0.0023 0.0027 0.0003 0.0002 0.0008 0.0015 0.0003 0.0005 0.0007 0.0002 0.0005 0.0003 0.0004 0.0008 0.0002 0.0003 0.0004
Bud Periphery 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0
Bud Site 0.0036 0.0003 0.0011 0.0002 0.0002 0.0002 0.0007 0.0002 0.0006 0.0017 0.0002 0.0002 0.0003 0.0011 0.001 0.0001 0.0001 0.0001
Cell Periphery 0.0002 0.0002 0.0005 0.0002 0 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0.0006 0.0002 0.0001 0 0.0001
Cytoplasm 0.0104 0.0032 0.0042 0.009 0.0066 0.0023 0.0093 0.0046 0.0047 0.011 0.0054 0.0041 0.0069 0.0109 0.0088 0.0022 0.0025 0.0026
Cytoplasmic Foci 0.1128 0.1064 0.1071 0.0696 0.0844 0.0846 0.0946 0.0847 0.1131 0.1484 0.1468 0.0884 0.0905 0.0779 0.1077 0.1361 0.1101 0.0827
Eisosomes 0.0001 0.0001 0.0005 0 0.0001 0.0001 0.0001 0 0.0001 0.0002 0 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0026 0.0013 0.0019 0.0019 0.0006 0.0009 0.0016 0.0014 0.0028 0.001 0.0024 0.0016 0.0018 0.0016 0.0016 0.0004 0.0003 0.0006
Endosome 0.2662 0.2811 0.2684 0.4204 0.2801 0.2787 0.3967 0.3377 0.33 0.4814 0.3884 0.2796 0.3697 0.2968 0.3501 0.524 0.3822 0.2614
Golgi 0.0216 0.0296 0.0343 0.0386 0.0329 0.0243 0.0334 0.0354 0.0458 0.0573 0.0314 0.0341 0.024 0.0227 0.0385 0.0302 0.0323 0.0282
Lipid Particles 0.448 0.4899 0.4158 0.2706 0.4557 0.4912 0.3618 0.3972 0.367 0.2005 0.338 0.4741 0.4066 0.4799 0.3592 0.1848 0.395 0.5247
Mitochondria 0.0073 0.0024 0.0097 0.0359 0.0048 0.0062 0.009 0.0047 0.006 0.0074 0.0073 0.0064 0.0069 0.0022 0.0173 0.0442 0.0022 0.004
None 0.0332 0.0156 0.041 0.062 0.0336 0.0173 0.0078 0.0408 0.0236 0.0062 0.0072 0.0163 0.0199 0.0124 0.0196 0.0005 0.0004 0.0122
Nuclear Periphery 0.003 0.0027 0.0085 0.0034 0.0007 0.004 0.0073 0.0028 0.0047 0.0016 0.0092 0.0142 0.0048 0.0061 0.0073 0.0007 0.0004 0.0082
Nucleolus 0.0039 0.0031 0.0025 0.0007 0.0003 0.0025 0.0087 0.0019 0.0061 0.0013 0.0006 0.0031 0.0022 0.0051 0.0027 0.0006 0.0008 0.001
Nucleus 0.0013 0.0004 0.0021 0.001 0.0003 0.0009 0.0086 0.0013 0.0018 0.0005 0.0008 0.0013 0.0024 0.0051 0.003 0.0003 0.0003 0.0005
Peroxisomes 0.0331 0.0158 0.0432 0.0151 0.0577 0.0274 0.0264 0.0171 0.0403 0.0314 0.0274 0.0255 0.0164 0.0109 0.0339 0.017 0.0408 0.0146
Punctate Nuclear 0.0045 0.0024 0.0104 0.001 0.0019 0.0032 0.0052 0.0009 0.0105 0.0022 0.0032 0.0038 0.005 0.0051 0.0046 0.001 0.0011 0.0022
Vacuole 0.0269 0.0298 0.0189 0.0546 0.0159 0.0406 0.0189 0.0572 0.0272 0.0166 0.0189 0.0295 0.0338 0.051 0.0328 0.0438 0.0249 0.041
Vacuole Periphery 0.0074 0.0129 0.0077 0.0151 0.0035 0.0144 0.0056 0.0115 0.0053 0.0056 0.011 0.0145 0.0075 0.0099 0.0083 0.0136 0.0061 0.0153

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.0363 55.3636 36.8901 73.0141 81.1172 56.845 45.982 45.1064 60.7747 84.6003
Translational Efficiency 1.6759 1.0003 2.0115 1.5321 1.79 2.1394 1.2057 1.3178 1.8899 1.6859

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1829 1836 1041 494 1633 1605 2717 1766 3462 3441 3758 2260

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.17 761.53 779.66 783.95 677.24 705.80 836.32 863.57 663.47 735.54 820.62 846.17
Standard Deviation 86.29 120.78 97.09 118.93 99.65 95.31 107.53 123.91 93.74 113.11 107.77 127.17
Intensity Change Log 2 — 0.225867 0.259811 0.267727 — 0.059592 0.304388 0.350646 — 0.143493 0.282709 0.310595

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000190 0.000292 0.000631 0.001122 0.000213 0.000351 0.001105 0.004081 0.000201 0.000320 0.000974 0.003434
Bud Neck 0.015457 0.013975 0.018874 0.011081 0.014533 0.016002 0.025284 0.017940 0.015021 0.014920 0.023509 0.016441
Bud Site 0.002698 0.006033 0.007955 0.015986 0.003760 0.004579 0.017003 0.044389 0.003199 0.005355 0.014496 0.038181
Cell Periphery 0.000116 0.000065 0.000169 0.000157 0.000085 0.000123 0.000105 0.000147 0.000101 0.000092 0.000123 0.000149
Cytoplasm 0.210097* 0.170327* 0.171411* 0.322069* 0.133455* 0.086283 0.136085* 0.298915* 0.173946* 0.131126* 0.145871* 0.303976*
Cytoplasmic Foci 0.354019* 0.338914* 0.113041 0.139550 0.345331* 0.303410* 0.190280* 0.162888 0.349921* 0.322354* 0.168884 0.157787
Eisosomes 0.000241 0.000109 0.000129 0.000098 0.000214 0.000168 0.000065 0.000130 0.000228 0.000136 0.000083 0.000123
Endoplasmic Reticulum 0.003339 0.004169 0.006196 0.001869 0.003902 0.004381 0.002452 0.001888 0.003605 0.004268 0.003489 0.001884
Endosome 0.121085 0.167562 0.162323 0.077403 0.215453* 0.266698* 0.204742* 0.057757 0.165597 0.213802* 0.192991 0.062051
Golgi 0.010911 0.015883 0.001294 0.007980 0.024458 0.026553 0.006314 0.004984 0.017301 0.020860 0.004924 0.005639
Lipid Particles 0.098856 0.057050 0.056085 0.012377 0.102573* 0.086116 0.042789 0.015719 0.100609* 0.070607 0.046472 0.014989
Mitochondria 0.012696 0.011151 0.003074 0.002405 0.010451 0.028818 0.006427 0.005047 0.011637 0.019391 0.005498 0.004469
Mitotic Spindle 0.002700 0.012000 0.013647 0.028012 0.004215 0.007591 0.092980 0.049227 0.003415 0.009944 0.071004 0.044590
None 0.002663 0.000979 0.007131 0.014420 0.002011 0.001108 0.001377 0.002453 0.002356 0.001039 0.002971 0.005069
Nuclear Periphery 0.001793 0.001856 0.011444 0.002007 0.001797 0.001297 0.006879 0.002210 0.001795 0.001595 0.008143 0.002165
Nuclear Periphery Foci 0.001896 0.003626 0.028513 0.006291 0.002405 0.001924 0.010514 0.003461 0.002136 0.002832 0.015500 0.004079
Nucleolus 0.003558 0.002699 0.026484 0.002889 0.002847 0.002269 0.008768 0.001359 0.003223 0.002499 0.013676 0.001693
Nucleus 0.048705 0.027659 0.199334* 0.085481 0.021363 0.015529 0.041340 0.069832 0.035808 0.022001 0.085106 0.073253
Peroxisomes 0.015187 0.012731 0.012682 0.019493 0.015082 0.017497 0.044567 0.053611 0.015137 0.014954 0.035735 0.046153
Vacuole 0.090175 0.147050 0.155938 0.247227* 0.090871 0.116982 0.158606 0.201975* 0.090503 0.133025 0.157867 0.211866*
Vacuole Periphery 0.003618 0.005870 0.003646 0.002084 0.004982 0.012321 0.002318 0.001989 0.004262 0.008879 0.002686 0.002010

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.24 -1.24 -3.42 -2.81 -0.41 -1.90 -5.81 -6.35 -5.95 -4.39 -2.85 -4.98 -6.71 -6.32 -4.38
Bud Neck 1.03 -2.01 3.70 3.00 4.66 -0.97 -9.26 -2.18 -0.88 6.61 0.04 -8.92 -0.67 -0.67 7.51
Bud Site -4.33 -3.59 -2.86 -1.67 -0.97 -1.11 -8.77 -11.87 -11.53 -6.84 -3.97 -10.09 -12.42 -11.19 -6.99
Cell Periphery 8.55 -4.07 -0.18 -3.00 2.22 -2.46 -1.25 -4.39 0.14 -1.84 1.11 -1.86 -2.59 -2.83 -0.35
Cytoplasm 5.13 4.70 -6.00 -8.74 -8.50 7.68 -2.09 -18.20 -25.86 -17.33 8.42 4.03 -16.05 -22.64 -19.15
Cytoplasmic Foci 2.33 35.52 28.21 26.51 0.19 5.96 26.10 30.36 24.47 7.57 5.71 40.65 41.96 36.80 6.22
Eisosomes 13.83 10.53 14.39 1.58 4.83 2.66 12.93 7.86 4.03 -7.39 9.77 19.79 14.04 3.39 -4.54
Endoplasmic Reticulum -1.82 -6.67 3.27 4.37 9.61 -0.83 3.06 3.87 4.42 1.66 -1.78 0.06 5.22 6.35 7.01
Endosome -7.61 -4.04 10.07 15.75 11.35 -6.50 5.58 28.67 35.17 27.35* -9.48 -0.90 29.62 38.53 29.37*
Golgi -3.97 13.55 3.39 5.40 -1.32 -1.16 12.36 13.57 15.13 2.70 -3.04 15.16 14.20 16.90 1.05
Lipid Particles 9.83 9.12 23.58 16.18 12.14 2.94 14.87 22.13 19.79 13.13 8.82 18.89 32.21 25.24 17.53
Mitochondria 1.07 7.22 7.62 9.00 2.22 -8.31 4.10 6.02 12.07 3.89 -5.84 7.25 8.78 13.96 4.07
Mitotic Spindle -6.50 -5.98 -4.88 -2.65 -1.89 -1.99 -22.48* -11.63 -10.53 9.85 -6.16 -23.18 -12.99 -10.36 8.12
None 4.09 -3.74 -2.63 -3.35 -0.78 1.60 1.06 0.02 -2.00 -1.33 3.77 -1.36 -1.74 -4.03 -0.83
Nuclear Periphery -0.33 -11.46 -3.10 -3.14 10.12 0.97 -8.82 -2.59 -5.50 7.83 0.56 -13.91 -3.87 -5.53 11.52
Nuclear Periphery Foci -3.52 -10.84 -4.73 -3.54 6.92 1.38 -9.43 -3.33 -4.59 6.05 -1.90 -14.57 -5.99 -4.70 9.52
Nucleolus 1.41 -10.30 -0.39 -1.54 9.99 1.64 -8.70 1.87 0.36 10.58 2.11 -13.25 2.10 0.17 14.51
Nucleus 7.81 -18.08 -5.88 -9.24 10.70 3.16 -10.62 -17.04 -18.99 -8.53 8.15 -16.80 -14.67 -19.84 1.75
Peroxisomes 2.09 1.81 0.89 -0.10 -0.14 -1.76 -11.32 -7.72 -6.60 0.62 0.20 -10.44 -7.28 -7.34 -0.00
Vacuole -11.45 -9.84 -18.11 -13.64 -12.52 -5.07 -16.54 -25.88 -21.21 -13.50 -11.88 -20.17 -32.21 -24.13 -18.06
Vacuole Periphery -5.12 0.75 5.56 9.20 4.48 -9.54 6.44 7.11 16.28 2.35 -10.67 6.88 9.11 18.48 4.33
Standard name
Human Ortholog
Description Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p; SSO1 has a paralog, SSO2, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (27%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sso1

Sso1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sso1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available